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公开(公告)号：US20080220419A1
公开(公告)日：2008-09-11
申请号：US11665843
申请日：2005-11-02
IPC分类号： C12Q1/68 , C07H21/00 , C07H1/00 , C12N15/63 , C07K14/00 , C12N15/00 , C12N9/04 , C12N1/00 , C12P7/00
CPC分类号： C12N9/0077 , C07K2319/00
摘要： The present invention provides a method for preparing a hybrid gene. The method includes a step of amplifying a P450 gene fragment contained in a sample using primers designed on the basis of regions of a plurality of P450 in which amino acid sequences are highly conserved and a step of preparing the hybrid gene using the amplified fragments and a known P450 gene. The method includes no culturing step or a step of normalizing extracted DNAs and is useful in isolating a P450 gene from various microbial resources.The present invention further provides a fused cytochrome P450 monooxygenase containing a peptide which is linked to the C-terminus of a P450 protein with a linker portion disposed therebetween and which has the same function as that of a reductase domain contained in a cytochrome P450 monooxygenase originating from Rhodococcus sp. strain NCIMB 9784. This enables the construction of a high-efficiency electron transfer system useful for various P450 proteins and also enables the production of an active P450 monooxygenase.
摘要翻译： 本发明提供了一种制备杂交基因的方法。 该方法包括使用基于其中氨基酸序列高度保守的多个P450的区域设计的引物和使用扩增片段制备杂交基因的步骤来扩增样品中包含的P450基因片段的步骤，以及 已知的P450基因。 该方法不包括培养步骤或标准化提取的DNA的步骤，并且可用于从各种微生物资源中分离P450基因。 本发明还提供一种融合的细胞色素P450单加氧酶，其含有与P450蛋白的C末端连接的肽，其中连接部位置于其间，并且具有与源于细胞色素P450单加氧酶的还原酶结构域相同的功能 来自红球菌属 应用NCIMB 9784.这使得能够构建可用于各种P450蛋白质的高效电子转移系统，并且还能够生产活性P450单加氧酶。
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2.发明授权Keto group-introducing enzyme, DNA coding therefor and method for producing ketocarotenoids 失效酮基引入酶，其编码的DNA和生产酮类胡萝卜素的方法
公开(公告)号：US5910433A
公开(公告)日：1999-06-08
申请号：US632434
申请日：1996-04-23
申请人： Susumu Kajiwara , Norihiko Misawa , Keiji Kondo
发明人： Susumu Kajiwara , Norihiko Misawa , Keiji Kondo
CPC分类号： C12N9/0004 , C12P23/00 , C12P7/26 , Y10S435/946
摘要： DNA sequences are described that encode genes for synthesizing ketocarotenoids such as astaxanthin. The DNA sequences, microorganisms containing them and encoded polypeptides are described. Also described are methods to obtain related sequences and to make host cells that contain such sequences. These genes and methods are useful to impart red coloration during culture of fish and crustaceans, as coloring in food, and as antioxidants.
摘要翻译： PCT No.PCT / JP95 / 01640 Sec。 371日期：1996年4月23日 102（e）日期1996年4月23日PCT提交1995年8月18日PCT公布。 出版物WO96 / 06172 日期1996年2月29日描述了编码用于合成酮类胡萝卜素如虾青素的基因的DNA序列。 描述了DNA序列，含有它们的微生物和编码的多肽。 还描述了获得相关序列并制备含有这种序列的宿主细胞的方法。 这些基因和方法可用于在鱼和甲壳类动物培养期间赋予红色，作为食物中的着色以及作为抗氧化剂。
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公开(公告)号：US5429939A
公开(公告)日：1995-07-04
申请号：US783705
申请日：1991-10-23
摘要： Disclosed are DNA sequences which are useful for the synthesis of carotenoids such as lycopene, .beta.-carotene, zeaxanthin or zeaxanthin-diglucoside, that is, DNA sequences encoding carotenoid biosynthesis enzymes. These DNA sequences are the sequences 1-6 shown in the specification.Also disclosed is a process for producing a carotenoid or a carotenoid related compound which is selected from the group consisting of geranylgeranyl pyrophosphate, phytoene, lycopene, .beta.-carotene, zeaxanthin and zeaxanthin-diglucoside, which comprises transforming a host with at least one of the DNA sequences 1-6 described above and culturing the transformant.
摘要翻译： 公开了可用于合成类胡萝卜素如番茄红素，β-胡萝卜素，玉米黄质或玉米黄质 - 二葡萄糖苷的DNA序列，即编码类胡萝卜素生物合成酶的DNA序列。 这些DNA序列是说明书中所示的序列1-6。 还公开了一种制备类胡萝卜素或类胡萝卜素相关化合物的方法，该化合物选自er牛儿基ger anyl酮焦磷酸盐，八氢番茄红素，番茄红素，β-胡萝卜素，玉米黄质和玉米黄质 - 二葡萄糖苷，其包括用至少一种 DNA序列1-6，并培养转化体。
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4.发明申请Method of Producing Astaxanthin or Metabolic Product Thereof by Using Carotenoid Ketolase and Carotenoid Hydroxylase Genes 有权使用类胡萝卜素酮醇酶和类胡萝卜素羟化酶基因生产虾青素或其代谢产物的方法公开(公告)号：US20090298146A1
公开(公告)日：2009-12-03
申请号：US11597362
申请日：2005-05-26
申请人： Seon-Kang Choi , Norihiko Misawa
发明人： Seon-Kang Choi , Norihiko Misawa
CPC分类号： C12N15/8243 , C12N9/0008 , C12N15/825 , C12P23/00
摘要： To provide a microorganism or a plant transformed with a β-ionone ring-4-ketolase gene and/or β-ionone ring-3-hydroxylase gene derived from Brevundimonas sp. strain SD-212. The β-ionone ring-4-ketolase gene and β-ionone ring-3-hydroxylase gene produced by Brevundimonas sp. strain SD-212 each have a high activity compared with those of known enzymes, and therefore microorganisms transformed with the genes encoding these enzymes can efficiently produce astaxanthin.
摘要翻译： 为了提供转化了来自Brevundimonas sp。的β-紫罗兰酮环-4-酮醇酶基因和/或β-紫罗兰酮环-3-羟化酶基因的微生物或植物。 菌株SD-212。 由Brevundimonas sp。生产的β-紫罗兰酮环-4-酮醇酶基因和β-紫罗兰酮环-3-羟化酶基因。 菌株SD-212各自具有比已知酶高的活性，因此用编码这些酶的基因转化的微生物可以有效地产生虾青素。
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公开(公告)号：US20080254519A1
公开(公告)日：2008-10-16
申请号：US10591603
申请日：2005-02-22
CPC分类号： C12P17/12 , C12N9/0071 , C12P17/16 , C12Y114/12018
摘要： The present invention relates to a method for producing a picolinic acid compound. Specifically, the present invention relates to a method for producing a picolinic acid compound, which comprises reacting an aromatic compound that contains a phenyl group represented by the following formula (I), (II), or (III) with aromatic ring dioxygenase, aromatic ring dihydrodiol dehydrogenase, and aromatic ring diol dioxygenase, and obtaining a picolinic acid compound (I′), (II′), or (III′). wherein, H1 is an optionally substituted heterocyclic group, A1 is a single bond or an optionally substituted C1-4 alkylene group or alkenylene group, P2 is an optionally substituted phenyl group, and C1 is an optionally substituted cyclic hydrocarbon group (excluding a phenyl group), and where formula II does not represent diphenylacetylene.
摘要翻译： 本发明涉及吡啶甲酸化合物的制造方法。 具体而言，本发明涉及一种吡啶甲酸化合物的制造方法，其特征在于，将含有下述式（I），（II）或（III）所示的苯基的芳香族化合物与芳香环双加氧酶，芳香族化合物 环二醇二氢脱氢酶和芳环二醇双加氧酶，得到吡啶甲酸化合物（I'），（II'）或（III'）。 其中，H1是任选取代的杂环基，A1是单键或任选取代的C 1-4亚烷基或亚烯基，P2是任选取代的苯基，且C1是任选取代的 环状烃基（苯基除外），式II不表示二苯基乙炔。
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6.发明申请Novel carotenoid hydroxylase gene, method for preparing hydroxylated carotenoid, and novel geranylgeranyl pyrophosphate synthase 失效新型类胡萝卜素羟化酶基因，羟基化类胡萝卜素的制备方法和新型香叶基香叶基焦磷酸合成酶公开(公告)号：US20070148727A1
公开(公告)日：2007-06-28
申请号：US10579338
申请日：2004-11-04
申请人： Yasuhiro Nishida , Kyoko Adachi , Yoshikazu Shizuri , Hiroaki Kasai , Sadao Komemushi , Norihiko Misawa
发明人： Yasuhiro Nishida , Kyoko Adachi , Yoshikazu Shizuri , Hiroaki Kasai , Sadao Komemushi , Norihiko Misawa
CPC分类号： C12P23/00 , C12N9/0071 , C12N9/1085
摘要： The present invention provides a Brevundimonas sp. strain SD212-derived peptide having β-ionone ring-2-hydroxylase activity and a gene encoding the same, to thereby make it possible to produce rare carotenoids in which a hydroxyl group is introduced at the position 2 (2′) carbon in their β-ionone ring in large quantities. The present invention also provides a novel gene encoding an enzyme which introduces a hydroxyl group at the position 3 (3′) carbon in the β-ionone ring of carotenoids, and a novel gene encoding a geranylgeranyl pyrophosphate synthase.
摘要翻译： 本发明提供了一种Brevundimonas sp。 具有β-紫罗兰酮环-2-羟化酶活性的SD212衍生肽菌株和编码该抗体的基因，从而使得可以生产在其β位的2（2'）碳上引入羟基的稀有类胡萝卜素 -ionone环大量。 本发明还提供了编码引入类胡萝卜素的β-紫罗兰酮环中的3（3'）位上的羟基的酶的新基因，以及编码香叶基香叶基焦磷酸合成酶的新基因。
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公开(公告)号：US09562220B2
公开(公告)日：2017-02-07
申请号：US14124256
申请日：2012-06-08
CPC分类号： C12P23/00 , C12N9/001 , C12N15/52 , C12P5/026 , C12Y103/99029 , C12Y205/0101 , C12Y205/01096 , Y02P20/52
摘要： The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.
摘要翻译： 本发明提供一种生产具有50个碳原子的类胡萝卜素的方法，其包括在培养基中培养用突变型八氢番茄红素去饱和酶基因转化的细胞，并从培养物中获得具有50个碳原子的类胡萝卜素。 突变体八氢番茄红素去饱和酶基因具有引入突变以编码具有增强的使50个碳原子的类胡萝卜素主链化合物去饱和的活性的突变体八氢番茄红素去饱和酶。
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公开(公告)号：US20140170700A1
公开(公告)日：2014-06-19
申请号：US14124256
申请日：2012-06-08
CPC分类号： C12P23/00 , C12N9/001 , C12N15/52 , C12P5/026 , C12Y103/99029 , C12Y205/0101 , C12Y205/01096 , Y02P20/52
摘要： The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.
摘要翻译： 本发明提供一种生产具有50个碳原子的类胡萝卜素的方法，其包括在培养基中培养用突变型八氢番茄红素去饱和酶基因转化的细胞，并从培养物中获得具有50个碳原子的类胡萝卜素。 突变体八氢番茄红素去饱和酶基因具有引入突变以编码具有增强的使50个碳原子的类胡萝卜素主链化合物去饱和的活性的突变体八氢番茄红素去饱和酶。
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9.发明授权DNA strands useful for the synthesis of xanthophylls and the process for producing the xanthophylls 有权可用于合成叶黄素的DNA链和产生叶黄素的方法
公开(公告)号：US6150130A
公开(公告)日：2000-11-21
申请号：US335919
申请日：1999-06-18
CPC分类号： C12N9/0093 , C12N9/0004 , C12P23/00
摘要： Disclosed are the following DNA strands relating to the synthesis of keto group-containing xanthophylls such as astaxanthin and the like, and the techniques relating to the production of xanthophylls by genetic engineering:A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a .beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a 3-hydroxy-.beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for adding a hydroxyl group to the 3-carbon of a 4-keto-.beta.-ionone ring.It is possible to produce a variety of xanthophylls such as canthaxanthin, astaxanthin and the like by introducing the DNA strands into an appropriate microorganism such as Escherichia coli and the like.
摘要翻译： 公开了与合成含有酮基的叶黄素如虾青素等相关的以下DNA链，以及通过基因工程与叶黄素的生产有关的技术：具有编码具有酶的多肽的核苷酸序列的DNA链 将β-丁酮环的4-位上的亚甲基转化为酮基的活性。 具有编码具有将3-羟基-β-雄酮环的4-位的亚甲基转换为酮基的酶活性的多肽的核苷酸序列的DNA链。 一种DNA链，其具有编码具有用于向4-酮-β-雄酮环的3-碳中添加羟基的酶活性的多肽的核苷酸序列。 通过将DNA链引入适当的微生物如大肠杆菌等可以产生各种叶黄素如角黄素，虾青素等。
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10.发明授权DNA strands useful for the synthesis of xanthophylls and the process for producing the xanthophylls 失效可用于合成叶黄素的DNA链和产生叶黄素的方法
公开(公告)号：US5972690A
公开(公告)日：1999-10-26
申请号：US6491
申请日：1998-01-13
CPC分类号： C12N9/0093 , C12N9/0004 , C12P23/00
摘要： Disclosed are the following DNA strands relating to the synthesis of keto group-containing xanthophylls such as astaxanthin and the like, and the techniques relating to the production of xanthophylls by genetic engineering:A DNA strand having a nucleotide sequence which encodes a polypeptides having and enzyme activity for converting a methylene group at the 4-position of a .beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for converting a methylene group at the 4-position of a 3-hydroxy-.beta.-ionone ring into a keto group.A DNA strand having a nucleotide sequence which encodes a polypeptide having an enzyme activity for adding a hydroxyl group to the 3-carbon of a 4-keto-.beta.-ionone ring.It is possible to produce a variety of xanthophylls such as canthaxanthin, astaxanthin and the like by introducing the DNA strands into an appropriate microorganism such as Escherichia coli and the like.
摘要翻译： 公开了与合成含有酮基的叶黄素如虾青素等有关的以下DNA链，以及通过遗传工程生产叶黄素的技术：具有编码具有和酶的多肽的核苷酸序列的DNA链 将β-丁酮环的4-位上的亚甲基转化为酮基的活性。 具有编码具有将3-羟基-β-雄酮环的4-位的亚甲基转换为酮基的酶活性的多肽的核苷酸序列的DNA链。 一种DNA链，其具有编码具有用于向4-酮-β-雄酮环的3-碳中添加羟基的酶活性的多肽的核苷酸序列。 通过将DNA链引入适当的微生物如大肠杆菌等可以产生各种叶黄素如角黄素，虾青素等。
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