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1.发明授权Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions 有权在基于DNA聚合酶的合成反应中连续添加短DNA寡核苷酸公开(公告)号：US07871799B2
公开(公告)日：2011-01-18
申请号：US10727779
申请日：2003-12-03
申请人： Shea N. Gardner , Raymond P. Mariella, Jr. , Allen T. Christian , Jennifer A. Young , David S. Clague
发明人： Shea N. Gardner , Raymond P. Mariella, Jr. , Allen T. Christian , Jennifer A. Young , David S. Clague
CPC分类号： C12Q1/6844 , C07H21/00 , C12N15/10 , C12N15/1027 , C12N15/1031 , C12Q1/6813 , C12Q1/686 , C12Q2521/101 , C12Q2533/107 , C12Q2525/143
摘要： A method of fabricating a DNA molecule of user-defined sequence. The method comprises the steps of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an even or odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths. In one embodiment starting sequence fragments are of different lengths, n, n+1, n+2, etc.
摘要翻译： 制备用户定义序列的DNA分子的方法。 该方法包括以下步骤：预先选择将包含用户定义序列的DNA分子的多个DNA序列片段，以及将多个DNA序列片段与至少一种聚合酶结合， DNA序列片段连接以产生用户定义序列的DNA分子。 序列段可以是长度为n，其中n是偶或奇整数。 在一个实施例中，期望的杂交重叠的长度由用户指定，并且用于组合它们的序列和协议由计算（生物信息学）预测指导。 在一个实施例中，序列片段从多个读取框架组合以跨越序列的相同区域，使得可以以不同的重叠长度发生多个期望的杂交。 在一个实施方案中，起始序列片段具有不同长度，n，n + 1，n + 2等
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2.发明申请Sequential Addition of Short DNA Oligos In DNA-Polymerase-Based Synthesis Reactions 有权基于DNA聚合酶的合成反应中短DNA寡核苷酸的连续添加公开(公告)号：US20110104763A1
公开(公告)日：2011-05-05
申请号：US12959789
申请日：2010-12-03
申请人： Shea N. Gardner , Raymond P. Mariella, JR. , Allen T. Christian , Jennifer A. Young , David S. Clague
发明人： Shea N. Gardner , Raymond P. Mariella, JR. , Allen T. Christian , Jennifer A. Young , David S. Clague
IPC分类号： C12P19/34
CPC分类号： C12Q1/6844 , C07H21/00 , C12N15/10 , C12N15/1027 , C12N15/1031 , C12Q1/6813 , C12Q1/686 , C12Q2521/101 , C12Q2533/107 , C12Q2525/143
摘要： A method of fabricating a DNA molecule of user-defined sequence. The method comprises the steps of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an even or odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths. In one embodiment starting sequence fragments are of different lengths, n, n+1, n+2, etc.
摘要翻译： 制备用户定义序列的DNA分子的方法。 该方法包括以下步骤：预先选择将包含用户定义序列的DNA分子的多个DNA序列片段，以及将多个DNA序列片段与至少一种聚合酶结合， DNA序列片段连接以产生用户定义序列的DNA分子。 序列段可以是长度为n，其中n是偶或奇整数。 在一个实施例中，期望的杂交重叠的长度由用户指定，并且用于组合它们的序列和协议由计算（生物信息学）预测指导。 在一个实施例中，序列片段从多个读取框架组合以跨越序列的相同区域，使得可以以不同的重叠长度发生多个期望的杂交。 在一个实施方案中，起始序列片段具有不同长度，n，n + 1，n + 2等
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3.发明授权Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions 有权在基于DNA聚合酶的合成反应中连续添加短DNA寡核苷酸公开(公告)号：US08470537B2
公开(公告)日：2013-06-25
申请号：US12959789
申请日：2010-12-03
申请人： Shea N. Gardner , Raymond P. Mariella, Jr. , Allen T. Christian , Jennifer A. Young , David S. Clague
发明人： Shea N. Gardner , Raymond P. Mariella, Jr. , Allen T. Christian , Jennifer A. Young , David S. Clague
CPC分类号： C12Q1/6844 , C07H21/00 , C12N15/10 , C12N15/1027 , C12N15/1031 , C12Q1/6813 , C12Q1/686 , C12Q2521/101 , C12Q2533/107 , C12Q2525/143
摘要： A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.
摘要翻译： 预选选择将包含用户定义序列的DNA分子的多个DNA序列片段的方法，将DNA序列片段在时间上分离，并将多个DNA序列片段与至少一种聚合酶结合，其中多个DNA序列片段 加入生产用户定义序列的DNA分子。 序列段可以是长度为n，其中n是奇整数。 在一个实施例中，期望的杂交重叠的长度由用户指定，并且用于组合它们的序列和协议由计算（生物信息学）预测指导。 在一个实施例中，序列片段从多个读取框架组合以跨越序列的相同区域，使得可以以不同的重叠长度发生多个期望的杂交。
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4.发明授权Systems and methods for separating particles and/or substances from a sample fluid 有权用于从样品流体中分离颗粒和/或物质的系统和方法公开(公告)号：US09480935B2
公开(公告)日：2016-11-01
申请号：US12024762
申请日：2008-02-01
申请人： Raymond P. Mariella, Jr. , George M. Dougherty , John M. Dzenitis , Robin R. Miles , David S. Clague
发明人： Raymond P. Mariella, Jr. , George M. Dougherty , John M. Dzenitis , Robin R. Miles , David S. Clague
CPC分类号： B01D17/06 , G01N1/4077 , G01N2001/4072 , G01N2015/0288
摘要： Systems and methods for separating particles and/or toxins from a sample fluid. A method according to one embodiment comprises simultaneously passing a sample fluid and a buffer fluid through a chamber such that a fluidic interface is formed between the sample fluid and the buffer fluid as the fluids pass through the chamber, the sample fluid having particles of interest therein; applying a force to the fluids for urging the particles of interest to pass through the interface into the buffer fluid; and substantially separating the buffer fluid from the sample fluid.
摘要翻译： 用于从样品流体中分离颗粒和/或毒素的系统和方法。 根据一个实施方案的方法包括同时使样品流体和缓冲流体通过室，使得当流体通过室时在样品流体和缓冲流体之间形成流体界面，样品流体具有目标颗粒 ; 向流体施加力以促使感兴趣的颗粒通过界面进入缓冲流体; 并且从样品流体基本上分离缓冲流体。
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5.发明申请Systems and Methods for Separating Particles and/or Substances from a Sample Fluid 有权从样品流体中分离颗粒和/或物质的系统和方法公开(公告)号：US20090194420A1
公开(公告)日：2009-08-06
申请号：US12024762
申请日：2008-02-01
申请人： Raymond P. Mariella, JR. , George M. Dougherty , John M. Dzenitis , Robin R. Miles , David S. Clague
发明人： Raymond P. Mariella, JR. , George M. Dougherty , John M. Dzenitis , Robin R. Miles , David S. Clague
CPC分类号： B01D17/06 , G01N1/4077 , G01N2001/4072 , G01N2015/0288
摘要： Systems and methods for separating particles and/or toxins from a sample fluid. A method according to one embodiment comprises simultaneously passing a sample fluid and a buffer fluid through a chamber such that a fluidic interface is formed between the sample fluid and the buffer fluid as the fluids pass through the chambers the sample fluid having particles of interest therein; applying a force to the fluids for urging the particles of interest to pass through the interface into the buffer fluid; and substantially separating the buffer fluid from the sample fluid.
摘要翻译： 用于从样品流体中分离颗粒和/或毒素的系统和方法。 根据一个实施例的方法包括同时通过样品流体和缓冲流体通过室，使得当流体通过腔室时，在样品流体和缓冲流体之间形成流体界面，其中具有感兴趣的颗粒的样品流体; 向流体施加力以促使感兴趣的颗粒通过界面进入缓冲流体; 并且从样品流体基本上分离缓冲流体。
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6.发明授权Computational method and system for modeling, analyzing, and optimizing DNA amplification and synthesis 失效用于建模，分析和优化DNA扩增和合成的计算方法和系统公开(公告)号：US07711491B2
公开(公告)日：2010-05-04
申请号：US10840779
申请日：2004-05-05
CPC分类号： G06F19/20
摘要： A computational method and computer-based system of modeling DNA synthesis for the design and interpretation of PCR amplification, parallel DNA synthesis, and microarray chip analysis. The method and system include modules that address the bioinformatics, kinetics, and thermodynamics of DNA amplification and synthesis. Specifically, the steps of DNA selection, as well as the kinetics and thermodynamics of DNA hybridization and extensions, are addressed, which enable the optimization of the processing and the prediction of the products as a function of DNA sequence, mixing protocol, time, temperature and concentration of species.
摘要翻译： 一种计算方法和基于计算机的DNA合成建模系统，用于设计和解释PCR扩增，平行DNA合成和微阵列芯片分析。 该方法和系统包括解决DNA扩增和合成的生物信息学，动力学和热力学的模块。 具体来说，解决了DNA选择的步骤，以及DNA杂交和扩增的动力学和热力学，这使得能够优化作为DNA序列，混合方案，时间，温度的函数的产物的处理和预测 和物种的集中。
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7.发明授权Physics-based signal processing algorithms for micromachined cantilever arrays 有权用于微加工悬臂阵列的基于物理的信号处理算法公开(公告)号：US08584506B2
公开(公告)日：2013-11-19
申请号：US11435495
申请日：2006-05-16
申请人： James V. Candy , David S. Clague , Christopher L. Lee , Robert E. Rudd , Alan K. Burnham , Joseph W. Tringe
发明人： James V. Candy , David S. Clague , Christopher L. Lee , Robert E. Rudd , Alan K. Burnham , Joseph W. Tringe
CPC分类号： G01N29/036 , G01N2291/0256
摘要： A method of using physics-based signal processing algorithms for micromachined cantilever arrays. The methods utilize deflection of a micromachined cantilever that represents the chemical, biological, or physical element being detected. One embodiment of the method comprises the steps of modeling the deflection of the micromachined cantilever producing a deflection model, sensing the deflection of the micromachined cantilever and producing a signal representing the deflection, and comparing the signal representing the deflection with the deflection model.
摘要翻译： 一种使用基于物理学的信号处理算法进行微机械悬臂阵列的方法。 该方法利用表示所检测的化学，生物或物理元件的微机械悬臂的偏转。 该方法的一个实施例包括以下步骤：对产生偏转模型的微机械悬臂的偏转进行建模，感测微机械悬臂的偏转并产生表示偏转的信号，并将表示偏转的信号与偏转模型进行比较。
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公开(公告)号：US07504265B2
公开(公告)日：2009-03-17
申请号：US11447754
申请日：2006-06-05
IPC分类号： G01N33/543
CPC分类号： G01N15/1404 , G01N15/1484 , G01N2015/0065 , G01N2015/142 , Y10S436/80 , Y10S436/805 , Y10T436/117497
摘要： A flow cytometer includes a flow cell for detecting the sample, an oil phase in the flow cell, a water phase in the flow cell, an oil-water interface between the oil phase and the water phase, a detector for detecting the sample at the oil-water interface, and a hydrophobic unit operatively connected to the sample. The hydrophobic unit is attached to the sample. The sample and the hydrophobic unit are placed in an oil and water combination. The sample is detected at the interface between the oil phase and the water phase.
摘要翻译： 流式细胞仪包括用于检测样品的流动池，流动池中的油相，流动池中的水相，油相和水相之间的油 - 水界面，用于在该油相和水相之间检测样品的检测器 油 - 水界面和可操作地连接到样品的疏水单元。 疏水单元附着在样品上。 将样品和疏水单元置于油水组合中。 在油相和水相之间的界面处检测样品。
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公开(公告)号：US07081227B2
公开(公告)日：2006-07-25
申请号：US10862998
申请日：2004-06-07
IPC分类号： G01N21/00
CPC分类号： G01N15/1404 , G01N15/1484 , G01N2015/0065 , G01N2015/142 , Y10S436/80 , Y10S436/805 , Y10T436/117497
摘要： A flow cytometer includes a flow cell for detecting the sample, an oil phase in the flow cell, a water phase in the flow cell, an oil-water interface between the oil phase and the water phase, a detector for detecting the sample at the oil-water interface, and a hydrophobic unit operatively connected to the sample. The hydrophobic unit is attached to the sample. The sample and the hydrophobic unit are placed in an oil and water combination. The sample is detected at the interface between the oil phase and the water phase.
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