公开(公告)号：US06181414B2

公开(公告)日：2001-01-30

申请号：US09368521

申请日：1999-08-05

申请人： Ryan S. Raz ,  Gordon Rosenblatt ,  Daniel S. Maclean

发明人： Ryan S. Raz ,  Gordon Rosenblatt ,  Daniel S. Maclean

IPC分类号： G01J300

CPC分类号： G01N21/3563 ,  G01N15/1475 ,  G01N21/3151 ,  G01N2021/3133 ,  G01N2021/3137 ,  G01N2021/3148 ,  G01N2021/3155

摘要： A multi-channel spectral imaging module for an automated testing system for cytological applications. The multi-channel spetral imaging module generates four channels of digitized images of a cytological specimen for further processing in the automated testing system. The multi-spectral imaging module comprises a visible light source and an infrared light source, an optical imaging module, a prism module, and an output stage. The cytological specimen is strobed by the visible light and infrared light sources and the illuminated images of the specimen are focussed and coupled to the prism module by the optical imaging module. The prism module breaks the illuminated image into four channels comprising three visible light channels and an infrared channel. The output stage comprises a CCD array sensor for each channel. The CCD array sensor digitizes the image for channel and produces an output for further processing. The digitized output from the infrared channel provides additional information which is used by the automated testing system. The additional information derived from the infrared channel includes segmentation information, new identifying features for the specimen, and discrimination measures independent of the visible light channels. In another embodiment, a fifth channel is provided for a reference infrared output and the two infrared channels are operated in a differential mode.

摘要翻译： 用于细胞学应用的自动化测试系统的多通道光谱成像模块。 多通道spetral成像模块生成四个通道的细胞学标本的数字化图像，用于在自动化测试系统中进一步处理。 多光谱成像模块包括可见光源和红外光源，光学成像模块，棱镜模块和输出级。 细胞学样本被可见光和红外光源选通，并且通过光学成像模块将样本的照射图像聚焦并耦合到棱镜模块。 棱镜模块将照明图像分成四个通道，包括三个可见光通道和一个红外通道。 输出级包括用于每个通道的CCD阵列传感器。 CCD阵列传感器将通道图像数字化，并产生一个输出进一步处理。 红外通道的数字化输出提供了自动测试系统使用的附加信息。 从红外线通道导出的附加信息包括分割信息，样本的新识别特征以及独立于可见光通道的鉴别度量。 在另一个实施例中，为参考红外输出提供第五通道，并且两个红外通道以差分模式操作。

公开(公告)号：US06230154B1

公开(公告)日：2001-05-08

申请号：US09115608

申请日：1998-07-15

申请人： Ryan S. Raz ,  Iouri Lappa

发明人： Ryan S. Raz ,  Iouri Lappa

IPC分类号： G06F1730

CPC分类号： G06F17/30277 ,  G06F19/00 ,  G06F19/324 ,  G16H50/70 ,  Y10S707/99932 ,  Y10S707/99933 ,  Y10S707/99934 ,  Y10S707/99935 ,  Y10S707/99945

摘要： A system and method for database element retrieval. A query comprises identifying which elements in the database are close or similar to an example which is specified in the query. The query is performed by defining a hyper-box that surrounds the example in the database space. Once the hyper-box is defined, the elements contained within the hyper-box will be close or similar to the example in the query. The elements contained in the hyper-box may be subsequently ranked in the database space using a Euclidean concept of distance.

摘要翻译： 一种用于数据库元素检索的系统和方法。 查询包括识别数据库中的哪些元素与查询中指定的示例关联或相似。 通过定义围绕数据库空间中的示例的超级框来执行查询。 一旦定义了超级框，超级框中包含的元素将与查询中的示例相似或相似。 包含在超盒中的元素可以随后使用欧几里德距离概念在数据库空间中排名。

公开(公告)号：US06291234B1

公开(公告)日：2001-09-18

申请号：US09382607

申请日：1999-08-25

申请人： Ryan S. Raz ,  Zhaoyu Wang

发明人： Ryan S. Raz ,  Zhaoyu Wang

IPC分类号： C12M124

CPC分类号： C12M45/22 ,  C12M33/02

摘要： An apparatus and method for transferring exfoliated cells from a variety of specimen collection devices to a preservation fluid contained in a specimen vial. The apparatus comprises an interior frame member which is inserted into the specimen vial. The interior frame member includes a cross-bar member and an open-ended well. The cross-bar member includes a blade which provides an edge for scraping exfoliated cells from a spatula type specimen collection device. The cross-bar member also provides a lower edge for disconnecting a broom sampling head from a broom type collection device of disconnecting a combination type sampling head from a Combi™ type specimen collection device. The open-ended well comprises a sloped interior wall and a lower edge for removing exfoliated cells from a brush sampling head on a brush type collection device.

摘要翻译： 将剥离的细胞从各种样品收集装置转移到包含在样品瓶中的保存液体的装置和方法。 该装置包括插入到样品瓶中的内部框架构件。 内部框架构件包括横杆构件和开放式井。 横杆构件包括叶片，其提供用于从刮刀型样本收集装置刮除脱落细胞的边缘。 横杆构件还提供了一个下边缘，用于将笤帚取样头从与Combi TM型样本采集装置断开组合型取样头的扫帚式收集装置断开连接。 开放式井包括倾斜的内壁和用于从刷式收集装置上的刷取样头去除脱落细胞的下边缘。

公开(公告)号：US06463425B2

公开(公告)日：2002-10-08

申请号：US09040378

申请日：1998-03-18

申请人： Ryan S. Raz

发明人： Ryan S. Raz

IPC分类号： G06N302

CPC分类号： G01N15/1475 ,  G06K9/00127 ,  G06T7/11 ,  G06T7/136 ,  G06T7/155 ,  G06T2207/10056 ,  G06T2207/20036 ,  G06T2207/20084 ,  G06T2207/30024

摘要： A neural network assisted multi-spectral segmentation method and system. According to the invention, three images having different optical bands are acquired for the same micrographic scene of a biological sample. The images are processed and a cellular material map is generated identifying cellular material. The cellular material map is then applied to a neural network. The neural network classifies the cellular material map into nuclear objects and cytoplasmic objects by determining a threshold surface in the 3-dimensional space separating the cytoplasmic and nuclear regions. In another aspect, the neural network comprises a hardware-encoded algorithm in the form of a look-up table.

摘要翻译： 神经网络辅助多光谱分割方法和系统。 根据本发明，针对生物样品的相同显微照相场景获取具有不同光谱带的三个图像。 处理图像并产生识别细胞材料的细胞材料图。 然后将细胞材料图应用于神经网络。 神经网络通过确定分离细胞质和核区域的三维空间中的阈值表面将细胞材料图分类为核对象和细胞质对象。 另一方面，神经网络包括以查询表的形式的硬件编码的算法。

公开(公告)号：US06372447B1

公开(公告)日：2002-04-16

申请号：US09389217

申请日：1999-09-03

申请人： Ryan S. Raz

发明人： Ryan S. Raz

IPC分类号： C12Q124

CPC分类号： G01N1/40 ,  G01N1/2813 ,  G01N15/0618 ,  G01N2015/0065

摘要： An apparatus and method are provided for preparing specimens of biological cells uniformly distributed over a substrate surface. The method comprises providing a suspension of biological cells in fluid, and then extracting biological target cells from the suspension to leave debris and contaminants behind. The extracted target cells are distributed over a substrate surface into a primary layer of cells coupled to the substrate surface with the remaining cells forming a secondary layer on top of the primary layer. The target cells forming the secondary layer are then removed to produce a substantially non-overlapping layer of target cells coupled to the substrate surface.

摘要翻译： 提供了一种用于制备均匀分布在基底表面上的生物细胞样品的装置和方法。 该方法包括提供流体中的生物细胞的悬浮液，然后从悬浮液中提取生物靶细胞以留下碎片和污染物。 提取的靶细胞在衬底表面上分布成耦合到衬底表面的细胞的主层，剩余的细胞在主层的顶部上形成第二层。 然后去除形成次级层的靶细胞，以产生与衬底表面耦合的基本上非重叠的靶细胞层。

公开(公告)号：US06323040B1

公开(公告)日：2001-11-27

申请号：US09503905

申请日：2000-02-15

申请人： Ryan S. Raz ,  Gordon Robertson

发明人： Ryan S. Raz ,  Gordon Robertson

IPC分类号： G01N100

CPC分类号： G02B21/34 ,  B01L3/502715 ,  B01L3/5082 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0822 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0487 ,  G01N1/2813 ,  G01N1/40 ,  G01N1/4077 ,  Y10T436/112499 ,  Y10T436/25 ,  Y10T436/25375

摘要： A biological specimen preparation system for processing and depositing a portion of a biological specimen from a specimen vial onto a slide. The biological specimen preparation specimen includes a specimen processing unit and an external actuation unit. The specimen vial is connected to an input port on the specimen processing unit and the slide is coupled to an output port. A portion of the biological specimen is injected into the specimen processing unit and prepared by sequentially passing the biological specimen under the control of the external actuation unit through a number of specimen conditioning chambers and a specimen enrichment chamber in the specimen processing unit. The specimen is then deposited on the slide through the output port. To prevent sample to sample contamination, the specimen processing unit is manufactured as a disposable component using injection moulding techniques.

摘要翻译： 一种生物样品制备系统，用于将生物样品的一部分从样品瓶处理和沉积到载玻片上。 生物样本制剂样本包括样本处理单元和外部致动单元。 样品瓶连接到样品处理单元上的输入端口，并且载玻片耦合到输出端口。 生物样本的一部分被注入到样本处理单元中，并通过在外部致动单元的控制下通过样本处理单元中的多个样本调节室和样本浓缩室依次通过生物样本。 然后将样品通过输出端口沉积在载玻片上。 为了防止样品污染样品，样品处理单元使用注塑技术制造为一次性部件。

公开(公告)号：US6049421A

公开(公告)日：2000-04-11

申请号：US009269

申请日：1998-01-20

申请人： Ryan S. Raz ,  Uri Frodis ,  Duncan A. Newman

发明人： Ryan S. Raz ,  Uri Frodis ,  Duncan A. Newman

IPC分类号： G02B21/00 ,  G02B21/26 ,  G02B21/34 ,  G02B21/36

CPC分类号： G02B21/34 ,  G02B21/002 ,  G02B21/26

摘要： A system is provided for the automated imaging of a succession of microscope slides (S). The slides are mounted in carriers and loaded into cassettes. Slide carriers from a first cassette in a slide loader (2) are ejected from the cassette into a loading station and thence moved by an actuator onto a stage beneath a microscope objective (6) which images a sub-area of a circular sample area on the slide onto an imaging sensor (10) whence images are digitized and stored. The stage (4) is conjointly rotated by a motor (22) as a carrier (24) for the stage is moved linearly, so as to execute a spiral scan of sub-areas of the sample area which sub-areas are successively imaged using a strobe lamp (12) to illuminate the sample area at intervals during the scan determined by signals from an encoder (25). A preliminary scan, which may be in an opposite direction along the spiral, may be used to obtain, for a focus actuator (18), focusing data used during the scan. As a following slide carrier is loaded onto the stage for scanning, the previous slide carrier is ejected into a chute from which an actuator inserts it into a further cassette in the same orientation in which it left the first cassette.

摘要翻译： 提供了一系列用于一系列显微镜幻灯片（S）的自动成像的系统。 载玻片安装在载体中并装入盒中。 从滑块装载机（2）中的第一盒中滑动载架从盒子中弹出到装载站，然后由致动器移动到显微镜物镜（6）下方的台上，该显微镜物镜将圆形样品区域的子区域成像 将图像数字化并存储在成像传感器（10）上。 通过电动机（22）将载物台（4）作为载体（24）共同旋转，使载物台线性地移动，从而对采样区域的子区域进行螺旋扫描，该子区域依次成像，使用 频闪灯（12），用于在由来自编码器（25）的信号确定的扫描期间间隔照射样品区域。 可以使用沿着螺旋沿相反方向的初步扫描，以获得聚焦致动器（18）在扫描期间使用的聚焦数据。 由于以下滑动载体被装载到载物台上用于扫描，所以先前的滑动载体被弹出到滑槽中，致动器将滑动载架从其从其从离开第一盒的相同方向插入到另一盒中。

公开(公告)号：US5535751A

公开(公告)日：1996-07-16

申请号：US361605

申请日：1994-12-22

申请人： Ryan S. Raz

发明人： Ryan S. Raz

IPC分类号： A61B8/14 ,  G01S15/89 ,  G10K11/30 ,  A61B8/00

CPC分类号： A61B8/4483 ,  A61B8/14 ,  G01S15/8925 ,  G01S15/8936 ,  G10K11/30 ,  Y10S128/916

摘要： An ultrasonic imaging system incorporates an imaging device utilizing confocal acoustic focusing of ultrasonic transducers together with apparatus for moving points of focus of the transducers to scan a volume to be imaged and gather data as to the acoustic reflectivity of matter within the volume, the gathered data being used to construct an image of the volume. In a preferred arrangement the point of focus of each transducer is moved in three dimensions. Use of confocal techniques can permit enhanced resolution to be obtained compared with conventional ultrasonic imaging techniques.

摘要翻译： 超声成像系统包括利用超声波换能器的共聚焦声学聚焦的成像装置和用于移动换能器的焦点的扫描要成像的体积的装置的装置，并收集关于体积内的物质的声反射率的数据，所收集的数据 用于构建卷的图像。 在优选的布置中，每个换能器的焦点在三个维度上移动。 与传统的超声成像技术相比，使用共聚焦技术可以获得更高的分辨率。