公开(公告)号：US08965196B2

公开(公告)日：2015-02-24

申请号：US14184473

申请日：2014-02-19

Applicant: Complete Genomics, Inc.

Inventor： Bryan P. Staker ,  Craig E. Uhrich

IPC: G02B21/36 ,  H04N7/18

CPC classification number: G02B21/365 ,  G01B11/272 ,  G02B21/002 ,  G02B21/025 ,  G02B21/361 ,  H04N5/37206 ,  H04N7/18

Abstract: A method and associated system for imaging high density biochemical arrays comprises one or more imaging channels that share a common objective lens and a corresponding one or more time delay integration-type imaging cameras with optical alignment mechanisms that permit independent inter-channel and intra-channel adjustment of each of four degrees: X, Y, rotation and scale. The imaging channels are configured to independently examine different spectra of the image of the biochemical arrays.

Abstract translation: 用于高密度生物化学阵列成像的方法和相关系统包括共享共同物镜的一个或多个成像通道和具有光学对准机构的相应的一个或多个时间延迟积分型成像相机，其允许独立的通道间和通道内 四度调整：X，Y，旋转和刻度。 成像通道被配置为独立地检查生物化学阵列的图像的不同光谱。

公开(公告)号：US20140378321A1

公开(公告)日：2014-12-25

申请号：US14468213

申请日：2014-08-25

Applicant: Complete Genomics, Inc.

Inventor： Radoje Drmanac

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2565/102 ,  C12Q2565/1025 ,  C12Q2565/518

Abstract: The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences. In particular, the present invention provides methods and compositions for improving the efficiency of sequencing reactions by using fewer labels to distinguish between nucleotides and by detecting nucleotides at multiple detection positions in a target sequence.

Abstract translation: 本发明涉及获取靶序列的核苷酸序列信息的方法和组合物。 特别地，本发明提供了通过使用较少的标记来区分核苷酸并通过检测目标序列中的多个检测位置的核苷酸来提高测序反应的效率的方法和组合物。

公开(公告)号：US20140232845A1

公开(公告)日：2014-08-21

申请号：US14184473

申请日：2014-02-19

Applicant: Complete Genomics, Inc.

Inventor： Bryan P. Staker ,  Craig E. Uhrich

IPC: G02B21/36

CPC classification number: G02B21/365 ,  G01B11/272 ,  G02B21/002 ,  G02B21/025 ,  G02B21/361 ,  H04N5/37206 ,  H04N7/18

Abstract: A method and associated system for imaging high density biochemical arrays comprises one or more imaging channels that share a common objective lens and a corresponding one or more time delay integration-type imaging cameras with optical alignment mechanisms that permit independent inter-channel and intra-channel adjustment of each of four degrees: X, Y, rotation and scale. The imaging channels are configured to independently examine different spectra of the image of the biochemical arrays.

Abstract translation: 用于高密度生物化学阵列成像的方法和相关系统包括共享共同物镜的一个或多个成像通道和具有光学对准机构的相应的一个或多个时间延迟积分型成像相机，其允许独立的通道间和通道内 四度调整：X，Y，旋转和刻度。 成像通道被配置为独立地检查生物化学阵列的图像的不同光谱。

公开(公告)号：US20140188396A1

公开(公告)日：2014-07-03

申请号：US14095867

申请日：2013-12-03

Applicant: Complete Genomics, Inc.

Inventor： AARON HALPERN ,  IGOR NAZARENKO

IPC: G06F19/22

CPC classification number: G16B30/00

Abstract: Mapping oligomer sequences includes receiving a set of related oligomer sequences, applying one or more key patterns derived from a set of oligomer sequence relationships to obtain one or more keys that are consistent with the set of related oligomer sequences, and locating the one or more keys in an index configured to map a plurality of possible keys to their respective candidate and/or validated locations in a reference.

Abstract translation: 映射寡聚物序列包括接收一组相关寡聚体序列，应用从一组寡聚体序列关系导出的一个或多个密钥模式以获得与所述相关寡聚体序列的集合一致的一个或多个密钥，以及定位所述一个或多个密钥 在索引中被配置为将多个可能的键映射到参考中其各自的候选和/或验证位置。

公开(公告)号：US20140152793A1

公开(公告)日：2014-06-05

申请号：US13908964

申请日：2013-06-03

Applicant: Complete Genomics, Inc

Inventor： Bryan P. Staker ,  Craig E. Uhrich

IPC: G02B21/36 ,  H04N7/18

CPC classification number: H04N5/3742 ,  G01N21/6452 ,  G01N21/6456 ,  G02B21/361 ,  G02B21/365 ,  G06T1/0007 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/10152 ,  G06T2207/30004 ,  G06T2207/30072 ,  H04N3/08 ,  H04N5/2256 ,  H04N5/3452 ,  H04N5/3532 ,  H04N5/378 ,  H04N7/18

Abstract: Imaging systems are provided for high speed, high resolution imaging of biochemical materials. In an example embodiment, an imaging system comprises an objective lens component, a line generator, a digital camera, a positioning stage, and a scan mirror. The line generator generates a line of light that is scanned across a portion of a substrate that is mounted on the positioning stage. The positioning stage moves the substrate in a particular direction that is substantially normal to an optical axis of the objective lens component. The camera collects an image of the portion of the substrate through the objective lens component. The scan mirror moves in coordination with the positioning stage, while the line of light is being scanned across the portion of the substrate and the substrate is being moved in the particular direction, in order to keep the image still with respect to the camera while the image is being collected by the camera.

Abstract translation: 提供成像系统用于生化材料的高速，高分辨率成像。 在示例性实施例中，成像系统包括物镜组件，线生成器，数字照相机，定位台和扫描镜。 线发生器产生沿安装在定位台上的衬底的一部分扫描的光线。 定位台沿着基本上垂直于物镜部件的光轴的特定方向移动基板。 相机通过物镜组件收集基板部分的图像。 扫描镜与定位台配合移动，同时沿着衬底的一部分扫描光线，并且衬底正在沿特定方向移动，以便使图像相对于照相机保持不动，而 相机正在收集图像。

公开(公告)号：US20130281305A1

公开(公告)日：2013-10-24

申请号：US13840482

申请日：2013-03-15

Applicant: COMPLETE GENOMICS, INC.

Inventor： Bill J. Peck ,  Mark Fuller ,  Daniel West ,  Anthony Delacruz

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/565 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0877 ,  G01N21/05

Abstract: Biochemical flow cells having sealed inlets and outlets are provided for performing high-volume assays on macromolecules. In one example embodiment, a flow cell with detachable inlet and outlet connectors comprises an inlet manifold, a coverslip, and a substrate disposed below the coverslip to form a reaction chamber, where the substrate is disposed to partially cover the inlet manifold such that a slit is formed along an entire edge of the substrate where fluids can flow from the inlet manifold through the slit, around substantially the entire edge of the substrate, and into the reaction chamber at equalized pressure and without bubbles. In another embodiment, a flow cell comprises an outlet manifold, two or more flow regions each connected to its own loading port via its own flow distribution funnel, each loading port connected to the outlet manifold, and plugs in a wall of the outlet manifold opposite each loading port, such that when a plug is absent from the wall of the outlet manifold, a loading tip may be inserted in its place, passing through the outlet manifold and connecting directly to a loading port.

Abstract translation: 提供具有密封入口和出口的生化流动池用于在大分子上进行高体积测定。 在一个示例性实施例中，具有可拆卸入口和出口连接器的流动池包括入口歧管，盖玻片和布置在盖玻片下方的基底，以形成反应室，其中基底设置成部分地覆盖入口歧管， 沿着衬底的整个边缘形成，其中流体可以从入口歧管流过狭缝，在基本上基本上整个边缘周围流动，并以均压和无气泡进入反应室。 在另一个实施例中，流动池包括出口歧管，两个或更多个流动区域，每个流动区域通过其自身的流量分配漏斗连接到其自己的装载端口，每个装载端口连接到出口歧管，并且插塞在出口歧管的壁上相对 每个装载端口，使得当插座从出口歧管的壁不存在时，装载尖端可插入其位置，通过出口歧管并直接连接到装载端口。

公开(公告)号：US20130222570A1

公开(公告)日：2013-08-29

申请号：US13856369

申请日：2013-04-03

Applicant: Complete Genomics, Inc.

Inventor： Bryan P. Staker ,  Craig E. Uhrich

IPC: H04N7/18

CPC classification number: G02B21/365 ,  G01B11/272 ,  G02B21/002 ,  G02B21/025 ,  G02B21/361 ,  H04N5/37206 ,  H04N7/18

Abstract: A method and associated system for imaging high density biochemical arrays comprises one or more imaging channels that share a common objective lens and a corresponding one or more time delay integration-type imaging cameras with optical alignment mechanisms that permit independent inter-channel and intra-channel adjustment of each of four degrees: X, Y, rotation and scale. The imaging channels are configured to independently examine different spectra of the image of the biochemical arrays.

Abstract translation: 用于高密度生物化学阵列成像的方法和相关系统包括共享共同物镜的一个或多个成像通道和具有光学对准机构的相应的一个或多个时间延迟积分型成像相机，其允许独立的通道间和通道内 四度调整：X，Y，旋转和刻度。 成像通道被配置为独立地检查生物化学阵列的图像的不同光谱。