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    Method of measuring homocysteine
    1.
    发明授权
    Method of measuring homocysteine 失效
    测量同型半胱氨酸的方法

    公开(公告)号：US07135306B2

    公开(公告)日：2006-11-14

    申请号：US10546089

    申请日：2004-01-28

    申请人： Nobuyoshi Esaki ,  Tohru Yoshimura ,  Naoto Matsuyama ,  Koji Mizuno ,  Takayuki Bogaki ,  Toshitaka Minetoki ,  Kenji Ozeki

    发明人： Nobuyoshi Esaki ,  Tohru Yoshimura ,  Naoto Matsuyama ,  Koji Mizuno ,  Takayuki Bogaki ,  Toshitaka Minetoki ,  Kenji Ozeki

    IPC分类号： C12Q1/34 ,  C12Q1/37 ,  G01N33/53

    CPC分类号： C12Q1/26 ,  C12N9/1007 ,  C12Q2326/00 ,  G01N33/6815

    摘要： A method for detecting or measuring homocysteine in a sample includes the steps of (a) reacting a D-amino acid present in a sample with a D-amino acid converting enzyme to convert the D-amino acid into a substance that does not serve as a substrate of D-amino acid oxidase or D-amino acid acetyltransferase; (b) reducing homocysteine in the sample with a thiol compound; (c) reacting the reduced homocysteine with a methyltransferase and a methyl donor to newly produce D-amino acid; and (d) reacting the produced D-amino acid with the D-amino acid oxidase or the D-amino acid acetyltransferase in the presence of an SH reagent to produce hydrogen peroxide, and color-developing the produced hydrogen peroxide by using an oxidative color-developing agent.

    摘要翻译： 用于检测或测量样品中同型半胱氨酸的方法包括以下步骤：（a）将样品中存在的D-氨基酸与D-氨基酸转化酶反应，以将D-氨基酸转化为不作为 D-氨基酸氧化酶或D-氨基酸乙酰转移酶的底物; （b）用硫醇化合物还原样品中的高半胱氨酸; （c）使还原的高半胱氨酸与甲基转移酶和甲基供体反应以新产生D-氨基酸; 和（d）在SH试剂的存在下使所产生的D-氨基酸与D-氨基酸氧化酶或D-氨基酸乙酰转移酶反应以产生过氧化氢，并且通过使用氧化颜色显色所产生的过氧化氢 发展代理

    D-serine dehydratase and use thereof
    3.
    发明授权
    D-serine dehydratase and use thereof 有权
    D-丝氨酸脱水酶及其用途

    公开(公告)号：US08367368B2

    公开(公告)日：2013-02-05

    申请号：US13430583

    申请日：2012-03-26

    申请人： Tohru Yoshimura ,  Tomokazu Ito

    发明人： Tohru Yoshimura ,  Tomokazu Ito

    IPC分类号： C12Q1/26 ,  C12N9/00 ,  C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12Q1/527 ,  C12N9/88 ,  G01N2333/988

    摘要： A novel D-serine quantification method that can overcome various disadvantages of a conventional D-serine quantification method; a novel enzyme that can be used in the D-serine quantification method; a gene encoding the enzyme; and the like. Specifically, a novel D-serine dehydratase including (a) a protein having an amino acid sequence set forth in SEQ ID NO: 1 or (b) a protein having an amino acid sequence homologous to the amino acid sequence set forth in SEQ ID NO: 1 and having a D-serine dehydratase activity; and a D-serine quantification method including the steps of reacting a sample with the enzyme, quantifying ammonia or pyruvic acid produced by the reaction, and calculating the amount of D-serine in the sample based on a value produced by the quantification.

    摘要翻译： 一种可以克服常规D-丝氨酸定量方法的各种缺点的新型D-丝氨酸定量方法; 可用于D-丝氨酸定量方法的新型酶; 编码酶的基因; 等等。 具体而言，是一种新型的D-丝氨酸脱水酶，其包含（a）具有SEQ ID NO：1所示的氨基酸序列的蛋白质或（b）具有与SEQ ID NO：1所示的氨基酸序列同源的氨基酸序列的蛋白质 1，具有D-丝氨酸脱水酶活性; 和D-丝氨酸定量方法，其包括使样品与酶反应，定量由反应产生的氨或丙酮酸，并根据定量产生的值计算样品中D-丝氨酸的量。

    Writing instrument with calculator
    4.
    发明授权
    Writing instrument with calculator 失效
    用计算器书写乐器

    公开(公告)号：US4007364A

    公开(公告)日：1977-02-08

    申请号：US572561

    申请日：1975-04-28

    申请人： Shin Ojima ,  Kazuhiko Ohgami ,  Kazutaka Watanabe ,  Tohru Yoshimura ,  Masaya Katou ,  Nozomu Matsuoka

    发明人： Shin Ojima ,  Kazuhiko Ohgami ,  Kazutaka Watanabe ,  Tohru Yoshimura ,  Masaya Katou ,  Nozomu Matsuoka

    IPC分类号： B43K29/093 ,  G06F15/02 ,  H01H13/84 ,  H01H25/06 ,  G06F3/02

    CPC分类号： H01H13/84 ,  B43K29/093 ,  G06F15/02 ,  H01H2019/143 ,  H01H25/065

    摘要： A writing instrument internally houses a miniature electronic calculator which includes a key input section comprising a single key which is subjected to a rotational or translational movement to select one of a plurality of switches. When the key is depressed against a bias applied thereto while in a position in which it has selected one of the switches, the selected switch is controlled thereby to input an associated information into an arithmetic unit of the calculator. The instrument case or key is marked with indicia which designate the key positions for the convenience of selecting input informations. The key input section comprises an insulating substrate on which an array of a plurality of stationary contacts are formed along the direction of movement of the key, a flexible insulating film disposed in opposing relationship with the stationary contacts, and a movable contact deposited on the side of film which faces the stationary contacts so as to extend in the direction of movement of the key. The depression of the key causes a localized portion of the movable contact to bear against one of the stationary contacts, thus forming a switch.

    摘要翻译： 书写工具内部容纳微型电子计算器，其包括键输入部分，该键输入部分包括经受旋转或平移运动以选择多个开关之一的单个键。 当在其中选择了其中一个开关的位置中按压施加偏压的键时，控制所选择的开关，从而将相关信息输入到计算器的运算单元中。 仪器盒或钥匙上标有指示键位置的标记，以方便选择输入信息。 键输入部包括绝缘基板，多个固定触点的阵列沿着键的移动方向形成在其上，柔性绝缘膜以与固定触点相对的方式设置;以及可移动触点，其沉积在该侧面上 的面向固定触点的膜，以沿键的移动方向延伸。 钥匙的按压导致可动触点的局部部分承受固定触点之一，从而形成开关。

    D-SERINE DEHYDRATASE AND USE THEREOF
    5.
    发明申请
    D-SERINE DEHYDRATASE AND USE THEREOF 有权
    D-丝氨酸脱水酶及其用途

    公开(公告)号：US20120244520A1

    公开(公告)日：2012-09-27

    申请号：US13430583

    申请日：2012-03-26

    申请人： Tohru Yoshimura ,  Tomokazu Ito

    发明人： Tohru Yoshimura ,  Tomokazu Ito

    IPC分类号： C12Q1/00

    CPC分类号： C12Q1/527 ,  C12N9/88 ,  G01N2333/988

    摘要： A novel D-serine quantification method that can overcome various disadvantages of a conventional D-serine quantification method; a novel enzyme that can be used in the D-serine quantification method; a gene encoding the enzyme; and the like. Specifically, a novel D-serine dehydratase including (a) a protein having an amino acid sequence set forth in SEQ ID NO: 1 or (b) a protein having an amino acid sequence homologous to the amino acid sequence set forth in SEQ ID NO: 1 and having a D-serine dehydratase activity; and a D-serine quantification method including the steps of reacting a sample with the enzyme, quantifying ammonia or pyruvic acid produced by the reaction, and calculating the amount of D-serine in the sample based on a value produced by the quantification.

    摘要翻译： 一种可以克服常规D-丝氨酸定量方法的各种缺点的新型D-丝氨酸定量方法; 可用于D-丝氨酸定量方法的新型酶; 编码酶的基因; 等等。 具体而言，是一种新型的D-丝氨酸脱水酶，其包含（a）具有SEQ ID NO：1所示的氨基酸序列的蛋白质或（b）具有与SEQ ID NO：1所示的氨基酸序列同源的氨基酸序列的蛋白质 1，具有D-丝氨酸脱水酶活性; 和D-丝氨酸定量方法，其包括使样品与酶反应，定量由反应产生的氨或丙酮酸，并根据定量产生的值计算样品中D-丝氨酸的量。

    D-SERINE DEHYDRATASE AND USE THEREOF
    6.
    发明申请
    D-SERINE DEHYDRATASE AND USE THEREOF 审中-公开
    D-丝氨酸脱水酶及其用途

    公开(公告)号：US20100190196A1

    公开(公告)日：2010-07-29

    申请号：US12445642

    申请日：2007-10-16

    申请人： Tohru Yoshimura ,  Tomokazu Ito

    发明人： Tohru Yoshimura ,  Tomokazu Ito

    IPC分类号： C12Q1/26 ,  C12N9/02 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12P21/06

    CPC分类号： C12Q1/527 ,  C12N9/88 ,  G01N2333/988

    摘要： A novel D-serine quantification method that can overcome various disadvantages of a conventional D-serine quantification method; a novel enzyme that can be used in the D-serine quantification method; a gene encoding the enzyme; and the like. Specifically, a novel D-serine dehydratase including (a) a protein having an amino acid sequence set forth in SEQ ID NO: 1 or (b) a protein having an amino acid sequence homologous to the amino acid sequence set forth in SEQ ID NO: 1 and having a D-serine dehydratase activity; and a D-serine quantification method including the steps of reacting a sample with the enzyme, quantifying ammonia or pyruvic acid produced by the reaction, and calculating the amount of D-serine in the sample based on a value produced by the quantification.

    摘要翻译： 一种可以克服常规D-丝氨酸定量方法的各种缺点的新型D-丝氨酸定量方法; 可用于D-丝氨酸定量方法的新型酶; 编码酶的基因; 等等。 具体而言，是一种新型的D-丝氨酸脱水酶，其包含（a）具有SEQ ID NO：1所示的氨基酸序列的蛋白质或（b）具有与SEQ ID NO：1所示的氨基酸序列同源的氨基酸序列的蛋白质 1，具有D-丝氨酸脱水酶活性; 和D-丝氨酸定量方法，其包括使样品与酶反应，定量由反应产生的氨或丙酮酸，并根据定量产生的值计算样品中D-丝氨酸的量。