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公开(公告)号：US20210239581A1
公开(公告)日：2021-08-05
申请号：US16781782
申请日：2020-02-04
Inventor： Rebecca K. Montange , Jeffrey W. Steaffens
IPC: G01N1/30 , G01N33/58 , G01N15/14 , G01N33/569 , C12Q1/70
Abstract: A kit and method for flow cytometry include a liquid dye concentrate for fluorescent staining of virus-size particles with a plurality of fluorogenic dyes in a liquid medium. The liquid dye concentrate includes a plurality of fluorogenic dyes and one or both of (i) the liquid medium comprising a liquid mixture including water and liquid phase organic material and (ii) disaccharide dissolved in the liquid medium.
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公开(公告)号：US20210073513A1
公开(公告)日：2021-03-11
申请号：US17099983
申请日：2020-11-17
Inventor： Daniel Porto , Timothy Jackson , Gillian Lovell , Nevine Holtz
Abstract: The disclosure provides example embodiments for automatically or semi-automatically classifying cells in microscopic images of biological samples. These embodiments include methods for selecting training sets for the development of classifier models. The disclosed selection embodiments can allow for the re-training of classifier models using training examples that have been subjected to the same or similar incubation conditions as target samples. These selection embodiments can reduce the amount of human effort required to specify the training examples. The disclosed embodiments also include the classification of individual cells based on metrics determined for the cells using phase contrast imagery and defocused brightfield imagery. These metrics can include size, shape, texture, and intensity-based metrics. These metrics are determined based on segmentation of the underlying imagery. The segmentation is based, in some embodiments, on phase contrast imagery and/or defocused brightfield imagery of biological samples.
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公开(公告)号：US20210065362A1
公开(公告)日：2021-03-04
申请号：US16644392
申请日：2018-10-30
Inventor： Daniel APPLEDORN , Eric ENDSLEY , Nevine HOLTZ , Brad NEAGLE , David ROCK , Kirk SCHROEDER
Abstract: Systems and methods are provided for automatically imaging and analyzing cell samples in an incubator. An actuated microscope operates to generate images of samples within wells of a sample container across days, weeks, or months. A plurality of images is generated for each scan of a particular well, and the images within such a scan are used to image and analysis metabolically active cells in the well. Tins analysis includes generating a “range image” by subtracting the minimum intensity value, across the scan, for each pixel from the maximum intensity value. This range image thus emphasizes cells or portions of cells that exhibit changes in activity over a scan period (e.g., neurons, myocytes, cardiomyocytes) while de-emphasizing regions that exhibit consistently high intensities when images (e.g., regions exhibiting a great deal of autofluorescence unrelated to cell activity).
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14.发明申请公开(公告)号：US20200232900A1
公开(公告)日：2020-07-23
申请号：US16253060
申请日：2019-01-21
Inventor： Tyler Gates , Jeffrey Steaffens
Abstract: Flow cytometry investigation and flow cytometry result analysis to determine an optimized dilution factor range for flow cytometry investigation of a target sample fluid stock using a flow cytometer. The optimized dilution factor range may be determined using a screening assay module of a flow cytometry system to analyze flow cytometry results determined to fall within a dynamic range of a flow cytometer to determine the optimized dilution factor range for a given flow cytometer to investigate a given target sample fluid stock. In turn, a titer assay module may be executed to prepare particle titer results based on optimized target fluid samples provided within the optimized dilution factor range. The screening assay module and/or the titer assay module may provide automated data processing with data notifications and confirmations to provide robust data analysis.
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公开(公告)号：US10520420B2
公开(公告)日：2019-12-31
申请号：US16206350
申请日：2018-11-30
Applicant: Essen Instruments, Inc.
Inventor： Andrew W. Smolak , Jean-Luc Fraikin , Erica Dawson Tenent , Kathy L. Rowlen
Abstract: Methods of evaluating particle attributes in a sample fluid subjected to flow cytometry investigation in a flow cytometer instrument, methods of processing time series signal data traces output by a flow cytometer instrument, and a flow cytometer system are provided. In the methods and systems, data points comprising time series signal data traces corresponding with detection during the flow cytometry investigation of light from the sample fluid in one or more wavelength ranges indicative of the presence of one or more particle attributes in the sample fluid are batch-processed using a batch-specific signal peak threshold determined as a function of a batch-specific noise characteristic to identify signal peaks in the batch of data points indicative of the presence of the one or more particle attributes in the sample fluid.
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Patent Agency Ranking
16.发明申请公开(公告)号：US20210327080A1
公开(公告)日：2021-10-21
申请号：US16854710
申请日：2020-04-21
Inventor： Timothy Jackson , Nevine Holtz
Abstract: Methods are provided to project depth-spanning stacks of limited depth-of-field images of a sample into a single image of the sample that can provide in-focus image information about three-dimensional contents of the image. These methods include applying filters to the stacks of images in order to identify pixels within each image that have been captured in focus. These in-focus pixels are then combined to provide the single image of the sample. Filtering of such image stacks can also allow for the determination of depth maps or other geometric information about contents of the sample. Such depth information can also be used to inform segmentation of images of the sample, e.g., by further dividing identified regions that correspond to the contents of the sample at multiple different depths.
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公开(公告)号：US11137341B2
公开(公告)日：2021-10-05
申请号：US16544842
申请日：2019-08-19
Applicant: Essen Instruments, Inc.
Inventor： Aaron Bryce Kennington
Abstract: A flow cytometer apparatus is provided herein including (a) a flow cell, (b) a fluidic pathway having a second end coupled to a first end of the flow cell, (c) a probe coupled to a first end of the fluidic pathway, (d) a sensor configured to detect one or more properties of a fluid in the fluidic pathway and positioned between the probe and the first end of the flow cell, (e) a processor in communication with the sensor, and (f) a non-transitory computer readable medium having stored therein instructions that are executable to cause the processor to perform functions including: (i) receiving, via the processor, the one or more properties of the fluid in the fluidic pathway detected by the sensor, and (ii) determining, based on the detected one or more properties of the fluid in the fluidic pathway, a presence of a separation gas in the fluid in the fluidic pathway.
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公开(公告)号：US20210181073A1
公开(公告)日：2021-06-17
申请号：US17263800
申请日：2019-08-01
Inventor： Rebecca K. Montange , Antje Schickert , Jeffrey W. Steaffens , Michael W. Olszowy
Abstract: In flow cytometry evaluation for unassociated virus-size particles stained with multiple fluorogenic dyes, the fluid sample is stained with an aqueous diluted dye formulation including multiple fluorogenic dyes. Preparation of the aqueous diluted dye formulation includes first preparatory processing to prepare a concentrated dye formulation with the fluorogenic dyes provided in a dry powder mixture mixed with and dissolved into a first liquid medium comprising DMSO. After the first preparatory processing, aqueous liquid diluent is added to dilute the first liquid medium and while the fluorogenic dyes remain in solution the liquid medium is converted to an aqueous liquid medium, which may include DMSO as a minor molar component but at a significant concentration. The stained fluid sample may include dissolved disaccharide.
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公开(公告)号：US20200371012A1
公开(公告)日：2020-11-26
申请号：US16422814
申请日：2019-05-24
Inventor： Stephen Barnes , Zachary Kane , Richard Bennett , Jason Barrett
Abstract: The disclosure provides example rinse station apparatus, cartridges, and methods for flow cytometry. The rinse station apparatus includes: (a) a cartridge docking station having a base with a recessed receptacle for a cartridge, a vertical support coupled to the base's first end and a top support coupled to the vertical support and cantilevered over the base, the top support has an opening that aligns with the cartridge's opening, (b) a locking arm coupled to the base's second end, the locking arm's free end has a ridge to cooperate with a detent coupled to the cartridge's rear wall to retain the cartridge in place, (c) a spring coupled to the vertical support's front face to apply force to the cartridge's front wall to bias the cartridge toward the locking arm, and (d) a load cell coupled to the base of the cartridge docking station, the load cell measure's the cartridge's weight.
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公开(公告)号：US20190322972A1
公开(公告)日：2019-10-24
申请号：US16458863
申请日：2019-07-01
Applicant: ESSEN INSTRUMENTS, INC. D/B/A ESSEN BIOSCIENCE, INC. , NATIONAL RESEARCH COUNCIL OF CANADA
Inventor： Kirk S. SCHROEDER , Bradley D. NEAGLE , Eric ENDSLEY , Daniel APPLEDORN , Keith MORTON
Abstract: Systems, methods, and apparatuses of controlling fluid flow are disclosed. An apparatus includes a first microplate having a first open portion and defining one or more first wells therein, a second microplate having a second open portion and defining one or more second wells therein, and a pneumatic lid constructed of styrene ethylene butylene styrene (SEBS). The pneumatic lid extends over the first open portion and the second open portion and includes one or more microfluidic channels that fluidly couple the one or more first wells to the one or more second wells. The pneumatic lid provides an airtight seal over the first microplate and the second microplate.
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