公开(公告)号：US20210310024A1

公开(公告)日：2021-10-07

申请号：US17046693

申请日：2019-04-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Maya YOVCHEVA ,  Jonathan ZMUDA ,  Sara BARNES ,  Chang CHOI ,  Kenneth THOMPSON

IPC: C12N15/86 ,  C12N5/07 ,  C12N7/00 ,  C12P21/00

Abstract: The present disclosure is related generally to systems and methods for high level expression of recombinant proteins from baculovirus in insect cells. In particular, the methods and systems described herein allow for high levels of baculovirus production in insect cells and/or high levels of protein production in insect cells using a chemically-defined, yeast lysate-free insect cell medium. The disclosure also relates to compositions and kits for culturing, transfecting, and/or producing recombinant protein in insect cells.

公开(公告)号：US20170016043A1

公开(公告)日：2017-01-19

申请号：US15209695

申请日：2016-07-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan ZMUDA ,  Chao Yan LIU

IPC: C12P21/02 ,  C07K14/505 ,  C07K16/00

CPC classification number: C12P21/02 ,  C07K14/505 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/92 ,  C12N5/0043 ,  C12N2510/02 ,  C12N2511/00

Abstract: The present invention is directed generally to systems and methods suitable for high level expression of recombinant proteins in suspension CHO cells. In particular, the invention allows introduction of the invention obviates the need to replace, replenish or supplement the growth medium during the procedure. The invention also relates to compositions and kits useful for culturing and transforming/transfecting suspension CHO cells.

Abstract translation: 本发明一般涉及适用于悬浮CHO细胞中重组蛋白高水平表达的系统和方法。 特别地，本发明允许本发明的引入消除了在该过程期间更换，补充或补充生长培养基的需要。 本发明还涉及可用于培养和转化/转染悬浮液CHO细胞的组合物和试剂盒。

公开(公告)号：US20240368564A1

公开(公告)日：2024-11-07

申请号：US18778386

申请日：2024-07-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xin YU ,  Xavier de MOLLERAT du JEU ,  Chao Yan LIU ,  Jonathan ZMUDA ,  Jian LIU

IPC: C12N7/00 ,  C12N1/06 ,  C12N15/86

Abstract: The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2×1011 viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.

公开(公告)号：US20240218308A1

公开(公告)日：2024-07-04

申请号：US18399270

申请日：2023-12-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Samantha STEPNOWSKI ,  Jonathan ZMUDA ,  Keith WHITTLINGER ,  Babak HAMIDIAN

IPC: C12M1/24

CPC classification number: C12M23/08

Abstract: A cell culture flask having a flask body that defines a cavity for receiving a cell culture medium is provided. The flask body includes an elongate neck that defines an opening to the cavity, a base, and a sidewall that extends from the elongate neck to the base of the flask body. The sidewall includes a circumferential beveled portion, which may or may not be faceted, that extends from an outermost periphery of the flask body to the base of the flask body at a constant angle relative to a central axis of the flask to define a sidewall angle.

公开(公告)号：US20220195478A1

公开(公告)日：2022-06-23

申请号：US17563665

申请日：2021-12-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan ZMUDA ,  Chao Yan LIU

IPC: C12P21/02 ,  C12N5/00 ,  C07K14/505 ,  C07K16/00

Abstract: The present invention is directed generally to systems and methods suitable for high level expression of recombinant proteins in suspension CHO cells. In particular, the invention allows introduction of the invention obviates the need to replace, replenish or supplement the growth medium during the procedure. The invention also relates to compositions and kits useful for culturing and transforming/transfecting suspension CHO cells.