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公开(公告)号:US20220325225A1
公开(公告)日:2022-10-13
申请号:US17621008
申请日:2020-06-26
Applicant: BD KIESTRA B.V.
Inventor: Raphael Rodolphe Marcelpoil , Martijn Kleefstra , Franciscus Feijen , Jean-Marc Volle , Mariska Okkema-Van Der Lei , Gerard Mulder , Eelke Johannes Veeninga
Abstract: An apparatus that configures a culture plate for precisely assigning coordinates to a selected colony by assigning fiducials to the culture plate using simple mechanical techniques. The fiducials correspond to the plate center and the center of a barcode label applied to the side of the culture plate by the apparatus. The apparatus then deploys mechanisms to apply coordinates to colonies identified by the user relative to the fiducials. One such mechanism is a webcam pointed at the culture plate that allows a technician to mark the colonies on a display using a computer mouse or equivalent cursor. Another mechanism deploys a laser pointer directed at the colony wherein the apparatus assigns coordinates to the location of the colony onto which the laser pointer is directed. In a third mechanism, the user views the surface of the culture plate through a viewfinder and manually registers coordinates when the view finder cross hairs are over a target colony. The selected colonies are assigned precise coordinates with reference to the two fiducials. Software is provided such that pixels in the image of the culture plate correspond to the coordinates of the culture dish relative to the fiducials.
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公开(公告)号:US20250037483A1
公开(公告)日:2025-01-30
申请号:US18664367
申请日:2024-05-15
Applicant: BD KIESTRA B.V.
Inventor: Raphael Rodolphe Marcelpoil
Abstract: An imaging method for earliest microbial growth detection. The method uses images to determined colony biomass, and the colony biomass determines when the colony can be picked for analysis for identification or antibiotic susceptibility testing. If the sample source is not a pure sample source additional incubation may be required to permit an increase in biomass of the colonies prior to pick.
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公开(公告)号:US11669971B2
公开(公告)日:2023-06-06
申请号:US17733309
申请日:2022-04-29
Applicant: BD KIESTRA B.V.
Inventor: Timothy M. Wiles , Raphael Rodolphe Marcelpoil
IPC: G06T7/00 , C12M1/00 , C12M1/34 , C12M1/36 , C12M1/22 , G06V10/40 , G06V20/69 , G06F18/214 , G06T7/11 , G06T7/13 , G06T7/90 , C12M1/12 , C12Q1/02 , G01N21/27 , G01N33/569 , G06T3/00 , G06T7/60
CPC classification number: G06T7/0016 , C12M23/10 , C12M23/22 , C12M25/06 , C12M31/02 , C12M41/14 , C12M41/46 , C12M41/48 , C12Q1/02 , G01N21/27 , G01N33/56911 , G06F18/214 , G06T3/0068 , G06T7/11 , G06T7/13 , G06T7/60 , G06T7/90 , G06V10/40 , G06V20/69 , G06V20/693 , G06V20/695 , G06V20/698 , G06T2207/10024 , G06T2207/10056 , G06T2207/10152 , G06T2207/30024
Abstract: An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.
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公开(公告)号:US11341648B2
公开(公告)日:2022-05-24
申请号:US16875082
申请日:2020-05-15
Applicant: BD KIESTRA B.V.
Inventor: Timothy M. Wiles , Raphael Rodolphe Marcelpoil
IPC: G06T7/11 , G06T7/00 , C12M1/00 , C12M1/34 , C12M1/36 , C12M1/22 , G06V10/40 , G06V20/69 , G06T7/13 , G06T7/90 , C12M1/12 , C12Q1/02 , G01N21/27 , G01N33/569 , G06K9/62 , G06T3/00 , G06T7/60
Abstract: An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.
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公开(公告)号:US10692216B2
公开(公告)日:2020-06-23
申请号:US16681333
申请日:2019-11-12
Applicant: BD KIESTRA B.V.
Inventor: Timothy M. Wiles , Raphael Rodolphe Marcelpoil
IPC: G06T7/00 , C12M1/00 , C12M1/34 , C12M1/36 , G06K9/46 , G06T7/11 , G06T7/13 , G06T7/90 , C12M1/12 , C12Q1/02 , G01N21/27 , G01N33/569 , G06K9/00 , G06K9/62 , G06T3/00 , G06T7/60 , C12M1/22
Abstract: An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.
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公开(公告)号:US11884959B2
公开(公告)日:2024-01-30
申请号:US17698518
申请日:2022-03-18
Applicant: BD KIESTRA B.V.
Inventor: Raphael Rodolphe Marcelpoil
CPC classification number: C12Q1/18 , C12Q1/20 , G01N21/17 , G06T7/0016 , G06T7/11 , G06T7/90 , G06V20/69 , G06V20/693 , G06V20/695
Abstract: An imaging system and method provides automated microbial growth detection for antibiotic sensitivity testing. A processing system having an image sensor for capturing images of an inoculated culture plate having antibiotic disks disposed on the culture media captures images of the plate at separate times (e.g., first and second images). The system generates pixel characteristic data for pixels of the second image from a comparison of the first image and second image. The pixel characteristic data may be indicative of plate growth. The system may access growth modeling data concerning the antibiotic disk(s) and generate simulated image data with a growth model function. The growth model function uses the growth modeling data. The simulated image data simulates growth on the plate relative to the disk(s). The system compares the simulated image and the pixel characteristic data to identify pixel region(s) of the second image that differ from the simulated image.
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公开(公告)号:US20210116471A1
公开(公告)日:2021-04-22
申请号:US17136670
申请日:2020-12-29
Applicant: BD KIESTRA B.V.
Inventor: Timothy R. Hansen , Rick Holtz , Martijn Kleefstra , Raphael Rodolphe Marcelpoil , Rick Pierpont , Brent Ronald Pohl , Alyssa Shedlosky , Scott Shindledecker , Edward Skevington , Kerry Lynn Smith , Timothy Wiles
Abstract: A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample. The apparatus and system optionally provide for downstream processing of samples prepared for antibiotic susceptibility testing (AST). Such apparatus includes further processing after inoculation of an AST panel for the AST test. Such further processing includes capping and transferring inoculated panels to AST instrument.
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公开(公告)号:US20200090335A1
公开(公告)日:2020-03-19
申请号:US16681333
申请日:2019-11-12
Applicant: BD KIESTRA B.V.
Inventor: Timothy M. Wiles , Raphael Rodolphe Marcelpoil
IPC: G06T7/00 , C12M1/00 , C12M1/34 , C12M1/36 , G06K9/46 , G06T7/11 , G06T7/13 , G06T7/90 , C12M1/12 , C12Q1/02 , G01N21/27 , G01N33/569 , G06K9/00 , G06K9/62 , G06T3/00 , G06T7/60 , C12M1/22
Abstract: An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.
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公开(公告)号:US11319575B2
公开(公告)日:2022-05-03
申请号:US16650087
申请日:2018-09-27
Applicant: BD KIESTRA B.V.
Inventor: Raphael Rodolphe Marcelpoil
Abstract: An imaging system and method provides automated microbial growth detection for antibiotic sensitivity testing. A processing system having an image sensor for capturing images of an inoculated culture plate having antibiotic disks disposed on the culture media captures images of the plate at separate times (e.g., first and second images). The system generates pixel characteristic data for pixels of the second image from a comparison of the first image and second image. The pixel characteristic data may be indicative of plate growth. The system may access growth modeling data concerning the antibiotic disk(s) and generate simulated image data with a growth model function. The growth model function uses the growth modeling data. The simulated image data simulates growth on the plate relative to the disk(s). The system compares the simulated image and the pixel characteristic data to identify pixel region(s) of the second image that differ from the simulated image.
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公开(公告)号:US20200291447A1
公开(公告)日:2020-09-17
申请号:US16650087
申请日:2018-09-27
Applicant: BD KIESTRA B.V.
Inventor: Raphael Rodolphe Marcelpoil
Abstract: An imaging system and method provides automated microbial growth detection for antibiotic sensitivity testing. A processing system having an image sensor for capturing images of an inoculated culture plate having antibiotic disks disposed on the culture media captures images of the plate at separate times (e.g., first and second images). The system generates pixel characteristic data for pixels of the second image from a comparison of the first image and second image. The pixel characteristic data may be indicative of plate growth. The system may access growth modeling data concerning the antibiotic disk(s) and generate simulated image data with a growth model function. The growth model function uses the growth modeling data. The simulated image data simulates growth on the plate relative to the disk(s). The system compares the simulated image and the pixel characteristic data to identify pixel region(s) of the second image that differ from the simulated image.
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