公开(公告)号：US20240263196A1

公开(公告)日：2024-08-08

申请号：US18398730

申请日：2023-12-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jian LIU ,  Neha PARAYATH ,  Evgenia VEROVSKAYA

IPC: C12N15/88 ,  C12N9/22 ,  C12N15/11

CPC classification number: C12N15/88 ,  C12N9/22 ,  C12N15/11 ,  C12N2310/20

Abstract: Provided herein, are methods, compositions, and kits for introducing a payload to an immune cell. In aspects, a lipid composition is described, which includes at least one ionizable lipid compound having the structure I. In aspects, the methods and compositions are for introducing a nucleic acid payload into immune cells, for example T cells, dendritic cells, and NK cells.

公开(公告)号：US20240368564A1

公开(公告)日：2024-11-07

申请号：US18778386

申请日：2024-07-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xin YU ,  Xavier de MOLLERAT du JEU ,  Chao Yan LIU ,  Jonathan ZMUDA ,  Jian LIU

IPC: C12N7/00 ,  C12N1/06 ,  C12N15/86

Abstract: The instant technology relates to a production system to produce AAV vectors in a serum free suspension platform and at high titers. This technology uses reagents comprising media, cells, transfection reagent, AAV enhancer, and a lysis buffer, each of which is designed to provide maximal AAV production from suspension culture of mammalian cells, e.g. HEK293 cells. With this new system we are able to deliver up to about 2×1011 viral genomes per milliliter (vg/mL) of unconcentrated AAV vectors.