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    Methods for transforming plants to express delta-endotoxins
    12.
    发明申请
    Methods for transforming plants to express delta-endotoxins 有权
    用于转化植物以表达δ-内毒素的方法

    公开(公告)号：US20070028324A1

    公开(公告)日：2007-02-01

    申请号：US11271658

    申请日：2005-11-10

    申请人： David Corbin ,  Charles Romano

    发明人： David Corbin ,  Charles Romano

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C12N15/8286 ,  C07K14/325 ,  C12N15/8214 ,  C12N15/8216 ,  Y02A40/162

    摘要： Disclosed is a means of controlling plant pests by a novel method of expressing Cry2A B. thuringiensis δ-endotoxins in plants. The invention comprises novel nucleic acid segments encoding proteins comprising Cry2A B. thuringiensis δ-endotoxins. The nucleic acid segments are disclosed, as are transformation vectors containing the nucleic acid segments, plants transformed with the claimed segments, methods for transforming plants, and methods of controlling plant infestation by pests.

    摘要翻译： 公开了通过在植物中表达Cry2A苏云金芽孢杆菌δ-内毒素的新方法来控制植物害虫的方法。 本发明包括编码包含Cry2A苏云金芽孢杆菌δ-内毒素的蛋白质的新型核酸片段。 公开核酸片段，以及含有核酸片段的转化载体，用要求保护的片段转化的植物，用于转化植物的方法，以及通过害虫控制植物侵染的方法。

    Expression of Cry3B insecticidal protein in plants
    13.
    发明申请
    Expression of Cry3B insecticidal protein in plants 有权

    公开(公告)号：US20050273882A1

    公开(公告)日：2005-12-08

    申请号：US11192801

    申请日：2005-07-29

    申请人： Charles Romano

    发明人： Charles Romano

    IPC分类号： A01H1/00 ,  C07K14/325 ,  C12N1/21 ,  C12N15/32 ,  C12N15/82

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： The present invention discloses methods and compositions comprising a group of novel expression cassettes which provide significantly improved levels of accumulation of Coleopteran inhibitory Cry3B and Cry3B variant amino acid sequences when these are expressed in plants. The preferred embodiments of the invention provide at least up to ten fold higher levels of insect controlling protein relative to the highest levels obtained using prior compositions. In particular, transgenic maize expressing higher levels of a protein designed to exhibit increased toxicity toward Coleopteran pests deliver superior levels of insect protection and are less likely to sponsor development of populations of target insects that are resistant to the insecticidally active protein.

    Methods for the treatment and prevention of diseases of biological conduits
    19.
    发明申请
    Methods for the treatment and prevention of diseases of biological conduits 审中-公开
    治疗和预防生物管道疾病的方法

    公开(公告)号：US20070141042A1

    公开(公告)日：2007-06-21

    申请号：US10546523

    申请日：2004-02-27

    申请人： Nicholas Franano ,  Charles Romano

    发明人： Nicholas Franano ,  Charles Romano

    IPC分类号： A61K38/48 ,  A61M31/00

    CPC分类号： A61K38/488 ,  A61K38/195 ,  A61K38/4826 ,  A61K38/4833 ,  A61K38/484 ,  A61K38/486 ,  A61K38/4886 ,  A61K2300/00

    摘要： Methods are described for dilating biological conduits by removing elastin and remodeling collagens in the wall of the conduit. Methods include the use of agents that increase the release of endogenous elastase and collagenase in the wall of the conduit, either by cells that are normally present in the wall of the conduit or by inflammatory cells that are attracted to the conduit, thereby providing additional conduit dilation. Methods also include the use of agents that increase conduit wall permeability and expose elastin and collagen fibers. Methods also include removing components of the extracellular matrix of arteries and veins leading to an inhibition of intimal hyperplasia in the wall of the vessels by decreasing biomechanical stimuli directed toward the cells in the wall of the vessel. Methods further include the use of agent that degrade micro fibers, in addition to elastin, in order to decrease the resynthesis of elastin. Methods also include the use of agent that stabilize the diameter of aneurysmal arteries by blocking cell surface receptors in the wall of the aneurysmal artery that are important in the recruitment of inflammatory cells.

    摘要翻译： 描述了通过去除导管壁中的弹性蛋白和重塑胶原来扩张生物管道的方法。 方法包括使用通过存在于管道壁中的细胞或被吸引到导管的炎性细胞来增加导管壁中内源性弹性蛋白酶和胶原酶释放的试剂，从而提供另外的导管 扩张。 方法还包括使用增加导管壁渗透性并暴露弹性蛋白和胶原纤维的试剂。 方法还包括通过减少针对血管壁中的细胞的生物力学刺激来除去动脉和静脉细胞外基质的成分，从而导致血管壁内膜增生的抑制。 方法还包括使用除弹性蛋白之外降解微纤维的试剂，以减少弹性蛋白的再合成。 方法还包括使用通过阻断在炎性细胞募集中重要的动脉瘤动脉壁中的细胞表面受体来稳定动脉瘤动脉直径的药剂。

    Nucleotide sequences encoding cry1bb proteins for enhanced expression in plants
    20.
    发明申请
    Nucleotide sequences encoding cry1bb proteins for enhanced expression in plants 审中-公开
    编码cry1bb蛋白的核苷酸序列用于增强植物中的表达

    公开(公告)号：US20060112447A1

    公开(公告)日：2006-05-25

    申请号：US10525318

    申请日：2003-08-26

    申请人： Natalia Bogdanova ,  Charles Romano

    发明人： Natalia Bogdanova ,  Charles Romano

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04 ,  A01H1/00

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： The present invention describes compositions and methods that are useful in the control of lepidopteran insect pests, and more particularly describes nucleotide sequences for use in plants that encode full-length and truncated insecticidal toxins, as well as chimeric toxins. The nucleotide sequences of the present invention exhibit modifications that, when compared to the native sequences obtained from Bacillus thuringiensis species, make them particularly useful for enhanced, improved, and or optimized expression in monocot and dicot plant species. Using methods well known to those skilled in the art the nucleotide sequences described herein can be used to transform plant cells and plant tissue in order to produce transgenic plants that express the encoded proteins, therefore conferring upon the transgenic plants the ability to resist insect infestation.

    摘要翻译： 本发明描述了可用于控制鳞翅目昆虫害虫的组合物和方法，更具体地描述了用于编码全长和截短的杀虫毒素的植物以及嵌合毒素的核苷酸序列。 本发明的核苷酸序列表现出修饰，当与从苏云金芽孢杆菌属物种获得的天然序列比较时，使它们特别可用于单子叶植物和双子叶植物物种中增强，改良和/或优化的表达。 使用本领域技术人员熟知的方法，本文所述的核苷酸序列可用于转化植物细胞和植物组织，以便产生表达编码蛋白质的转基因植物，从而赋予转基因植物抵抗昆虫侵袭的能力。