Method for preparing a sterile transgenic carp
    121.
    发明授权
    Method for preparing a sterile transgenic carp 失效
    制备无菌转基因鲤鱼的方法

    公开(公告)号:US07230156B2

    公开(公告)日:2007-06-12

    申请号:US11094286

    申请日:2005-03-31

    IPC分类号: A01K67/027 C12N15/00

    摘要: The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (β-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3′ non-coding sequence, and 3′ flanking sequence of grass carp growth hormone gene as a stop sequence. The method of the present invention is easy and convenient for operation which provides a basis for providing a technical platform of general applicability for solving the hereditary and ecological safety problems of transgenic fishes.

    摘要翻译: 本申请公开了一种制备无菌转基因鱼的方法,包括构建鲑鱼型促性腺激素释放激素的反义RNA表达载体,通过显微注射将重组DNA片段引入鲤鱼胚乳,并通过聚合酶链式反应筛选无菌转基因鱼, 放射免疫测定法,其中包含鲤鱼β肌动蛋白(β-肌动蛋白)基因启动子的表达载体,具有323bp作为靶基因的来自鲤鱼的反义鲑鱼型促性腺激素释放激素(sGnRH)基因的互补DNA片段,其包含sGnRH十肽, 促性腺激素释放激素相关肽和3'非编码序列的编码区和草鱼生长激素基因的3'侧翼序列作为停止序列。 本发明的方法操作简单方便,为提供解决转基因鱼遗传和生态安全问题的通用技术平台提供依据。

    Lost write detection and repair
    122.
    发明申请

    公开(公告)号:US20060200497A1

    公开(公告)日:2006-09-07

    申请号:US11072111

    申请日:2005-03-03

    IPC分类号: G06F17/30

    摘要: Techniques are provided for detecting lost writes so that data corruption can be avoided. According to one technique, lost writes are detected by comparing version numbers that are indicated in redo log entries with version numbers that are indicated in corresponding blocks in a standby database. According to one technique, which may be used in conjunction with the above technique, lost writes are detected by logging read operations that occur relative to a primary database, and comparing version numbers that are indicated in read log entries with version numbers that are indicated in corresponding blocks in a standby database. According to one technique, lost writes are detected by comparing, in response to read operations, (a) version numbers that are stored in a persistent in-memory cache with (b) version numbers that are indicated in corresponding blocks that are read from a primary database.

    Transportable database
    123.
    发明申请
    Transportable database 有权
    可运输数据库

    公开(公告)号:US20050256908A1

    公开(公告)日:2005-11-17

    申请号:US10966961

    申请日:2004-10-15

    IPC分类号: G06F7/00 G06F17/30

    摘要: A method and apparatus for creating a target database on a target platform based on a source database on a source platform is provided. A target database is created on the target platform based on data, metadata (for example, data in the system tablespace and control files), and external objects (for example, password files and externally stored tables) comprised within the source database. Data and metadata stored in tablespaces of the source database are converted to the format compatible with the target platform. The conversion of the data and metadata may be performed either at the source database or the target database. Redo information and undo information of the source database are not copied from the source database to the target database. Certain files, e.g., a password files, and links to externally stored object may be recreated on the target database.

    摘要翻译: 提供了一种用于在源平台上基于源数据库在目标平台上创建目标数据库的方法和装置。 基于源数据库中包含的数据,元数据(例如,系统表空间和控制文件中的数据)以及外部对象(例如,密码文件和外部存储的表),在目标平台上创建目标数据库。 存储在源数据库的表空间中的数据和元数据将转换为与目标平台兼容的格式。 可以在源数据库或目标数据库执行数据和元数据的转换。 源数据库的重做信息和撤消信息不会从源数据库复制到目标数据库。 可以在目标数据库上重新创建某些文件,例如密码文件和到外部存储对象的链接。

    Cross-correlating PMD detector
    124.
    发明授权
    Cross-correlating PMD detector 失效
    交相关PMD检测器

    公开(公告)号:US06654105B2

    公开(公告)日:2003-11-25

    申请号:US10167121

    申请日:2002-06-10

    IPC分类号: G01N2100

    CPC分类号: G02F1/0555 G02F1/0136

    摘要: A polarization mode dispersion center (306) measures PMD impairment through the cross-correlation of optical pulses. A second variable phase retarder plate (504) is oriented with polarization eigenstates at preferably 45 degree angle with respect to those eigenstates of a first variable phase retarder plate (502) to provide controllable phase retardations that can be varied and measured as an indication of PMD impairment.

    摘要翻译: 偏振模色散中心(306)通过光脉冲的互相关来测量PMD损伤。 相对于第一可变相位延迟板(502)的本征态,第二可变相位延迟板(504)以偏振本征态优选成45度角定向,以提供可改变和测量的可控相位延迟作为PMD的指示 减值。

    Survival hood
    125.
    发明授权
    Survival hood 失效
    生存罩

    公开(公告)号:US5495847A

    公开(公告)日:1996-03-05

    申请号:US442998

    申请日:1995-05-17

    申请人: Wei Hu

    发明人: Wei Hu

    CPC分类号: A62B21/00 A62B17/04

    摘要: A survival hood including a hood for the head and neck, which has an inside pocket with at least one upward open space, and a gas generator put in the pocket inside the hood to release oxygen through a chemical reaction for breathing when it is bent inwards to break an inside chemical solution container.

    摘要翻译: 一种生存罩,包括头部和颈部的罩,其具有至少一个向上开放空间的内部口袋,以及放置在罩内的口袋内的气体发生器,以便在向内弯曲时通过呼吸化学反应释放氧气 打破内部化学溶液容器。

    Toy
    126.
    外观设计
    Toy 有权

    公开(公告)号:USD993332S1

    公开(公告)日:2023-07-25

    申请号:US29815636

    申请日:2021-11-16

    申请人: Wei Hu

    设计人: Wei Hu

    摘要: FIG. 1 is a perspective view of a toy showing my new design;
    FIG. 2 is another perspective view thereof;
    FIG. 3 is a front view thereof;
    FIG. 4 is a rear view thereof;
    FIG. 5 is a left side view thereof;
    FIG. 6 is a right side view thereof;
    FIG. 7 is a top plan view thereof; and,
    FIG. 8 is a bottom plan view thereof.