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公开(公告)号：US20230383245A1
公开(公告)日：2023-11-30
申请号：US18446136
申请日：2023-08-08
发明人： Hisato HAYASHI , Misayo OTANI , Koichiro SARUHASHI , Taito NISHINO , Takehisa IWAMA , Tatsuro KANAKI , Ayako AIHARA
IPC分类号： C12N5/00
CPC分类号： C12N5/0068 , C12N2533/70 , C12N2535/00 , C12N2533/72 , C12N2533/78
摘要： The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues, without substantially increasing the viscosity of the solution, by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.
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公开(公告)号：US20200040304A1
公开(公告)日：2020-02-06
申请号：US16499594
申请日：2018-03-30
IPC分类号： C12N5/0775
摘要： The present invention provides a cell carrier containing a nanofiber composed of water-insoluble polysaccharides, preferably chitin or chitosan nanofiber; a carrier capable of being a common carrier in various operations such as i) suspension culture, ii) differentiation induction, iii) transportation and preservation under non-freezing conditions, iv) transplantation, v) recovery of bioactive substance from culture supernatant and the like of adherent cells; and continuous performance of plural operations selected from i) suspension culture, ii) differentiation induction, iii) transportation and preservation under non-freezing conditions, iv) transplantation, and v) recovery of bioactive substance from culture supernatant of adherent cells by using the carrier.
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公开(公告)号：US20210340491A1
公开(公告)日：2021-11-04
申请号：US17272204
申请日：2019-08-30
IPC分类号： C12N5/00
摘要： The present invention provide a medium composition for suspension culture of an adherent cell, containing (1) a chitin nanofiber; and (2) a chitosan nanofiber or a polysaccharide.
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公开(公告)号：US20220364050A1
公开(公告)日：2022-11-17
申请号：US17624744
申请日：2020-07-03
发明人： Daisuke HATANAKA , Tatsuro KANAKI , Hisato HAYASHI
IPC分类号： C12N5/00 , C12N5/0775
摘要： A method for producing a medium composition for suspension culture of an adherent cell, including the following steps: (i) a step of making an extracellular matrix carried on a nanofiber composed of water-insoluble polysaccharides, (ii) a step of adding the extracellular matrix-carrying nanofiber obtained in step (i) to a medium is provided by the present invention.
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公开(公告)号：US20210188894A1
公开(公告)日：2021-06-24
申请号：US16077969
申请日：2017-02-17
发明人： Kohji SEIO , Yoshiaki MASAKI , Keishi YAMAMOTO , Keita YOSHIDA , Yusuke IRIYAMA , Hiroyuki NAKAJIMA , Tatsuro KANAKI
IPC分类号： C07H21/02 , C07H19/067
摘要： Provided is an artificial nucleoside or artificial nucleotide capable of composing an artificial oligonucleotide having superior nuclease resistance. The artificial nucleoside or artificial nucleotide is a compound represented by formula (I) or a salt thereof (wherein, Bx represents a pyrimidine base or purine base, R1, R2, R3 and R4 represent hydrogen atoms, C1-6 alkyl groups or the like, Y represents NR5R6 (wherein, R5 and R6, independently of each other, represent a hydrogen atom, C1-6 alkyl group or the like, or R5 and R6, together with a nitrogen atom bound thereto, form a 3- to 11-membered nitrogen-containing non-aromatic heterocyclic group) or an optionally substituted C2-9 aromatic heterocyclic group, Z1 and Z2 represent hydrogen atoms, hydroxyl group-protecting groups, phosphorous-containing groups or the like, and n represents an integer of 1 to 3).
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公开(公告)号：US20200224196A1
公开(公告)日：2020-07-16
申请号：US16633532
申请日：2018-07-26
IPC分类号： C12N15/113 , A61K31/712 , C12N15/10
摘要： The invention provides a single-stranded oligonucleotide represented by the formula [Xz-Lx]m-X-Y-[Ly-Yz]n, wherein X is represented by Xa-Xb, Xa is coupled with Y, and Xb and Y hybridize. Xa is composed of 1 to 40 nucleotides and contains at least one modified-nucleotide. Xb is composed of 4 to 40 nucleotides and contains at least one modified-nucleotide. Y is composed of 4 to 40 nucleotides and contains at least one ribonucleotide. Xz and Yz are composed of 5 to 40 nucleotides and contain at least one modified-nucleotide. Nucleotide sequences X, Xz and Yz have an antisense sequence capable of hybridizing with a target RNA. Lx and Ly are composed of 0 to 20 nucleotides.
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公开(公告)号：US20200095542A1
公开(公告)日：2020-03-26
申请号：US16662944
申请日：2019-10-24
发明人： Hisato HAYASHI , Misayo OTANI , Koichiro SARUHASHI , Taito NISHINO , Takehisa IWAMA , Tatsuro KANAKI , Ayako AIHARA
IPC分类号： C12N5/00
摘要： The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues without substantially increasing the viscosity of the solution by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.
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公开(公告)号：US20240228935A9
公开(公告)日：2024-07-11
申请号：US18517525
申请日：2023-11-22
摘要： A separation device for separating a solid separation target contained in a suspension obtained by suspension culture of adherent cells by using a fine scaffold material, and a separation method. The separation device has a separation chamber having a first chamber and a second chamber, and the first chamber and the second chamber are divided by a mesh structure for separation. The mesh structure for separation has mesh-holes with a predetermined size to suppress passage of the separation target, and a mesh structure for separation is configured in the separation chamber such that the liquid in the aforementioned suspension has a vertically upward directional component in the advancing direction of the liquid when the liquid passes through the mesh-holes.
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公开(公告)号：US20240132827A1
公开(公告)日：2024-04-25
申请号：US18517525
申请日：2023-11-22
摘要： A separation device for separating a solid separation target contained in a suspension obtained by suspension culture of adherent cells by using a fine scaffold material, and a separation method. The separation device has a separation chamber having a first chamber and a second chamber, and the first chamber and the second chamber are divided by a mesh structure for separation. The mesh structure for separation has mesh-holes with a predetermined size to suppress passage of the separation target, and a mesh structure for separation is configured in the separation chamber such that the liquid in the aforementioned suspension has a vertically upward directional component in the advancing direction of the liquid when the liquid passes through the mesh-holes.
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公开(公告)号：US20230203498A1
公开(公告)日：2023-06-29
申请号：US18054660
申请日：2022-11-11
IPC分类号： C12N15/113 , A61K48/00 , A61K31/7088 , C12P19/34
CPC分类号： C12N15/1137 , C12N15/113 , A61K48/00 , A61K31/7088 , C12P19/34 , C12N2310/11 , C12N2310/315 , C12N2310/32 , C12N2310/351 , C12N2310/3513 , C12N2310/3515
摘要： Provided is a single-stranded oligonucleotide that is capable of controlling a target gene with high efficiency and can be easily produced. The single-stranded oligonucleotide is represented by the formula X-L-Y wherein X and Y hybridize by a first nucleotide sequence portion and a second nucleotide sequence portion. X is composed of 7 to 100 nucleotides, contains at least one modified nucleotide, and has a first nucleotide sequence that is capable of hybridizing with a second oligonucleotide and contains at least four contiguous nucleotides recognized by RNase H. Y is composed of 4 to 100 nucleotides, and has a second nucleotide sequence that is capable of hybridizing with a second oligonucleotide and contains at least one ribonucleotide. At least one of nucleotide sequence X and nucleotide sequence Y has an antisense sequence capable of hybridizing with a target RNA. L is a group derived from a third oligonucleotide that is degraded under physiological conditions.
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