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公开(公告)号：US07329527B2
公开(公告)日：2008-02-12
申请号：US11014432
申请日：2004-12-16
申请人： David A. Estell
发明人： David A. Estell
摘要： The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
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公开(公告)号：US07329526B2
公开(公告)日：2008-02-12
申请号：US11014339
申请日：2004-12-16
申请人： David A. Estell
发明人： David A. Estell
摘要： The present invention relates to the identification of novel serine proteases in Gram-positive microorganisms. The present invention provides the nuclei acid and amino acid sequences for the Bacillus subtilis serine proteases SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding SP1, SP2, SP3, SP4 and SP5. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a serine protease of the present invention.
摘要翻译： 本发明涉及革兰氏阳性微生物中新型丝氨酸蛋白酶的鉴定。 本发明提供枯草芽孢杆菌丝氨酸蛋白酶SP1，SP2，SP3，SP4和SP5的核酸和氨基酸序列。 本发明还提供具有编码SP1，SP2，SP3，SP4和SP5的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的丝氨酸蛋白酶的清洁组合物。
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公开(公告)号：US07098021B2
公开(公告)日：2006-08-29
申请号：US11014361
申请日：2004-12-16
申请人： David A. Estell
发明人： David A. Estell
摘要： The present invention relates to the identification of a novel metalloproteases in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
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公开(公告)号：US07078372B2
公开(公告)日：2006-07-18
申请号：US10925821
申请日：2004-08-24
申请人： David A. Estell
发明人： David A. Estell
CPC分类号： C12N9/54 , A23K20/189 , C11D3/386 , D06M16/003
摘要： The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
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公开(公告)号：US07070986B2
公开(公告)日：2006-07-04
申请号：US10926197
申请日：2004-08-24
申请人： David A. Estell
发明人： David A. Estell
CPC分类号： C12N9/54 , A23K20/189 , C11D3/386 , D06M16/003
摘要： The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
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公开(公告)号：US07070819B2
公开(公告)日：2006-07-04
申请号：US10925652
申请日：2004-08-24
申请人： David A. Estell
发明人： David A. Estell
CPC分类号： C12N9/54 , A23K20/189 , C11D3/386 , D06M16/003
摘要： The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides hot cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.
摘要翻译： 本发明涉及革兰氏阳性微生物中新型金属蛋白酶的鉴定。 本发明提供了金属蛋白酶的核酸和氨基酸序列。 本发明还提供具有编码金属蛋白酶的基因的部分或全部的突变或缺失的热细胞。 本发明提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了用于处理包括本发明的金属蛋白酶的织物的清洁组合物，动物饲料和组合物。
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公开(公告)号：US06762039B2
公开(公告)日：2004-07-13
申请号：US09462846
申请日：2000-01-13
申请人： David A. Estell
发明人： David A. Estell
IPC分类号： C12P2104
摘要： The present invention relates to the identification of novel cysteine proteases in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the Bacillus subtilis cysteine proteases CP1, CP2 and CP3. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding CP1, CP2 or CP3. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides a cleaning composition comprising a cysteine protease of the present invention.
摘要翻译： 本发明涉及革兰氏阳性微生物中新型半胱氨酸蛋白酶的鉴定。 本发明提供枯草芽孢杆菌半胱氨酸蛋白酶CP1，CP2和CP3的核酸和氨基酸序列。 本发明还提供具有编码CP1，CP2或CP3的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的半胱氨酸蛋白酶的清洁组合物。
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公开(公告)号：US06489108B1
公开(公告)日：2002-12-03
申请号：US09555000
申请日：2000-05-23
申请人： David A. Estell
发明人： David A. Estell
IPC分类号： C12N1557
摘要： The present invention relates to the identification of novel metalloproteases in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metallprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metallprotease of the present invention.
摘要翻译： 本发明涉及革兰氏阳性微生物中新型金属蛋白酶的鉴定。 本发明提供了金属蛋白酶的核酸和氨基酸序列。 本发明还提供具有编码金属蛋白酶的基因的部分或全部的突变或缺失的宿主细胞。 本发明提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了用于处理包括本发明的金属蛋白酶的织物的清洁组合物，动物饲料和组合物。
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109.发明授权Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins 有权人类具有较低过敏反应的突变蛋白质以及用于构建，鉴定和产生此类蛋白质的方法公开(公告)号：US06218165B1
公开(公告)日：2001-04-17
申请号：US09255502
申请日：1999-02-23
申请人： David A. Estell , Fiona A. Harding
发明人： David A. Estell , Fiona A. Harding
IPC分类号： C12N954
CPC分类号： G01N33/6878 , C12N9/54 , G01N33/505
摘要： The present invention relates to a novel improved protein mutant which produces low allergenic response in humans compared to the parent of that mutant. Specifically, the present invention comprises neutralizing or reducing the ability of T-cells to recognize epitopes and thus prevent sensitization of an individual to the protein.
摘要翻译： 本发明涉及与该突变体的亲本相比，在人类中产生低变应原反应的新型改良蛋白质突变体。 具体地，本发明包括中和或降低T细胞识别表位的能力，从而防止个体对蛋白质的致敏。
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公开(公告)号：US5346823A
公开(公告)日：1994-09-13
申请号：US36592
申请日：1993-03-24
申请人： David A. Estell , James A. Wells , Richard R. Bott
发明人： David A. Estell , James A. Wells , Richard R. Bott
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： There are provided methods for making a mutant Bacillus subtilisin having altered oxidative stability, the methods comprising obtaining DNA fragment consisting of a region coding for a Bacillus subtilisin, and introducing a mutation into said DNA fragment such that the mutation is introduced in a region encoding a methionine, tryptophan, cysteine or lysine, sensitive to oxidation, such that upon expression of the mutant subtilisin one or more codon regions encoding for methionine, tryptophan, cysteine or lysine is replaced with an amino acid other than methionine, tryptophan, cysteine or lysine, preferably alanine or serine.
摘要翻译： 提供了制备具有改变的氧化稳定性的突变型枯草杆菌蛋白酶的方法，所述方法包括获得由编码枯草杆菌蛋白酶的区域组成的DNA片段，并将突变引入所述DNA片段，使得突变引入到编码 敏感的蛋氨酸，色氨酸，半胱氨酸或赖氨酸，使得突变型枯草杆菌蛋白酶表达后，编码甲硫氨酸，色氨酸，半胱氨酸或赖氨酸的一个或多个密码子区域被甲硫氨酸，色氨酸，半胱氨酸或赖氨酸以外的氨基酸替代， 优选丙氨酸或丝氨酸。
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