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公开(公告)号:US20210032691A1
公开(公告)日:2021-02-04
申请号:US16949083
申请日:2020-10-13
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Bin LI , Kai Qin LAO , Jennifer O'NEIL , Jennifer KUNKEL , Kellie HALEY , Rachel KASINSKAS , Zhaochun MA , Pius BRZOSKA
IPC: C12Q1/6853 , C12Q1/6844 , C12Q1/6806 , C12P19/34 , C12Q1/6869 , C12Q1/6874 , C12Q1/6834 , C12Q1/46
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
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2.CLONAL AMPLIFICATION OF NUCLEIC ACID ON SOLID SURFACE WITH TEMPLATE WALKING 有权
Title translation: 核酸在固体表面的克隆放大与模板运动公开(公告)号:US20150275284A1
公开(公告)日:2015-10-01
申请号:US14692706
申请日:2015-04-21
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Bin LI , Kai Qin LAO , Jennifer O'NEIL , Jennifer KUNKEL , Kellie HALEY , Rachel KASINSKAS , Zhaochun MA , Pius BRZOSKA
IPC: C12Q1/68
CPC classification number: C12Q1/46 , C12P19/34 , C12Q1/6834 , C12Q1/6846 , C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2531/119 , C12Q2565/537
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
Abstract translation: 提供了在载体上产生固定化克隆扩增子的局部群体的新方法。
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3.METHODS, COMPOSITIONS, SYSTEMS, APPARATUSES AND KITS FOR NUCLEIC ACID AMPLIFICATION 有权
Title translation: 方法,组成,系统,核酸放大的装置和工具公开(公告)号:US20130203607A1
公开(公告)日:2013-08-08
申请号:US13828049
申请日:2013-03-14
Applicant: Life Technologies Corporation
Inventor: Bin LI , Kai Qin LAO , Jennifer O'NEIL , Jennifer KUNKEL , Kellie HALEY , Rachel KASINSKAS , Zhaochun MA , Pius BRZOSKA
IPC: C12Q1/68
CPC classification number: C12Q1/6853 , C12Q1/6874 , C12Q2521/531 , C12Q2525/117 , C12Q2535/119 , C12Q2523/313
Abstract: In some embodiments, the present teachings provide methods for paired end sequencing. In some embodiment, a polynucleotide template to be subjected to paired end sequencing comprises at least one cross linking moiety and at least one scissile moiety. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cleavage step, and (c) a reverse sequencing step. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cross-linking step, (c) a cleavage step, and (d) a reverse sequencing step.
Abstract translation: 在一些实施方案中,本教导提供了用于配对末端测序的方法。 在一些实施方案中,待进行配对末端测序的多核苷酸模板包含至少一个交联部分和至少一个可剪切部分。 在一些实施方案中,配对末端测序反应包括(a)正向测序步骤,(b)切割步骤,和(c)反向测序步骤。 在一些实施方案中,配对末端测序反应包括(a)正向测序步骤,(b)交联步骤,(c)切割步骤和(d)反向测序步骤。
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公开(公告)号:US20230175051A1
公开(公告)日:2023-06-08
申请号:US18104548
申请日:2023-02-01
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Bin Li , Kai Qin LAO , Jennifer O'NEIL , Jennifer KUNKEL , Kellie HALEY , Rachel KASINSKAS , Zhaochun MA , Pius BRZOSKA
IPC: C12Q1/6853 , C12Q1/6844 , C12Q1/6806 , C12P19/34 , C12Q1/6869 , C12Q1/6874 , C12Q1/6834 , C12Q1/46
CPC classification number: C12Q1/6853 , C12Q1/6846 , C12Q1/6806 , C12P19/34 , C12Q1/6869 , C12Q1/6874 , C12Q1/6834 , C12Q1/46 , C12Q2531/119 , C12Q2565/537
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
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公开(公告)号:US20190264272A1
公开(公告)日:2019-08-29
申请号:US16351194
申请日:2019-03-12
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Bin LI , Kai Lao , Jennifer O'NEIL , Jennifer KUNKEL , Kellie HALEY , Rachel KASINSKAS , Zhaochun MA , Pius BRZOSKA
IPC: C12Q1/6853 , C12Q1/6834 , C12Q1/6874 , C12Q1/46 , C12Q1/6844 , C12Q1/6869 , C12P19/34
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
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6.
公开(公告)号:US20170081706A1
公开(公告)日:2017-03-23
申请号:US15282597
申请日:2016-09-30
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Lei XI , Paul KENNEY , Zhaochun MA , Dennis PROSEN , Stephen HENDRICKS , Roland NAGEL
CPC classification number: C12Q1/686 , C07H21/00 , C07H21/02 , C12N9/1252 , C12N15/10 , C12Q1/6869 , C12Y207/07007
Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.
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7.CLONAL AMPLIFICATION OF NUCLEIC ACID ON SOLID SURFACE WITH TEMPLATE WALKING 审中-公开
Title translation: 核酸在固体表面的克隆放大与模板运动公开(公告)号:US20170067098A1
公开(公告)日:2017-03-09
申请号:US15276713
申请日:2016-09-26
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Bin LI , Kai Qin LAO , Jennifer O'NEIL , Jennifer KUNKEL , Kellie HALEY , Rachel KASINSKAS , Zhaochun MA , Pius BRZOSKA
CPC classification number: C12Q1/6853 , C12P19/34 , C12Q1/46 , C12Q1/6834 , C12Q1/6846 , C12Q1/6869 , C12Q1/6874 , C12Q2531/119 , C12Q2565/537
Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
Abstract translation: 提供了在载体上产生固定化克隆扩增子的局部群体的新方法。
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8.Synthesis Of 2',3'-Dideoxynucleosides For Automated DNA Synthesis And Pyrophosphorolysis Activated Polymerization 有权
Title translation: 用于自动DNA合成和焦磷酸解活化聚合的2',3'-二脱氧核苷的合成公开(公告)号:US20160108079A1
公开(公告)日:2016-04-21
申请号:US14952149
申请日:2015-11-25
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Zhaochun MA , Khairuzzaman Bashar MULLAH , Robert G. EASON
IPC: C07H19/16
CPC classification number: C07H19/16 , C07D239/54 , C07D405/04 , C07D473/18 , C07D473/34 , C07H19/06 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/6806
Abstract: Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
Abstract translation: 公开了制备2',3'-二脱氧核苷酸支持结构的方法,例如2',3'-二脱氧鸟苷,2',3'-二脱氧腺苷和3'-脱氧胸苷支持结构。 还提供了使用这些结构的各种方法,例如其用于自动DNA合成和焦磷酸裂解活化聚合的用途。
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9.Synthesis Of 2' , 3' -Dideoxynucleosides For Automated DNA Synthesis And Pyrophosphorolysis Activated Polymerization 有权
Title translation: 用于自动DNA合成和焦磷酸分解活化聚合的2',3'-二脱氧核苷的合成公开(公告)号:US20150045547A1
公开(公告)日:2015-02-12
申请号:US14173725
申请日:2014-02-05
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Zhaochun MA , Khairuzzaman Bashar Mullah , Robert Eason
CPC classification number: C07H19/16 , C07D239/54 , C07D405/04 , C07D473/18 , C07D473/34 , C07H19/06 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/6806
Abstract: Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
Abstract translation: 公开了制备2',3'-二脱氧核苷酸支持结构的方法,例如2',3'-二脱氧鸟苷,2',3'-二脱氧腺苷和3'-脱氧胸苷支持结构。 还提供了使用这些结构的各种方法,例如其用于自动DNA合成和焦磷酸裂解活化聚合的用途。
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