公开(公告)号：US20230235411A1

公开(公告)日：2023-07-27

申请号：US17282633

申请日：2019-10-04

申请人： First Light Diagnostics, Inc.

发明人： Don Straus

IPC分类号： C12Q1/689 ,  C12Q1/6841 ,  C12Q1/20

CPC分类号： C12Q1/689 ,  C12Q1/6841 ,  C12Q1/20

摘要： The invention provides systems and methods for rapid automated identification of microbes and antimicrobial susceptibility testing (AST) directly from a patient specimen, without specimen preparation. Specimens are loaded into an analytical cartridge for processing. Analytical cartridges are preloaded with species-specific labels that are used to identify and enumerate microbes in the specimen. Instruments, such as analyzers can be used to interact with analytical cartridges to carry out methods of the invention all within the cartridge.

公开(公告)号：US20230134003A1

公开(公告)日：2023-05-04

申请号：US17767730

申请日：2020-10-09

申请人： Lightcast Discovery Ltd

发明人： Cameron FRAYLING ,  Thomas Henry ISAAC ,  Andreas Michael WAEBER

IPC分类号： C12Q1/20 ,  B01L3/00

摘要： A method for determining an interaction between a medicament and a cell type comprising an array of first microdroplets, each containing a cell type derived from a biological sample, an array of second microdroplets, each containing one or more medicaments at one or more predetermined concentrations, merging the array of first microdroplets and the array of second microdroplets to form an array of merged microdroplets, and monitoring the characteristics of one or more cells in the merged microdroplets using an optical detection system configured to detect an interaction between a cell type and a medicament.

公开(公告)号：US20220348979A1

公开(公告)日：2022-11-03

申请号：US17760740

申请日：2020-10-06

申请人： QuantaMatrix Inc.

发明人： Gi Yoon LEE ,  Tae Geun LIM ,  Dong Young KIM

IPC分类号： C12Q1/20 ,  B01L3/00

摘要： Provided is a multi-well-based rapid cell culture test device designed such that fluid films with a uniform thickness are formed. The rapid cell culture test device has an array structure of a plurality of aligned well units, each of which includes a first sub-well in which a first fluid is accommodated and a barrier structure surrounding the first sub-well to define the area of the first sub-well. A capillary channel recessed from the bottom surface of the first sub-well while traversing the bottom surface is formed to accommodate the first fluid and the barrier structure is divided into a barrier portion A located adjacent to one end of the capillary channel and a barrier portion B located adjacent to the other end of the capillary channel. The barrier portion A is greater in height than the barrier portion B such that when the barrier portion A is wetted with the first fluid introduced into the first sub-well along its inner wall, a change in the level of the first fluid at the other end of the capillary channel depending on the amount of the first fluid dispensed is minimized.

公开(公告)号：US20220235392A1

公开(公告)日：2022-07-28

申请号：US17615256

申请日：2019-09-20

申请人： Ian Andrew Lewis ,  Thomas Rydzak

发明人： Ian Andrew Lewis ,  Thomas Rydzak

IPC分类号： C12Q1/04 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/20 ,  C12N1/20

摘要： Methods and systems are for identifying the cell type of an unknown microorganism. The device includes: an apparatus for culturing unknown organism(s), a diagnostic data acquisition tool and a computer program. The method includes: incubation of the sample with a growth medium (with or without toxins), and an analysis of the metabolites detected in the sample. The computer system compares the results collected from the device to reference metabolite profiles.

公开(公告)号：US20220213523A1

公开(公告)日：2022-07-07

申请号：US17698518

申请日：2022-03-18

申请人： BD KIESTRA B.V.

发明人： Raphael Rodolphe Marcelpoil

IPC分类号： C12Q1/18 ,  C12Q1/20 ,  G06T7/11 ,  G01N21/17 ,  G06T7/00 ,  G06T7/90 ,  G06V20/69

摘要： An imaging system and method provides automated microbial growth detection for antibiotic sensitivity testing. A processing system having an image sensor for capturing images of an inoculated culture plate having antibiotic disks disposed on the culture media captures images of the plate at separate times (e.g., first and second images). The system generates pixel characteristic data for pixels of the second image from a comparison of the first image and second image. The pixel characteristic data may be indicative of plate growth. The system may access growth modeling data concerning the antibiotic disk(s) and generate simulated image data with a growth model function. The growth model function uses the growth modeling data. The simulated image data simulates growth on the plate relative to the disk(s). The system compares the simulated image and the pixel characteristic data to identify pixel region(s) of the second image that differ from the simulated image.

公开(公告)号：US11225682B2

公开(公告)日：2022-01-18

申请号：US15767481

申请日：2016-10-12

申请人： CureVac AG

发明人： Isabel Reichert ,  Fabian Johannes Eber ,  Christian Mayer ,  Aniela Wochner

IPC分类号： C12Q1/20 ,  C12Q1/24 ,  C12N1/20

摘要： The present invention is concerned with an automated method for obtaining at least one discrete colony from microorganisms or cells comprised in a solution. This method includes a step where acoustic liquid transfer is employed. The present invention further relates to the use of an acoustic liquid transfer device for obtaining at least one discrete colony from micro organisms or cells comprised in a solution. The present invention also relates to an automated method for determining the presence and/or quantity of microorganisms or cells potentially comprised in a solution and to the use of an acoustic liquid transfer device for determining the presence and/or quantity of microorganisms or cells potentially comprised in a solution.

公开(公告)号：US20190300927A1

公开(公告)日：2019-10-03

申请号：US16366244

申请日：2019-03-27

申请人： SELUX DIAGNOSTICS, INC.

发明人： Benjamin Spears ,  Eric Stern ,  Kelly Flentie

IPC分类号： C12Q1/20 ,  C12Q1/08

摘要： The use of metabolic probes is well-established for determining cell viability and assessing drug cytotoxicity. Resazurin-based formulations, in particular, have found utility for determining susceptibilities of microorganisms to antimicrobials, specifically through their use in antibiotic susceptibility testing (AST). There is a strong need currently to shorten AST durations, thus resazurin formulations that produce signals earlier are advantageous. This need results from the slow state of clinical microbiology testing, which may leave patients exposed to unnecessary or ineffective broad-spectrum agents for prolonged periods of time. Another slow microbiology test is Gram staining, still most often performed manually in sequential steps. Speeding Gram typing, preferably with an automated platform, would also speed time-to-results and decrease manual workloads on medical technologists in clinical microbiology laboratories.

公开(公告)号：US20150152465A1

公开(公告)日：2015-06-04

申请号：US14404849

申请日：2013-06-06

申请人： LIOFILCHEM S.R.L.

发明人： Silvio Brocco

IPC分类号： C12Q1/20

CPC分类号： C12Q1/20 ,  C12M23/10 ,  C12M23/34 ,  C12Q1/18

摘要： A device for microbiological analyses is provided. More specifically, a device is provided for standardising the crossing and allowing a perfect angle of 90° between two graduated paper strips impregnated with a predefined concentration gradient of an antimicrobial agent, for evaluating their synergistic effect on the minimum inhibitory concentration (MIC) on a bacterial culture medium.

摘要翻译： 提供微生物分析装置。 更具体地，提供了一种用于标准化交叉并且在浸渍有预定浓度梯度的抗微生物剂之间的两个分级纸条之间具有90°的完美角度的装置，以评估它们对最小抑制浓度（MIC）的协同作用 细菌培养基。

公开(公告)号：US08389233B2

公开(公告)日：2013-03-05

申请号：US12360796

申请日：2009-01-27

申请人： Mansour Samadpour

发明人： Mansour Samadpour

IPC分类号： C12Q1/08 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/10 ,  C12Q1/20 ,  G01N33/53 ,  G01N33/573 ,  C12Q1/14 ,  C12Q1/18 ,  C12Q1/22 ,  C12M1/00

CPC分类号： C12Q1/10 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/28 ,  Y10S435/849

摘要： Provided are methods for sampling, testing and validating test lots, comprising: assembling a plurality of product portions from each of a plurality of test lots and combining the portions to provide a corresponding set of test lot samples; enriching the test lot samples; removing portions of each enriched sample, and combining the removed portions to provide a modular composite sample; and testing of the modular composite sample, and individual testing of the enriched test lot samples, using at least one suitable detection assay for a target microbe or organism, wherein when such testing is negative all test lots are validated, and wherein when such testing is positive with respect to the modular composite sample, or with respect to an individual enriched test lot sample, individual test lots may nonetheless yet be validated by further testing of a portion of respective initially-negative enriched test lot samples and obtaining negative results.

摘要翻译： 提供了用于对测试批进行采样，测试和验证的方法，包括：从多个测试批中的每一个组装多个产品部分并组合该部分以提供相应的一组测试批样本; 丰富测试批样本; 去除每个富集样品的部分，并组合去除的部分以提供模块化复合样品; 使用至少一种适用于目标微生物或生物体的检测试验，对模块化复合样品进行测试，并对富集测试批样本进行单独测试，其中当这种测试为阴性时，所有测试批次都被验证，并且其中当这种测试是 对于模块化复合样品，或相对于个体富集的测试批样品，单个测试批次仍然可以通过进一步测试相应的初始负极富集的测试批样本的一部分并获得阴性结果来验证。

公开(公告)号：US20120329675A1

公开(公告)日：2012-12-27

申请号：US13384683

申请日：2010-07-20

申请人： Merle E. Olson ,  Howard Ceri

发明人： Merle E. Olson ,  Howard Ceri

IPC分类号： C40B30/06 ,  C12Q1/20

CPC分类号： C12Q1/18 ,  C12Q1/025 ,  G01N2333/21 ,  G01N2800/382

摘要： This invention is an apparatus and method for susceptibility testing one or more biofilms, for selecting one or more anti-microbial combinations with efficacy against the biofilm, and/or in treating a disease or condition mediated by the biofilm The invention includes methods for the selection of antibiotic combinations with efficacy against a specific microbial type and for the formulation of microbe-specific test plates. The invention also includes an assay system to test patient specific isolates for sensitivity to the anti-microbial combinations.

摘要翻译： 本发明是用于敏感性测试一种或多种生物膜的装置和方法，用于选择一种或多种对生物膜有效的抗微生物组合和/或治疗由生物膜介导的疾病或病症本发明包括用于选择的方法 的抗生素组合，具有针对特定微生物类型的功效和用于制备微生物特异性测试板的抗生素组合。 本发明还包括测定患者特异性分离物对抗微生物组合敏感性的测定系统。