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16 条记录 (耗时 0.023 秒)

    Process and device for detecting microparticle movement
    1.
    发明授权
    Process and device for detecting microparticle movement 失效
    用于检测微粒运动的方法和装置

    公开(公告)号：US06467328B1

    公开(公告)日：2002-10-22

    申请号：US09555068

    申请日：2000-07-24

    申请人： Markus Kalkum ,  Markus Kietzmann ,  Martin Müller

    发明人： Markus Kalkum ,  Markus Kietzmann ,  Martin Müller

    IPC分类号： G01N100

    CPC分类号： B01L3/0241 ,  G01N2035/00237 ,  G01N2035/1034

    摘要： For the detection of the position and/or velocity of microparticles which move from dispensers of a dispensing head (30) to a target, a detector target with two linear bound, electro-acoustic or electro-optic interactive areas which fix a target plane is used. When microparticles impact on the interactive areas, detector signals are determined from which along with the dispensing head position motion parameters of the microparticles are obtained.

    摘要翻译： 为了检测从分配头（30）的分配器移动到目标的微粒的位置和/或速度，具有固定目标平面的两个线性束缚，电声或电光交互区域的检测器目标是 用过的。 当微粒冲击交互区域时，确定检测器信号与其一起获得微粒的分配头位置运动参数。

    Procedure and device for acoustically detecting microparticles
    4.
    发明授权
    Procedure and device for acoustically detecting microparticles 失效
    用于声学检测微粒的方法和装置

    公开(公告)号：US06561005B2

    公开(公告)日：2003-05-13

    申请号：US09755742

    申请日：2001-01-05

    申请人： Martin Müller ,  Rudolf Germer ,  Markus Kalkum ,  Holger Eickhoff

    发明人： Martin Müller ,  Rudolf Germer ,  Markus Kalkum ,  Holger Eickhoff

    IPC分类号： G01P2500

    CPC分类号： G01N29/046 ,  G01N2291/015 ,  G01N2291/02408

    摘要： A procedure for detecting microparticles released by at least one dispenser, encompassing the following steps: (a) triggering the dispenser over an oscillation target (20) with a sensor material (21), so that at least one microparticle hits the sensor material (21) and excites an acoustic wave, (b) detecting the acoustic wave with at least one oscillation sensor (31) connected with the sensor material (21), which emits an electrical sensor signal when the acoustic wave arrives, and (c) evaluating the sensor signal to determine the impact of the microparticle. The procedure is aimed at detecting the impact and/or impact positions of microparticles on a target. A detector for executing the procedure is also described.

    摘要翻译： 一种用于检测由至少一个分配器释放的微粒的方法，包括以下步骤：（a）用传感器材料（21）将分配器触发在振荡目标（20）上，使得至少一个微粒撞击传感器材料（21 ）并激发声波，（b）用至少一个与传感器材料（21）连接的振荡传感器（31）检测声波，当声波到达时发出电传感器信号，以及（c）评估 传感器信号来确定微粒的影响。 该程序旨在检测微粒对靶标的影响和/或影响位置。 还描述了用于执行该过程的检测器。

    Methods for detection of botulinum neurotoxin
    7.
    发明授权
    Methods for detection of botulinum neurotoxin 有权
    肉毒杆菌神经毒素检测方法

    公开(公告)号：US08067192B2

    公开(公告)日：2011-11-29

    申请号：US12134092

    申请日：2008-06-05

    申请人： Markus Kalkum ,  Karine Bagramyan

    发明人： Markus Kalkum ,  Karine Bagramyan

    IPC分类号： G01N33/573

    CPC分类号： G01N33/573 ,  C07K7/08 ,  C07K14/705 ,  G01N33/5308 ,  G01N33/542 ,  G01N33/569 ,  G01N2333/32 ,  G01N2333/33 ,  G01N2333/38

    摘要： Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as botulinum neurotoxin, bacillus anthracis lethal factor, human chitinases, and aspergillus fumigatus proteases. Also provided are methods for constructing and identifying novel luminescent or fluorescent substrates suitable for use with the ALISSA method.

    摘要翻译： 本文提供了用于快速和灵敏检测毒素或酶活性的大型免疫吸附剂表面积测定（ALISSA）。 该测定被设计为捕获低数量的毒素或酶分子，并通过荧光或发光底物的转化来测量它们的内在蛋白酶活性。 ALISSA比本领域常规使用的方法显着更快和更灵敏。 该测定适用于检测各种具有蛋白水解活性的毒素或酶，如肉毒杆菌神经毒素，炭疽杆菌致死因子，人几丁质酶和烟曲霉蛋白酶。 还提供了用于构建和鉴定适用于ALISSA方法的新型发光或荧光底物的方法。

    Methods for detection of botulinum neurotoxin
    8.
    发明授权
    Methods for detection of botulinum neurotoxin 有权
    肉毒杆菌神经毒素检测方法

    公开(公告)号：US08753831B2

    公开(公告)日：2014-06-17

    申请号：US13600186

    申请日：2012-08-30

    申请人： Markus Kalkum ,  Karine Bagramyan

    发明人： Markus Kalkum ,  Karine Bagramyan

    IPC分类号： G01N21/76 ,  G01N21/64 ,  G01N33/53

    CPC分类号： G01N33/5308 ,  C07K7/08 ,  C07K14/705 ,  G01N33/5306 ,  G01N33/542 ,  G01N33/569 ,  G01N33/573 ,  G01N2333/32 ,  G01N2333/33 ,  G01N2333/38 ,  G01N2333/90241

    摘要： Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.

    摘要翻译： 本文提供了用于快速和灵敏检测肉毒杆菌神经毒素（BoNT）和炭疽毒素的大型免疫吸附剂表面积测定（ALISSA）。 该测定设计用于捕获少量的毒素分子并通过转化荧光底物或发光底物测量其内在的蛋白酶活性。 本文还提供了可以被BoNT特异性切割的新型肽和对BoNT切割具有抗性的新型肽。 这些可切割和对照肽的组合可用于实施用于特异性检测BoNT酶活性的示例性ALISSA。 此外，如本文所述的ALISSA也可以以基于列的格式使用，以用于高通量系统中用于测试大量样品。

    METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN
    10.
    发明申请
    METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN 有权
    检测嗜碱性粒细胞毒素的方法

    公开(公告)号：US20090176259A1

    公开(公告)日：2009-07-09

    申请号：US12134092

    申请日：2008-06-05

    申请人： Markus Kalkum ,  Karine Bagramyan

    发明人： Markus Kalkum ,  Karine Bagramyan

    IPC分类号： G01N33/573 ,  G01N33/569

    CPC分类号： G01N33/573 ,  C07K7/08 ,  C07K14/705 ,  G01N33/5308 ,  G01N33/542 ,  G01N33/569 ,  G01N2333/32 ,  G01N2333/33 ,  G01N2333/38

    摘要： Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorigenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as botulinum neurotoxin, bacillus anthracis lethal factor, human chitinases, and aspergillus fumigatus proteases. Also provided are methods for constructing and identifying novel luminescent or fluorescent substrates suitable for use with the ALISSA method.

    摘要翻译： 本文提供了用于快速和灵敏检测毒素或酶活性的大型免疫吸附剂表面积测定（ALISSA）。 该测定被设计为捕获少量的毒素或酶分子，并通过转化荧光或发光底物来测量它们的内在蛋白酶活性。 ALISSA比本领域常规使用的方法显着更快和更灵敏。 该测定适用于检测各种具有蛋白水解活性的毒素或酶，如肉毒杆菌神经毒素，炭疽杆菌致死因子，人几丁质酶和烟曲霉蛋白酶。 还提供了用于构建和鉴定适用于ALISSA方法的新型发光或荧光底物的方法。