公开(公告)号：US20080045762A1

公开(公告)日：2008-02-21

申请号：US11661863

申请日：2005-09-09

申请人： Pat Foody ,  Vijay Anand

发明人： Pat Foody ,  Vijay Anand

IPC分类号： C07C1/00

CPC分类号： B30B9/20 ,  B09B3/00 ,  C12P7/10 ,  C12P19/14 ,  C13K1/02 ,  Y02E50/16 ,  Y02E50/17

摘要： A process for producing a pretreated feedstock is provided. The feedstock is selected from grasses, cereal straws, stover, and combinations thereof, and least about 80% of the feedstock has a particle length of between about 2 cm and about 40 cm. This process comprises wetting the feedstock in liquid, pressing the wet feedstock through one roll press or a series of roll presses to remove at least a portion of water and soluble substances from the wetted feedstock and to shear the feedstock to produce feedstock particles of a size suitable for pumping at a solids concentration of about 8% to about 20% when slurried. At least one roll press, or at least one roll press in the series of roll presses comprises rolls with circumferential v-shaped grooves. The pressed feedstock particles are slurried to produce a slurried feedstock having a consistency of between about 8% and about 20%, and the slurried feedstock pumped into a pretreatment reactor. Dilute acid pretreatment of the slurried feedstock is carried out at a temperature of 160° C. to 280° C.

摘要翻译： 提供了一种生产预处理原料的方法。 原料选自草，谷物秸秆，秸秆及其组合，并且至少约80％的原料具有约2cm至约40cm之间的颗粒长度。 该方法包括将液体中的原料润湿，通过一次辊压机或一系列辊压机挤压湿原料以从被润湿的原料中除去至少一部分水和可溶性物质，并剪切原料以产生尺寸的原料颗粒 适用于在浆液时以约8％至约20％的固体浓度泵送。 在一系列辊压机中至少一个辊压机或至少一个辊压机包括具有周向V形槽的辊。 压制的原料颗粒浆化以产生稠度为约8％至约20％的浆状原料，并将浆化原料泵入预处理反应器。 制浆原料的稀酸预处理在160℃至280℃的温度下进行。

公开(公告)号：US20080041366A1

公开(公告)日：2008-02-21

申请号：US11774113

申请日：2007-07-06

申请人： Daphne Wahnon

发明人： Daphne Wahnon

IPC分类号： C13J1/06 ,  C13K1/02

CPC分类号： C07H3/00 ,  B01D15/1821 ,  B01D15/363 ,  B01J41/05 ,  B01J41/07 ,  B01J49/07 ,  C07C51/47 ,  C07H7/033 ,  C07C53/02 ,  C07C53/08 ,  G01N30/461

摘要： A process for obtaining one or more than one salt of an organic acid(s), or organic acid(s), from an aqueous sugar stream comprising one or more than one mineral acid and the organic acid(s) is provided. The process comprises introducing the aqueous sugar stream to a separation system comprising one or more beds of anion exchange resin and obtaining a stream therefrom comprising the sugar. The one or more beds of anion exchange resin are then regenerated in one or more stages to produce at least one product stream comprising the organic acid, a salt of the organic acid, or a combination thereof, and a separate outlet stream comprising the mineral acid, a salt of the mineral acid, or a combination thereof. The product stream is then recovered. The separation may be conducted with two separation units, or using a single anion exchange unit.

摘要翻译： 提供了从包含一种或多种无机酸和有机酸的糖水流中获得有机酸或有机酸的一种或多于一种的方法。 该方法包括将糖水流引入包含一个或多个阴离子交换树脂床的分离系统，并从其中获得包含糖的料流。 然后一个或多个阴离子交换树脂床在一个或多个阶段中再生，以产生至少一种包含有机酸，有机酸的盐或其组合的产物流，以及包含无机酸的单独出口物流 ，无机酸的盐，或其组合。 然后回收产品流。 分离可以用两个分离单元进行，或者使用单个阴离子交换单元。

公开(公告)号：US07198925B2

公开(公告)日：2007-04-03

申请号：US10953269

申请日：2004-09-30

申请人： Brian Foody

发明人： Brian Foody

IPC分类号： C12P19/02

CPC分类号： C12P19/02 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

摘要： The present invention is directed to a method of pre-treating a lignocellulosic feedstock. The lignocellulosic feedstock comprises cereal straw, stover, or grass. One or more than one bale of lignocellulosic feedstock is conveyed into a pre-treatment reactor. Steam and acid are added to the bales and are maintained at a temperature, acid concentration, and for a time sufficient to hydrolyze hemicellulose to xylose and increase susceptibility of cellulose to digestion by cellulase enzymes, thus producing a pre-treated feedstock. The pre-treated feedstock is then removed from the pre-treatment reactor.

摘要翻译： 本发明涉及预处理木质纤维素原料的方法。 木质纤维素原料包括谷物秸秆，秸秆或草。 一个或多于一个的木质纤维素原料包被输送到预处理反应器中。 将蒸汽和酸加入到包装中，并保持在一定温度，酸浓度下，并且足以将半纤维素水解成木糖并增加纤维素消化纤维素酶的易感性，从而产生预处理的原料。 然后将预处理的原料从预处理反应器中取出。

公开(公告)号：US06939704B1

公开(公告)日：2005-09-06

申请号：US09392476

申请日：1999-09-09

申请人： Theresa C. White ,  Sylvia McHugh ,  Christopher D. Hindle

发明人： Theresa C. White ,  Sylvia McHugh ,  Christopher D. Hindle

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/21 ,  C12N9/24 ,  C12N9/42 ,  C12R1/885 ,  C07H21/04 ,  C12N9/44

CPC分类号： C12Y302/01008 ,  C12N9/2445 ,  C12N2799/021 ,  C12Y302/01021

摘要： The present invention relates to increasing the production of a protein of interest from a fugal host. The invention discloses nucleotide sequences comprising, a regulatory region in operative association with xylanase secretion sequence and a gene of interest. The gene of interest encodes a protein selected from a pharmaceutical, nutraceutical, industrial, animal feed, food additive and an enzyme. Preferably, the gene of interest encodes a cellulase, hernicellulase, a lignin degrading enzyme, pectinase, protease, or peroxidase. The present invention also relates to vectors and hosts comprising these nucleic acid sequences, and to methods for the production of a protein of interest.

摘要翻译： 本发明涉及从淡水宿主中增加感兴趣的蛋白质的产生。 本发明公开了包含与木聚糖酶分泌序列有效结合的调节区和感兴趣的基因的核苷酸序列。 目的基因编码选自药物，营养品，工业，动物饲料，食品添加剂和酶的蛋白质。 优选地，感兴趣的基因编码纤维素酶，丝氨酸酶，木质素降解酶，果胶酶，蛋白酶或过氧化物酶。 本发明还涉及包含这些核酸序列的载体和宿主以及用于产生感兴趣的蛋白质的方法。

公开(公告)号：US10654235B2

公开(公告)日：2020-05-19

申请号：US14408068

申请日：2013-06-11

申请人： VOITH PATENT GMBH ,  IOGEN ENERGY CORPORATION

发明人： William Miller ,  Trevor Richardson ,  Stephen A. Rowland

IPC分类号： B30B9/12 ,  B30B9/18 ,  B30B9/14 ,  C02F11/125

摘要： The method includes the following steps: (i) feeding a slurry into the inlet of a press; (ii) removing liquid from the slurry as the slurry advances through a deliquefying section of the press, thereby producing a pressate stream; (iii) forming a plug of material as the slurry is advanced through a plug formation section of the press; (iv) advancing the plug of material to a discharge section of the press; and (v) removing additional liquid from the plug of material by operating the press so that the pressure in the discharge section of the press is greater than the pressure of the pressate stream by at least 2 psi. There is also described a method for controlling discharge consistency from a press, without provision of discharge restrictors, by varying a pressure differential between a discharge section of the press and a pressate stream.

公开(公告)号：US10407705B2

公开(公告)日：2019-09-10

申请号：US15788482

申请日：2017-10-19

申请人： Iogen Energy Corporation

发明人： Philippe J. Dufresne ,  Adam H. Colville ,  Barbara Fryzuk ,  Loreta Gudynaite-Savitch ,  Christopher M. D. Hindle ,  Boguslaw Ploch ,  John J. Tomashek

IPC分类号： C12N1/15 ,  C12N1/22 ,  C12N9/14 ,  C12N9/24 ,  C12P19/14 ,  C12N1/14 ,  C07K14/37 ,  C07K14/38 ,  C12P19/00 ,  C12N9/42 ,  C12P19/02

摘要： The present invention provides an isolated fungal cell that is capable of producing one or more biomass-degrading enzymes and that exhibits increased or decreased expression or copy number of a polynucleotide encoding a PtaB-like protein. Also provided is a fermentation processes for producing one or more biomass-degrading enzymes comprising a fungal cells exhibiting increased or decreased expression or copy number of a polynucleotide encoding a PtaB-like protein. The biomass-degrading enzymes produced by the isolate fungal cell and fermentation processes of the present invention may be used in a process to produce soluble sugars from biomass.

公开(公告)号：US10179971B2

公开(公告)日：2019-01-15

申请号：US15127693

申请日：2015-03-20

申请人： IOGEN ENERGY CORPORATION

发明人： Robert Griffin ,  Torbjorn van der Meulen ,  Harold Neumann ,  Robert Glenns

IPC分类号： C12P7/10 ,  C12P7/14 ,  C12P7/16 ,  D21C1/10 ,  C12P19/14 ,  D21C1/04 ,  D21C5/00 ,  C12P19/02 ,  C13K1/02 ,  D21C1/00

摘要： Provided herein is a process for producing an alcohol from a sugar cane derived cellulosic feedstock comprising: subjecting the sugar cane derived cellulosic feedstock comprising bagasse, leaves, tops, or any combination thereof, to silica removal selected from processes comprising sieving, screening, washing, cyclone separation or any combination thereof. The bagasse, leaves or tops, or any combination thereof, from which at least a portion of the silica has been removed is treated in one or more processing stages to produce sugar, wherein the undissolved solids content of a slurry during said processing stages reaches a weight percent of at least 15%. Thereafter the sugar is fermented with yeast or bacteria to produce the alcohol and the alcohol is concentrated and recovered.

公开(公告)号：US09279163B2

公开(公告)日：2016-03-08

申请号：US13593735

申请日：2012-08-24

申请人： Jacqueline Montalibet ,  Loreta Gudynaite-Savitch ,  Christopher Hill ,  Christopher D. Hindle ,  James A. Lavigne ,  Nabil Masri ,  Fuad Tahna ,  John J. Tomashek

发明人： Jacqueline Montalibet ,  Loreta Gudynaite-Savitch ,  Christopher Hill ,  Christopher D. Hindle ,  James A. Lavigne ,  Nabil Masri ,  Fuad Tahna ,  John J. Tomashek

IPC分类号： C12N9/42 ,  C12N15/80 ,  C12P19/14

CPC分类号： C12Y302/01091 ,  C12N9/2437 ,  C12N15/80 ,  C12P19/14

摘要： Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolyzing a cellulosic substrate with such cellulase enzyme mixtures.

摘要翻译： 提供的是分离的纤维二糖水解酶，其包含修饰的糖苷水解酶（GH）家族7催化结构域，GH家族7催化结构域和修饰的家族1碳水化合物结合模块（CBM），或修饰的家族7催化结构域和修饰的家族1 CBM两者。 这种分离的纤维二糖水解酶显示与SEQ ID NO：1的氨基酸1-436或SEQ ID NO：2的氨基酸1-438具有45％至约99.9％的氨基酸序列同一性，并且改善了对工艺底物的活性。 还提供了用于表达分离的纤维二糖水解酶的遗传构建体和遗传修饰的微生物，用于产生分离的纤维二糖水解酶的方法，包含分离的纤维二糖水解酶的纤维素酶混合物和用这种纤维素酶混合物水解纤维素底物的方法。

公开(公告)号：US08936929B2

公开(公告)日：2015-01-20

申请号：US14045278

申请日：2013-10-03

申请人： Iogen Energy Corporation

发明人： Jennifer Donaldson ,  Jan-Maarten A. Geertman ,  Glenn D. Munkvold ,  Gary Pigeau ,  Philippe J. Dufresne ,  Loreta Gudynaite-Savitch

IPC分类号： C12P7/10 ,  C12N9/00 ,  C12N9/42 ,  C12N1/00 ,  C07H21/04 ,  C12N15/81 ,  C12N15/01 ,  C12N15/52 ,  C12P7/08

CPC分类号： C12N15/81 ,  C12N15/01 ,  C12N15/52 ,  C12P7/08 ,  C12P7/10 ,  Y02E50/16

摘要： The present invention relates to novel xylose-fermenting yeast strains (for example, yeast of the genus Saccharomyces, e.g., S. cerevisiae) with an enhanced ability to ferment the xylose (and/or another pentose sugar) present in a lignocellulosic hydrolysate to a fermentation product(s) (for example, an alcohol (e.g., ethanol) or a sugar alcohol (e.g., xylitol)).

摘要翻译： 本发明涉及具有增强木质纤维素水解产物中存在的木糖（和/或其他戊糖）的能力的新型木糖发酵酵母菌株（例如酿酒酵母属酵母，例如酿酒酵母） 发酵产物（例如，醇（例如乙醇）或糖醇（例如木糖醇））。

公开(公告)号：US08882925B2

公开(公告)日：2014-11-11

申请号：US13522069

申请日：2011-02-08

申请人： Robert Griffin ,  Vladimir Pavlovic ,  Daphne Wahnon

发明人： Robert Griffin ,  Vladimir Pavlovic ,  Daphne Wahnon

IPC分类号： C13K13/00 ,  C08H7/00 ,  D21C9/00 ,  D21C5/00 ,  C13K1/02 ,  C08H8/00 ,  D21C9/08

CPC分类号： C13K1/02 ,  C08H6/00 ,  C08H8/00 ,  C12P2201/00 ,  D21C5/005 ,  D21C9/008 ,  D21C9/083 ,  Y02E50/16

摘要： A method for processing of a lignocellulosic feedstock is provided comprising exposing lignocellulosic feedstock to acid in a reactor to hydrolyze at least hemicellulose present in said lignocellulosic feedstock, wherein during said exposing, a scale deposit comprising lignin forms on the inner surface of said reactor; and treating the scale deposit with an alkali solution at a temperature between about 140° C. and about 250° C. so as to remove scale. Also provided is a method for reducing scale deposit that forms on process equipment during a stage of reacting a lignocellulosic feedstock with acid.

摘要翻译： 提供了一种用于处理木质纤维素原料的方法，包括在反应器中将木质纤维素原料暴露于酸中以至少水解所述木质纤维素原料中存在的半纤维素，其中在所述暴露期间，在所述反应器的内表面上包含木质素形式的垢沉积物; 并在约140℃至约250℃之间的温度下用碱溶液处理垢垢，以除去垢。 还提供了一种用于在使木质纤维素原料与酸反应的阶段期间减少在工艺设备上形成的垢沉积物的方法。