公开(公告)号：US20140113365A1

公开(公告)日：2014-04-24

申请号：US14114134

申请日：2012-04-26

申请人： Yusuke Nagai ,  Keiji Naruse ,  Ken Takahashi ,  Eijiro Tokuyama

发明人： Yusuke Nagai ,  Keiji Naruse ,  Ken Takahashi ,  Eijiro Tokuyama

IPC分类号： C12M1/00

CPC分类号： C12M23/26 ,  C12M23/04 ,  C12M23/34

摘要： A cell culture vessel supplies cells close to the center of gel and deeper down to the bottom with sufficient nutrients for attaining an enhanced survival rate of the cultivated cells. The cell culturing vessel comprises a culture tray capable of holding a liquid culture medium and a cell mixture container fixed to inner walls of the culture tray for retaining the cell mixture. Once the culture tray is filled with the liquid culture medium, the cell mixture retained in the cell mixture container has its opposite outer parts exposed to the liquid culture medium.

摘要翻译： 细胞培养容器提供靠近凝胶中心的细胞，更深层次地向底部提供足够的营养物质，以达到提高培养细胞的存活率。 细胞培养容器包括能够保持液体培养基的培养托盘和固定在培养托盘的内壁上的细胞混合物容器，用于保持细胞混合物。 一旦培养皿填充有液体培养基，保留在细胞混合容器中的细胞混合物具有暴露于液体培养基的相对的外部部分。

公开(公告)号：US20140055208A1

公开(公告)日：2014-02-27

申请号：US13972172

申请日：2013-08-21

申请人： NATIONAL UNIVERSITY CORPORATION OKAYAMA UNIVERSITY ,  NEC TOKIN CORPORATION

发明人： Koichi KONDO ,  Naoharu YAMAMOTO ,  Yoshitaka TOYOTA ,  Kengo IOKIBE ,  Farhan Zaheed MAHMOOD

IPC分类号： H04B3/28 ,  H03H7/01

CPC分类号： H01P7/10 ,  H01P1/2005 ,  H01P1/20381 ,  H01P7/082

摘要： A resonator is connected to a first plane which is one of a power plane and a ground plane, wherein the power plane and the ground plane are apart from each other in an up-down direction. The resonator comprises a connecting portion and a body portion. The connecting portion is connected to the first plane. The connecting portion extends in the up-down direction beyond a second plane, which is a remaining one of the power plane and the ground plane, while not being in electrical contact with the second plane. The body portion is connected to the connecting portion while not being in contact with the second plane. The body portion is arranged so that the second plane is located between the body portion and the first plane in the up-down direction.

摘要翻译： 谐振器连接到作为电力平面和接地平面之一的第一平面，其中电力平面和接地平面在上下方向上彼此分开。 谐振器包括连接部分和主体部分。 连接部分连接到第一平面。 连接部分在上下方向上延伸超过作为动力平面和接地平面中的剩余部分的第二平面，而不与第二平面电接触。 主体部分连接到连接部分而不与第二平面接触。 主体部分被布置成使得第二平面在上下方向上位于主体部分和第一平面之间。

公开(公告)号：US08608927B2

公开(公告)日：2013-12-17

申请号：US13145943

申请日：2010-01-28

申请人： Keiji Tsukada

发明人： Keiji Tsukada

IPC分类号： G01N27/333

CPC分类号： G01N27/333

摘要： Provided is an ion sensor with which the concentration of ions in a solution can be determined without any reference electrode. The ion sensor is equipped with: a first electrode plate; a second electrode plate which has been disposed opposite the first electrode plate and has one or multiple openings; an ion-sensitive film continuously formed so that the film is interposed between the first electrode plate and the second electrode plate, blocks up one side of each opening of the second electrode plate, and extends from the end of that side of the opening to the outer surface of the second electrode plate through the inner wall surface of the opening; and a sensor support which supports the second electrode plate so that when the ion concentration of a test solution to be examined is determined, the ion-sensitive film comes into contact with the test solution only in the portions thereof that have been formed in each opening and on the outer surface of the second electrode plate. The ion sensor is configured so that the thickness of the ion-sensitive film interposed between the first electrode plate and the second electrode plate is different from the thickness of the ion-sensitive film formed on the outer surface of the second electrode plate. A difference in potential between the first electrode plate and the second electrode plate is measured.

摘要翻译： 提供了一种离子传感器，可以在没有任何参考电极的情况下确定溶液中的离子浓度。 离子传感器配备有：第一电极板; 第二电极板，其与第一电极板相对设置并具有一个或多个开口; 连续地形成离子敏感膜，使得膜介于第一电极板和第二电极板之间，阻挡第二电极板的每个开口的一侧，并从开口的该侧的端部延伸到 第二电极板的外表面通过开口的内壁表面; 以及传感器支撑体，其支撑所述第二电极板，使得当确定所述待检测溶液的离子浓度时，所述离子敏感膜仅在其每个开口中形成的部分中与所述测试溶液接触 并且在第二电极板的外表面上。 离子传感器被配置为使得介于第一电极板和第二电极板之间的离子敏感膜的厚度与形成在第二电极板的外表面上的离子敏感膜的厚度不同。 测量第一电极板和第二电极板之间的电位差。

公开(公告)号：US20130171698A1

公开(公告)日：2013-07-04

申请号：US13816826

申请日：2011-08-11

申请人： Kenji Watanabe ,  Hisao Moriya

发明人： Kenji Watanabe ,  Hisao Moriya

IPC分类号： C12P19/34

CPC分类号： C12P19/34 ,  C12N15/52 ,  C12N15/80 ,  C12N15/815 ,  C12P17/06 ,  C12P21/02

摘要： The present invention relates to a method of removing an intron contained in a gene from a eukaryotic gene, and linking only the exon sequences to prepare an expression vector comprising the linked sequences. Specifically, the invention relates to a method of preparing an expression vector containing linked exon sequences comprising amplifying exon sequences by PCR as one or more fragments from a giant fungal gene containing an intron, and linking the fragments together with a restriction enzyme-treated vector using the gap repair cloning method; a method of preparing an expression vector containing a full-length cDNA sequence by synthesizing and linking cDNA fragments from a fungal giant gene; a transformant having introduced therein an expression vector prepared by the method; a protein produced by the transformant; and a method of preparing a compound produced by the protein using the expression vector.

摘要翻译： 本发明涉及从真核基因中除去包含在基因中的内含子的方法，并且仅连接外显子序列以制备包含连接序列的表达载体。 具体地，本发明涉及一种制备含有连锁外显子序列的表达载体的方法，其包括通过PCR扩增外显子序列作为来自含有内含子的巨型真菌基因的一个或多个片段，并将所述片段与限制酶处理的载体连接，使用 间隙修复克隆方法; 通过合成和连接来自真菌巨基因的cDNA片段制备含有全长cDNA序列的表达载体的方法; 在其中引入了通过该方法制备的表达载体的转化体; 由转化体产生的蛋白质; 以及使用表达载体制备由蛋白质产生的化合物的方法。

公开(公告)号：US08477934B2

公开(公告)日：2013-07-02

申请号：US13264897

申请日：2010-04-21

申请人： Yasuyuki Nogami ,  Yumi Sakemi ,  Hiroaki Nasu ,  Yoshitaka Morikawa

发明人： Yasuyuki Nogami ,  Yumi Sakemi ,  Hiroaki Nasu ,  Yoshitaka Morikawa

IPC分类号： H04K1/00

CPC分类号： H04L9/3252 ,  G06F7/725 ,  H04L9/3073

摘要： There is provided a pairing computation device provided with a CPU which computes pairing e(S,Q) with SεG1, QεG2, χ being a given integer variable, and F being a rational function calculated using Miller's algorithm with respect to multi-pairing (MMA). An order r, a trace t of the Frobenius endomorphism φp are specified preliminarily using the integer variable χ according to an embedding degree k. The CPU performs pairing computation by means of: an input unit which inputs the integer variable χ, the rational point S, and the rational point Q into respective predetermined registers; a computation unit which computes F; a computation unit which computes a value at a rational point Q(xQ, yQ) of a straight line passing through given rational points; a computation unit which computes f′ χ,S(Q) using the aforementioned F and the value; and a computation unit which computes the pairing e(S,Q) using aforementioned f′ χ,S(Q) as [F83].[F83] e(S,Q)=f′χ,S(Q)(pk−1)/r.

摘要翻译： 提供了一种配对计算装置，其具有CPU，其使用SepsilonG1，QepsilonG2，chi作为给定的整数变量来计算配对e（S，Q），F是使用关于多重配对（MMA）的米勒算法计算的有理函数 ）。 根据嵌入度k，使用整数变量chi预先规定Frobenius内同型phip的阶数r，trace t。 CPU通过以下方式执行配对计算：将整数变量chi，有理点S和有理点Q输入到各个预定寄存器中的输入单元; 计算F的计算单元; 计算通过给定理性点的直线的有理点Q（xQ，yQ）的值的计算单元; 计算单元，其使用前述F计算f'chi，S（Q）和该值; 以及使用上述f'chi，S（Q）为[F83]来计算配对e（S，Q）的计算单元。 [F83] e（S，Q）= f'chi，S（Q）（pk-1）/ r。

公开(公告)号：US08388934B2

公开(公告)日：2013-03-05

申请号：US13137295

申请日：2011-08-04

申请人： Yoshirou Kawahara

发明人： Yoshirou Kawahara

IPC分类号： A61K49/00 ,  A61P1/00

CPC分类号： A61K49/006 ,  A61B1/00 ,  A61B1/273 ,  A61B5/0059 ,  A61B5/42

摘要： A method of conducting an endoscopic observation of an inner wall of a gastrointestine by applying a diagnostic agent to the inner wall of the gastrointestine, the diagnostic agent being an acidic aqueous solution of pH 1 to 5 containing a colorant, and conducting the endoscopic observation of the inner wall. The acidic aqueous solution can contain at least one acid selected from a carboxylic acid, hydrochloric acid, sulfuric acid and phosphoric acid. By applying the diagnostic agent to the inner wall of the gastrointestine in the endoscopic observation, it is possible to clearly observe a lesion which is difficult to be determined. In particular, the method can be used for observing lesions having cancer cells in the stomach or the Barrett's esophagus.

摘要翻译： 通过将诊断剂施用到胃肠道的内壁进行胃肠内壁的内窥镜观察的方法，所述诊断剂是含有着色剂的pH1〜5的酸性水溶液，进行内窥镜观察 内墙。 酸性水溶液可含有至少一种选自羧酸，盐酸，硫酸和磷酸的酸。 通过在内窥镜观察中将诊断剂施用于胃肠道的内壁，可以清楚地观察难以确定的病变。 特别地，该方法可用于观察在胃或Barrett食管中具有癌细胞的病变。

公开(公告)号：US08238550B2

公开(公告)日：2012-08-07

申请号：US12740587

申请日：2008-10-29

申请人： Masataka Akane ,  Yasuyuki Nogami ,  Yoshitaka Morikawa

发明人： Masataka Akane ,  Yasuyuki Nogami ,  Yoshitaka Morikawa

IPC分类号： G06F7/04 ,  G06F17/30 ,  H04N7/16

CPC分类号： H04L9/3073 ,  G06F7/725 ,  H04L9/3255 ,  H04L2209/046

摘要： There is provided a pairing computation device, a pairing computation method and a recording medium recording a pairing computation program all enabling a pairing computation at high speed. In the case where the Ate pairing e(Q,P) is defined as in equation (1) and k is any one of even, a multiple of 3, a multiple of 4, and a multiple of 6, arithmetic operations of a rational function which is required to derive the Miller function fs,Q(P) are performed on the proper subfield specified by a twist curve using a quadratic and cubic non-residue v which becomes 1 when an exponentiation of this fs,Q(P) to the power of (qk−1)/r is performed.

摘要翻译： 提供了配对计算装置，配对计算方法和记录配对计算程序的记录介质，全部能够高速配对计算。 在Ate对e（Q，P）如等式（1）中定义的情况下，k是偶数，3的倍数，4的倍数和6的倍数，理性的算术运算 在使用二次和三次非残余v的情况下，在通过扭曲曲线指定的适当子场上执行米勒函数fs，Q（P）所需的函数，当该fs，Q（P）与 执行（qk-1）/ r的功率。

公开(公告)号：US20120174257A1

公开(公告)日：2012-07-05

申请号：US13394569

申请日：2010-09-03

申请人： Jian Feng Ma

发明人： Jian Feng Ma

IPC分类号： A01H1/06 ,  C12N15/29 ,  A01H5/00

CPC分类号： C12N15/8271 ,  C07K14/415 ,  C12N15/8259

摘要： Identifying a gene responsible for the accumulation of cadmium in plants, the present invention provides a method for using such a gene. A polynucleotide encoding a polypeptide consisting of the amino acid sequence of SEQ ID NO: 1, a polynucleotide encoding a polypeptide in which one or several amino acids are substituted, deleted, and/or added in the amino acid sequence of SEQ ID NO: 1 and which has activity to change the localization of cadmium accumulation, a polynucleotide encoding a polypeptide consisting of the amino acid sequence of SEQ ID NO: 2, or a polynucleotide encoding a polypeptide in which one or several amino acids are substituted, deleted, and/or added in the amino acid sequence of SEQ ID NO: 2 and which has activity to change the localization of cadmium accumulation is introduced into a plant such that the polynucleotide is able to be expressed.

摘要翻译： 鉴定负责植物中镉累积的基因，本发明提供了使用这种基因的方法。 编码由SEQ ID NO：1的氨基酸序列组成的多肽的多核苷酸，编码多肽的多核苷酸，其中一个或多个氨基酸被取代，缺失和/或添加到SEQ ID NO：1的氨基酸序列中 并且其具有改变镉积累的定位的活性，编码由SEQ ID NO：2的氨基酸序列组成的多肽的多核苷酸或编码多肽的多核苷酸，其中一个或几个氨基酸被取代，缺失和/ 或添加在SEQ ID NO：2的氨基酸序列中，并且其具有改变镉积累的定位的活性被引入植物中，使得多核苷酸能够被表达。

公开(公告)号：US20110179471A1

公开(公告)日：2011-07-21

申请号：US13060520

申请日：2009-08-28

申请人： Yasuyuki Nogami ,  Masataka Akane ,  Yumi Sakemi ,  Yoshitaka Morikawa

发明人： Yasuyuki Nogami ,  Masataka Akane ,  Yumi Sakemi ,  Yoshitaka Morikawa

IPC分类号： H04L9/32 ,  G06F21/00

CPC分类号： H04L9/3073

摘要： Provided are a pairing computation device, a pairing computation method, and a pairing computation program all of which enable fast pairing computation. The pairing computation device includes, where: E is an additive group of rational points on a pairable elliptic curve defined over Fpk with an embedding degree of k, and the curve equation is given by y2=x3+ax+b where a∈Fp and b∈Fp, E[r] is a set of rational points of a prime order r, φp is Frobenius endomorphism, and the order r and a trace t of the Frobenius endomorphism φp are used as functions of an integer variable χ: computation means for computing a rational function fχ,Q(S); computation means for computing a value of a rational point S(xs, ys) on a line passing through a predetermined rational point; computation means for computing a rational function f′χ,Q(S) using computation results of these computation means; and computation means for performing pairing computation by using the rational function f′χ,Q(S) as Formula (1): e(Q,S)=ƒ′χ,Q(S)(p−1)/r   (1).

摘要翻译： 提供了配对计算装置，配对计算方法和配对计算程序，所有这些都能够进行快速配对计算。 配对计算装置包括：其中E是在嵌入度为k的Fpk上定义的配对椭圆曲线上的有理点的加性组，曲线方程由y2 = x3 + ax + b给出，其中a∈Fp和 b∈Fp，E [r]是素数阶r的理论点集合，p是Frobenius内同构，Frobenius内同形函数和p的p的阶数r和trace t用作整数变量的函数 χ：用于计算有理函数fχ，Q（S）的计算装置; 用于计算通过预定有理点的线上的有理点S（xs，ys）的值的计算装置; 计算装置，用于使用这些计算装置的计算结果计算有理函数f'χ，Q（S）; 通过使用有理函数f'χ，Q（S）作为公式（1）进行配对计算的计算装置：e（Q，S）=ƒ'χ，Q（S）（p-1）/ r ）。

公开(公告)号：US20110065117A1

公开(公告)日：2011-03-17

申请号：US12933892

申请日：2009-02-24

申请人： Noriaki Tanaka ,  Junji Matsuoka ,  Takuya Fukazawa

发明人： Noriaki Tanaka ,  Junji Matsuoka ,  Takuya Fukazawa

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N15/63

CPC分类号： C12N15/85 ,  A61K38/00 ,  A61K48/0058 ,  C07K14/82 ,  C12N2710/10343 ,  C12N2830/008

摘要： Provided is a promoter showing transcriptional activity in a mesothelioma-specific manner and showing low transcriptional activity in other kinds of cancer cells and normal cells including mesothelium. Also provided are applications of the promoter, and more specifically, a gene therapy vector and a therapeutic agent for mesothelioma each including the promoter. The promoter includes a CRI1 gene-derived promoter, which is one kind of mesothelioma markers. The use of a vector including a cell death-inducing gene or a cell lysis-inducing gene as a transgene and carrying the CRI1 gene-derived promoter upstream of the transgene can induce a cell death or cell lysis action in a mesothelioma-specific manner. That is, the gene therapy vector and the therapeutic agent for mesothelioma each include a virus vector carrying the CRI1 gene-derived promoter.

摘要翻译： 提供了以间皮瘤特异性方式显示转录活性的启动子，并且在其他种类的癌细胞和包括间皮的正常细胞中显示低转录活性。 还提供了启动子的应用，更具体地，涉及包含启动子的间皮瘤的基因治疗载体和治疗剂。 启动子包括一种CRI1基因来源的启动子，其是一种间皮瘤标记物。 使用包含细胞死亡诱导基因或细胞裂解诱导基因作为转基因并在转基因上游携带CRI1基因的启动子的载体可以以间皮瘤特异性方式诱导细胞死亡或细胞裂解作用。 也就是说，基因治疗载体和间皮瘤的治疗剂各自包含携带CRI1基因来源的启动子的病毒载体。