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公开(公告)号：US20170137872A1
公开(公告)日：2017-05-18
申请号：US15350441
申请日：2016-11-14
Applicant: Keygene N.V.
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
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公开(公告)号：US20170081718A1
公开(公告)日：2017-03-23
申请号：US15366417
申请日：2016-12-01
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia VAN EIJK , Anker Preben SØRENSEN , Marco Gerardus Maria VAN SCHRIEK
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6876 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2565/301 , C12Q2535/138 , C12Q2535/122 , C12Q2527/107
Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
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93.发明申请HIGH THROUGHPUT SCREENING OF POPULATIONS CARRYING NATURALLY OCCURRING MUTATIONS 审中-公开人道主义进行自然的重大突破的高速度筛选公开(公告)号：US20170022560A1
公开(公告)日：2017-01-26
申请号：US15288253
申请日：2016-10-07
Applicant: Keygene N.V.
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q1/6827 , C12Q1/6846 , C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , C12Q1/6869 , C12Q2600/13 , G06F19/22 , C12Q2525/155 , C12Q2563/179 , C12Q2563/155 , C12Q2537/143
Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.
Abstract translation: 公开了有效的方法用于在诱变的群体成员中基因突变的高通量鉴定。 所述方法包括DNA分离，合并，扩增，文库的产生，文库的高通量测序，优选通过合成序列测序技术，鉴定突变和携带突变和识别突变的群体成员的鉴定。
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公开(公告)号：US20170016020A1
公开(公告)日：2017-01-19
申请号：US15280081
申请日：2016-09-29
Applicant: Enza Zaden Beheer B.V. , Keygene N.V.
Inventor： Paul Johan Diergaarde , Leonora Johanna Gertruda van Enckevort , Karin Ingeborg Posthuma , Marinus Willem Prins
IPC: C12N15/82 , C07K14/415
CPC classification number: C12N15/8282 , C07K14/415
Abstract: The present invention relates to powdery mildew resistance providing genes of the Cucumis family, and especially Cucumis sativus, wherein said resistance is provided by impairment of the present genes. Further, the present invention relates plants comprising the present impaired resistance conferring genes and seeds, embryos or other propagation material thereof. Especially, the present invention relates to powdery mildew resistance conferring genes, wherein the amino acid sequence encoded by said resistance conferring gene is selected from the group consisting of SEQ ID No. 2, SEQ ID No. 4, SEQ ID No. 6, SEQ ID No. 8, SEQ ID No. 10, SEQ ID No. 12, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 18, SEQ ID No. 20 and SEQ ID No. 22, and amino acid sequences with more than 70% identity, preferably more than 80% identity, more preferably more than 90% identity, and most preferably more than 95% identity.
Abstract translation: 本发明涉及提供黄瓜（Cucumis）家族特别是黄瓜（Cucumis sativus）的基因的白粉病抗性，其中所述抗性是通过本发明基因的损伤来提供的。 此外，本发明涉及包含赋予基因和种子的本发明的抗性抗性的植物，胚胎或其它繁殖材料。 特别地，本发明涉及赋予白粉病抗性的基因，其中由所述抗性赋予基因编码的氨基酸序列选自SEQ ID No.2，SEQ ID No.4，SEQ ID No.6，SEQ ID No.8，SEQ ID No.10，SEQ ID No.12，SEQ ID No.14，SEQ ID No.16，SEQ ID No.18，SEQ ID No.20和SEQ ID No.22，以及氨基酸 具有超过70％同一性，优选大于80％同一性，更优选大于90％同一性，最优选大于95％同一性的序列。
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公开(公告)号：US20160145689A1
公开(公告)日：2016-05-26
申请号：US15009304
申请日：2016-01-28
Applicant: Keygene N.V.
IPC: C12Q1/68
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
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Patent Agency Ranking
公开(公告)号：US20160017364A1
公开(公告)日：2016-01-21
申请号：US14809875
申请日：2015-07-27
Applicant: Keygene N.V.
IPC: C12N15/82
CPC classification number: C12N15/8286 , A01H1/04 , C12N9/88 , C12N15/8239 , C12N15/8243 , C12P5/007 , C12Y402/00 , C12Y402/03053 , C12Y402/03054 , C12Y402/03082 , Y02A40/162
Abstract: The disclosure provides an isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising said nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.
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公开(公告)号：US20150344946A1
公开(公告)日：2015-12-03
申请号：US14699891
申请日：2015-04-29
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia VAN EIJK , Anker Preben Sørensen , Marco Gerardus Maria Van Schriek
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6876 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2565/301 , C12Q2535/138 , C12Q2535/122 , C12Q2527/107
Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
Abstract translation: 本发明涉及一种用于一种或多种样品中一种或多种遗传标记物的高通量发现，检测和基因分型的方法，其包括DNA的限制性内切核酸酶消化，衔接子连接，任选的预扩增，选择性扩增，合并 的扩增产物，对文库以足够的冗余进行测序，聚类，然后鉴定文库内和/或文库之间的遗传标记和确定遗传标记的（共）优势基因型。
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公开(公告)号：US20150284789A1
公开(公告)日：2015-10-08
申请号：US14742549
申请日：2015-06-17
Applicant: Keygene N.V.
Inventor： René Cornelis Josephus HOGERS
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q1/6869 , C12Q1/6855 , C12Q2521/501 , C12Q2525/161 , C12Q2525/191 , C12Q2525/197 , C12Q2525/307 , C12Q2531/125 , C12Q2535/122 , C12Q2537/159 , C12Q2563/179
Abstract: The method of the present invention now provides a technique for generating sequence information from nucleic acid samples based on knowledge from part(s) of the nucleotide sequence. The knowledge of the partial sequence may include knowledge about the presence of restriction sites. The knowledge of the partial sequence can be used to generate adaptor ligated or nucleotide-elongated fragments. From the combination of information on the ligated adaptor and the Known Nucleotide Sequence Section, probes can be designed. The probes can be used in the provision of circularised fragments that can be sequenced. Combining the known and determined sequences adds sequence information to the already existing sequence information and complements the available genomic sequence information.
Abstract translation: 本发明的方法现在提供了一种基于核苷酸序列的一部分的知识从核酸样品产生序列信息的技术。 部分序列的知识可以包括关于限制性位点的存在的知识。 部分序列的知识可用于产生衔接子连接或核苷酸延伸的片段。 从连接适配器和已知核苷酸序列部分的信息的组合可以设计探针。 探针可用于提供可排序的循环碎片。 结合已知和确定的序列将序列信息添加到已经存在的序列信息中并补充可用的基因组序列信息。
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99.发明授权公开(公告)号：US09023768B2
公开(公告)日：2015-05-05
申请号：US14253806
申请日：2014-04-15
Applicant: Keygene N.V.
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
Abstract translation: 本发明涉及通过在两个或多个样品上进行复杂性降低以产生两个或更多个文库，测序至少部分文库，对齐所鉴定的序列并确定任何推定的单核苷酸的方法，用于高通量鉴定单核苷酸多态性的方法 多态性，确认任何推定的单核苷酸多态性，产生用于确认的单核苷酸多态性的检测探针，将测试样品命名为相同的复杂性降低以提供测试文库并筛选测试文库以存在或不存在单核苷酸多态性 检测探头。
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公开(公告)号：US08815512B2
公开(公告)日：2014-08-26
申请号：US13666385
申请日：2012-11-01
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia Van Eijk , Anker Preben Sørensen , Marco Geradus Maria Van Schriek
CPC classification number: C12Q1/6874 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6876 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2565/301 , C12Q2535/138 , C12Q2535/122 , C12Q2527/107
Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
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