Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
    2.
    发明申请
    Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 有权
    高山被孢霉二酰基甘油酰基转移酶用于改变多不饱和脂肪酸和含油生物中的油含量

    公开(公告)号:US20060094087A1

    公开(公告)日:2006-05-04

    申请号:US11024545

    申请日:2004-12-29

    摘要: An acyltransferase is provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous organisms. Specifically, the gene encoding diacylglycerol acyltransferase (DGAT2) has been isolated from Mortierella alpina. This gene encodes an enzyme that participates in the terminal step in oil biosynthesis in fungi and yeast and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. Most desirably, the substrate specificity of the instant DGAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

    摘要翻译: 提供了一种酰基转移酶,适用于制造含油生物体中富含ω-脂肪酸的微生物油。 具体地,从高山被孢霉中分离出编码二酰基甘油酰基转移酶(DGAT2)的基因。 该基因编码参与真菌和酵母中油生物合成的最终步骤的酶,并且预期在改变油性生物油中产生的长链多不饱和脂肪酸的量方面起关键作用。 最理想的是,即时DGAT2的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母中积累长链等于或大于C 20的长链PUFA。

    Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
    6.
    发明申请
    Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 失效
    高山被孢霉甘油-3-磷酸O-酰基转移酶用于改变多不饱和脂肪酸和含油生物中的油含量

    公开(公告)号:US20060094091A1

    公开(公告)日:2006-05-04

    申请号:US11254173

    申请日:2005-10-19

    摘要: Glycerol-3-phosphate o-acyltransferase (GPAT) participates in the first step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids (PUFAs) produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment isolated from Mortierella alpina encoding a GPAT that is suitable for use in the manufacture of oils enriched in omega fatty acids in oleaginous organisms. Most desirably, the substrate specificity of the instant GPAT will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

    摘要翻译: 甘油-3-磷酸O-酰基转移酶(GPAT)参与石油生物合成的第一步,预计在改变含油生物油中产生的长链多不饱和脂肪酸(PUFA)的量方面发挥关键作用。 本申请提供了从编码GPAT的高山被孢霉分离的核酸片段,该GPAT适用于制造含油生物体中富含ω-脂肪酸的油。 最理想的是,本发明的GPAT的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母(Yarrowia lipolytica)中积累具有等于或大于C 20的链长度的长链PUFA。