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1.发明授权Novel restriction endonuclease SplI and process for the production of the same 失效新型限制性内切核酸酶SplI及其生产过程相同
公开(公告)号：US4886756A
公开(公告)日：1989-12-12
申请号：US758536
申请日：1985-07-24
申请人： Masahide Kawamura , Masaki Sakakibara , Teruo Watanabe , Akira Obauashi , Nobutsugu Hiraoka , Keiko Kita
发明人： Masahide Kawamura , Masaki Sakakibara , Teruo Watanabe , Akira Obauashi , Nobutsugu Hiraoka , Keiko Kita
CPC分类号： C12N9/22
摘要： A novel restriction endonuclease SplI which has the following physicochemical properties:(1) recognizing the following base sequences in double-stranded deoxyribonucleic acid ##STR1## and cleaving said sequences in the phosphodiester bonds between C and G as indicated with the vertical arrows to produce DNA fragments having one strand comprising four bases at the 5'-terminal;(2) cleaving double-stranded deoxyribonucleic acid .lambda.-DNA in one position, Col El in two positions and .phi.x 174 RF in two positions;(3) being activated with 5 to 20 mM Mg.sup.2+ ; and(4) exhibiting an activity at a NaCl concentration of 0 to 200 mM;and a process for the production of the restriction endonuclease SplI which comprises culturing a restriction endonuclease SplI-producing alga belonging to the genus Spirulina, collecting the cells, obtaining a cell-free extract therefrom the separating and purifying the restriction endonuclease SplI.
摘要翻译： 具有以下物理化学性质的新型限制性内切核酸酶SplI：（1）在双链脱氧核糖核酸中识别以下碱基序列，并用垂直箭头所示切割C和G之间的磷酸二酯键中的所述序列，以产生DNA 具有在5'末端具有4个碱基的一条链的片段; （2）在一个位置切割双链脱氧核糖核酸λ-DNA，两个位置分别为Col El，两个位置为174个RF; （3）用5〜20mM Mg 2+活化; 和（4）在NaCl浓度为0至200mM下表现出活性; 以及生产限制性内切核酸酶SplI的方法，其包括培养属于螺旋藻属的限制性内切核酸酶SplI产生藻类，收集细胞，从其中获得分离和纯化限制性内切核酸酶SplI的无细胞提取物。
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公开(公告)号：US4668631A
公开(公告)日：1987-05-26
申请号：US777674
申请日：1985-09-19
CPC分类号： C12N9/22 , Y10S435/822
摘要： A restriction endonuclease having the ability to recognize the same base sequence and cleavage sites as SacII and SstII can be produced from Gluconobacter and isolated in pure form because no other restriction enzyme is formed.
摘要翻译： 具有识别与SacII和SstII相同的碱基序列和切割位点的能力的限制性内切核酸酶可由葡糖杆菌产生并以纯形式分离，因为不形成其它限制酶。
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公开(公告)号：US4808531A
公开(公告)日：1989-02-28
申请号：US756380
申请日：1985-07-18
CPC分类号： C12N9/22 , Y10S435/822
摘要： The invention provides restriction endonuclease Mf1 I capable of recognizing the base sequence as shown below on a double-stranded DNA molecule and cleaving the DNA chain at the arrow-marked positions, but has no such action when A is methylated5'--Pu.dwnarw.GATC Py--3'3'--Py CTAG.uparw.Pu--5'(wherein A represents adenosine, G guanosine, T thymidine, C cytidine, Pu adenosine or guanosine, and Py thymidine or cytidine). The restriction endonuclease is produced by culturing Microbacterium flavum IAM 1642, FERM BP-938 in a culture medium and recovering it from the culture.
摘要翻译： 本发明提供了限制性内切核酸酶Mf1I，其能够识别如下所示的碱基序列在双链DNA分子上并且在箭头标记位置处切割DNA链，但当A是甲基化5'-Pu＆darr＆GATC时没有这样的作用 Py-3'3'-Py CTAG＆uarr＆Pu-5'（其中A表示腺苷，G鸟苷，T胸苷，C胞苷，Pu腺苷或鸟苷，Py胸苷或胞苷）。 限制性内切酶通过在培养基中培养黄色微生物IAM1642，FERM BP-938并从培养物中回收来产生。
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公开(公告)号：US4724209A
公开(公告)日：1988-02-09
申请号：US783634
申请日：1985-10-03
CPC分类号： C12N9/22 , Y10S435/822
摘要： A process for producing a restriction endonuclease capable of recognizing the nucleotide sequence ##STR1## (wherein A, C, G and T represent adenosine, cytidine, guanosine and thymidine, respectively) on a DNA chain and specifically cleaving the double-stranded chain at the arrow-marked positions. This process comprises growing a microorganism belonging to the genus Halococcus and capable of producing said restriction endonuclease, and collecting the enzyme thus formed from the culture broth.
摘要翻译： （其中A，C，G和T分别代表腺苷，胞苷，鸟苷和胸腺嘧啶核苷酸）分别限定在DNA链上并特异性切割双链的限制性内切核酸酶的方法 链在箭头标记的位置。 该方法包括生长属于卤虫属的微生物并且能够产生所述限制性内切核酸酶，并收集由培养液形成的酶。
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5.发明授权RNA polymerase gene, microorganism having said gene and the production of RNA polymerase by the use of said microorganism 失效RNA聚合酶基因，具有所述基因的微生物和通过使用所述微生物产生RNA聚合酶
公开(公告)号：US5026645A
公开(公告)日：1991-06-25
申请号：US109615
申请日：1987-10-19
CPC分类号： C12N9/1247
摘要： SP6 bacteriophage RNA polymerase is produced by cultivating a new microorganism (particularly new strains of Escherichia coli) harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene and recovering SP6 bacteriophage RNA polymerase from the culture broth. SP6 bacteriophage RNA polymerase gene is provided as are new microorganisms harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene.
摘要翻译： 通过培养携带SP6噬菌体RNA聚合酶基因的质粒并从培养液中回收SP6噬菌体RNA聚合酶的新的微生物（特别是大肠杆菌的新菌株）产生SP6噬菌体RNA聚合酶。 提供了SP6噬菌体RNA聚合酶基因，其含有携带SP6噬菌体RNA聚合酶基因的质粒的新微生物。
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公开(公告)号：US5470732A
公开(公告)日：1995-11-28
申请号：US688426
申请日：1991-04-22
CPC分类号： C12N9/22 , Y10S435/84 , Y10S435/843
摘要： A restriction enzyme is obtained by cultivating a strain of Brevibacterium linens and recovering the restriction enzyme. The restriction enzyme, which has the same specificity as AvrII, cleaves the following nucleotide sequence specifically at the arrow-marked sites: ##STR1##
摘要翻译： 通过培养短杆菌亚麻菌株并回收限制酶获得限制酶。 与AvrII具有相同特异性的限制性酶特异性地在箭头标记的位点切割以下核苷酸序列：
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公开(公告)号：US4868115A
公开(公告)日：1989-09-19
申请号：US72843
申请日：1987-07-09
CPC分类号： C12N9/96
摘要： An enzyme composition comprising a series of enzymes used for DNA base sequence analysis by gel electrophoresis and a dihydric alcohol.
摘要翻译： 一种酶组合物，其包含用于通过凝胶电泳进行DNA碱基序列分析的一系列酶和二元醇。
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