公开(公告)号：US20180245116A1

公开(公告)日：2018-08-30

申请号：US15873477

申请日：2018-01-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xiquan LIANG ,  Chang-Ho BAEK ,  Lansha PENG ,  Federico KATZEN

IPC: C12P19/34 ,  C12N15/66 ,  C12N15/64 ,  C12N15/10

CPC classification number: C12P19/34 ,  C12N15/10 ,  C12N15/1031 ,  C12N15/64 ,  C12N15/66

Abstract: The present disclosure generally relates to compositions and methods for the assembly of nucleic acid molecules into larger nucleic acid molecules. Also provided are compositions and methods for seamlessly connection of nucleic acid molecules with high sequence fidelity.

公开(公告)号：US20240247286A1

公开(公告)日：2024-07-25

申请号：US18374673

申请日：2023-09-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xiquan LIANG ,  Robert Jason POTTER ,  Lansha PENG

IPC: C12N15/90 ,  C07K14/195 ,  C12N9/22 ,  C12N15/63

CPC classification number: C12N15/907 ,  C07K14/195 ,  C12N9/22 ,  C12N15/63 ,  C12N2800/80 ,  C12N2800/95 ,  C12N2810/40

Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.

公开(公告)号：US20190119701A1

公开(公告)日：2019-04-25

申请号：US16124931

申请日：2018-09-07

Applicant: Life Technologies Corporation

Inventor： Xiquan LIANG ,  Robert Jason POTTER ,  Lansha PENG

IPC: C12N15/90 ,  C07K14/195 ,  C12N9/22

Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.