公开(公告)号：US20170107539A1

公开(公告)日：2017-04-20

申请号：US15294586

申请日：2016-10-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xin YU ,  Xiquan LIANG ,  Xavier de MOLLERAT du JEU ,  Robert Jason POTTER

IPC: C12N15/90 ,  C12N9/22

CPC classification number: C12N15/90 ,  A61K48/0033 ,  A61K48/005 ,  C12N9/22 ,  C12N15/88 ,  C12N2830/30 ,  C12Y301/00

Abstract: Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.

公开(公告)号：US20210054415A1

公开(公告)日：2021-02-25

申请号：US17093486

申请日：2020-11-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xin YU ,  Xiquan LIANG ,  Xavier de MOLLERAT du JEU ,  Robert Jason POTTER

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/88 ,  A61K48/00

Abstract: Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.

公开(公告)号：US20190390229A1

公开(公告)日：2019-12-26

申请号：US16095147

申请日：2017-04-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert Jason POTTER ,  Jason CARTER

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22 ,  C12P19/34

Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents. Further disclosed are nucleic acid compositions with reduced cytotoxicity.

公开(公告)号：US20240247286A1

公开(公告)日：2024-07-25

申请号：US18374673

申请日：2023-09-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xiquan LIANG ,  Robert Jason POTTER ,  Lansha PENG

IPC: C12N15/90 ,  C07K14/195 ,  C12N9/22 ,  C12N15/63

CPC classification number: C12N15/907 ,  C07K14/195 ,  C12N9/22 ,  C12N15/63 ,  C12N2800/80 ,  C12N2800/95 ,  C12N2810/40

Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.

公开(公告)号：US20190119701A1

公开(公告)日：2019-04-25

申请号：US16124931

申请日：2018-09-07

Applicant: Life Technologies Corporation

Inventor： Xiquan LIANG ,  Robert Jason POTTER ,  Lansha PENG

IPC: C12N15/90 ,  C07K14/195 ,  C12N9/22

Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.

公开(公告)号：US20180327738A1

公开(公告)日：2018-11-15

申请号：US15777182

申请日：2016-11-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Namritha RAVINDER ,  Robert Jason POTTER ,  Jason CARTE

IPC: C12N15/10 ,  C12N9/22

CPC classification number: C12N15/1003 ,  C07K2319/80 ,  C07K2319/81 ,  C12N9/22

Abstract: The present disclosure generally relates to compositions and methods for genetic modification of cells. In particular, the disclosure relates to stabilized reagents for genome alteration (e.g., site specific nucleases), as well as stabilized reagents for non-genetic code altering modification (e.g., DNA methylation). Stabilization methods include storage of reagents at suitable temperatures, conversion to dry forms, and chemical modifications.