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    SYSTEM AND METHOD FOR HIGH-YIELD TRANSIENT EXPRESSION IN MAMMALIAN CELLS
    2.
    发明申请
    SYSTEM AND METHOD FOR HIGH-YIELD TRANSIENT EXPRESSION IN MAMMALIAN CELLS 有权

    公开(公告)号：US20230130038A1

    公开(公告)日：2023-04-27

    申请号：US17977788

    申请日：2022-10-31

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Sanjay K. VASU ,  Chao Yan LIU ,  Jeffrey ROGERS ,  Maria Isabel CISNEROS ,  Jingqui LI ,  Henry C. CHIOU ,  Meredith Bauman JONES

    IPC: C07K14/505 ,  C12N5/00 ,  C12N5/0793

    Abstract: High-yield mammalian transient expression systems can include a cell culture media (particularly serum free, non-animal derived, and/or chemically defined media) for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). Cells containing such introduced materials can then be cultured in the cell culture media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly mammalian cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

    HIGH YIELD TRANSIENT EXPRESSION IN MAMMALIAN CELLS USING UNIQUE PAIRING OF HIGH DENSITY GROWTH AND TRANSFECTION MEDIUM AND EXPRESSION ENHANCERS
    3.
    发明申请
    HIGH YIELD TRANSIENT EXPRESSION IN MAMMALIAN CELLS USING UNIQUE PAIRING OF HIGH DENSITY GROWTH AND TRANSFECTION MEDIUM AND EXPRESSION ENHANCERS 审中-公开
    使用高密度生长和转移介质和表达增强剂的独特配对在马马里兰细胞中的高瞬态表达

    公开(公告)号：US20130316400A1

    公开(公告)日：2013-11-28

    申请号：US13886226

    申请日：2013-05-02

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Sanjay VASU ,  Henry CHIOU ,  Jeffrey ROGERS ,  Maria Isabel CISNEROS ,  Jingqiu LI ,  Chao Yan LIU ,  Meredith JONES

    IPC: C07K14/505

    CPC classification number: C07K14/505 ,  C12N5/005 ,  C12N5/0619 ,  C12N2500/30 ,  C12N2501/065 ,  C12N2501/999 ,  C12N2510/02 ,  C12N2511/00

    Abstract: The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or chemically defined media) which are useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). According to the invention, such introduction can take place in the presence of said medium. Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the cells can be measured or determined. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

    Abstract translation: 本发明一般涉及可用于将大分子和化合物（例如核酸分子）引入细胞（例如真核细胞）中的细胞培养基（特别是无血清，非动物衍生的和/或化学上限定的培养基）。 根据本发明，这种引入可以在所述介质的存在下进行。 然后可以在培养基中培养含有这种引入的物质的细胞，并且可以测量或测定引入的细胞对细胞的影响。 特别地，本发明允许将核酸分子（例如，载体）引入细胞（特别是真核细胞）中，以及由细胞中核酸分子编码的蛋白质的表达。 本发明消除了在每次用细胞（例如，培养细胞与转染细胞）进行不同程序时改变细胞培养基的需要。 本发明还涉及可用于培养和转化/转染细胞的组合物和试剂盒。

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