公开(公告)号：US20130084602A1

公开(公告)日：2013-04-04

申请号：US13643137

申请日：2011-03-03

Applicant: Sang Jun Lee ,  Young Ok Kim ,  Bo Hye Nam ,  Hee Jeong Kong ,  Kyung Kil Kim

Inventor： Sang Jun Lee ,  Young Ok Kim ,  Bo Hye Nam ,  Hee Jeong Kong ,  Kyung Kil Kim

IPC: C12N15/70 ,  C12P21/06

CPC classification number: C12N15/625 ,  C07K2319/02 ,  C07K2319/50 ,  C12N15/70 ,  C12P21/06

Abstract: The present invention relates to expression vectors and methods for enhancing soluble expression and secretion of a heterologous protein, particularly a bulky folded active heterologous protein which has one or more transmembrane-like domains or intramolecular disulfide bonds by linking a leader peptide with acidic or basic pI and high hydrophilicity thereto; by substituting one or more amino acids within N-terminal of the heterologous protein with ones having acidic or neutral pI and high hydrophilicity; or reducing elevating GRNA value of a polynucleotide encoding the leader peptide having basic pI value and high hydrophilicity. The expression vector and the method may be used to produce of heterologous protein and to transduce of therapeutic proteins in a patient by preventing formation of insoluble inclusion body and by enhancing secretional efficiency of the heterologous protein into the periplasm or outside cell.

Abstract translation: 本发明涉及通过将前导肽与酸性或碱性pI连接来增强异源蛋白质，特别是具有一个或多个跨膜样结构域或分子内二硫键的大体积折叠活性异源蛋白质的可溶性表达和分泌的表达载体和方法 并具有高亲水性; 通过用具有酸性或中性pI和高亲水性的异源蛋白的N-末端取代一个或多个氨基酸; 或降低编码具有碱性pI值和高亲水性的前导肽的多核苷酸的升高GRNA值。 表达载体和方法可以用于产生异源蛋白质并通过防止不溶性包涵体的形成和通过增强异源蛋白质分泌到周质或外部细胞中的分泌效率来转导患者中的治疗性蛋白质。

公开(公告)号：US20130052660A1

公开(公告)日：2013-02-28

申请号：US13524868

申请日：2012-06-15

Applicant: Seung Goo Lee ,  Su-Lim Choi ,  Jong Sik Gam ,  Jae Jun Song ,  Sang Jun Lee

Inventor： Seung Goo Lee ,  Su-Lim Choi ,  Jong Sik Gam ,  Jae Jun Song ,  Sang Jun Lee

IPC: C12P21/02 ,  C12N1/21 ,  C12N5/10 ,  G01N21/64

CPC classification number: G01N21/6428 ,  G01N33/5008 ,  G01N33/542

Abstract: The present invention relates to a method for detecting protein-protein interactions in living cells, and more particularly, to a method for providing cells comprising a first construct and a second construct, wherein the first construct comprises a polynucleotide encoding a first fusion protein which comprises a bait protein, a first fluorescent protein and a CBD (cellulose-binding domain) protein, and wherein the second construct comprises a polynucleotide encoding a second fusion protein which comprises a prey protein and a second fluorescent protein so as to allow formation of inclusion bodies, and detecting interactions between the bait protein and the prey protein that are displayed by inclusion bodies, a method for isolating the prey protein bound to the bait protein using the cells comprising the constructs, the cells, and a kit for detecting protein-protein interactions, comprising the constructs.

Abstract translation: 本发明涉及一种用于检测活细胞中蛋白质 - 蛋白质相互作用的方法，更具体地说，涉及提供包含第一构建体和第二构建体的细胞的方法，其中第一构建体包含编码第一融合蛋白的多核苷酸，其包含 诱饵蛋白质，第一荧光蛋白质和CBD（纤维素结合结构域）蛋白质，并且其中第二构建体包含编码第二融合蛋白的多核苷酸，其包含猎物蛋白质和第二荧光蛋白质，以便形成包涵体 并检测由包涵体显示的诱饵蛋白和猎物蛋白之间的相互作用，使用包含构建体的细胞分离与诱饵蛋白结合的猎物蛋白的方法，所述细胞和用于检测蛋白质 - 蛋白质相互作用的试剂盒 ，包括构建体。

公开(公告)号：US20130045289A1

公开(公告)日：2013-02-21

申请号：US13511964

申请日：2010-11-25

Applicant: Si Young Cho ,  Pil Joon Park ,  Ji Hae Lee ,  Dae Bang Seo ,  Sang Jun Lee

Inventor： Si Young Cho ,  Pil Joon Park ,  Ji Hae Lee ,  Dae Bang Seo ,  Sang Jun Lee

IPC: A61K36/282 ,  A61P25/28 ,  A61P25/16 ,  A61P3/00

CPC classification number: A61K36/282 ,  A23L33/105 ,  A23V2002/00 ,  A23V2250/21 ,  A23V2200/316 ,  A23V2200/322

Abstract: Disclosed is a composition for promoting the activity of peroxisome proliferator-activated receptor-δ (PPAR-δ), which contains Artemisia vulgaris extracts or Artemisia capillaris extracts as active ingredients. The composition is effective in strengthening muscles, improving endurance and memory, and preventing and alleviating the symptoms of dementia or Parkinson's disease.

Abstract translation: 公开了一种用于促进过氧化物酶体增殖物激活受体-δ（PPAR-δ）的活性的组合物，其含有寻常蒿提取物或艾蒿提取物作为活性成分。 该组合物有效地加强肌肉，改善耐力和记忆力，以及预防和减轻痴呆或帕金森病的症状。

公开(公告)号：US20130028921A1

公开(公告)日：2013-01-31

申请号：US13637966

申请日：2011-03-31

Applicant: Hee Young Jeon ,  Hyun Jung Shin ,  Dae Bang Seo ,  Hyeon Ju Yeo ,  Sang Jun Lee

Inventor： Hee Young Jeon ,  Hyun Jung Shin ,  Dae Bang Seo ,  Hyeon Ju Yeo ,  Sang Jun Lee

IPC: A61K31/366 ,  A61P3/04 ,  A61P3/10 ,  A61P1/16 ,  A61P9/00 ,  A61P25/16 ,  A61P9/12 ,  A61P9/10 ,  A61P37/02 ,  A61P25/00 ,  A61P25/28 ,  A61K39/00 ,  A61P3/06

CPC classification number: A61K31/35 ,  A61K31/366 ,  A61K36/48

Abstract: The present invention relates to a composition which comprises as an active ingredient coumestrol or a bean extract containing coumestrol, whereby adipocyte differentiation is inhibited, the immune system of the body is improved, toxic substances are purged, and neurodegenerative disorders are prevented or improved.

Abstract translation: 本发明涉及一种组合物，其包含作为活性成分的香草雌酚或含有香豆素的豆提取物，其中脂肪细胞分化被抑制，身体的免疫系统得到改善，有毒物质被清除，并且可以预防或改善神经变性疾病。

公开(公告)号：US20130017280A1

公开(公告)日：2013-01-17

申请号：US13638813

申请日：2011-04-06

Applicant: Si Young Cho ,  Ji Hae Lee ,  Il Hong Bae ,  Min Jeong Song ,  Hyeon Ju Yeo ,  Dae Bang Seo ,  Wan Gi Kim ,  Sang Jun Lee

Inventor： Si Young Cho ,  Ji Hae Lee ,  Il Hong Bae ,  Min Jeong Song ,  Hyeon Ju Yeo ,  Dae Bang Seo ,  Wan Gi Kim ,  Sang Jun Lee

IPC: A61K36/67 ,  A61K31/202 ,  A61K36/18 ,  A61K36/752 ,  A61P21/00 ,  A61K36/282

CPC classification number: A61K36/481 ,  A61K31/202 ,  A61K36/00 ,  A61K36/185 ,  A61K36/282 ,  A61K36/355 ,  A61K36/424 ,  A61K36/44 ,  A61K36/488 ,  A61K36/537 ,  A61K36/54 ,  A61K36/67 ,  A61K36/70 ,  A61K36/73 ,  A61K36/752 ,  A61K36/815 ,  A61K36/882 ,  A61K36/9064 ,  A61K45/06 ,  A61K2300/00

Abstract: The present invention provides a composition for accelerating a change in muscle type, increasing the amount of muscle, strengthening muscle, enhancing athletic abilities, reducing lipids, suppressing the accumulation of lipids, lowering blood sugar, controlling body weight or lowering body weight, containing a PPAR-δ promoter, an AMPK promoter and a PGC1-α promoter as an active ingredient

Abstract translation: 本发明提供一种用于加速肌肉类型变化，增加肌肉量，增强肌肉，增强运动能力，减少脂质，抑制脂质积聚，降低血糖，控制体重或降低体重的组合物， PPAR-δ启动子，AMPK启动子和PGC1-α启动子作为活性成分

公开(公告)号：US08330861B2

公开(公告)日：2012-12-11

申请号：US11808067

申请日：2007-06-06

Applicant: Sang-Jun Lee

Inventor： Sang-Jun Lee

IPC: H04N5/50 ,  G06F9/44

CPC classification number: G06F8/65

Abstract: Disclosed is a display apparatus, including: an image processing part which processes an image signal; a display part which displays an image corresponding to the image signal; a storing part which stores a control program and update information related to the control program; a communication part which performs a data communication with a portable apparatus that is assessable to an upgrade server; and a controller which controls the image processing part to display the image corresponding to the image signal based on the control program, and controls the communication part to transmit the upgrade information related to the control program stored in the storing part to the upgrade server through the portable apparatus, and to receive an upgraded control program.

Abstract translation: 公开了一种显示装置，包括：处理图像信号的图像处理部; 显示与图像信号对应的图像的显示部; 存储部，其存储与所述控制程序有关的控制程序和更新信息; 通信部件，其与可升级服务器可评估的便携式设备进行数据通信; 以及控制器，其基于所述控制程序控制所述图像处理部分显示与所述图像信号对应的图像，并且控制所述通信部件通过所述控制程序将与存储在所述存储部件中的所述控制程序相关的升级信息通过 便携式设备，并且接收升级的控制程序。

公开(公告)号：US20120231601A1

公开(公告)日：2012-09-13

申请号：US13303500

申请日：2011-11-23

Applicant: Mongsup Lee ,  Inseak Hwang ,  Byoung-Yong Gwak ,  Sukhun Choi ,  Sang-Jun Lee

Inventor： Mongsup Lee ,  Inseak Hwang ,  Byoung-Yong Gwak ,  Sukhun Choi ,  Sang-Jun Lee

IPC: H01L21/02

CPC classification number: H01L28/91 ,  H01L27/10817 ,  H01L27/10852

Abstract: The present disclosure describes methods of fabricating a semiconductor device. An exemplary method includes forming a metal pattern on a substrate and etching the metal pattern using an etchant including at least an alkaline solution and an oxidant to form a metal electrode, where at least a portion of the surface of the metal electrode is uneven.

Abstract translation: 本公开描述了制造半导体器件的方法。 一种示例性方法包括在衬底上形成金属图案，并使用至少包括碱性溶液和氧化剂的蚀刻剂蚀刻金属图案以形成金属电极，其中金属电极的表面的至少一部分是不均匀的。

公开(公告)号：US20120231146A1

公开(公告)日：2012-09-13

申请号：US13508835

申请日：2010-11-09

Applicant: Bum Jin Lee ,  Yu Jin Oh ,  Jin Oh Chung ,  Sang Jun Lee

Inventor： Bum Jin Lee ,  Yu Jin Oh ,  Jin Oh Chung ,  Sang Jun Lee

IPC: A23F3/40

CPC classification number: A23F3/405

Abstract: The present invention relates to a method for preparing flavored teas by using wood, and flavored teas prepared thereby. More specifically, the present invention relates to a preparation method of flavored teas with excellent palatability and flavors in which the method uses natural wood along with the appropriate flavoring time and temperature settings, and flavored teas prepared thereby.

Abstract translation: 本发明涉及一种通过使用木材和由此制备的调味茶制备调味茶的方法。 更具体地说，本发明涉及具有优良适口性和口味的调味茶的制备方法，其中该方法使用天然木材以及合适的调味时间和温度设置，以及由此制备的调味茶。

公开(公告)号：US08249387B2

公开(公告)日：2012-08-21

申请号：US12412558

申请日：2009-03-27

Applicant: Jae Wook Jeon ,  Dong Kyun Kim ,  Seung Hun Jin ,  Ki Hoon Kim ,  Sang Jun Lee ,  Tuong Thuy Nguyen

Inventor： Jae Wook Jeon ,  Dong Kyun Kim ,  Seung Hun Jin ,  Ki Hoon Kim ,  Sang Jun Lee ,  Tuong Thuy Nguyen

IPC: G06K9/36 ,  G06K9/48 ,  G06K9/54

CPC classification number: G06K9/4633

Abstract: Disclosed herein is an image processing method and apparatus for detecting the lines of images and the start and end points of the lines. The image processing apparatus includes an edge creation unit, a Hough transform unit, and an effective parameter detection unit. The edge creation unit creates an edge image using external image data input from the outside. The Hough transform unit performs a Hough transform on information about the pixel coordinates of the edge image created by the edge creation unit. The effective parameter detection unit detects the lines of the edge image by checking effective line parameters using the results of the Hough transform. The image processing apparatus may further include an edge list for storing coordinates of effective pixels constituting the edge image and a line parameter list for storing the effective line parameters.

Abstract translation: 这里公开了一种图像处理方法和装置，用于检测图像线和线的起点和终点。 图像处理装置包括边缘创建单元，霍夫变换单元和有效参数检测单元。 边缘创建单元使用从外部输入的外部图像数据创建边缘图像。 霍夫变换单元对由边缘创建单元创建的边缘图像的像素坐标的信息执行霍夫变换。 有效参数检测单元使用霍夫变换的结果检查有效线参数来检测边缘图像的线。 图像处理装置还可以包括用于存储构成边缘图像的有效像素的坐标的边缘列表和用于存储有效线参数的线参数列表。

公开(公告)号：US08147134B2

公开(公告)日：2012-04-03

申请号：US12166496

申请日：2008-07-02

Applicant: Yong Hamb Kim ,  Kyoung Beom Lee ,  Min Kyu Lee ,  Sang Jun Lee

Inventor： Yong Hamb Kim ,  Kyoung Beom Lee ,  Min Kyu Lee ,  Sang Jun Lee

IPC: G01N25/00 ,  G01K17/00 ,  G01K11/00 ,  G01K7/00 ,  G09K11/04

CPC classification number: G01K7/00

Abstract: Disclosed herein are a method and an apparatus to identify alpha-emitting radionuclides and to measure absolute alpha radioactivity using a low temperature detector. A 4π metallic absorber which encloses a radioactive material containing alpha-emitting radionuclides absorbs the total alpha decay energy in the form of thermal energy. The corresponding temperature changes are measured by a low temperature detector attached to the 4π absorber with high energy resolution. The identification of alpha-emitting radionuclides is declared by comparing the measured temperature signal with characteristic decay energy of radionuclides. The absolute amount of the radionuclides is determined by counting the number of the pulses for each of the identified nuclides.

Abstract translation: 本文公开了一种识别α发射放射性核素并使用低温检测器测量绝对α放射性的方法和装置。 A 4＆pgr 封闭含有发射放射性核素的放射性物质的金属吸收剂以热能的形式吸收总α衰变能。 相应的温度变化由连接到4＆pgr的低温检测器测量; 吸收器具有高能量分辨率。 通过将测量的温度信号与放射性核素的特征衰减能量进行比较，可以宣布确定α发射放射性核素。 放射性核素的绝对量通过计算每个识别的核素的脉冲数来确定。