公开(公告)号：US11724246B2

公开(公告)日：2023-08-15

申请号：US16599260

申请日：2019-10-11

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Martin Eduardo Silvestre ,  Stephan Hug

IPC: B01J20/26 ,  B01J20/28 ,  B01J20/32 ,  B03C1/01 ,  G01N33/543

CPC classification number: B01J20/28009 ,  B01J20/267 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/3234 ,  B01J20/3272 ,  B01J20/3282 ,  B01J20/3293 ,  B03C1/01 ,  G01N33/54326 ,  B03C2201/18 ,  B03C2201/26

Abstract: A method of preparing a magnetic particle having a polymer matrix (P) and at least one magnetic core (M), preferably at least two magnetic cores (M), wherein the polymer matrix (P) comprises at least one hypercrosslinked polymer, wherein the method comprises (i) providing at least one magnetic core (M), preferably at least two magnetic cores (M), (ii) providing polymer precursor molecules, (iii) polymerizing the polymer precursor molecules according to (ii) in the presence of the at least one magnetic core (M), thereby forming a particle comprising the at least one magnetic core (M) is disclosed. Further, particles obtained or obtainable by this method as well as to the use of these particles are disclosed. In a further aspect, a method for determining at least one analyte in a fluid sample having the step of contacting of the magnetic particle with a fluid sample having or suspected of having the at least one analyte is disclosed.

公开(公告)号：US20200041502A1

公开(公告)日：2020-02-06

申请号：US16599480

申请日：2019-10-11

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Jens Christian Bolle ,  Martin Eduardo Silvestre ,  Stephan Hug ,  Carolyn Bylda

IPC: G01N33/543 ,  G01N33/553 ,  B01J13/14 ,  B03C1/01

Abstract: The present disclosure relates to magnetic particles, wherein each particle has a polymer matrix and at least one magnetic core (M), wherein the polymer matrix has at least one crosslinked polymer and wherein the magnetic particle has a particle size in the range of from 1 to 60 micrometer. Further, the present disclosure relates to a method of preparing such particles, and to particles obtainable or obtained by said method. Moreover, the present disclosure uses these magnetic particles for qualitative and/or quantitative determination of at least one analyte in a fluid. Further, the present disclosure relates to a method for determining at least one analyte in a fluid sample using the contacting of a magnetic particle of the disclosure or a magnetic particle obtained by the method of the present disclosure with a fluid sample having or suspected to have the at least one analyte.

公开(公告)号：US20250069787A1

公开(公告)日：2025-02-27

申请号：US18713584

申请日：2022-11-23

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Martin Eduardo Silvestre ,  Sabine Eisold ,  Dieter Heindl ,  Kristina Peters

IPC: H01F1/33 ,  H01F1/00 ,  H01F1/34

Abstract: In a first aspect, the invention relates to a method for preparing a magnetic bead comprising at least one magnetic particle (M) and a silica coating, wherein the silica coating comprises at least two silica layers, the method comprising steps (a) to (d), wherein at least one of steps (b), (c) and (d) is/are done under sonication, wherein sonication is done with an amplitude (peak-to-peak) in the range of from 50 to 250 μm. A second aspect of the invention is related to a magnetic bead comprising (i) at least one magnetic particle (M) and (ii) a silica coating, wherein the silica coating comprises at least two silica layers; wherein the magnetic bead is stable against 7.5 M hydrochlorid acid and has a metal (cation) leaching rate in 7.5 M hydrochloric acid in the range of from 0.1 to 10%, wherein the metal (cation) leaching is determined according to a complex formation of Fe2+ with bathophenanthroline according to Reference Example 8.2. In a third aspect, the invention relates to a functionalized magnetic bead comprising at least one, magnetic bead according to the second aspect, wherein an outer silica layer is functionalized with at least one group selected from the group consisting of amino group, azide group, alkyne group, carboxyl group, thiol group, epoxy group, aryl group and alkyl group. A fourth aspect of the invention is directed to a process for functionalizing a magnetic bead according to the second aspect comprising at least one magnetic particle (M) and a silica coating, wherein the silica coating comprises at least two silica layers. A fifth aspect of the invention is related to a method for preparing magnetic Fe3O4 supra particles and a sixth aspect relates to the use of a magnetic bead according to the second aspect or of a functionalized according to the fourth aspect for immobilization of acid stable biocatalysts or for solid-phase organic synthesis using acid-stable linker.

公开(公告)号：US20200038839A1

公开(公告)日：2020-02-06

申请号：US16599260

申请日：2019-10-11

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Martin Eduardo Silvestre ,  Stephan Hug

IPC: B01J20/28 ,  B01J20/26 ,  B01J20/32 ,  B03C1/01 ,  G01N33/543

Abstract: The present disclosure relates to a method of preparing a magnetic particle having a polymer matrix (P) and at least one magnetic core (M), preferably at least two magnetic cores (M), wherein the polymer matrix (P) comprises at least one hypercrosslinked polymer, wherein the method comprises (i) providing at least one magnetic core (M), preferably at least two magnetic cores (M), (ii) providing polymer precursor molecules, (iii) polymerizing the polymer precursor molecules according to (ii) in the presence of the at least one magnetic core (M), thereby forming a particle comprising the at least one magnetic core (M). Further, the present disclosure relates to particles obtained or obtainable by this method as well as to the use of these particles. In a further aspect, the disclosure relates to a method for determining at least one analyte in a fluid sample having the step of contacting of the magnetic particle with a fluid sample having or suspected of having the at least one analyte.

公开(公告)号：US20230381743A1

公开(公告)日：2023-11-30

申请号：US18338633

申请日：2023-06-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Martin Eduardo Silvestre ,  Stephan Hug

IPC: B01J20/28 ,  B01J20/26 ,  B01J20/32 ,  B03C1/01 ,  G01N33/543

CPC classification number: B01J20/28009 ,  B01J20/267 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B03C2201/26 ,  B01J20/3272 ,  B03C1/01 ,  G01N33/54326 ,  B01J20/3282 ,  B01J20/3293 ,  B03C2201/18 ,  B01J20/3234

Abstract: The present disclosure relates to a method of preparing a magnetic particle having a polymer matrix (P) and at least one magnetic core (M), preferably at least two magnetic cores (M), wherein the polymer matrix (P) comprises at least one hypercrosslinked polymer, wherein the method comprises (i) providing at least one magnetic core (M), preferably at least two magnetic cores (M), (ii) providing polymer precursor molecules, (iii) polymerizing the polymer precursor molecules according to (ii) in the presence of the at least one magnetic core (M), thereby forming a particle comprising the at least one magnetic core (M). Further, the present disclosure relates to particles obtained or obtainable by this method as well as to the use of these particles. In a further aspect, the disclosure relates to a method for determining at least one analyte in a fluid sample having the step of contacting of the magnetic particle with a fluid sample having or suspected of having the at least one analyte.