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公开(公告)号：US08222047B2
公开(公告)日：2012-07-17
申请号：US12236486
申请日：2008-09-23
IPC分类号： G01N33/543
CPC分类号： G01N33/54313 , C12Q1/6825 , G01N33/54326 , G01N33/54386
摘要： The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles to form a plurality of complexes, releasing at least a portion of some of the plurality of complexes, determining at least a portion of the plurality of complexes released, and determining a measure of the concentration of the analyte molecules or particles in a fluid sample.
摘要翻译： 本发明涉及用于检测流体样品中的分析物分子或颗粒的系统和方法，并且在某些情况下，确定流体样品中分子或颗粒的浓度的量度。 本发明的方法可以包括固定多个分析物分子或颗粒以形成多个复合物，释放所述多个复合物中的至少一部分，确定所述多个复合物的至少一部分被释放，并且确定 测量流体样品中分析物分子或颗粒的浓度。
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公开(公告)号：US20110195852A1
公开(公告)日：2011-08-11
申请号：US12675686
申请日：2007-08-30
申请人： David R. Walt , David M. Rissin
发明人： David R. Walt , David M. Rissin
IPC分类号： C40B30/04
CPC分类号： C12Q1/40 , G01N33/54366 , G01N33/6854 , G01N2333/522 , G01N2333/938
摘要： Disclosed is a method for measuring the concentration of an analyte or analytes in a solution. Although the methods can be conducted using a number of different assay formats, in one embodiment, the assays are conducted in reaction vessels defined, at least in part, by the distal ends of fiber optic strands.
摘要翻译： 公开了一种用于测量溶液中分析物或分析物的浓度的方法。 尽管可以使用许多不同测定形式进行该方法，但是在一个实施方案中，测定在至少部分由纤维光纤束的远端限定的反应容器中进行。
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13.发明申请HIGH SENSITIVITY DETERMINATION OF THE CONCENTRATION OF ANALYTE MOLECULES OR PARTICLES IN A FLUID SAMPLE 审中-公开分析物分子或颗粒在流体样品中的浓度的高灵敏度测定公开(公告)号：US20100075862A1
公开(公告)日：2010-03-25
申请号：US12236484
申请日：2008-09-23
申请人： David C. Duffy , David M. Rissin , Linan Song , David R. Walt
发明人： David C. Duffy , David M. Rissin , Linan Song , David R. Walt
CPC分类号： G01N33/54366
摘要： The present invention relates to methods, systems, and kits for detecting, quantifying and/or analyzing a fluid sample comprising molecules or particles at low concentration. In certain embodiments, the methods for detection and/or quantifying analyte molecules in a sample comprise capturing a plurality of analyte molecules on a substrate (e.g., an array comprising a plurality of reaction vessels). The substrate may then be exposed to additional reaction components such as at least one binding ligand. The substrate may additionally be exposed to a precursor labeling agent molecule, wherein the precursor labeling agent molecule, in some cases, is converted to a labeling agent molecule, which may be detected, either directly or indirectly, which determination may be related to the presence of and/or may be employed to quantify the analyte molecules. Although the various aspects of the present invention may use a number of different assay formats, in one embodiment, the assays are conducted in a plurality of reaction vessels defined, at least in part, by the distal ends of fiber optic strands.
摘要翻译： 本发明涉及用于检测，定量和/或分析低浓度分子或颗粒的流体样品的方法，系统和试剂盒。 在某些实施方案中，用于检测和/或定量样品中的分析物分子的方法包括在基底上捕获多个分析物分子（例如，包括多个反应容器的阵列）。 然后将底物暴露于另外的反应组分，例如至少一种结合配体。 底物可以另外暴露于前体标记试剂分子，其中在某些情况下，前体标记试剂分子被转化成可直接或间接检测的标记试剂分子，该测定可能与存在 和/或可以用于定量分析物分子。 尽管本发明的各个方面可以使用多种不同的测定形式，但是在一个实施方案中，测定是在多个反应容器中进行的，所述多个反应容器至少部分由纤维束的远端限定。
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14.发明授权Methods and systems for extending dynamic range in assays for the detection of molecules or particles 有权用于扩展动态范围的方法和系统用于检测分子或颗粒公开(公告)号：US09110025B2
公开(公告)日：2015-08-18
申请号：US13037987
申请日：2011-03-01
申请人： David M. Rissin , David Fournier , David C. Duffy
发明人： David M. Rissin , David Fournier , David C. Duffy
IPC分类号： C12Q1/68 , G01N21/64 , G01N33/543
CPC分类号： G01N33/573 , G01N21/6428 , G01N21/6452 , G01N33/54313 , G01N33/54366 , G01N2201/062 , G01N2333/924
摘要： Described herein are systems and methods for extending the dynamic range of assay methods and systems used for determining the concentration of analyte molecules or particles in a fluid sample. In some embodiments, a method comprises spatially segregating a plurality of analyte molecules in a fluid sample into a plurality of locations. At least a portion of the locations may be addressed to determine the percentage of said locations containing at least one analyte molecule. Based at least in part on the percentage, a measure of the concentration of analyte molecules in the fluid sample may be determined using an analog, intensity-based detection/analysis method/system and/or a digital detection/analysis method/system. In some cases, the assay may comprise the use of a plurality of capture objects.
摘要翻译： 本文描述了用于扩展用于确定流体样品中分析物分子或颗粒的浓度的测定方法和系统的动态范围的系统和方法。 在一些实施方案中，一种方法包括将流体样品中的多个分析物分子空间分离成多个位置。 可以寻址至少一部分位置以确定包含至少一种分析物分子的所述位置的百分比。 至少部分地基于百分比，可以使用模拟，基于强度的检测/分析方法/系统和/或数字检测/分析方法/系统来确定流体样品中分析物分子的浓度的量度。 在一些情况下，测定可以包括使用多个捕获对象。
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公开(公告)号：US08846415B2
公开(公告)日：2014-09-30
申请号：US13527210
申请日：2012-06-19
IPC分类号： G01N33/543
CPC分类号： G01N33/54313 , C12Q1/6825 , G01N33/54326 , G01N33/54386
摘要： The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles to form a plurality of complexes, releasing at least a portion of some of the plurality of complexes, determining at least a portion of the plurality of complexes released, and determining a measure of the concentration of the analyte molecules or particles in a fluid sample.
摘要翻译： 本发明涉及用于检测流体样品中的分析物分子或颗粒的系统和方法，并且在某些情况下，确定流体样品中分子或颗粒的浓度的量度。 本发明的方法可以包括固定多个分析物分子或颗粒以形成多个复合物，释放所述多个复合物中的至少一部分，确定所述多个复合物的至少一部分被释放，并且确定 测量流体样品中分析物分子或颗粒的浓度。
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公开(公告)号：US20140243223A1
公开(公告)日：2014-08-28
申请号：US13527210
申请日：2012-06-19
IPC分类号： G01N33/543 , C12Q1/68
CPC分类号： G01N33/54313 , C12Q1/6825 , G01N33/54326 , G01N33/54386
摘要： The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles to form a plurality of complexes, releasing at least a portion of some of the plurality of complexes, determining at least a portion of the plurality of complexes released, and determining a measure of the concentration of the analyte molecules or particles in a fluid sample.
摘要翻译： 本发明涉及用于检测流体样品中的分析物分子或颗粒的系统和方法，并且在某些情况下，确定流体样品中分子或颗粒的浓度的量度。 本发明的方法可以包括固定多个分析物分子或颗粒以形成多个复合物，释放所述多个复合物中的至少一部分，确定所述多个复合物的至少一部分被释放，并且确定 测量流体样品中分析物分子或颗粒的浓度。
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17.发明授权Methods and arrays for target analyte detection and determination of reaction components that affect a reaction 有权用于目标分析物检测和测定影响反应的反应组分的方法和阵列公开(公告)号：US08492098B2
公开(公告)日：2013-07-23
申请号：US11707384
申请日：2007-02-16
IPC分类号： G01N33/53 , G01N33/569 , C12Q1/00 , C12Q1/68
CPC分类号： G01N33/581 , C12Q1/6837 , G01N33/543 , G01N33/54346 , G01N33/54366 , G01N33/54386 , G01N2333/938 , C12Q2527/143
摘要： Methods are described for detecting reaction components with affect a reaction. Biomolecules such as enzymes can be addressed at the single molecule level in order to discover function, detect binding partners or inhibitors, and/or measure rate constants.
摘要翻译： 描述了用于检测影响反应的反应组分的方法。 为了发现功能，检测结合配偶体或抑制剂和/或测量速率常数，可以在单分子水平处理生物分子如酶。
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18.发明申请公开(公告)号：US20120289428A1
公开(公告)日：2012-11-15
申请号：US13530979
申请日：2012-06-22
申请人： David C. Duffy , David M. Rissin , David R. Walt , David Fournier , Cheuk Kan
发明人： David C. Duffy , David M. Rissin , David R. Walt , David Fournier , Cheuk Kan
CPC分类号： G01N33/54393 , G01N33/54306 , G01N33/54313
摘要： The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.
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19.发明申请ULTRA-SENSITIVE DETECTION OF MOLECULES OR PARTICLES USING BEADS OR OTHER CAPTURE OBJECTS 有权使用珠子或其他捕获物体对分子或颗粒进行超敏感检测公开(公告)号：US20120277114A1
公开(公告)日：2012-11-01
申请号：US13531061
申请日：2012-06-22
申请人： David C. Duffy , David M. Rissin , David R. Walt , David Fournier , Cheuk Kan
发明人： David C. Duffy , David M. Rissin , David R. Walt , David Fournier , Cheuk Kan
IPC分类号： C40B30/04
CPC分类号： G01N33/54393 , G01N33/54306 , G01N33/54313
摘要： The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.
摘要翻译： 本发明涉及用于检测流体样品中的分析物分子或颗粒的系统和方法，并且在某些情况下，确定流体样品中分子或颗粒的浓度的量度。 本发明的方法可以包括相对于多个捕获物体固定多个分析物分子或颗粒。 多个捕获对象的至少一部分可以在空间上分离成多个位置。 可以至少部分地确定包含相对于捕获对象固定的分析物分子的反应容器的数量，来测量流体样品中分析物分子的浓度。 在一些情况下，该测定可另外包括包括结合配体，前体标记试剂和/或酶组分的步骤。
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20.发明申请METHODS AND SYSTEMS FOR EXTENDING DYNAMIC RANGE IN ASSAYS FOR THE DETECTION OF MOLECULES OR PARTICLES 有权用于检测分子或颗粒的测定中的动态范围的方法和系统公开(公告)号：US20110212537A1
公开(公告)日：2011-09-01
申请号：US12731136
申请日：2010-03-24
申请人： David M. Rissin , David Fournier , David C. Duffy
发明人： David M. Rissin , David Fournier , David C. Duffy
CPC分类号： G01N21/6452 , G01N33/50
摘要： Described herein are systems and methods for extending the dynamic range of assay methods and systems used for determining the concentration of analyte molecules or particles in a fluid sample. In some embodiments, a method comprises spatially segregating a plurality of analyte molecules in a fluid sample into a plurality of locations. At least a portion of the locations may be addressed to determine the percentage of said locations containing at least one analyte molecule. Based at least in part on the percentage, a measure of the concentration of analyte molecules in the fluid sample may be determined using an analog, intensity-based detection/analysis method/system and/or a digital detection/analysis method/system. In some cases, the assay may comprise the use of a plurality of capture objects.
摘要翻译： 本文描述了用于扩展用于确定流体样品中分析物分子或颗粒的浓度的测定方法和系统的动态范围的系统和方法。 在一些实施方案中，一种方法包括将流体样品中的多个分析物分子空间分离成多个位置。 可以寻址至少一部分位置以确定包含至少一种分析物分子的所述位置的百分比。 至少部分地基于百分比，可以使用模拟，基于强度的检测/分析方法/系统和/或数字检测/分析方法/系统来确定流体样品中分析物分子的浓度的量度。 在一些情况下，测定可以包括使用多个捕获对象。
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