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    Nucleic acid and polypeptide sequences useful as adjuvants
    8.
    发明授权
    Nucleic acid and polypeptide sequences useful as adjuvants 失效
    用作佐剂的核酸和多肽序列

    公开(公告)号：US06752995B2

    公开(公告)日：2004-06-22

    申请号：US10123058

    申请日：2002-04-15

    申请人： Stephen A. Johnston ,  Michael J. McGuire

    发明人： Stephen A. Johnston ,  Michael J. McGuire

    IPC分类号： A61K4500

    CPC分类号： C07K14/005 ,  A61K38/162 ,  A61K39/00 ,  A61K39/12 ,  A61K39/275 ,  A61K39/39 ,  A61K2039/53 ,  A61K2039/55516 ,  A61K2039/55561 ,  C07K19/00 ,  C07K2319/00 ,  C12N2710/24022 ,  C12N2710/24034 ,  C12N2710/24222 ,  C12N2710/24234 ,  C12N2760/16122

    摘要： In the present invention parapox genomes were screened using a novel expression strategy to test genes for dendritic cell recruitment activity. One gene was identified, designated as B2WL, that induces dendritic cell accumulation when expressed in skin. In additional testing a second gene, PP30, was identified that exhibited adjuvant activity in the absence of stimulating dendritic cell accumulation at the site of inoculation. When co-inoculated with an antigen-encoding plasmid, both genes acted as adjuvants in stimulating an antibody response to antigens. Furthermore, nucleic acids encoding the identified B2WL peptide adjuvant enhanced the level of protection against viral infection provided by immunization with an HA-expression plasmid. Thus, novel adjuvants for genetic immunization are identified. The invention also demonstrates the application of the linear expression element (LEE) technology for screening genomes of pathogens that may contain genes that are useful when expressed and tested in a novel context.

    摘要翻译： 在本发明中，使用新的表达策略筛选旁分泌基因组以测试树突状细胞募集活性的基因。 一个基因被鉴定，称为B2WL，当在皮肤中表达时诱导树突状细胞的积累。 在另外的测试中，鉴定了在接种位点处没有刺激树突状细胞积累的情况下显示出辅助活性的第二基因PP30。 当用抗原编码质粒共同接种时，两种基因都作为刺激抗体对抗体的抗体的佐剂。 此外，编码所识别的B2WL肽佐剂的核酸增强了用HA表达质粒免疫提供的针对病毒感染的保护水平。 因此，鉴定了用于遗传免疫的新型佐剂。 本发明还证明了线性表达元件（LEE）技术用于筛选可能含有在新颖背景下表达和测试时有用的基因的病原体的基因组的应用。

    Nucleic acid and polypeptide sequences useful as adjuvants
    9.
    发明申请
    Nucleic acid and polypeptide sequences useful as adjuvants 失效
    用作佐剂的核酸和多肽序列

    公开(公告)号：US20030194695A1

    公开(公告)日：2003-10-16

    申请号：US10123058

    申请日：2002-04-15

    发明人： Stephen A. Johnston ,  Michael J. McGuire

    IPC分类号： C12Q001/70 ,  A61K031/70 ,  A01N043/04 ,  C12P021/06 ,  C07K002/00 ,  C07K004/00 ,  C07K005/00 ,  C07K007/00 ,  C07K014/00 ,  C07K016/00 ,  C07K017/00 ,  A61K038/00 ,  C12N005/06 ,  C12N005/16

    CPC分类号： C07K14/005 ,  A61K38/162 ,  A61K39/00 ,  A61K39/12 ,  A61K39/275 ,  A61K39/39 ,  A61K2039/53 ,  A61K2039/55516 ,  A61K2039/55561 ,  C07K19/00 ,  C07K2319/00 ,  C12N2710/24022 ,  C12N2710/24034 ,  C12N2710/24222 ,  C12N2710/24234 ,  C12N2760/16122

    摘要： In the present invention parapox genomes were screened using a novel expression strategy to test genes for dendritic cell recruitment activity. One gene was identified, designated as B2WL, that induces dendritic cell accumulation when expressed in skin. In additional testing a second gene, PP30, was identified that exhibited adjuvant activity in the absence of stimulating dendritic cell accumulation at the site of inoculation. When co-inoculated with an antigen-encoding plasmid, both genes acted as adjuvants in stimulating an antibody response to antigens. Furthermore, nucleic acids encoding the identified B2WL peptide adjuvant enhanced the level of protection against viral infection provided by immunization with an HA-expression plasmid. Thus, novel adjuvants for genetic immunization are identified. The invention also demonstrates the application of the linear expression element (LEE) technology for screening genomes of pathogens that may contain genes that are useful when expressed and tested in a novel context.

    摘要翻译： 在本发明中，使用新的表达策略筛选旁分泌基因组以测试树突状细胞募集活性的基因。 一个基因被鉴定，称为B2WL，当在皮肤中表达时诱导树突状细胞的积累。 在另外的测试中，鉴定了在接种位点处没有刺激树突状细胞积累的情况下显示出辅助活性的第二基因PP30。 当用抗原编码质粒共同接种时，两种基因都作为刺激抗体对抗体的抗体的佐剂。 此外，编码所识别的B2WL肽佐剂的核酸增强了用HA表达质粒免疫提供的针对病毒感染的保护水平。 因此，鉴定了用于遗传免疫的新型佐剂。 本发明还证明了线性表达元件（LEE）技术用于筛选可能含有在新颖背景下表达和测试时有用的基因的病原体的基因组的应用。