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1.发明授权公开(公告)号：US07601493B2
公开(公告)日：2009-10-13
申请号：US10627950
申请日：2003-07-24
申请人： Ray R. Radtkey , Lance C. Held , Robert B. Wallace , Karen Menge , David Canter
发明人： Ray R. Radtkey , Lance C. Held , Robert B. Wallace , Karen Menge , David Canter
CPC分类号： C12Q1/6827 , C12Q1/6837 , Y10T436/143333 , C12Q2537/149 , C12Q2525/161 , C12Q2535/131 , C12Q2565/518 , C12Q2525/186
摘要： An assay system and methods are described where patient samples containing genomic DNA are analyzed for the presence of known genetic polymorphisms using a universal reporter strategy. In a preferred embodiment, the amplified DNA is localized at test sites in an array of sites on a microchip followed by a series of hybridization reactions that screen for the presence of a single mutation from among a number of mutations, and allow the identification of specific mutations. In addition to universal reporters, the assay may use blockers and discriminators for screening and identification of known polymorphisms.
摘要翻译： 描述了使用通用记录策略分析含有基因组DNA的患者样品存在已知遗传多态性的测定系统和方法。 在优选的实施方案中，扩增的DNA定位在微芯片上位点阵列中的测试位点处，随后进行一系列杂交反应，筛选多个突变中单个突变的存在，并允许鉴定特异性 突变 除了通用记者之外，该测定可以使用阻断剂和鉴定剂来筛选和鉴定已知的多态性。
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2.发明授权公开(公告)号：US08772467B2
公开(公告)日：2014-07-08
申请号：US12574875
申请日：2009-10-07
申请人： Ray R. Radtkey , Lance C. Held , Robert B. Wallace , Karen Menge , David Canter
发明人： Ray R. Radtkey , Lance C. Held , Robert B. Wallace , Karen Menge , David Canter
CPC分类号： C12Q1/6827 , C12Q1/6837 , Y10T436/143333 , C12Q2537/149 , C12Q2525/161 , C12Q2535/131 , C12Q2565/518 , C12Q2525/186
摘要： An assay system and methods are described where patient samples containing genomic DNA are analyzed for the presence of known genetic polymorphisms using a universal reporter strategy. In a preferred embodiment, the amplified DNA is localized at test sites in an array of sites on a microchip followed by a series of hybridization reactions that screen for the presence of a single mutation from among a number of mutations, and allow the identification of specific mutations. In addition to universal reporters, the assay may use blockers and discriminators for screening and identification of known polymorphisms.
摘要翻译： 描述了使用通用记录策略分析含有基因组DNA的患者样品存在已知遗传多态性的测定系统和方法。 在优选的实施方案中，扩增的DNA定位在微芯片上位点阵列中的测试位点处，随后进行一系列杂交反应，筛选多个突变中单个突变的存在，并允许鉴定特异性 突变 除了通用记者之外，该测定可以使用阻断剂和鉴定剂来筛选和鉴定已知的多态性。
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3.发明申请公开(公告)号：US20100167960A1
公开(公告)日：2010-07-01
申请号：US12574875
申请日：2009-10-07
申请人： Ray R. Radtkey , Lance C. Held , Robert B. Wallace , Karen Menge , David Canter
发明人： Ray R. Radtkey , Lance C. Held , Robert B. Wallace , Karen Menge , David Canter
IPC分类号： C40B60/12
CPC分类号： C12Q1/6827 , C12Q1/6837 , Y10T436/143333 , C12Q2537/149 , C12Q2525/161 , C12Q2535/131 , C12Q2565/518 , C12Q2525/186
摘要： An assay system and methods are described where patient samples containing genomic DNA are analyzed for the presence of known genetic polymorphisms using a universal reporter strategy. In a preferred embodiment, the amplified DNA is localized at test sites in an array of sites on a microchip followed by a series of hybridization reactions that screen for the presence of a single mutation from among a number of mutations, and allow the identification of specific mutations. In addition to universal reporters, the assay may use blockers and discriminators for screening and identification of known polymorphisms.
摘要翻译： 描述了使用通用记录策略分析含有基因组DNA的患者样品存在已知遗传多态性的测定系统和方法。 在优选的实施方案中，扩增的DNA定位在微芯片上位点阵列中的测试位点处，随后进行一系列杂交反应，筛选多个突变中单个突变的存在，并允许鉴定特异性 突变 除了通用记者之外，该测定可以使用阻断剂和鉴定剂来筛选和鉴定已知的多态性。
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4.发明申请公开(公告)号：US20060065531A1
公开(公告)日：2006-03-30
申请号：US11234054
申请日：2005-09-22
申请人： Daniel Smolko , Paul Swanson , Dalibor Hodko , David Canter , Robert Haigis , Tricia Patterson
发明人： Daniel Smolko , Paul Swanson , Dalibor Hodko , David Canter , Robert Haigis , Tricia Patterson
IPC分类号： C07K1/26 , G01N27/447
CPC分类号： C12Q1/6825 , C12Q2565/607
摘要： Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electrophoretic transportation of the nucleic acid towards the location. The nucleic acid is then hybridized to a nucleic probed located at the location. The reducing agent in the low conductivity buffer may also be acting as a chaotropic agent.
摘要翻译： 通过向装置提供具有还原剂的低电导率缓冲液来将核酸在电极装置上的运输和杂交的方法。 低电导率缓冲液也可以含有两性离子。 将电流和电压施加到装置的位置以实现核酸向该位置的电泳运输。 然后将核酸与位于该位置的核酸探针杂交。 低电导率缓冲液中的还原剂也可用作离液剂。
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5.发明授权公开(公告)号：US07314542B2
公开(公告)日：2008-01-01
申请号：US11234054
申请日：2005-09-22
申请人： Daniel D. Smolko , Paul D. Swanson , Dalibor Hodko , David Canter , Robert W. Haigis , Tricia Patterson
发明人： Daniel D. Smolko , Paul D. Swanson , Dalibor Hodko , David Canter , Robert W. Haigis , Tricia Patterson
IPC分类号： G01N27/447
CPC分类号： C12Q1/6825 , C12Q2565/607
摘要： Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electrophoretic transportation of the nucleic acid towards the location. The nucleic acid is then hybridized to a nucleic probed located at the location. The reducing agent in the low conductivity buffer may also be acting as a chaotropic agent.
摘要翻译： 通过向装置提供具有还原剂的低电导率缓冲液来将核酸在电极装置上的运输和杂交的方法。 低电导率缓冲液也可以含有两性离子。 将电流和电压施加到装置的位置以实现核酸向该位置的电泳运输。 然后将核酸与位于该位置的核酸探针杂交。 低电导率缓冲液中的还原剂也可用作离液剂。
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