公开(公告)号：US20190255217A1

公开(公告)日：2019-08-22

申请号：US16399068

申请日：2019-04-30

申请人： ISE Professional Testing & Consulting Services, Inc.

发明人： Ryanne Early

IPC分类号： A61L27/36 ,  A61L27/38 ,  A61L27/52 ,  C12N5/071 ,  A61K9/70 ,  A61L27/60 ,  A61L27/54 ,  A61K35/65 ,  A61L27/58 ,  A61K35/12 ,  A61K38/17

摘要： The growth factor profile, connective tissue matrix constituents, and immunoprivileged status of urodele extracellular matrix (ECM) and accompanying cutaneous tissue, plus the presence of antimicrobial peptides there, render urodele-derived tissue an ideal source for biological scaffolds for xenotransplantation. In particular, a biological scaffold biomaterial can be obtained by a process that entails (A) obtaining a tissue sample from a urodele, where the tissue comprises ECM, inclusive of the basement membrane, and (B) subjecting the tissue sample to a decellularization process that maintains the structural and functional integrity of the extracellular matrix, by virtue of retaining its fibrous and non-fibrous proteins, glycoaminoglycans (GAGs) and proteoglycans, while removing sufficient cellular components of the sample to reduce or eliminate antigenicity and immunogenicity for xenograft purposes. The resultant urodele-derived biomaterial can be used to enhance restoration of skin homeostasis, to reduce the severity, duration and associated damage caused by post-surgical inflammation, and to promote progression of natural healing and regeneration processes. In addition, the biomaterial promotes the formation of remodeled tissue that is comparable in quality, function, and compliance to undamaged human tissue.

公开(公告)号：US20150352257A1

公开(公告)日：2015-12-10

申请号：US14759895

申请日：2014-01-09

申请人： ISE Professional Testing & Consulting Services, Inc.

发明人： Ryanne Early

IPC分类号： A61L27/36 ,  A61K9/70 ,  A61L27/58 ,  A61K35/12 ,  A61L27/54 ,  A61L27/38 ,  A61K38/17 ,  A61K35/65 ,  A61L27/60

CPC分类号： A61L27/3687 ,  A61K9/70 ,  A61K35/12 ,  A61K35/65 ,  A61K38/1703 ,  A61L27/3604 ,  A61L27/362 ,  A61L27/3625 ,  A61L27/3633 ,  A61L27/3691 ,  A61L27/38 ,  A61L27/52 ,  A61L27/54 ,  A61L27/58 ,  A61L27/60 ,  A61L2300/252 ,  A61L2300/412 ,  A61L2300/604 ,  A61L2300/606 ,  A61L2300/64 ,  A61L2430/34 ,  A61L2430/40 ,  C12N5/0625 ,  C12N2533/90 ,  C12N2533/92

摘要： The growth factor profile, connective tissue matrix constituents, and immunoprivileged status of urodele extracellular matrix (ECM) and accompanying cutaneous tissue, plus the presence of antimicrobial peptides there, render urodele-derived tissue an ideal source for biological scaffolds for xenotransplantation. In particular, a biological scaffold biomaterial can be obtained by a process that entails (A) obtaining a tissue sample from a urodele, where the tissue comprises ECM, inclusive of the basement membrane, and (B) subjecting the tissue sample to a decellularization process that maintains the structural and functional integrity of the extracellular matrix, by virtue of retaining its fibrous and non-fibrous proteins, glycoaminoglycans (GAGs) and proteoglycans, while removing sufficient cellular components of the sample to reduce or eliminate antigenicity and immunogenicity for xenograft purposes. The resultant urodele-derived biomaterial can be used to enhance restoration of skin homeostasis, to reduce the severity, duration and associated damage caused by post-surgical inflammation, and to promote progression of natural healing and regeneration processes. In addition, the biomaterial promotes the formation of remodeled tissue that is comparable in quality, function, and compliance to undamaged human tissue.

摘要翻译： Urodele细胞外基质（ECM）和伴随皮肤组织的生长因子分布，结缔组织基质成分和免疫缺陷状态加上抗微生物肽的存在使得urodele衍生组织成为用于异种移植的生物支架的理想来源。 特别地，生物支架生物材料可以通过以下方法获得：（A）从尿布获得组织样品，其中组织包括ECM，包括基底膜，和（B）使组织样品经脱细胞化过程 通过保留其纤维和非纤维蛋白，糖胺聚糖（GAG）和蛋白多糖来维持细胞外基质的结构和功能完整性，同时去除样品的足够的细胞组分以减少或消除异种移植物目的的抗原性和免疫原性。 所得到的urodele衍生的生物材料可用于增强皮肤稳态的恢复，以减少手术后炎症引起的严重程度，持续时间和相关的损伤，并促进自然愈合和再生过程的进展。 此外，生物材料促进了改性组织的形成，其质量，功能和对未损伤的人体组织的依从性。