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    Rapid lateral flow assay for determining exposure to Mycobacterium tuberculosis and other mycobacteria
    1.
    发明授权
    Rapid lateral flow assay for determining exposure to Mycobacterium tuberculosis and other mycobacteria 失效
    用于确定暴露于结核分枝杆菌和其他分枝杆菌的快速侧流测定

    公开(公告)号：US06841159B2

    公开(公告)日：2005-01-11

    申请号：US10061036

    申请日：2002-01-30

    申请人： Lloyd G. Simonson

    发明人： Lloyd G. Simonson

    IPC分类号： G01N33/558 ,  G01N33/569 ,  A61K39/04 ,  A61K9/02

    CPC分类号： G01N33/558 ,  G01N33/5695 ,  G01N2469/20

    摘要： An assay method and kit is disclosed for detecting the presence of at least one predesignated, target antibody to a mycobacterium in a sample selected from one or more patient bodily fluids. The method comprises the following steps: (a) contacting the sample of one or more patient bodily fluids with at least one mycobacterium antigen on a lateral-flow assay membrane to bind to the target antibody in the sample; (b) previously, simultaneously or subsequently to step (a), binding the at least one mycobacterium antigen with a conjugated label producing a detectable signal; and (c) detecting the signal whereby the presence of the target antibody is determined in the sample by the intensity or presence of the signal. The method can further comprise the step of evaluating immunization status of the patient from whom the sample came by comparing the signal or lack thereof with immunizations previously received by the patient and in comparison to a known standard control. In a preferred embodiment, the mycobacterium antigen specifically binds to Mycobacterium tuberculosis specific antibodies. Preferably, the immunoassay of the present invention comprises a lateral-flow assay comprising a membrane, a conjugated label pad, and at least one mycobacterium antigen bound to the membrane. In a preferred embodiment, the at least one mycobacterium antigen is selected from the group consisting of 38 kDa and 16 kDa antigens.

    摘要翻译： 公开了用于检测在选自一种或多种患者体液中的样品中存在至少一种预定名的分枝杆菌目标抗体的测定方法和试剂盒。 该方法包括以下步骤：（a）将一种或多种患者体液的样品与侧流测定膜上的至少一种分枝杆菌抗原接触以结合样品中的靶抗体; （b）先前，同时或随后的步骤（a），将所述至少一种分枝杆菌抗原与产生可检测信号的共轭标记结合; 并且（c）检测信号，由此通过信号的强度或存在在样品中确定靶抗体的存在。 该方法可以进一步包括通过将信号或其缺乏与先前接收的患者的免疫接种并与已知的标准对照相比较来评估患者的免疫状态的步骤。 在优选的实施方案中，分枝杆菌抗原特异性结合结核分枝杆菌特异性抗体。 优选地，本发明的免疫测定包括横向流动测定，其包括膜，缀合的标签垫和与膜结合的至少一种分枝杆菌抗原。 在优选的实施方案中，所述至少一种分枝杆菌抗原选自38kDa和16kDa抗原。

    Production of monoclonal antibodies to treponema denticola by hybridoma TDIII, IIIBB2
    2.
    发明授权
    Production of monoclonal antibodies to treponema denticola by hybridoma TDIII, IIIBB2 失效
    通过杂交瘤TDIII，IIIBB2生产针对密螺旋体denticola的单克隆抗体

    公开(公告)号：US4959304A

    公开(公告)日：1990-09-25

    申请号：US355575

    申请日：1989-05-22

    申请人： Lloyd G. Simonson

    发明人： Lloyd G. Simonson

    IPC分类号： G01N33/569

    CPC分类号： G01N33/56955

    摘要： A monoclonal antibody is disclosed which is reactive to Treponema denticoland produced by the hybridoma deposited under ATCC HB 9967. The invention also disclosed diagnostic reagents and methods for detecting Treponema denticola utilizing the hybridoma deposited under ATCC HB 9967.

    摘要翻译： 公开了一种单克隆抗体，其对锥虫的反应性并由由ATCC HB 9967保藏的杂交瘤产生。本发明还公开了利用保藏在ATCC HB 9967下的杂交瘤检测梅毒螺旋体的诊断试剂和方法。

    Method of producing a plaque dispersing enzyme
    3.
    发明授权
    Method of producing a plaque dispersing enzyme 失效
    生产斑块分散酶的方法

    公开(公告)号：US4328313A

    公开(公告)日：1982-05-04

    申请号：US232595

    申请日：1981-02-09

    申请人： Lloyd G. Simonson ,  Burton L. Lamberts

    发明人： Lloyd G. Simonson ,  Burton L. Lamberts

    IPC分类号： A61K38/00 ,  C12N9/24

    CPC分类号： C12Y302/01071 ,  C12N9/2402 ,  C12Y302/01084 ,  A61K38/00 ,  Y10S435/874

    摘要： A method of producing .alpha.-1, 3-glucanase by introducing a bacterial culture such as Pseudomonas sp. isolate NRRL B-12324 into an aqueous medium containing a "limit glucan" substrate which is greater than 90 percent .alpha.-1, 3-glycosidically linked, then allowing growth to take place to accumulate .alpha.-1, 3-glucanase, and then recovering the enzyme for use as an oral therapeutic agent.

    摘要翻译： 通过引入细菌培养物如假单胞菌属（Pseudomonas sp。）来产生α-1,3-葡聚糖酶的方法。 将NRRL B-12324分离成含有“极性葡聚糖”底物的水性培养基，其大于90％的α-1,3-糖苷连接，然后允许生长以积累α-1,3-葡聚糖酶，然后回收 用作口服治疗剂的酶。

    Production of monoclonal antibodies to Treponema denticola by hybridoma TDII, IAA11
    5.
    发明授权
    Production of monoclonal antibodies to Treponema denticola by hybridoma TDII, IAA11 失效
    通过杂交瘤TDII，IAA11生产针对梅毒螺旋体的单克隆抗体

    公开(公告)号：US5514553A

    公开(公告)日：1996-05-07

    申请号：US356044

    申请日：1989-05-22

    申请人： Lloyd G. Simonson

    发明人： Lloyd G. Simonson

    IPC分类号： C07K16/12 ,  G01N33/569 ,  G01N33/53

    CPC分类号： G01N33/56955 ,  C07K16/1207

    摘要： A monoclonal antibody is disclosed which is reactive to Treponema denticola and produced by the hybridoma deposited under ATCC HB 9966. The invention also discloses diagnostic reagents and methods for detecting Treponema denticola utilizing the hybridoma deposited under ATCC HB 9966.

    摘要翻译： 公开了一种单克隆抗体，其可以针对梅毒螺旋体感染并由由ATCC HB 9966保藏的杂交瘤产生。本发明还公开了利用ATCC HB 9966保藏的杂交瘤检测梅毒螺旋体的诊断试剂和方法。

    Production of monoclonal antibodies to bacteroides gingivalis by hybridoma BGII, VF9/2D
    8.
    发明授权
    Production of monoclonal antibodies to bacteroides gingivalis by hybridoma BGII, VF9/2D 失效
    通过杂交瘤BGII，VF9 / 2D生产牙龈卟啉单胞菌的单克隆抗体

    公开(公告)号：US5665559A

    公开(公告)日：1997-09-09

    申请号：US356899

    申请日：1989-05-18

    申请人： Lloyd G. Simonson

    发明人： Lloyd G. Simonson

    IPC分类号： C07K16/12 ,  G01N33/569 ,  G01N33/577

    CPC分类号： G01N33/56911 ,  C07K16/1257

    摘要： A monoclonal antibody is disclosed which is reactive to Bacteroides gingivalis and produced by the hybridoma deposited under ATCC HB 9968. The invention also discloses diagnostic reagents and methods for detecting Bacteroides gingivalis utilizing the hybridoma deposited under ATCC HB 9968.

    摘要翻译： 公开了一种单克隆抗体，其对牙龈卟啉单胞菌具有反应活性，并由保藏在ATCC HB 9968上的杂交瘤产生。本发明还公开了利用保藏在ATCC HB 9968上的杂交瘤检测牙龈卟啉菌的诊断试剂和方法。

    Rapid and non-invasive method to evaluate immunization status of a patient
    10.
    发明授权
    Rapid and non-invasive method to evaluate immunization status of a patient 失效
    快速和非侵入性的方法来评估患者的免疫状态

    公开(公告)号：US06927068B2

    公开(公告)日：2005-08-09

    申请号：US10060605

    申请日：2002-01-30

    申请人： Lloyd G. Simonson ,  John R. Kelly

    发明人： Lloyd G. Simonson ,  John R. Kelly

    IPC分类号： G01N33/558 ,  G01N33/53

    CPC分类号： G01N33/558 ,  G01N2333/32 ,  Y10S435/97 ,  Y10S436/81

    摘要： An assay method and kit for detecting the presence of a predesignated, target IgG antibody in a sample selected from one or more patient bodily fluids. The method comprises the following steps: (a) contacting the sample of one or more patient bodily fluids with a membrane-bound recombinant protective antigen to bind to the target IgG antibody in the sample; (b) previously, simultaneously or subsequently to step (a), binding the protective antigen (PA) with a conjugated label producing a detectable signal; and (c) detecting the signal whereby the presence of the target IgG antibody is determined in the sample by the intensity of the signal. The method can further comprise the step of evaluating immunization status of the patient from whom the sample came by comparing the signal or lack thereof with immunizations previously received by the patient. In a preferred embodiment, the recombinant protective antigen (PA) specifically binds to anthrax protective antigen-specific IgG antibodies. Preferably, the immunoassay of the present invention comprises a lateral-flow assay comprising a membrane, a conjugated label pad, and a recombinant protective antigen (PA) bound to the membrane.

    摘要翻译： 用于检测选自一种或多种患者体液的样品中预先指定的目标IgG抗体的存在的测定方法和试剂盒。 该方法包括以下步骤：（a）使一种或多种患者体液的样品与膜结合的重组保护性抗原接触，以与样品中的目标IgG抗体结合; （b）先前同时或随后的步骤（a），将保护性抗原（PA）与产生可检测信号的共轭标记物结合; 和（c）检测信号，由此通过信号的强度在样品中确定目标IgG抗体的存在。 该方法可以进一步包括通过将信号或其缺乏与患者以前接收的免疫接种进行比较来评估患者来自患者的免疫状态的步骤。 在优选的实施方案中，重组保护性抗原（PA）特异性结合炭疽保护性抗原特异性IgG抗体。 优选地，本发明的免疫测定包括横向流动测定，其包括膜，缀合的标签垫和与膜结合的重组保护性抗原（PA）。