公开(公告)号：US5891314A

公开(公告)日：1999-04-06

申请号：US814812

申请日：1997-03-10

Applicant: David M. Heffelfinger ,  Craig Van Horn

Inventor： David M. Heffelfinger ,  Craig Van Horn

IPC: G01N27/447 ,  G01N27/26 ,  G01N21/00

CPC classification number: G01N27/44721

Abstract: A method and apparatus for correcting non-uniformities in the lens assembly of an electrophoresis apparatus is provided. Assuming a detector with a uniform responsivity as well as a uniform illumination source, correcting for lens non-uniformities allows accurate quantitative measurements of an electrophoresis gel to be made, thus increasing the information which can be obtained from an electrophoretic analysis. Applying the system, the non-uniformities due to the lens assembly are first characterized for a range of aperture and magnification settings. A look-up table is then created which contains the non-uniformities and/or correction data files for the lens assembly according to the aperture and magnification settings. In order to correct a sample image, the aperture and magnification settings used to obtain the sample image are provided to the system processor. These settings may be automatically obtained by the processor or manually input by the user. After the processor receives the lens settings, it applies the look-up table to determine the corresponding lens non-uniformities as well as the necessary correction file. The sample image is then normalized by dividing the sample image file by the appropriate correction file. Once normalized, the corrected sample image file may either be displayed or stored for later use.

Abstract translation: 提供了一种用于校正电泳装置的透镜组件中的不均匀性的方法和装置。 假设具有均匀响应性的检测器以及均匀的照明源，校正透镜不均匀性可以准确地定量测量电泳凝胶，从而增加可从电泳分析获得的信息。 应用该系统，由于镜头组件而引起的不均匀性首先被表征为一定范围的孔径和放大倍率设置。 然后创建一个查找表，其中包含根据孔径和放大倍率设置的镜头组件的不均匀性和/或校正数据文件。 为了校正样本图像，将用于获得样本图像的孔径和倍率设置提供给系统处理器。 这些设置可以由处理器自动获取或由用户手动输入。 在处理器接收到镜头设置后，应用查找表来确定相应的镜头不均匀性以及必要的校正文件。 然后通过将样本图像文件除以适当的校正文件来对样本图像进行归一化。 一旦标准化，校正的样本图像文件可以被显示或存储以供以后使用。

公开(公告)号：US5885531A

公开(公告)日：1999-03-23

申请号：US927556

申请日：1997-09-09

Applicant: David M. Heffelfinger ,  Franklin R. Witney

Inventor： David M. Heffelfinger ,  Franklin R. Witney

IPC: G01N21/91 ,  G01N21/25 ,  G01N21/64 ,  C12Q1/68

CPC classification number: G01N21/6458 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  Y10S435/81

Abstract: A method and apparatus of imaging fluorescence in situ hybridization (FISH) is provided. The instrument allows the user to simultaneously acquire images from several different colors. This system, used in conjunction with a combinatorial fluorescence approach, is able to create a FISH karyotype with each chromosome being painted with a different color. The optical system is continuously tunable over the detection wavelengths. In one embodiment of the system the sample is simultaneously irradiated in more than one wavelength band and the detection system uses a common path interferometer to scan through the detection wavelengths.

Abstract translation: 提供了成像荧光原位杂交（FISH）的方法和装置。 该仪器允许用户同时采集几种不同颜色的图像。 该系统与组合荧光方法结合使用，能够产生FISH染色体核型，每个染色体都涂上不同的颜色。 光学系统在检测波长上连续可调。 在系统的一个实施例中，样本同时在多于一个波长带中照射，并且检测系统使用公共路径干涉仪来扫描检测波长。

公开(公告)号：US5863504A

公开(公告)日：1999-01-26

申请号：US585303

申请日：1996-01-11

Applicant: David M. Heffelfinger ,  Franklin R. Witney

Inventor： David M. Heffelfinger ,  Franklin R. Witney

IPC: G01N21/91 ,  G01N21/25 ,  G01N21/64 ,  C12M1/40 ,  C12Q1/68

CPC classification number: G01N21/6458 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  Y10S435/81

Abstract: A method and apparatus of imaging fluorescence in situ hybridization (FISH) is provided. The instrument allows the user to simultaneously acquire images from several different colors. This system, used in conjunction with a combinatorial fluorescence approach, is able to create a FISH karyotype with each chromosome being painted with a different color. The optical system is continuously tunable over the detection wavelengths. In one embodiment of the system the sample is simultaneously irradiated in more than one wavelength band and the detection system uses a common path interferometer to scan through the detection wavelengths.

公开(公告)号：US20080116814A1

公开(公告)日：2008-05-22

申请号：US11906573

申请日：2007-10-02

Applicant: David M. Heffelfinger ,  David A. Pringle ,  David B. McDonald ,  Ashot Nalbandyan

Inventor： David M. Heffelfinger ,  David A. Pringle ,  David B. McDonald ,  Ashot Nalbandyan

IPC: G03B15/02

CPC classification number: G03B15/05 ,  G03B2215/05

Abstract: A vehicle testing lamp system comprising an onboard controller unit, an off-board controller unit, and a lamp fixture. The onboard controller unit comprises a solid state relay, the solid state relay being switchable between an idle output power setting and a high output power setting; a timing circuit; a transfer circuit; a current sensor; a current regulator; and batteries or other auxiliary power supply. The off-board controller unit comprises a power supply electrically connected to a voltage booster circuit. The lamp fixture comprises an ignitor; a shock mount; and a lamp that is electrically connected to the igniter. The off-board controller supplies power to the lamp at its idle level. The onboard transfer circuit permits the off-board power supply to be disconnected while the onboard controller unit maintains the lamp at idle, and the onboard timing circuit limits the time that the lamp remains at high output. Also disclosed is a method for using a vehicle testing lamp system.

Abstract translation: 一种车辆测试灯系统，包括车载控制器单元，车外控制器单元和灯具。 车载控制器单元包括固态继电器，固态继电器可在空转输出功率设定和高输出功率设定之间切换; 定时电路; 传输电路; 电流传感器; 现行监管机构 电池或其他辅助电源。 车外控制器单元包括电连接到升压电路的电源。 灯具包括点火器; 一个防震架; 以及电连接到点火器的灯。 车外控制器在其空闲水平下为灯供电。 车载传输电路允许车载电源断开，而车载控制器单元将灯保持在空闲状态，并且车载定时电路限制灯保持在高输出的时间。 还公开了一种使用车辆测试灯系统的方法。

公开(公告)号：US5951838A

公开(公告)日：1999-09-14

申请号：US814126

申请日：1997-03-10

Applicant: David M. Heffelfinger ,  Craig Van Horn

Inventor： David M. Heffelfinger ,  Craig Van Horn

IPC: G01N27/447 ,  G01N27/26 ,  G01N21/25 ,  G01J7/58

CPC classification number: G01N27/44721

Abstract: A method and apparatus for achieving uniform illumination in an electrophoresis apparatus is provided. Uniform illumination allows quantitative measurements of an electrophoresis gel to be made, thus increasing the information which can be obtained from an electrophoretic analysis. Uniform illumination is achieved by scanning the light source, preferably in a trans-illumination mode, across the sample gel in a direction perpendicular to the axis of the source. The light source is comprised of one or more light bulbs placed in a light tray. Variations in light intensity near the source end portions may be minimized using a variety of techniques including extended light bulbs, filters, reflectors, diffusers, or supplemental sources.

Abstract translation: 提供了一种用于在电泳装置中实现均匀照明的方法和装置。 均匀的照明可以进行电泳凝胶的定量测量，从而增加从电泳分析可以获得的信息。 通过在垂直于源的轴线的方向上扫描样品凝胶上的光源，优选以跨照明模式扫描光源来实现均匀照明。 光源由放置在光盘中的一个或多个灯泡组成。 使用包括扩展的灯泡，滤光器，反射器，漫射器或补充源在内的各种技术可以最小化源端部附近的光强度的变化。

公开(公告)号：US5799773A

公开(公告)日：1998-09-01

申请号：US814125

申请日：1997-03-10

Applicant: David M. Heffelfinger ,  Craig Van Horn

Inventor： David M. Heffelfinger ,  Craig Van Horn

IPC: G01N27/447 ,  G01N27/26

CPC classification number: G01N27/44721

Abstract: A method and apparatus for correcting an image of an electrophoresis gel for lens and detector non-uniformities is provided. The removal of such non-uniformities, in conjunction with a uniform illumination source, allows quantitative measurements of an electrophoresis gel to be made, thus increasing the information which can be obtained from an electrophoretic analysis. Lens and detector non-uniformities are removed by first determining the magnitude of the non-uniformities using a calibration standard. The calibration standard has a uniform emittance, preferably in the same wavelength band as the labeled regions of the electrophoresis gel. By placing the calibration standard in close proximity to the entrance aperture of the lens, the calibration process is relatively insensitive to illumination source non-uniformities. Thus an image of the calibration standard taken with the same lens settings as the unknown provides detailed information on the lens and detector non-uniformities. This information in conjunction with a darkfield image is used to correct the sample image.

Abstract translation: 提供了用于校正透镜和检测器不均匀性的电泳凝胶的图像的方法和装置。 结合均匀的照明源去除这种不均匀性可以进行电泳凝胶的定量测量，从而增加可从电泳分析中获得的信息。 通过首先使用校准标准确定非均匀性的大小来去除透镜和检测器不均匀性。 校准标准具有均匀的发射率，优选地与电泳凝胶的标记区域相同的波长带。 通过将校准标准放置在靠近透镜入口孔的位置，校准过程对照明源不均匀性相对不敏感。 因此，以与未知的相同的镜头设置拍摄的校准标准的图像提供关于镜头和检测器不均匀性的详细信息。 该信息与暗场图像一起用于校正样本图像。

公开(公告)号：US07948184B2

公开(公告)日：2011-05-24

申请号：US11906573

申请日：2007-10-02

Applicant: David M. Heffelfinger ,  David A. Pringle ,  David B. McDonald ,  Ashot Nalbandyan

Inventor： David M. Heffelfinger ,  David A. Pringle ,  David B. McDonald ,  Ashot Nalbandyan

IPC: B60Q1/14

CPC classification number: G03B15/05 ,  G03B2215/05

Abstract: A vehicle testing lamp system comprising an onboard controller unit, an off-board controller unit, and a lamp fixture. The onboard controller unit comprises a solid state relay, the solid state relay being switchable between an idle output power setting and a high output power setting; a timing circuit,; a transfer circuit; a current sensor; a current regulator; and batteries or other auxiliary power supply. The off-board controller unit comprises a power supply electrically connected to a voltage booster circuit. The lamp fixture comprises an ignitor; a shock mount; and a lamp that is electrically connected to the igniter. The off-board controller supplies power to the lamp at its idle level. The onboard transfer circuit permits the off-board power supply to be disconnected while the onboard controller unit maintains the lamp at idle, and the onboard timing circuit limits the time that the lamp remains at high output. Also disclosed is a method for using a vehicle testing lamp system.

Abstract translation: 一种车辆测试灯系统，包括车载控制器单元，车外控制器单元和灯具。 车载控制器单元包括固态继电器，固态继电器可在空转输出功率设定和高输出功率设定之间切换; 定时电路 传输电路; 电流传感器; 现行监管机构 电池或其他辅助电源。 车外控制器单元包括电连接到升压电路的电源。 灯具包括点火器; 一个防震架; 以及电连接到点火器的灯。 车外控制器在其空闲水平下为灯供电。 车载传输电路允许车载电源断开，而车载控制器单元将灯保持在空闲状态，并且车载定时电路限制灯保持在高输出的时间。 还公开了一种使用车辆测试灯系统的方法。

公开(公告)号：US5897760A

公开(公告)日：1999-04-27

申请号：US814127

申请日：1997-03-10

Applicant: David M. Heffelfinger ,  Craig Van Horn

Inventor： David M. Heffelfinger ,  Craig Van Horn

IPC: G01N27/447 ,  G01N27/26

CPC classification number: G01N27/44721

Abstract: A method and apparatus for correcting non-uniformities in the source and the lens assembly of an electrophoresis apparatus is provided. Assuming a detector with a uniform responsivity, correcting for source and lens non-uniformities allows quantitative measurements of an electrophoresis gel to be made, thus increasing the information which can be obtained from an electrophoretic analysis. The non-uniformities due to the illumination source are characterized by sampling a portion of the source with a linear detector array and creating a correction data file. In order to sample the source, a mirror or beamsplitter is appropriately positioned, for example along the central optical axis of the electrophoresis apparatus. Similarly, a correction data file representing the non-uniformities due to the lens assembly is created using a secondary linear source of known uniformity. After the correction data files are stored, an image of the sample is taken and a sample data file is created. The sample data file can be normalized using the correction data files thereby creating a corrected sample file which can either be displayed or stored for future use.

Abstract translation: 提供了一种用于校正电泳装置的源和透镜组件中的不均匀性的方法和装置。 假设具有均匀响应度的检测器，校正源和透镜不均匀性允许对电泳凝胶进行定量测量，从而增加可从电泳分析获得的信息。 由于照明源引起的不均匀性的特征在于用线性检测器阵列对源的一部分进行采样并创建校正数据文件。 为了对源进行采样，镜子或分束器例如沿着电泳装置的中心光轴被适当地定位。 类似地，使用已知均匀性的二次线性源产生表示由于透镜组件引起的不均匀性的校正数据文件。 在存储校正数据文件之后，取样本的图像并创建样本数据文件。 样本数据文件可以使用校正数据文件进行归一化，从而创建可以显示或存储以供将来使用的校正样本文件。

公开(公告)号：US5792610A

公开(公告)日：1998-08-11

申请号：US640658

申请日：1996-05-01

Applicant: Franklin R. Witney ,  David M. Heffelfinger

Inventor： Franklin R. Witney ,  David M. Heffelfinger

IPC: C12Q1/68 ,  G01B9/02 ,  G01B11/00 ,  G01T3/45

CPC classification number: C12Q1/6841 ,  C12Q1/6816

Abstract: The invention relates to a method of identifying the individual autosomal and sex chromosomes of a human karyotype through the use of a set of combinatorially labeled oligonucleotide probes each member thereof: (i) having a predetermined label distinguishable from the label of any other member of said set, and (ii) being capable of specifically hybridizing with one predetermined autosomal or sex chromosome of a human karyotype.

Abstract translation: 本发明涉及通过使用一组组合标记的寡核苷酸探针来鉴定人类核型的个体常染色体和性染色体的方法，每一个成员：（i）具有与所述的任何其他成员的标签可区分的预定标签 设置，和（ii）能够与人核型的一个预定的常染色体或性染色体特异性杂交。