公开(公告)号：US20240307869A1

公开(公告)日：2024-09-19

申请号：US18576158

申请日：2022-06-21

Applicant: OPTOLANE TECHNOLOGIES INC.

Inventor： Seung Sun CHO ,  An Shik CHOI ,  Byoung Joo KWAK ,  Sergiy OLEKSANDROV

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L2200/0642 ,  B01L2300/044 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/161 ,  B01L2400/0406

Abstract: Disclosed is a PCR module in which a sample can be injected using the capillary effect. The PCR module comprises: a microfluidic chamber which includes an inlet part for inputting a sample and is manufactured by injection molding; a well array which is disposed on the lower surface of the microfluidic chamber and includes a plurality of micro-wells of which the upper and lower portions are perforated; and a capillary member providing a path so that the sample input through the inlet part reaches the micro-wells through the capillary effect. When moving a PCR solution to a reaction space using the capillary effect and filling the reaction space with the solution, it is possible to prevent air pockets from forming in the corners or edge regions of the well array which is the reaction space. Accordingly, it is possible to prevent errors caused by air pockets from occurring in PCR test results. In addition, since the well array is located on a CMOS photosensor array, PCR reactions can be measured in real time.

公开(公告)号：US20240286126A1

公开(公告)日：2024-08-29

申请号：US18573475

申请日：2022-06-10

Applicant: OPTOLANE TECHNOLOGIES INC.

Inventor： Seung Sun CHO ,  An Shik CHOI ,  Byoung Joo KWAK ,  Sergiy OLEKSANDROV ,  Jae Hun KIM

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L2200/0684 ,  B01L2200/14 ,  B01L2300/042 ,  B01L2300/0829 ,  B01L2300/161 ,  B01L2400/088

Abstract: Disclosed is an in vitro diagnostic chip having multiple levels of hydrophilicity, which differentially controls the flow rates of a liquid sample and thus enables the liquid sample to be evenly distributed all the way to corner or edge regions of a reaction space. The in vitro diagnostic chip having multiple levels of hydrophilicity comprises: a well array comprising a plurality of wells; and a multi-hydrophilic coating layer coated on the well array and controlling the flow rates of a sample so as to be mutually different. The hydrophilic coating layer is formed on the well array so as to have high hydrophilicity in accordance with the center region of the well array, and to have low hydrophilicity in accordance with the peripheral region of the well array, and thus the formation of an air pocket in the reaction space may be blocked. Accordingly, the occurrence of an error caused by the air pocket in a PCR test result may be prevented.

公开(公告)号：US20190329254A1

公开(公告)日：2019-10-31

申请号：US16390380

申请日：2019-04-22

Applicant: OPTOLANE Technologies Inc.

Inventor： Sergiy OLEKSANDROV ,  Do Young LEE ,  Min Sik SONG ,  Kyung Hak CHOI

IPC: B01L3/00 ,  C12Q1/6806 ,  C12Q1/686

Abstract: A cartridge for digital real-time Polymerase chain reaction (PCR) includes a microfluidic chamber, a well array, a CMOS photo sensor array and a PCB. The microfluidic chamber includes an inlet formed for injection of a liquid sample, the microfluidic chamber being capable of injection molding. The well array includes a plurality of microwells through which upper and lower portions are perforated and being attached to a lower surface of the microfluidic chamber. The CMOS photo sensor array is disposed below the well array to capture a response image of a sample filled in microwells of the well array. The PCB has a vent formed for vacuum processing of micro flow path formed in the microfluidic chamber, a space formed between the well array and the microfluidic chamber, and a microwell formed in the well array as the liquid sample is injected through the inlet.