公开(公告)号：US09670480B2

公开(公告)日：2017-06-06

申请号：US12989192

申请日：2008-08-27

Applicant: Kwang Lae Hoe ,  Dong Uk Kim ,  Mi Sun Won ,  Hyang Sook Yoo ,  Dong Sup Kim ,  Han Oh Park ,  Kyung Sook Chung ,  Young Joo Jang ,  Mi Young Nam ,  Sang Jo Han ,  Shin Jung Choi ,  Seung Tae Baek ,  Hyong Bai Kim ,  Kyung Sun Heo ,  Hye Mi Lee ,  Min Ho Lee ,  Jo Young Park

Inventor： Kwang Lae Hoe ,  Dong Uk Kim ,  Mi Sun Won ,  Hyang Sook Yoo ,  Dong Sup Kim ,  Han Oh Park ,  Kyung Sook Chung ,  Young Joo Jang ,  Mi Young Nam ,  Sang Jo Han ,  Shin Jung Choi ,  Seung Tae Baek ,  Hyong Bai Kim ,  Kyung Sun Heo ,  Hye Mi Lee ,  Min Ho Lee ,  Jo Young Park

IPC: C40B50/00 ,  C40B40/02 ,  C40B30/06 ,  C12N1/00 ,  C12N15/10 ,  C40B30/00

CPC classification number: C12N15/1034 ,  C12N1/00 ,  C40B30/00 ,  C40B40/02 ,  C40B50/00

Abstract: A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.

公开(公告)号：US20140093892A1

公开(公告)日：2014-04-03

申请号：US14000221

申请日：2012-02-20

Applicant: Eun Wie Cho ,  Chang Gyu Heo ,  Jeong Heon Ko ,  Mi Kyung Woo ,  Hyang Sook Yoo ,  Hai Min Hwang

Inventor： Eun Wie Cho ,  Chang Gyu Heo ,  Jeong Heon Ko ,  Mi Kyung Woo ,  Hyang Sook Yoo ,  Hai Min Hwang

IPC: G01N33/574 ,  C07K7/06 ,  C07K16/18

CPC classification number: G01N33/574 ,  C07K7/06 ,  C07K16/18 ,  C07K16/30 ,  C07K2317/32 ,  C07K2317/34 ,  G01N33/57415 ,  G01N2333/4742

Abstract: The present invention relates to a cytokeratin 8/18 complex-specific autoantibody or a fragment comprising an antigen-binding site (paratope) thereof, the use thereof in the diagnosis of breast cancer, a polypeptide having an amino acid sequence of an epitope specifically binding to the autoantibody, a composition for diagnosing breast cancer comprising an agent capable of measuring an expression level of the autoantibody or the fragment comprising an antigen-binding site thereof, a hybridoma cell line producing the autoantibody, and a kit for diagnosing breast cancer comprising the composition of the present invention. Further, the present invention relates to a method for diagnosing breast cancer, comprising the step of detecting the cytokeratin 8/18 complex-specific autoantibody or the fragment comprising the antigen-binding site thereof using the composition of the present invention, and a method for screening a therapeutic agent for breast cancer using the autoantibody.

Abstract translation: 本发明涉及细胞角蛋白8/18复合物特异性自身抗体或包含其抗原结合位点（其相互作用）的片段，其在乳腺癌诊断中的应用，具有表位特异性结合的表位氨基酸序列的多肽 涉及自身抗体的用于诊断乳腺癌的组合物，其包含能够测量自身抗体表达水平的试剂或包含其抗原结合位点的片段，产生该自身抗体的杂交瘤细胞系和用于诊断乳腺癌的试剂盒， 本发明的组合物。 此外，本发明涉及一种诊断乳腺癌的方法，包括使用本发明的组合物检测细胞角蛋白8/18复合物特异性自身抗体或包含其抗原结合位点的片段的方法， 使用自身抗体筛选乳腺癌的治疗剂。

公开(公告)号：US08658769B2

公开(公告)日：2014-02-25

申请号：US13501426

申请日：2010-10-01

Applicant: Eun Wie Cho ,  Chang Kyu Heo ,  Mi Kyung Woo ,  Hai Min Hwang ,  Hyang Sook Yoo ,  Jeong Heun Ko

Inventor： Eun Wie Cho ,  Chang Kyu Heo ,  Mi Kyung Woo ,  Hai Min Hwang ,  Hyang Sook Yoo ,  Jeong Heun Ko

IPC: C07K16/00

CPC classification number: C07K16/40 ,  C07K2317/34 ,  G01N33/57438

Abstract: The present invention relates to an autoantibody specifically recognizing the epitope sequence of FASN (fatty acid synthase), more particularly, to the autoantibody or a fragment comprising an antigen-binding site thereof, a diagnostic composition for hepatocellular carcinoma comprising an agent capable of assessing the expression level of the autoantibody, a hybridoma cell line producing the autoantibody, a diagnostic kit for hepatocellular carcinoma comprising the composition, a method for detecting the autoantibody of hepatocellular carcinoma patient using the composition, and a method for screening a therapeutic agent for hepatocellular carcinoma by administering candidate materials for hepatocellular carcinoma treatment to confirm a reduction in the expression level of autoantibody.

Abstract translation: 本发明涉及特异性识别FASN（脂肪酸合成酶）的表位序列的自身抗体，更具体地，涉及自身抗体或其抗原结合位点的片段，包括能够评估 自身抗体的表达水平，产生自身抗体的杂交瘤细胞系，包含该组合物的肝细胞癌的诊断试剂盒，使用该组合物检测肝细胞癌患者的自身抗体的方法，以及用于筛选肝细胞癌治疗剂的方法 管理候选材料进行肝细胞癌治疗以确认自身抗体表达水平的降低。

公开(公告)号：US20120264147A1

公开(公告)日：2012-10-18

申请号：US13501426

申请日：2010-10-01

Applicant: Eun Wie Cho ,  Chang Kyu Heo ,  Mi Kyung Woo ,  Hai Min Hwang ,  Hyang Sook Yoo ,  Jeong Heun Ko

Inventor： Eun Wie Cho ,  Chang Kyu Heo ,  Mi Kyung Woo ,  Hai Min Hwang ,  Hyang Sook Yoo ,  Jeong Heun Ko

IPC: G01N33/574 ,  G01N33/566 ,  G01N33/561 ,  C07K7/06 ,  G01N33/558 ,  G01N21/64 ,  C07K16/40 ,  C12N5/20 ,  G01N33/559

CPC classification number: C07K16/40 ,  C07K2317/34 ,  G01N33/57438

Abstract: The present invention relates to an autoantibody specifically recognizing the epitope sequence of FASN (fatty acid synthase), more particularly, to the autoantibody or a fragment comprising an antigen-binding site thereof, a diagnostic composition for hepatocellular carcinoma comprising an agent capable of assessing the expression level of the autoantibody, a hybridoma cell line producing the autoantibody, a diagnostic kit for hepatocellular carcinoma comprising the composition, a method for detecting the autoantibody of hepatocellular carcinoma patient using the composition, and a method for screening a therapeutic agent for hepatocellular carcinoma by administering candidate materials for hepatocellular carcinoma treatment to confirm a reduction in the expression level of autoantibody.

Abstract translation: 本发明涉及特异性识别FASN（脂肪酸合成酶）的表位序列的自身抗体，更具体地，涉及自身抗体或其抗原结合位点的片段，包括能够评估 自身抗体的表达水平，产生自身抗体的杂交瘤细胞系，包含该组合物的肝细胞癌的诊断试剂盒，使用该组合物检测肝细胞癌患者的自身抗体的方法，以及用于筛选肝细胞癌治疗剂的方法 管理候选材料进行肝细胞癌治疗以确认自身抗体表达水平的降低。

公开(公告)号：US20100255500A1

公开(公告)日：2010-10-07

申请号：US12757004

申请日：2010-04-08

Applicant: Jeong-Heon Ko ,  Yong-Sam Kim ,  Hyang-Sook Yoo ,  Jong-Shin Yoo ,  Se-Jeong Oh ,  Cheorl-Ho Kim ,  Ok-Lye Son

Inventor： Jeong-Heon Ko ,  Yong-Sam Kim ,  Hyang-Sook Yoo ,  Jong-Shin Yoo ,  Se-Jeong Oh ,  Cheorl-Ho Kim ,  Ok-Lye Son

IPC: G01N33/53

CPC classification number: G01N33/54313 ,  G01N33/6848 ,  G01N2400/00

Abstract: The present invention relates to an analyzing method of measuring the changes of glycosylation in various glycoproteins which participate in tumorigenesis and metastasis.

Abstract translation: 本发明涉及测定参与肿瘤发生和转移的各种糖蛋白中糖基化变化的分析方法。

公开(公告)号：US20100144708A1

公开(公告)日：2010-06-10

申请号：US12376889

申请日：2007-08-10

Applicant: Gyoon Hee Han ,  Eun Hyun Choi ,  Jee Sun Yang ,  Sung Hee Hong ,  Myung Sook Kim ,  Mi Sun Won ,  Kyung Sook Chung ,  Dong Myung Kim ,  Hyang Sook Yoo ,  Hwan Mook Kim ,  Song Kyu Park ,  Ki Ho Lee ,  Chang Woo Lee ,  Youn Woong Choi ,  Yong Mi Jeong ,  Byung Gu Min

Inventor： Gyoon Hee Han ,  Eun Hyun Choi ,  Jee Sun Yang ,  Sung Hee Hong ,  Myung Sook Kim ,  Mi Sun Won ,  Kyung Sook Chung ,  Dong Myung Kim ,  Hyang Sook Yoo ,  Hwan Mook Kim ,  Song Kyu Park ,  Ki Ho Lee ,  Chang Woo Lee ,  Youn Woong Choi ,  Yong Mi Jeong ,  Byung Gu Min

IPC: A61K31/551 ,  C07D243/08 ,  C07D211/96 ,  A61K31/4965 ,  A61P35/04

CPC classification number: C07D295/185 ,  C07D243/08 ,  C07D295/26

Abstract: The present invention relates to new heterocyclic compounds containing nitrogen atoms or pharmaceutically acceptable salts thereof, a process for the preparation thereof, and a pharmaceutical composition comprising the same for treatment of cancer. The compounds according to the present invention induce DNA damage due to reactive oxygen species to activate c-abl and p53, induce RhoB to generate apoptosis, and induce cell death by down-regulating Bcl2 involved in cell survival, which is generated by dysregulated signals via the mitochondria pathway, thereby inhibiting tumor cell growth and inducing apoptosis. Accordingly, the composition according to the present invention can be used to treat cancer.

Abstract translation: 本发明涉及含有氮原子的新的杂环化合物或其药学上可接受的盐，其制备方法和包含其的用于治疗癌症的药物组合物。 根据本发明的化合物诱导由于活性氧物质引起的DNA损伤以激活c-abl和p53，诱导RhoB产生凋亡，并通过下调参与细胞存活的Bcl2诱导细胞死亡，其由失调信号通过 线粒体途径，从而抑制肿瘤细胞生长并诱导细胞凋亡。 因此，本发明的组合物可用于治疗癌症。