公开(公告)号：US20180010177A1

公开(公告)日：2018-01-11

申请号：US15695244

申请日：2017-09-05

Applicant: Life Technologies Corporation

Inventor： Stephen HENDRICKS ,  David KING ,  Lei XI ,  Marian PERIS

IPC: C12Q1/68 ,  C12Q1/46 ,  C12P19/34

CPC classification number: C12Q1/46 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

公开(公告)号：US20170081706A1

公开(公告)日：2017-03-23

申请号：US15282597

申请日：2016-09-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Lei XI ,  Paul KENNEY ,  Zhaochun MA ,  Dennis PROSEN ,  Stephen HENDRICKS ,  Roland NAGEL

IPC: C12Q1/68 ,  C12N9/12 ,  C07H21/02

CPC classification number: C12Q1/686 ,  C07H21/00 ,  C07H21/02 ,  C12N9/1252 ,  C12N15/10 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.

公开(公告)号：US20190024159A1

公开(公告)日：2019-01-24

申请号：US16154529

申请日：2018-10-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen HENDRICKS ,  David KING ,  Lei XI ,  Marian PERIS

IPC: C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6874 ,  C12Q1/6844 ,  C12Q1/46 ,  C12P19/34

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

公开(公告)号：US20150191775A1

公开(公告)日：2015-07-09

申请号：US14593858

申请日：2015-01-09

Applicant: Life Technologies Corporation

Inventor： Lei XI ,  Paul Kenney ,  Zhaochun Ma ,  Dennis Prosen ,  Stephen Hendricks ,  Roland Nagel

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C07H21/00 ,  C07H21/02 ,  C12N9/1252 ,  C12N15/10 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.

Abstract translation: 本公开涉及使用多磷酸盐，多磷酸盐，亚氨基二磷酸盐，硫代磷酸盐（或μ-一硫代焦磷酸盐）和相关化合物中的至少一种进行多磷酸解（APP）反应进行活化的方法。

公开(公告)号：US20210285026A1

公开(公告)日：2021-09-16

申请号：US17211102

申请日：2021-03-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Lei XI ,  Keith P. BJORNSON ,  Stephen P. HENDRICKS

IPC: C12Q1/686 ,  C12Q1/6853

Abstract: This specification generally relates to non-radioactive methods of detecting nucleic acid polymerase activity and methods of detecting compounds that modulate nucleic acid polymerase activity. The activity may be measured in real-time using a real-time PCR instrument.