公开(公告)号：US20200074624A1

公开(公告)日：2020-03-05

申请号：US16557376

申请日：2019-08-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey MARKS ,  David WOO ,  Wallace GEORGE

IPC: G06T7/00 ,  H04N5/372 ,  H04N5/347 ,  G01N27/447

Abstract: A biological analysis system and an associated method are provided. The method is used for recovering off scale data in an image produced by a camera in a capillary electrophoresis instrument. The method comprises the steps of identifying bins of the image where electron counts exceed a maximum number of counts; setting an off-scale flag for the identified bins; and processing the image to obtain a recovered dye signal, based on the flag set for each bin, and using a dye matrix.

公开(公告)号：US20220013193A1

公开(公告)日：2022-01-13

申请号：US17312168

申请日：2019-12-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yong CHU ,  Stephanie SCHNEIDER ,  Rylan SCHAEFFER ,  David WOO

IPC: G16B40/20 ,  G16B30/20 ,  C12Q1/6869 ,  G06N3/08

Abstract: A deep basecaller system for Sanger sequencing and associated methods are provided. The methods use deep machine learning. A Deep Learning Model is used to determine scan labelling probabilities based on an analyzed trace. A Neural Network is trained to learn the optimal mapping function to minimize a Connectionist Temporal Classification (CTC) Loss function. The CTC function is used to calculate loss by matching a target sequence and predicted scan labelling probabilities. A Decoder generates a sequence with the maximum probability. A Basecall position finder using prefix beam search is used to walk through CTC labelling probabilities to find a scan range and then the scan a position of peak labelling probability within the scan range for each called base. Quality Value (QV) is determined using a feature vector calculated from CTC labelling probabilities as an index into a QV look-up table to find a quality score.

公开(公告)号：US20190170684A1

公开(公告)日：2019-06-06

申请号：US16265806

申请日：2019-02-01

Applicant: Life Technologies Corporation

Inventor： David DENNY ,  David WOO ,  Manjula ALIMINATI ,  Siva Kumar SAMSANI ,  Stephanie SCHNEIDER ,  Yoke Peng LIM ,  Sylvia CHANG

IPC: G01N27/447 ,  G16B30/00

CPC classification number: G01N27/44717 ,  G16B30/00

Abstract: In one exemplary embodiment, a method for detecting variants in electropherogram data is provided. The method includes receiving electropherogram data from an instrument and analyzing the electropherogram data to identify mixed bases in the electropherogram data. The method further includes validating the identified mixed bases. Then the method includes determining variants in the electropherogram data based on the validated mixed bases.

公开(公告)号：US20190126281A1

公开(公告)日：2019-05-02

申请号：US16116801

申请日：2018-08-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yong CHU ,  Jeffrey MARKS ,  Jacob FREUDENTHAL ,  Mingsong CHEN ,  Tiong Han TOH ,  Mauro AGUANNO ,  Lik Seng LAU ,  Lian Seng LOH ,  Kok Siong TEO ,  Zeng Wei CHU ,  Xin MATHERS ,  Michael UY ,  Huei Steven YEO ,  Kuan Moon BOO ,  Way Xuang LEE ,  Chin Yong KOO ,  Wei Fuh TEO ,  Soo Yong LAU ,  Hon Siu SHIN ,  Zeqi TAN ,  Thomas WESSEL ,  David WOO

IPC: B01L7/00 ,  G01N21/27 ,  G01N21/64 ,  C12Q1/6806 ,  G01N35/02

Abstract: A biological analysis system is provided. The system comprises a sample block assembly. The sample block assembly comprises a sample block configured to accommodate a sample holder, the sample holder configured to receive a plurality of samples. The system also comprises a control system configured to cycle the plurality of samples through a series of temperatures. The system further comprises an automated tray comprising a slide assembly, the tray configured to reversibly slide the sample block assembly from a closed to an open position to allow user access to the plurality of sample holders.