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    Expression of G protein-coupled receptors with altered ligand binding and/or coupling properties
    14.
    发明申请
    Expression of G protein-coupled receptors with altered ligand binding and/or coupling properties 审中-公开
    具有改变的配体结合和/或偶联性质的G蛋白偶联受体的表达

    公开(公告)号：US20070026492A1

    公开(公告)日：2007-02-01

    申请号：US11490791

    申请日：2006-07-21

    Applicant: Anupama Nadkarni ,  Joshua Trueheart

    Inventor： Anupama Nadkarni ,  Joshua Trueheart

    IPC: C12P21/06 ,  C07H21/04 ,  C12N15/74 ,  C07K14/705 ,  C07K14/395

    CPC classification number: C07K14/705 ,  A61K38/00

    Abstract: Modified forms of G protein-coupled receptors which display altered ligand binding and/or coupling properties are provided as well as cells expressing such receptors and assays utilizing these cells for screening and identifying pharmaceutically effective compounds that specifically modulate the activity of a these modified forms of G protein coupled receptors. Yeast or mammalian cells can be used to express such receptors. The subject assays enable rapid screening of large numbers of compounds (e.g., compounds in a library) to identify those which are receptor agonists or antagonists. Compositions of matter, such as these novel receptors, novel recombinant yeast cells and novel gene constructs, are also provided. The instant assays provide a convenient format for discovering compounds which can be useful in modulating cellular function, as well as in understanding the pharmacology of compounds that specifically interact with these modified forms G protein coupled receptors.

    Abstract translation: 提供显示改变的配体结合和/或偶联性质的G蛋白偶联受体的修饰形式以及表达这些受体的细胞和利用这些细胞的测定法用于筛选和鉴定特异性调节这些修饰形式的活性的药学上有效的化合物 G蛋白偶联受体。 酵母或哺乳动物细胞可用于表达此类受体。 本发明的分析使得能够快速筛选大量化合物（例如文库中的化合物），以鉴定那些是受体激动剂或拮抗剂的化合物。 还提供了诸如这些新型受体的物质组合，新型重组酵母细胞和新型基因构建体。 本发明提供了发现可用于调节细胞功能的化合物以及理解与这些修饰形式G蛋白偶联受体特异性相互作用的化合物的药理学的方便的形式。

    Functional expression of mammalian adenylyl cyclase in yeast
    18.
    发明授权
    Functional expression of mammalian adenylyl cyclase in yeast 失效
    酵母中哺乳动物腺苷酸环化酶的功能表达

    公开(公告)号：US6001553A

    公开(公告)日：1999-12-14

    申请号：US732218

    申请日：1997-01-14

    Applicant: James R. Broach ,  John P. Manfredi ,  Joshua Trueheart

    Inventor： James R. Broach ,  John P. Manfredi ,  Joshua Trueheart

    IPC: G01N33/573 ,  C12N1/19 ,  C12N9/88 ,  C12N15/09 ,  C12N15/60 ,  C12Q1/02 ,  C12Q1/00 ,  C12N1/14

    CPC classification number: C12N9/88

    Abstract: Mammalian adenylyl cyclases are functionally expressed in yeast cells. The yeast cells may be used to screen for inhibitors or activators of the adenylyl cyclase, or of a regulator of adenylyl cyclase which is functionally co-expressed in the yeast cell. Methods of identifying such inhibitors, activators and regulators are also disclosed.

    Abstract translation: PCT No.PCT / US95 / 05149 Sec。 371日期1997年1月14日 102（e）日期1997年1月14日PCT 1995年4月26日PCT PCT。 第WO95 / 30012号公报 日期：1995年11月9日玛氏腺苷酸环化酶在酵母细胞中功能表达。 酵母细胞可用于筛选腺苷酸环化酶的抑制剂或活化剂，或在酵母细胞中功能共表达的腺苷酸环化酶的调节剂。 还公开了鉴定这种抑制剂，活化剂和调节剂的方法。

    ISOLATION OF PELLET-FORMING MICROORGANISMS
    19.
    发明申请
    ISOLATION OF PELLET-FORMING MICROORGANISMS 审中-公开
    颗粒形成微生物的分离

    公开(公告)号：US20130189722A1

    公开(公告)日：2013-07-25

    申请号：US12676622

    申请日：2008-09-05

    Applicant: Joshua Trueheart ,  Jefferson Clay Lievense ,  Sara Iverson

    Inventor： Joshua Trueheart ,  Jefferson Clay Lievense ,  Sara Iverson

    IPC: G01N21/64

    CPC classification number: G01N21/6486 ,  C12N1/14 ,  C12N1/20 ,  C12N15/01 ,  C12P7/40 ,  C12Q1/04

    Abstract: The present disclosure provides technologies for identifying, characterizing and/or sorting pellet-forming microorganisms such as bacteria and/or fungi (e.g., yeast) in liquid culture, and particularly under fermentation conditions. In some embodiments, the pellet-forming microorganisms produce one or more commercial products. For example, in some embodiments the pellet-forming microorganisms produce one or more organic acids, carotenoid compounds, essential fatty acids, industrial enzymes, active pharmaceuticals, extracellular carbohydrates, and insecticidal compounds, etc. In many embodiments, the organisms are sorted while in their pellet form.

    Abstract translation: 本公开提供用于在液体培养物中，特别是在发酵条件下鉴定，表征和/或分选颗粒形成微生物如细菌和/或真菌（例如酵母）的技术。 在一些实施方案中，颗粒形成微生物产生一种或多种商业产品。 例如，在一些实施方案中，颗粒形成微生物产生一种或多种有机酸，类胡萝卜素化合物，必需脂肪酸，工业酶，活性药物，细胞外碳水化合物和杀虫化合物等。在许多实施方案中，生物体在 他们的颗粒形式。

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