公开(公告)号：US20060105472A1

公开(公告)日：2006-05-18

申请号：US10989677

申请日：2004-11-16

Applicant: Zhu Teng ,  Jeffrey Moore ,  Alan Craig ,  Gary Hickey ,  Carsten Schelp ,  Tie Wei

Inventor： Zhu Teng ,  Jeffrey Moore ,  Alan Craig ,  Gary Hickey ,  Carsten Schelp ,  Tie Wei

IPC: G01N33/543 ,  C07K14/765

CPC classification number: G01N33/548 ,  G01N33/54393

Abstract: Methods and compositions are disclosed for reducing non-specific binding in a binding assay for the determination of an analyte in a sample where one of the reagents for conducting the binding assay comprises a solid support comprising a polysaccharide. The method comprises including in an assay medium for conducting the binding assay a soluble compound comprising a protein linked to a polysaccharide. Also disclosed are methods and compositions for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The methods include as reagents a solid support comprising a polysaccharide and a soluble compound comprising a protein linked to a polysaccharide.

公开(公告)号：US6087187A

公开(公告)日：2000-07-11

申请号：US771249

申请日：1996-12-20

Applicant: Andreas Wiegand ,  Norbert Madry ,  Carsten Schelp ,  Paul Meller ,  Michael Mohlen

Inventor： Andreas Wiegand ,  Norbert Madry ,  Carsten Schelp ,  Paul Meller ,  Michael Mohlen

IPC: G01N33/531 ,  G01N5/02 ,  G01N33/543 ,  G01N33/553

CPC classification number: G01N33/54373

Abstract: The present invention relates to a piezoelectric sensor for use in diagnostic and analytic processes, in particular for the immunochemical detection of diagnostically relevant specific binding partners.

Abstract translation: 本发明涉及一种用于诊断和分析过程的压电传感器，特别是用于诊断相关特异性结合配偶体的免疫化学检测。

公开(公告)号：US08304205B2

公开(公告)日：2012-11-06

申请号：US12569714

申请日：2009-09-29

Applicant: Andreas Kappel ,  Andrea Lichte ,  Norbert Zander ,  Stefan Teigelkamp ,  Sabine Teigelkamp, legal representative ,  Carsten Schelp

Inventor： Andreas Kappel ,  Andrea Lichte ,  Norbert Zander ,  Stefan Teigelkamp ,  Carsten Schelp

IPC: C12Q1/56 ,  G01N33/53

CPC classification number: C12Q1/56 ,  G01N2333/974

Abstract: The invention relates to methods for determining the activity of a proteolytic coagulation factor of the blood coagulation cascade in a body fluid such as whole blood or plasma. A combination is provided in a reaction mixture. The combination comprises the sample and an activation agent for activating a proteolytic coagulation factor of the blood coagulation cascade or for activating the blood coagulation cascade. The effect of the activating on a reagent system comprising a cleavable moiety is evaluated. The cleavable moiety is or becomes bound to a chemiluminescent agent or a sensitizer agent or both. The chemiluminescent agent and the sensitizer agent are related in that, when in close proximity, energization of the sensitizer agent results in energization of the chemiluminescent agent. The effect of the activating is related to the activity of a proteolytic coagulation factor of the blood coagulation cascade wherein the effect is the extent of cleavage of the cleavable moiety.

Abstract translation: 本发明涉及确定体液如全血或血浆中凝血级联蛋白水解凝血因子的活性的方法。 在反应混合物中提供组合。 该组合包括样品和用于激活凝血级联的蛋白水解凝血因子或用于激活凝血级联的活化剂。 评估活化对包含可切割部分的试剂体系的影响。 可切割部分是化学发光剂或敏化剂或二者结合。 化学发光剂和敏化剂的作用在于，当紧邻时，敏化剂的通电导致化学发光剂的通电。 活化的作用与血液凝固级联的蛋白水解凝血因子的活性有关，其中效应是切割部分的切割程度。

公开(公告)号：US08153442B2

公开(公告)日：2012-04-10

申请号：US12603364

申请日：2009-10-21

Applicant: Alan R. Craig ,  Zhu Teng ,  Carsten Schelp ,  Jason Snyder ,  Christine Moran

Inventor： Alan R. Craig ,  Zhu Teng ,  Carsten Schelp ,  Jason Snyder ,  Christine Moran

IPC: G01N1/00 ,  G01N21/76 ,  G01N33/546

CPC classification number: G01N21/76 ,  G01N21/6428 ,  G01N33/54393 ,  Y10S435/968 ,  Y10T436/10 ,  Y10T436/2525

Abstract: Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with a solid support such as, for example, a particle. The support includes a chemiluminescent composition that includes a metal chelate. The present inventors observed that, when such support such as, e.g., particles, were employed in assays for the determination of an analyte, stability of signal output by the chemiluminescent composition associated with the particle was unacceptably reduced as compared to particles including other chemiluminescent compositions. In accordance with embodiments of the present invention, the stability of signal output from such particles is enhanced by including in a medium that contains the particles a sufficient amount of one or more stabilizing agents, which may be a chelating agent and/or a metal chelate such as, for example, the metal chelate that is associated with the particle.

Abstract translation: 公开了用于进行测定的方法和试剂。 本发明的方法和试剂的实施方案涉及固体支持物，例如颗粒。 载体包括包含金属螯合物的化学发光组合物。 本发明人观察到，当将这种载体例如颗粒用于测定分析物时，与包含其它化学发光组合物的颗粒相比，由与颗粒相关的化学发光组合物输出的信号的稳定性不可接受地降低 。 根据本发明的实施方案，通过在含有颗粒的介质中包含足量的一种或多种稳定剂（其可以是螯合剂和/或金属螯合物）来增加来自这些颗粒的信号输出的稳定性 例如与颗粒相关的金属螯合物。

公开(公告)号：US07867781B2

公开(公告)日：2011-01-11

申请号：US10024258

申请日：2001-12-21

Applicant: Carsten Schelp ,  Hans-Erwin Pauly

Inventor： Carsten Schelp ,  Hans-Erwin Pauly

IPC: G01N33/543

CPC classification number: G01N33/54306

Abstract: The invention relates to a method for quantitatively or qualitatively detecting an analyte in a sample, with the sample being incubated, for the purpose of avoiding, diminishing and/or detecting the high-dose hook effect, with an analyte-specific binding partner R1, which is associated with a solid phase, an analyte-specific binding partner R2, which is associated with a label L1, and an analyte-specific binding partner R3, which is associated with a label L2, and the L1-dependent measurement signal being determined either at a different time from the L2-dependent or L1 plus L2-dependent measurement signal or using a different measurement method.

Abstract translation: 本发明涉及用于定量或定性地检测样品中的分析物的方法，其中为了避免，减少和/或检测高剂量钩效应的目的，使用样品进行温育，分析物特异性结合配偶体R1， 其与固相相关联，与标记L1相关联的分析物特异性结合配偶体R2和与标记L2相关联的分析物特异性结合配偶体R3，并且确定L1依赖性测量信号 在与L2相关或L1加L2依赖测量信号的不同时间或使用不同的测量方法。

公开(公告)号：US07488608B2

公开(公告)日：2009-02-10

申请号：US11619244

申请日：2007-01-03

Applicant: Zhu Teng ,  Jeffrey L. Moore ,  Alan R. Craig ,  Gary Hickey ,  Carsten Schelp ,  Tie Quan Wei

Inventor： Zhu Teng ,  Jeffrey L. Moore ,  Alan R. Craig ,  Gary Hickey ,  Carsten Schelp ,  Tie Quan Wei

IPC: G01N33/548 ,  G01N33/544 ,  G01N33/547 ,  G01N33/53 ,  C07K17/10

CPC classification number: G01N33/548 ,  G01N33/54393

Abstract: Methods and compositions are disclosed for reducing non-specific binding in a binding assay for the determination of an analyte in a sample where one of the reagents for conducting the binding assay comprises a solid support comprising a polysaccharide. The method comprises including in an assay medium for conducting the binding assay a soluble compound comprising a protein linked to a polysaccharide. Also disclosed are methods and compositions for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The methods include as reagents a solid support comprising a polysaccharide and a soluble compound comprising a protein linked to a polysaccharide.

Abstract translation: 公开了用于在用于测定样品中分析物的结合测定中的非特异性结合的方法和组合物，其中用于进行结合测定的试剂之一包含含有多糖的固体支持物。 该方法包括在用于进行结合测定的测定培养基中包含可与多糖连接的蛋白质的可溶性化合物。 还公开了用于确定疑似含有分析物的样品中分析物的存在和/或量的方法和组合物。 所述方法包括作为试剂的固体支持物，其包含多糖和包含与多糖连接的蛋白质的可溶性化合物。

公开(公告)号：US5892144A

公开(公告)日：1999-04-06

申请号：US629262

申请日：1996-04-08

Applicant: Paul Meller ,  Norbert Madry ,  Carsten Schelp ,  Andrzej Grzegorzewski

Inventor： Paul Meller ,  Norbert Madry ,  Carsten Schelp ,  Andrzej Grzegorzewski

IPC: G01N33/483 ,  G01N5/02 ,  G01N11/00 ,  G01N11/16 ,  G01N27/00 ,  G01N33/49 ,  G01N33/543 ,  G01N29/00

CPC classification number: G01N29/036 ,  G01N33/4905 ,  G01N33/54373 ,  G01N2291/0257

Abstract: A biosensor (10) for measuring changes in viscosity, density and/or mass in a fluid to be examined, for example a blood coagulation sensor or immunoassay system. According to the invention, the reagents necessary for the test are contained in a support (13) which is put onto the measurement surface (18) of a piezoelectric element (8). As a blood coagulation sensor, the measurement surface (18) may according to the invention itself have a coagulation activator action or else be coated with coagulation activators.

Abstract translation: 一种用于测量被检流体中的粘度，密度和/或质量变化的生物传感器（10），例如血液凝固传感器或免疫测定系统。 根据本发明，测试所需的试剂包含在放置在压电元件（8）的测量表面（18）上的支撑体（13）中。 作为血液凝固传感器，根据本发明的测量表面（18）本身可以具有凝结活化剂作用或者也可以用凝结活化剂涂覆。

公开(公告)号：US09347947B2

公开(公告)日：2016-05-24

申请号：US12403299

申请日：2009-03-12

Applicant: Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

Inventor： Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

IPC: G01N21/76 ,  G01N33/58

CPC classification number: G01N33/78 ,  G01N33/582 ,  G01N2333/59

Abstract: Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.

Abstract translation: 公开了用于进行测定的方法和试剂。 本发明方法和试剂的实施方案涉及用于确定怀疑含有分析物的样品中分析物的存在和/或量的化学发光试剂。 试剂是非颗粒的并且包含用于分析物的结合配偶体和包含烯属化合物和金属螯合物的化学发光组合物。 在测定的实施方案中，提供了包含疑似含有分析物的样品，如上所述的化学发光试剂和能够产生单线态氧的敏化剂的组合。 该组合经受用于将分析物与分析物的结合配偶体结合的条件。 敏化剂被激活，并且检测由化学发光组合物产生的发光量，其中发光量与样品中分析物的量相关。

公开(公告)号：US20110091978A1

公开(公告)日：2011-04-21

申请号：US12603364

申请日：2009-10-21

Applicant: Alan R. Craig ,  Zhu Teng ,  Carsten Schelp ,  Jason Snyder ,  Christine Moran

Inventor： Alan R. Craig ,  Zhu Teng ,  Carsten Schelp ,  Jason Snyder ,  Christine Moran

IPC: G01N21/76 ,  G01N31/00

CPC classification number: G01N21/76 ,  G01N21/6428 ,  G01N33/54393 ,  Y10S435/968 ,  Y10T436/10 ,  Y10T436/2525

Abstract: Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with a solid support such as, for example, a particle. The support comprises a chemiluminescent composition that comprises a metal chelate. The present inventors observed that, when such support such as, e.g., particles, were employed in assays for the determination of an analyte, stability of signal output by the chemiluminescent composition associated with the particle was unacceptably reduced as compared to particles comprising other chemiluminescent compositions. In accordance with embodiments of the present invention, the stability of signal output from such particles is enhanced by including in a medium comprising the particles a sufficient amount of one or more stabilizing agents, which may be a chelating agent and/or a metal chelate such as, for example, the metal chelate that is associated with the particle.

Abstract translation: 公开了用于进行测定的方法和试剂。 本发明的方法和试剂的实施方案涉及固体支持物，例如颗粒。 载体包括包含金属螯合物的化学发光组合物。 本发明人观察到，当将这样的载体例如颗粒用于测定分析物时，与颗粒相关的化学发光组合物输出的信号的稳定性与包含其它化学发光组合物的颗粒的稳定性不能接受地降低 。 根据本发明的实施方案，通过在包含颗粒的介质中包含足量的一种或多种稳定剂（其可以是螯合剂和/或金属螯合物）来增加来自这些颗粒的信号输出的稳定性，例如 例如，与颗粒相关的金属螯合物。

公开(公告)号：US20100311185A1

公开(公告)日：2010-12-09

申请号：US10024258

申请日：2001-12-21

Applicant: Carsten Schelp ,  Hans-Erwin Pauly

Inventor： Carsten Schelp ,  Hans-Erwin Pauly

IPC: G01N33/53

CPC classification number: G01N33/54306

Abstract: The invention relates to a method for quantitatively or qualitatively detecting an analyte in a sample, with the sample being incubated, for the purpose of avoiding, diminishing and/or detecting the high-dose hook effect, with an analyte-specific binding partner R1, which is associated with a solid phase, an analyte-specific binding partner R2, which is associated with a label L1, and an analyte-specific binding partner R3, which is associated with a label L2, and the L1-dependent measurement signal being determined either at a different time from the L2-dependent or L1 plus L2-dependent measurement signal or using a different measurement method.

Abstract translation: 本发明涉及用于定量或定性地检测样品中的分析物的方法，其中为了避免，减少和/或检测高剂量钩效应的目的，使用样品进行温育，分析物特异性结合配偶体R1， 其与固相相关联，与标记L1相关联的分析物特异性结合配偶体R2和与标记L2相关联的分析物特异性结合配偶体R3，并且确定L1依赖性测量信号 在与L2相关或L1加L2依赖测量信号的不同时间或使用不同的测量方法。