公开(公告)号：US20160047749A1

公开(公告)日：2016-02-18

申请号：US14824764

申请日：2015-08-12

Applicant: OPTOLANE TECHNOLOGIES INC.

Inventor： Do Young LEE ,  In Gyun Jeon

IPC: G01N21/64 ,  H01L31/18 ,  H01L31/12

CPC classification number: G01N21/6428 ,  G01N21/6454 ,  G01N2201/062 ,  H01L31/125 ,  H01L31/18

Abstract: The present disclosure a biochip including a side emitting-type light-emitting device, in which the bio-chip includes: a light-emitting device for emitting light from a fluorescent material; reflective layers provided over and under the light-emitting device so as to emit light from the sides of the light-emitting device; and reaction regions formed by etching of flanking regions of the light-emitting device. In the biochip, light emitted from the sides of the light-emitting device causes a biochemical reaction in the reaction regions. According to the present disclosure, light emitted from the light-emitting device moves only laterally without being transferred to the top or bottom of the bio-layer, and is transferred to the reaction regions formed by etching of flanking regions of the light-emitting device, so that a biochemical reaction in the reaction regions can be more efficiently performed.

Abstract translation: 本发明公开了一种生物芯片，其包括侧面发射型发光器件，其中所述生物芯片包括：用于从荧光材料发射光的发光器件; 设置在发光装置的上下的反射层，以从发光装置的侧面发光; 以及通过蚀刻发光器件的侧面区域形成的反应区域。 在生物芯片中，从发光装置的侧面发射的光在反应区域引起生化反应。 根据本公开，从发光装置发射的光仅在横向移动而不转移到生物层的顶部或底部，并且被转移到通过蚀刻发光装置的侧面区域形成的反应区域 ，从而可以更有效地进行反应区域的生化反应。

公开(公告)号：US20240307869A1

公开(公告)日：2024-09-19

申请号：US18576158

申请日：2022-06-21

Applicant: OPTOLANE TECHNOLOGIES INC.

Inventor： Seung Sun CHO ,  An Shik CHOI ,  Byoung Joo KWAK ,  Sergiy OLEKSANDROV

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L2200/0642 ,  B01L2300/044 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/161 ,  B01L2400/0406

Abstract: Disclosed is a PCR module in which a sample can be injected using the capillary effect. The PCR module comprises: a microfluidic chamber which includes an inlet part for inputting a sample and is manufactured by injection molding; a well array which is disposed on the lower surface of the microfluidic chamber and includes a plurality of micro-wells of which the upper and lower portions are perforated; and a capillary member providing a path so that the sample input through the inlet part reaches the micro-wells through the capillary effect. When moving a PCR solution to a reaction space using the capillary effect and filling the reaction space with the solution, it is possible to prevent air pockets from forming in the corners or edge regions of the well array which is the reaction space. Accordingly, it is possible to prevent errors caused by air pockets from occurring in PCR test results. In addition, since the well array is located on a CMOS photosensor array, PCR reactions can be measured in real time.

公开(公告)号：US20160271611A1

公开(公告)日：2016-09-22

申请号：US15064816

申请日：2016-03-09

Applicant: OPTOLANE Technologies Inc.

Inventor： DoYoung LEE ,  In Gyun JEON ,  An Shik CHOI ,  Kyung Hak CHOI

IPC: B01L7/00 ,  C12Q1/68

CPC classification number: B01L7/52 ,  B01L3/50851 ,  B01L2200/028 ,  B01L2200/147 ,  B01L2300/0663 ,  B01L2300/0681 ,  B01L2300/0819 ,  B01L2300/18 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1844 ,  B01L2300/185 ,  C12Q1/686 ,  G01N21/64

Abstract: A polymerase chain reaction (PCR) module is detachably combined with a reader system. The reader system includes a central processing unit (CPU) receiving a photo sensing signal to calculate gene amplification amount in real time and generating a temperature control signal based on a temperature signal and a temperature control information. The PCR module includes a photo sensor assembly, a partition wall, and an interface module. The photo sensor assembly includes a plurality of photo sensors and a temperature sensor. The photo sensors are arranged in an array shape to sense emission light generated from a specimen to generate the photo sensing signal. The partition wall is protruded from the photo sensor assembly to define a reaction space in which the specimen is received. The interface module is electrically connected to the photo sensor assembly to transmit the photo sensing signal and the temperature signal to the reader system.

Abstract translation: 聚合酶链反应（PCR）模块可与读取器系统可拆卸地组合。 读取器系统包括中央处理单元（CPU），其接收感光信号以实时计算基因放大量，并且基于温度信号和温度控制信息产生温度控制信号。 PCR模块包括光传感器组件，分隔壁和接口模块。 光传感器组件包括多个光传感器和温度传感器。 光传感器被布置成阵列形状以检测从样本产生的发射光以产生光感测信号。 分隔壁从光传感器组件突出以限定其中接收样品的反应空间。 接口模块电连接到光传感器组件，以将感光信号和温度信号传输到读取器系统。

公开(公告)号：US20160152941A1

公开(公告)日：2016-06-02

申请号：US14889278

申请日：2013-05-06

Applicant: Optolane Technologies Inc.

Inventor： Songyi KIM

IPC: C12M1/34 ,  C12M1/32 ,  C12M1/42 ,  C12M1/00 ,  C12Q1/02 ,  C12M1/36

CPC classification number: C12M41/46 ,  C12M23/12 ,  C12M23/50 ,  C12M27/16 ,  C12M35/04 ,  C12M41/26 ,  C12M41/36 ,  C12M41/48 ,  C12Q1/02 ,  G01N15/1468

Abstract: The present invention relates to a system for analyzing cells and monitoring cell culture and a method for analyzing cells and monitoring cell culture using the same. The system comprises M×N matrix camera modules disposed below the cell culture unit in which a cell culture vessel is received, and thus can simultaneously analyze changes in the cells being cultured in a multi-well cell culture vessel such as a 96-well plate. Particularly, the system enables a desired portion to be observed by selectively switching on camera modules corresponding to the desired portion. In addition, the system can microscopically observe entire wells or can microscopically observe the region of interest of a single cell at high magnifications, can display dynamic cell images in real time, can overcome the problem of contamination during cell analysis and culture, and can remotely check the state of culture in real time.

Abstract translation: 本发明涉及一种用于分析细胞和监测细胞培养的系统，以及用于分析细胞并监测细胞培养的方法。 该系统包括设置在细胞培养单元下方的M×N矩阵相机模块，其中容纳细胞培养容器，因此可以同时分析在诸如96孔板的多孔细胞培养容器中培养的细胞的变化 。 特别地，该系统能够通过选择性地打开与所需部分对应的相机模块来观察期望的部分。 此外，该系统可以显微镜观察整个井，或者可以以高倍数微观观察单个细胞的感兴趣区域，可以实时显示动态细胞图像，可以克服细胞分析和培养过程中的污染问题，并可远程 实时检查文化状况。

公开(公告)号：US10710084B2

公开(公告)日：2020-07-14

申请号：US16361594

申请日：2019-03-22

Applicant: OPTOLANE Technologies Inc.

Inventor： DoYoung Lee ,  In Gyun Jeon ,  An Shik Choi ,  Kyung Hak Choi

IPC: C12P19/34 ,  B01L7/00 ,  B01L3/00 ,  G01N21/64 ,  C12Q1/686

Abstract: A polymerase chain reaction (PCR) module is detachably combined with a reader system. The reader system includes a central processing unit (CPU) receiving a photo sensing signal to calculate gene amplification amount in real time and generating a temperature control signal based on a temperature signal and a temperature control information. The PCR module includes a photo sensor assembly, a partition wall, and an interface module. The photo sensor assembly includes a plurality of photo sensors and a temperature sensor. The photo sensors are arranged in an array shape to sense emission light generated from a specimen to generate the photo sensing signal. The partition wall is protruded from the photo sensor assembly to define a reaction space in which the specimen is received. The interface module is electrically connected to the photo sensor assembly to transmit the photo sensing signal and the temperature signal to the reader system.

公开(公告)号：US10705021B2

公开(公告)日：2020-07-07

申请号：US15855013

申请日：2017-12-27

Applicant: OPTOLANE TECHNOLOGIES INC.

Inventor： Do Young Lee

IPC: G01N21/64 ,  H01L27/146 ,  C12Q1/6837 ,  H01L27/14 ,  G01N33/53 ,  B01L3/00

Abstract: Provided is a biochip having improved fluorescent signal sensing properties. A filter layer is provided between a bio-layer and a light sensor layer so as to remove noise generated by stray light during a bio-reaction process. Thereby, the sensitivity of the light sensor layer can be enhanced.

公开(公告)号：US20180238872A1

公开(公告)日：2018-08-23

申请号：US15881986

申请日：2018-01-29

Applicant: OPTOLANE Technologies Inc.

Inventor： Do Young Lee ,  Kyung Hak Choi

IPC: G01N33/543 ,  G01N21/76 ,  B01L3/00

CPC classification number: G01N33/54393 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/027 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2400/0481 ,  B01L2400/06 ,  G01N21/645 ,  G01N21/6454 ,  G01N21/76 ,  G01N33/54366 ,  G01N2021/0325 ,  G01N2021/6471 ,  G01N2021/6482 ,  G02B5/223

Abstract: An immunoassay cartridge is disclosed that can enhance the reliability of an antigen-antibody reaction while increasing a speed of an antigen-antibody reaction. An immunoassay cartridge includes a reaction chamber and a fluorescence sensor assembly. A plurality of antibodies or antigens is attached to an inner surface including a bottom surface of the reaction chamber closest to the sensor. The fluorescence sensor assembly is disposed on a bottom surface of the reaction chamber. Since the bottom surface of the reaction chamber and the upper surface of the fluorescence sensor assembly are arranged to coincide with each other, even if fluid is repeatedly moved in a first direction after the fluid moves in a second direction in the reaction chamber and then moved in the first direction, there is no obstacle in the movement of the fluid. Thus, it is possible to increase the probability of antigen-antibody reaction in the reaction chamber.

公开(公告)号：US10018562B2

公开(公告)日：2018-07-10

申请号：US14824764

申请日：2015-08-12

Applicant: OPTOLANE TECHNOLOGIES INC.

Inventor： Do Young Lee ,  In Gyun Jeon

IPC: G01N21/64 ,  H01L31/18 ,  H01L31/12

CPC classification number: G01N21/6428 ,  G01N21/6452 ,  G01N21/6454 ,  G01N21/6456 ,  G01N2201/062 ,  H01L31/125 ,  H01L31/18

Abstract: The present disclosure a biochip including a side emitting-type light-emitting device, in which the bio-chip includes: a light-emitting device for emitting light from a fluorescent material; reflective layers provided over and under the light-emitting device so as to emit light from the sides of the light-emitting device; and reaction regions formed by etching of flanking regions of the light-emitting device. In the biochip, light emitted from the sides of the light-emitting device causes a biochemical reaction in the reaction regions. According to the present disclosure, light emitted from the light-emitting device moves only laterally without being transferred to the top or bottom of the bio-layer, and is transferred to the reaction regions formed by etching of flanking regions of the light-emitting device, so that a biochemical reaction in the reaction regions can be more efficiently performed.

公开(公告)号：US20180156731A1

公开(公告)日：2018-06-07

申请号：US15831837

申请日：2017-12-05

Applicant: OPTOLANE Technologies Inc.

Inventor： Jun Ho Won ,  Do Young Lee ,  Kyung Hak Choi ,  An Shik Choi

IPC: G01N21/64 ,  C12Q1/686 ,  G01N21/21 ,  B01L3/00

CPC classification number: G01N21/6428 ,  B01L3/50851 ,  C12Q1/686 ,  G01J1/0252 ,  G01J1/0488 ,  G01J3/0286 ,  G01J3/4406 ,  G01J3/443 ,  G01J3/51 ,  G01J2003/468 ,  G01N21/21 ,  G01N2021/6471 ,  G02B5/223

Abstract: The wide-angle emission filter includes a base matrix, a photoresist, and a colorant. The base matrix has a flat shape and including a transparent material. The base matrix does not generate fluorescent light or phosphorescent light by an excitation light. The photoresist is disposed in the base matrix. The photoresist is fixed in a solid state through at least one method selected from the group consisting of thermal hardening, photo hardening, and drying. The colorant is disposed in the base matrix and includes light having a predetermined wavelength range. The wide-angle emission filter filters the excitation light regardless of an incident angle of the excitation light.

公开(公告)号：US09885658B2

公开(公告)日：2018-02-06

申请号：US14863511

申请日：2015-09-24

Applicant: OPTOLANE TECHNOLOGIES INC.

Inventor： Do Young Lee

IPC: G01N21/64 ,  H01L27/14 ,  H01L27/146 ,  G01N33/53 ,  B01L3/00

CPC classification number: G01N21/6428 ,  B01L3/5027 ,  G01N21/6454 ,  G01N21/6456 ,  G01N21/6458 ,  G01N21/6486 ,  G01N33/5304 ,  G01N2201/062 ,  H01L27/14 ,  H01L27/14621 ,  H01L27/14685 ,  H01L27/14687

Abstract: A method for manufacturing a biochip having improved fluorescent signal sensing properties, and a biochip manufactured by the manufacturing method. A filter layer is provided between a bio-layer and a light sensor layer so as to remove noise generated by stray light during a bio-reaction process. Thereby, the sensitivity of the light sensor layer can be enhanced.