公开(公告)号：US20050229268A1

公开(公告)日：2005-10-13

申请号：US10998525

申请日：2004-11-29

申请人： Stephen Allen ,  Guo-Hua Miao ,  Elmer Heppard ,  Daniel Macool ,  Timothy Helentjaris ,  Jonathan Lightner ,  J. Rafalski ,  Zude Weng ,  Hajime Sakai

发明人： Stephen Allen ,  Guo-Hua Miao ,  Elmer Heppard ,  Daniel Macool ,  Timothy Helentjaris ,  Jonathan Lightner ,  J. Rafalski ,  Zude Weng ,  Hajime Sakai

IPC分类号： C07K14/415 ,  C12N9/12 ,  C12N15/54 ,  C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04

CPC分类号： C12N9/1205 ,  C07K14/415 ,  C12N15/8209 ,  C12N15/8241

摘要： This invention relates to an isolated nucleic acid fragment encoding a carbon catabolite repression polypeptide. The invention also relates to the construction of a chimeric gene encoding all or a portion of the carbon catabolite repression polypeptide, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the carbon catabolite repression polypeptide in a transformed host cell.

摘要翻译： 本发明涉及编码碳分解代谢物抑制多肽的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分碳分解代谢物抑制多肽的嵌合基因，其中嵌合基因的表达导致转化的碳分解代谢物抑制多肽的水平发生改变 宿主细胞。

公开(公告)号：US20060094087A1

公开(公告)日：2006-05-04

申请号：US11024545

申请日：2004-12-29

申请人： Zhixiong Xue ,  Narendra Yadav ,  Daniel Macool

发明人： Zhixiong Xue ,  Narendra Yadav ,  Daniel Macool

IPC分类号： C12P7/64 ,  C07H21/04 ,  C12P21/06 ,  C12N9/10 ,  C12N15/74 ,  C12N1/18

CPC分类号： C12P7/6472 ,  C12N9/1029 ,  C12P7/6463

摘要： An acyltransferase is provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous organisms. Specifically, the gene encoding diacylglycerol acyltransferase (DGAT2) has been isolated from Mortierella alpina. This gene encodes an enzyme that participates in the terminal step in oil biosynthesis in fungi and yeast and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. Most desirably, the substrate specificity of the instant DGAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

摘要翻译： 提供了一种酰基转移酶，适用于制造含油生物体中富含ω-脂肪酸的微生物油。 具体地，从高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT2）的基因。 该基因编码参与真菌和酵母中油生物合成的最终步骤的酶，并且预期在改变油性生物油中产生的长链多不饱和脂肪酸的量方面起关键作用。 最理想的是，即时DGAT2的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母中积累长链等于或大于C 20的长链PUFA。

公开(公告)号：US20060115881A1

公开(公告)日：2006-06-01

申请号：US11265761

申请日：2005-11-02

申请人： Howard Damude ,  Peter Gillies ,  Daniel Macool ,  Stephen Picataggio ,  Dana Pollak ,  James Ragghianti ,  Zhixiong Xue ,  Narendra Yadav ,  Hongxiang Zhang ,  Quinn Zhu

发明人： Howard Damude ,  Peter Gillies ,  Daniel Macool ,  Stephen Picataggio ,  Dana Pollak ,  James Ragghianti ,  Zhixiong Xue ,  Narendra Yadav ,  Hongxiang Zhang ,  Quinn Zhu

IPC分类号： C12P7/64 ,  C07H21/04 ,  C12N9/04 ,  C12N1/18 ,  C12N15/74

CPC分类号： C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/746 ,  C12N15/815 ,  C12P7/6472 ,  A23V2250/187 ,  A23V2200/30

摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.

公开(公告)号：US20060094092A1

公开(公告)日：2006-05-04

申请号：US11264784

申请日：2005-11-01

申请人： Howard Damude ,  Peter Gillies ,  Daniel Macool ,  Stephen Picataggio ,  Dana Pollak ,  James Ragghianti ,  Zhixiong Xue ,  Narendra Yadav ,  Hongxiang Zhang ,  Quinn Zhu

发明人： Howard Damude ,  Peter Gillies ,  Daniel Macool ,  Stephen Picataggio ,  Dana Pollak ,  James Ragghianti ,  Zhixiong Xue ,  Narendra Yadav ,  Hongxiang Zhang ,  Quinn Zhu

IPC分类号： C12P7/64 ,  C12N15/74 ,  C12N1/18

CPC分类号： C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/746 ,  C12N15/815 ,  C12P7/6472 ,  A23V2250/187 ,  A23V2200/30

摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 10% arachidonic acid (ARA, an ω-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of ARA. Production host cells are claimed, as are methods for producing ARA within said host cells.

公开(公告)号：US20060110806A1

公开(公告)日：2006-05-25

申请号：US11264737

申请日：2005-11-01

申请人： Howard Damude ,  Peter Gillies ,  Daniel Macool ,  Stephen Picataggio ,  James Ragghianti ,  John Seip ,  Zhixiong Xue ,  Narendra Yadav ,  Hongxiang Zhang ,  Quinn Zhu

发明人： Howard Damude ,  Peter Gillies ,  Daniel Macool ,  Stephen Picataggio ,  James Ragghianti ,  John Seip ,  Zhixiong Xue ,  Narendra Yadav ,  Hongxiang Zhang ,  Quinn Zhu

IPC分类号： C07H21/04 ,  C12P7/64 ,  C12N9/04 ,  C12N1/18 ,  C12N15/74

CPC分类号： C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/746 ,  C12N15/815 ,  C12P7/6472 ,  A23V2250/187 ,  A23V2200/30

摘要： An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.

公开(公告)号：US20060094091A1

公开(公告)日：2006-05-04

申请号：US11254173

申请日：2005-10-19

申请人： Daniel Macool ,  Zhixiong Xue

发明人： Daniel Macool ,  Zhixiong Xue

IPC分类号： C12P7/64 ,  C07H21/04 ,  C12P21/06 ,  C12N9/10 ,  C12N15/74 ,  C12N1/18

CPC分类号： C12P7/6472 ,  C12N9/1029 ,  C12P7/6463

摘要： Glycerol-3-phosphate o-acyltransferase (GPAT) participates in the first step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids (PUFAs) produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment isolated from Mortierella alpina encoding a GPAT that is suitable for use in the manufacture of oils enriched in omega fatty acids in oleaginous organisms. Most desirably, the substrate specificity of the instant GPAT will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

摘要翻译： 甘油-3-磷酸O-酰基转移酶（GPAT）参与石油生物合成的第一步，预计在改变含油生物油中产生的长链多不饱和脂肪酸（PUFA）的量方面发挥关键作用。 本申请提供了从编码GPAT的高山被孢霉分离的核酸片段，该GPAT适用于制造含油生物体中富含ω-脂肪酸的油。 最理想的是，本发明的GPAT的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母（Yarrowia lipolytica）中积累具有等于或大于C 20的链长度的长链PUFA。