BIOLOGICAL INDICATOR
    99.
    发明申请

    公开(公告)号:US20160102335A1

    公开(公告)日:2016-04-14

    申请号:US14512708

    申请日:2014-10-13

    Abstract: This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed.

    Combined sterilization indicator incubator and reader system
    100.
    发明授权
    Combined sterilization indicator incubator and reader system 有权
    联合灭菌指示器孵化器和阅读器系统

    公开(公告)号:US09303283B2

    公开(公告)日:2016-04-05

    申请号:US14090116

    申请日:2013-11-26

    CPC classification number: C12Q1/22 A61L2/28

    Abstract: A combined sterilization indicator incubator and reader system, including a sterilization indicator vial containing one of a variety of selected biological indicators and a liquid, at least two incubator blocks, each incubator block independently operable to incubate the sterilization indicator vial at a plurality of independently selectable temperatures, each of the incubator blocks including at least one heating element operable to heat the incubator block to any one of the plurality of independently selectable temperatures; a light source, a photodetector and a control system configured to operate the combined system to determine the efficacy of a variety of sterilization processes. The system calculates and compares a slope to a predetermined threshold slope for the biological indicator, and provides output based only on the comparison of the calculated slope to the predetermined threshold slope without first determining either a baseline or a minimum value of the output from the photodetector.

    Abstract translation: 一种组合的灭菌指示器培养箱和读取器系统,包括含有多种选择的生物指示剂中的一种的灭菌指示剂小瓶和液体的至少两个培养箱块,每个培养箱块可独立操作以将灭菌指示剂小瓶以多个可独立选择的温度 每个孵化器块包括至少一个加热元件,其可操作以将培养箱块加热到多个可独立选择的温度中的任何一个; 光源,光电检测器和控制系统,其被配置为操作组合系统以确定各种灭菌过程的功效。 该系统计算并比较生物学指示器的斜率与预定阈值斜率,并且仅基于所计算的斜率与预定阈值斜率的比较来提供输出,而不首先确定来自光电探测器的输出的基线或最小值 。

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