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    DIACYLGLYCEROL ACYLTRANSFERASES FOR ALTERATION OF POLYUNSATURATED FATTY ACIDS AND OIL CONTENT IN OLEAGINOUS ORGANISMS
    4.
    发明申请
    DIACYLGLYCEROL ACYLTRANSFERASES FOR ALTERATION OF POLYUNSATURATED FATTY ACIDS AND OIL CONTENT IN OLEAGINOUS ORGANISMS 审中-公开
    用于改变脂肪酸和脂肪酸含量的二油酸甘油酯

    公开(公告)号：US20080020415A1

    公开(公告)日：2008-01-24

    申请号：US11830023

    申请日：2007-07-30

    申请人： Narendra Yadav ,  Zhixiong Xue ,  Hongxiang Zhang

    发明人： Narendra Yadav ,  Zhixiong Xue ,  Hongxiang Zhang

    IPC分类号： C12P7/64 ,  C07K14/00 ,  C12N15/12 ,  C40B30/04 ,  C12Q1/48 ,  C12N9/10

    CPC分类号： C12N9/1029 ,  C12P7/6463 ,  C12P7/6472

    摘要： Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.

    摘要翻译： 提供了酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω脂肪酸的微生物油。 具体来说，从解脂耶氏酵母和高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT1）的基因。 这些基因编码酵母中参与油生物合成的最终步骤的酶。 预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。

    Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
    5.
    发明申请
    Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 有权
    高山被孢霉二酰基甘油酰基转移酶用于改变多不饱和脂肪酸和含油生物中的油含量

    公开(公告)号：US20060094087A1

    公开(公告)日：2006-05-04

    申请号：US11024545

    申请日：2004-12-29

    申请人： Zhixiong Xue ,  Narendra Yadav ,  Daniel Macool

    发明人： Zhixiong Xue ,  Narendra Yadav ,  Daniel Macool

    IPC分类号： C12P7/64 ,  C07H21/04 ,  C12P21/06 ,  C12N9/10 ,  C12N15/74 ,  C12N1/18

    CPC分类号： C12P7/6472 ,  C12N9/1029 ,  C12P7/6463

    摘要： An acyltransferase is provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous organisms. Specifically, the gene encoding diacylglycerol acyltransferase (DGAT2) has been isolated from Mortierella alpina. This gene encodes an enzyme that participates in the terminal step in oil biosynthesis in fungi and yeast and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. Most desirably, the substrate specificity of the instant DGAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

    摘要翻译： 提供了一种酰基转移酶，适用于制造含油生物体中富含ω-脂肪酸的微生物油。 具体地，从高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT2）的基因。 该基因编码参与真菌和酵母中油生物合成的最终步骤的酶，并且预期在改变油性生物油中产生的长链多不饱和脂肪酸的量方面起关键作用。 最理想的是，即时DGAT2的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母中积累长链等于或大于C 20的长链PUFA。

    Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
    6.
    发明申请
    Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 失效
    用于改变多不饱和脂肪酸的二酰基甘油酰基转移酶和含油生物体中的油含量

    公开(公告)号：US20060094086A1

    公开(公告)日：2006-05-04

    申请号：US11024544

    申请日：2004-12-29

    申请人： Narendra Yadav ,  Zhixiong Xue ,  Hongxiang Zhang

    发明人： Narendra Yadav ,  Zhixiong Xue ,  Hongxiang Zhang

    IPC分类号： C12P7/64 ,  C12P21/06 ,  C07H21/04 ,  C12N9/10 ,  C12N1/18 ,  C12N15/74

    CPC分类号： C12N9/1029 ,  C12P7/6463 ,  C12P7/6472

    摘要： Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.

    摘要翻译： 提供了酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω脂肪酸的微生物油。 具体来说，从解脂耶氏酵母和高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT1）的基因。 这些基因编码酵母中参与油生物合成的最终步骤的酶。 预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。

    Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms
    7.
    发明申请
    Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms 失效
    酰基转移酶调节以增加含油生物的总脂质和油中多不饱和脂肪酸的百分比

    公开(公告)号：US20060094088A1

    公开(公告)日：2006-05-04

    申请号：US11190750

    申请日：2005-07-27

    申请人： Stephen Picataggio ,  Narendra Yadav ,  Hongxiang Zhang

    发明人： Stephen Picataggio ,  Narendra Yadav ,  Hongxiang Zhang

    IPC分类号： C12P7/64 ,  C07H21/04 ,  C12N9/10 ,  C12N15/74 ,  C12N1/18

    CPC分类号： C12N9/1029 ,  C12N15/8247 ,  C12P7/6472

    摘要： Methods to increase the percent of polyunsaturated fatty acids (PUFAs) within the total lipids and oils of PUFA-producing oleaginous organisms are provided herein, by regulating the activity of specific acyltransferases. Specifically, since oil biosynthesis is expected to compete with polyunsaturation during oleaginy, it is possible to reduce or inactivate the activity of an organism's DAG ATs (e.g., phospholipid:diacylglycerol acyltransferase (PDAT) and/or diacylglycerol acyltransferase 1 (DGAT1) and/or diacylglycerol acyltransferase 2 (DGAT2)) to thereby reduce the overall rate of oil biosynthesis while concomitantly increasing the percent of PUFAs that are incorporated into the lipid and oil fractions. The teachings herein will thereby enable one to engineer a wide variety of oleaginous organisms to produce oils with very specific fatty acid compositions.

    摘要翻译： 本文通过调节特异性酰基转移酶的活性来提高增加多不饱和脂肪酸（PUFA）在产生PUFA的含油生物体的总脂质和油中的百分数。 具体地说，由于油生物合成预期在含油期间与多不饱和度竞争，有可能减少或失活生物体的DAG AT（例如磷脂：二酰基甘油酰基转移酶（PDAT）和/或二酰基甘油酰基转移酶1（DGAT1））和/或 二酰基甘油酰基转移酶2（DGAT2）），从而降低油的合成速率，同时增加掺入脂质和油馏分中的PUFA的百分比。 因此，本文的教导使得能够设计各种含油生物以产生具有非常特定的脂肪酸组成的油。

    Delta 12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
    8.
    发明申请
    Delta 12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast 失效
    适用于改变含油酵母中多不饱和脂肪酸水平的Delta 12去饱和酶

    公开(公告)号：US20050216975A1

    公开(公告)日：2005-09-29

    申请号：US10985691

    申请日：2004-11-10

    申请人： Narendra Yadav ,  Quinn Zhu ,  Hongxiang Zhang

    发明人： Narendra Yadav ,  Quinn Zhu ,  Hongxiang Zhang

    IPC分类号： A01H1/00 ,  C12N20060101 ,  C12N1/18 ,  C12N9/02 ,  C12N9/04 ,  C12N15/74 ,  C12N15/82 ,  C12P7/64

    CPC分类号： C12P7/6472 ,  C12N9/0083 ,  C12N15/8247 ,  C12P7/6427

    摘要： The present invention relates to fungal Δ12 fatty acid desaturases that are able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific ω-3 and ω-6 fatty acids by over-expression of the Δ12 fatty acid desaturases are also described herein.

    摘要翻译： 本发明涉及能够催化油酸转化为亚油酸（LA; 18：2）的真菌Delta12脂肪酸去饱和酶。 描述了编码去饱和酶的核酸序列，与其杂交的核酸序列，包含去饱和酶基因的DNA构建体和表达增加的去饱和酶水平的重组宿主微生物。 本文还描述了通过过表达Delta12脂肪酸去饱和酶增加特定ω-3和ω-6脂肪酸的产生的方法。

    Production of polyunsaturated fatty acids in oleaginous yeasts
    9.
    发明申请
    Production of polyunsaturated fatty acids in oleaginous yeasts 有权
    在含油酵母中生产多不饱和脂肪酸

    公开(公告)号：US20070207528A1

    公开(公告)日：2007-09-06

    申请号：US11714377

    申请日：2007-03-05

    申请人： Stephen Picataggio ,  Narendra Yadav ,  Quinn Zhu

    发明人： Stephen Picataggio ,  Narendra Yadav ,  Quinn Zhu

    IPC分类号： C12P7/64 ,  C12N9/04 ,  C12N1/18 ,  C12N15/74

    CPC分类号： C12N15/815 ,  C12P7/6427 ,  C12P7/6472

    摘要： The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of Yarrowia for synthesis of ARA and EPA.

    摘要翻译： 本发明涉及在含油酵母中生产ω-3和/或ω-6脂肪酸的方法。 因此，能够催化亚油酸（LA）转化为γ-亚麻酸（GLA）的去饱和酶和延伸酶; α-亚油酸（ALA）至十八碳四烯酸（STA）; GLA至二氢-γ-亚油酸（DGLA）; STA至二十碳四烯酸（ETA）; DGLA对花生四烯酸（ARA）; ETA至二十碳五烯酸（EPA）; DGLA到ETA; EPA至二十二碳五烯酸（DPA）; 并且ARA至EPA已被引入耶氏酵母的基因组合成ARA和EPA。

    Production of polyunsaturated fatty acids in oleaginous yeasts
    10.
    发明申请
    Production of polyunsaturated fatty acids in oleaginous yeasts 有权
    在含油酵母中生产多不饱和脂肪酸

    公开(公告)号：US20050136519A1

    公开(公告)日：2005-06-23

    申请号：US10840579

    申请日：2004-05-06

    申请人： Stephen Picataggio ,  Narendra Yadav ,  Quinn Zhu

    发明人： Stephen Picataggio ,  Narendra Yadav ,  Quinn Zhu

    IPC分类号： C12N20060101 ,  C12N1/18 ,  C12N9/04 ,  C12N15/74 ,  C12P7/64

    CPC分类号： C12N15/815 ,  C12P7/6427 ,  C12P7/6472

    摘要： The present invention relates to methods for the production of ω-3 and/or ω-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to γ-linolenic acid (GLA); α-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-γ-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of Yarrowia for synthesis of ARA and EPA.

    摘要翻译： 本发明涉及在含油酵母中生产ω-3和/或ω-6脂肪酸的方法。 因此，能够催化亚油酸（LA）转化为γ-亚麻酸（GLA）的去饱和酶和延伸酶; α-亚油酸（ALA）至十八碳四烯酸（STA）; GLA至二氢-γ-亚油酸（DGLA）; STA至二十碳四烯酸（ETA）; DGLA对花生四烯酸（ARA）; ETA至二十碳五烯酸（EPA）; DGLA到ETA; EPA至二十二碳五烯酸（DPA）; 并且ARA至EPA已被引入耶氏酵母的基因组合成ARA和EPA。