公开(公告)号：US20240198339A1

公开(公告)日：2024-06-20

申请号：US18595927

申请日：2024-03-05

Applicant: The General Hospital Corporation

Inventor： Ravi Kapur ,  Kyle C. Smith ,  Mehmet Toner

IPC: B01L3/00 ,  A61K35/28 ,  G01N1/40 ,  G01N15/00 ,  G01N15/01 ,  G01N15/02 ,  G01N15/06 ,  G01N15/14 ,  G01N15/149

CPC classification number: B01L3/502761 ,  A61K35/28 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/0618 ,  G01N15/1484 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N2001/4088 ,  G01N2015/0053 ,  G01N15/01 ,  G01N2015/0288 ,  G01N2015/1486 ,  G01N15/149 ,  G01N2015/1493

Abstract: A microfluidic device includes: a first microfluidic channel; a second microfluidic channel extending along the first microfluidic channel; and a first array of islands separating the first microfluidic channel from the second microfluidic channel, in which each island is separated from an adjacent island in the array by an opening that fluidly couples the first microfluidic channel to the second microfluidic channel, in which the first microfluidic channel, the second microfluidic channel, and the islands are arranged so that a fluidic resistance of the first microfluidic channel increases relative to a fluidic resistance of the second microfluidic channel along a longitudinal direction of the first microfluidic channel such that, during use of the microfluidic device, a portion of a fluid sample flowing through the first microfluidic channel passes through one or more of the openings between adjacent islands into the second microfluidic channel.

公开(公告)号：US11959923B2

公开(公告)日：2024-04-16

申请号：US17314254

申请日：2021-05-07

Applicant: Cambridge Enterprise Limited

Inventor： Emma Yates ,  Christopher Dobson ,  Therese Herling ,  Tuomas Pertti Jonathan Knowles

IPC: G01N33/68 ,  B01L3/00 ,  G01N15/02 ,  G01N27/447 ,  G01N33/50 ,  G01N33/52 ,  G01N15/00

CPC classification number: G01N33/6845 ,  B01L3/502753 ,  G01N15/0266 ,  G01N27/447 ,  G01N33/50 ,  G01N33/52 ,  B01L2200/0631 ,  B01L2200/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0883 ,  B01L2400/0421 ,  G01N2015/0038 ,  G01N2015/0288

Abstract: Flow apparatuses comprising a separation channel, a downstream flow separator, a detection zone, an observation zone, and a waste channel. The separation channel has first and second flows in contact and allows lateral movement of components between contacting first and second flows. The downstream flow separator is in communication with the separation channel and diverts a part of the first fluid flow, the second fluid flow, or both, from the separation channel. The detection zone comprises a detection channel downstream of and in communication with the flow separator and configured to receive a plurality of diverted flows from the flow separator and a label channel configured to label the diverted flows from the flow separator. The observation zone is configured to record an analytical signal indicative of the quantity and the electrical properties of the component. The waste channel is at the downstream end of the observation zone.

公开(公告)号：US11925934B2

公开(公告)日：2024-03-12

申请号：US16918349

申请日：2020-07-01

Applicant: GEORGIA TECH RESEARCH CORPORATION

Inventor： Todd Sulchek ,  Alexander Alexeev ,  Gonghao Wang

IPC: B01L3/00 ,  G01N15/02 ,  G01N33/48 ,  G01N33/49 ,  G01N33/50

CPC classification number: B01L3/502761 ,  G01N15/0272 ,  G01N33/48 ,  G01N33/5005 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/086 ,  G01N15/01 ,  G01N2015/0288 ,  G01N33/491

Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.

公开(公告)号：US11821828B1

公开(公告)日：2023-11-21

申请号：US18069189

申请日：2022-12-20

Applicant: KUWAIT UNIVERSITY

Inventor： Naser Alsayegh ,  Amman M. Bahman

IPC: G01N15/02 ,  B01L3/00

CPC classification number: G01N15/0272 ,  B01L3/502761 ,  B01L2300/0681 ,  G01N2015/0288

Abstract: The system and method for determining physical stability of dispersed particles in flowing liquid suspensions is performed by passing a fluid with suspended particulates in a column of filter elements having progressively smaller porosities. The fluid is received by a fluid supply receptacle and then passed through the column of filter elements. The filter elements are arranged so that successive filters are downstream of each other, with successive filters having successively smaller porosities. The porosity of an initial filter element has a sufficiently large porosity to filter a largest particle size intended for detection, and a final filter element has at least a sufficiently small porosity to filter a smallest particle size intended for detection. This results in the successive filters separating successively smaller particles from the fluid. Average particle size and particle count are obtained by removing a mesh filter element and performing SEM analysis on the filtered particles.

公开(公告)号：US11740172B2

公开(公告)日：2023-08-29

申请号：US17580557

申请日：2022-01-20

Applicant: AEROVIRUS TECHNOLOGIES INC.

Inventor： Norman Marchand ,  Carmine Graziano

IPC: G01N15/06 ,  G01N15/02 ,  B01D11/04 ,  G01N27/00 ,  B01L9/00 ,  B01L3/00 ,  B01L5/00 ,  G01N33/569 ,  C12Q1/70 ,  G01N15/00 ,  G01N27/447 ,  G01N15/04 ,  C12Q1/6804

CPC classification number: G01N15/0656 ,  B01D11/0496 ,  B01L3/50273 ,  B01L3/502715 ,  B01L5/00 ,  B01L9/527 ,  G01N15/0255 ,  G01N15/0266 ,  G01N15/042 ,  G01N15/0606 ,  G01N27/00 ,  G01N33/56983 ,  B01L2300/0636 ,  C12Q1/6804 ,  C12Q1/70 ,  G01N27/44791 ,  G01N2015/0065 ,  G01N2015/0288 ,  C12Q1/6804 ,  C12Q2525/205 ,  C12Q2563/116 ,  C12Q2565/629 ,  C12Q1/70 ,  C12Q2525/205 ,  C12Q2563/116 ,  C12Q2565/629

Abstract: Disclosed herein are devices and methods for the real-time detection of aeropathogens. The device includes an aerosampler having an air inlet and at least one collector tube, a microfluidic system which includes a container, piping, a micro-pump for flowing a liquid, and a viral detection chamber. The viral detection chamber has an electrode which may be equipped with functionalized biosensors, a counter electrode, an electronic detection system connectable to the electrodes of the viral detection chamber, and an embedded electronic processing system for processing data from the electronic detection system.

公开(公告)号：US20230191398A1

公开(公告)日：2023-06-22

申请号：US18165434

申请日：2023-02-07

Applicant: International Business Machines Corporation

Inventor： Evan Colgan ,  Joshua T. Smith ,  Benjamin Wunsch

IPC: B01L3/00 ,  B81C1/00 ,  B81B7/00 ,  G01N15/02

CPC classification number: B01L3/502707 ,  B81C1/00309 ,  B81C1/00111 ,  B81B7/0061 ,  G01N15/0255 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2300/12 ,  B81B2203/0361 ,  B81B2201/058 ,  G01N2015/0288 ,  B01L2200/0689 ,  B01L2200/0652 ,  B01L3/502753 ,  B01L3/502723

Abstract: A method for fabricating a fluidic device includes depositing a sacrificial material on a pillar array arranged on a substrate. The method also includes removing a portion of the sacrificial material. The method further includes depositing a sealing layer on the pillar array to form a sealed fluidic cavity.

公开(公告)号：US11648557B2

公开(公告)日：2023-05-16

申请号：US16581837

申请日：2019-09-25

Applicant: International Business Machines Corporation

Inventor： Huan Hu ,  Joshua T. Smith ,  Gustavo A. Stolovitzky ,  Benjamin H. Wunsch

IPC: B01L3/00 ,  H01L21/3105 ,  H01L21/3213 ,  H01L29/06 ,  B82Y5/00 ,  G01N15/02 ,  B82Y15/00 ,  H01L21/306 ,  G01N15/00

CPC classification number: B01L3/502753 ,  B82Y5/00 ,  B82Y15/00 ,  G01N15/0255 ,  H01L21/31055 ,  H01L21/31056 ,  H01L21/3213 ,  H01L29/0676 ,  B01L2200/0652 ,  B01L2200/12 ,  B01L2300/0896 ,  B01L2400/086 ,  G01N2015/0053 ,  G01N2015/0065 ,  G01N2015/0288 ,  H01L21/30604

Abstract: Techniques relate to forming a sorting device. A mesh is formed on top of a substrate. Metal assisted chemical etching is performed to remove substrate material of the substrate at locations of the mesh. Pillars are formed in the substrate by removal of the substrate material. The mesh is removed to leave the pillars in a nanopillar array. The pillars in the nanopillar array are designed with a spacing to sort particles of different sizes such that the particles at or above a predetermined dimension are sorted in a first direction and the particles below the predetermined dimension are sorted in a second direction.

公开(公告)号：US20190017919A1

公开(公告)日：2019-01-17

申请号：US16100726

申请日：2018-08-10

Applicant: The Regents of the University of Michigan

Inventor： Sunitha Nagrath ,  Hyeun Joong Yoon ,  Eric Lin ,  Max S. Wicha ,  Lianette Rivera Baez ,  Diane M. Simeone

IPC: G01N15/10 ,  G01N15/02 ,  B01L3/00 ,  G01N33/50 ,  G01N33/574 ,  G01N15/00

CPC classification number: G01N15/1056 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0883 ,  G01N15/0272 ,  G01N33/5005 ,  G01N33/574 ,  G01N2015/0065 ,  G01N2015/008 ,  G01N2015/0288 ,  G01N2015/1006 ,  G01N2015/1081

Abstract: A microfluidic device for detecting rare cells in a fluid sample comprises the rare cell and other cells. The microfluidic device comprises an inlet for receiving the fluid sample, a labyrinth channel structure in fluid communication with the inlet, and an outlet in fluid communication with the labyrinth channel structure for collecting the rare cells separated from the other cells in the fluid sample. The labyrinth channel structure comprises at least one channel through which the fluid sample flows. The at least one channel has a plurality of segments and a plurality of corners with each corner defined between adjacent segments. The presence of the plurality of corners induces separation of the rare cells from the other cells in the fluid sample as the rare cells move to a first equilibrium position within the at least one channel when a ratio of inertial lift forces (FZ) and Dean flow (FD) of the fluid sample is from 2 to 10.

公开(公告)号：US10077462B2

公开(公告)日：2018-09-18

申请号：US14775341

申请日：2014-03-14

Applicant: THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  THE GENERAL HOSPITAL CORP

Inventor： Han Wei Hou ,  Jongyoon Han ,  Roby Bhattacharyya ,  Deb Hung

IPC: C12Q1/24 ,  B01L3/00 ,  G01N15/02 ,  G01N15/00

CPC classification number: C12Q1/24 ,  B01L3/502753 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/088 ,  B01L2400/0487 ,  G01N15/0255 ,  G01N2015/0065 ,  G01N2015/0288

Abstract: A method and microfluidic device useful for isolating microbes from a blood sample which includes introducing the blood sample into the sample inlet of a spiral microfluidic device; and introducing a second fluid into the sheath inlet of the microfluidic device, wherein the spiral channel terminates in a microbe outlet and a waste outlet, and wherein the spiral channel includes a length, height, and a width that define an aspect ratio adapted to isolate any microbes present in the sample along a first portion of the spiral channel terminating at the microbe outlet, and to isolate red blood cells and leukocytes along a second portion of the spiral channel terminating at the waste outlet; and collecting the microbes from the microbe outlet, thereby isolating the microbes.

公开(公告)号：US20180200717A1

公开(公告)日：2018-07-19

申请号：US15323267

申请日：2015-06-30

Applicant: The Regents of the University of California

Inventor： Daniel K. Yang ,  Lydia Lee Sohn

IPC: B01L3/00 ,  F15D1/14 ,  G01N33/569 ,  G01N15/02

CPC classification number: B01L3/502776 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/123 ,  B01L2400/086 ,  F15D1/14 ,  G01N15/0255 ,  G01N33/56972 ,  G01N2015/0288

Abstract: Devices for separating constituents (e.g., cells) in a fluid sample are provided. The device includes a microfluidic conduit configured to carry a flow of a fluid sample and includes two or more separation elements, each separation element including a first region and a second region, where the first region has a cross-sectional area less than a cross-sectional area of the second region. The device also includes a flow resistive element in fluid communication with the microfluidic conduit in a region between two adjacent separation elements. Also provided are methods of using the devices, as well as systems and kits that include the devices. The devices, systems, methods and kits find use in a variety of different applications, including diagnostic assays.