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11.发明申请公开(公告)号：US20120276607A1
公开(公告)日：2012-11-01
申请号：US13540641
申请日：2012-07-03
申请人： PERRY G. CAIMI , MARK EMPTAGE , XU LI , PAUL V. VIITANEN , YAT-CHEN CHOU , MARY ANN FRANDEN , MIN ZHANG
发明人： PERRY G. CAIMI , MARK EMPTAGE , XU LI , PAUL V. VIITANEN , YAT-CHEN CHOU , MARY ANN FRANDEN , MIN ZHANG
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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12.发明授权Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate 有权在含有浓缩糖和乙酸盐的培养基中乙醇产量提高的发酵单胞菌公开(公告)号：US07897396B2
公开(公告)日：2011-03-01
申请号：US12261160
申请日：2008-10-30
申请人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
发明人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.
摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 当与含有木糖的混合糖介质，特别是在乙酸盐存在下培养时，发现与亲本菌株相比，进一步工程化以减少himA基因活性的木糖酶发酵单胞菌菌株具有增加的乙醇产量。
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公开(公告)号：US07846712B2
公开(公告)日：2010-12-07
申请号：US11912493
申请日：2007-03-19
申请人： Min Zhang , Arjun Singh , Eric Knoshaug , Mary Ann Franden , Eric Jarvis , Pirkko Suominen
发明人： Min Zhang , Arjun Singh , Eric Knoshaug , Mary Ann Franden , Eric Jarvis , Pirkko Suominen
IPC分类号： C12N1/14
摘要： An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. Methods of producing ethanol include utilizing these modified yeast strains.
摘要翻译： 提供利用酵母菌株的L-阿拉伯糖，用于通过引入和表达细菌araA，araB和araD基因来生产乙醇。 还将L-阿拉伯糖转运蛋白引入酵母中以增强阿拉伯糖的摄取。 酵母携带额外的基因组突变，使其能够消耗L-阿拉伯糖，甚至作为唯一的碳源，并产生乙醇。 生产乙醇的方法包括利用这些改良的酵母菌株。
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公开(公告)号：US20100144041A1
公开(公告)日：2010-06-10
申请号：US11912493
申请日：2007-03-19
申请人： Min Zhang , Arjun Singh , Pirkko Suominen , Eric Knoshaug , Mary Ann Franden , Eric Jarvis
发明人： Min Zhang , Arjun Singh , Pirkko Suominen , Eric Knoshaug , Mary Ann Franden , Eric Jarvis
摘要： An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. Methods of producing ethanol include utilizing these modified yeast strains.
摘要翻译： 提供利用酵母菌株的L-阿拉伯糖，用于通过引入和表达细菌araA，araB和araD基因来生产乙醇。 还将L-阿拉伯糖转运蛋白引入酵母中以增强阿拉伯糖的摄取。 酵母携带额外的基因组突变，使其能够消耗L-阿拉伯糖，甚至作为唯一的碳源，并产生乙醇。 生产乙醇的方法包括利用这些改良的酵母菌株。
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公开(公告)号：US07629156B2
公开(公告)日：2009-12-08
申请号：US11862522
申请日：2007-09-27
申请人： Paul V. Viitanen , Carol M. Mc Cutchen , Xu Li , Mark Emptage , Perry G. Caimi , Min Zhang , Yat-Chen Chou , Mary Ann Franden
发明人： Paul V. Viitanen , Carol M. Mc Cutchen , Xu Li , Mark Emptage , Perry G. Caimi , Min Zhang , Yat-Chen Chou , Mary Ann Franden
摘要： Xylose-utilizing Z. mobilis strains were found to have improved ethanol production when grown in medium containing mixed sugars including xylose if sorbitol or mannitol was included in the medium. The effect was seen in concentrations of mixed sugars where no growth lag period occurs, as well as in higher sugars concentrations.
摘要翻译： 当发现含木糖的运动发酵单胞菌菌株在含有木糖的混合糖的培养基中生长时，如果在培养基中包含山梨糖醇或甘露醇，则发现其具有改善的乙醇生产。 在没有生长滞后期的混合糖的浓度以及较高的糖浓度下观察到效果。
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16.发明申请ZYMOMONAS WITH IMPROVED ETHANOL PRODUCTION IN MEDIUM CONTAINING CONCENTRATED SUGARS AND ACETATE 有权含有浓缩糖和乙酸的中等乙醇生产的ZYMOMONASAS公开(公告)号：US20090221078A1
公开(公告)日：2009-09-03
申请号：US12261166
申请日：2008-10-30
申请人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
发明人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
CPC分类号： C12N15/52 , C12N9/1022 , C12N9/1205 , C12N9/92 , C12N15/74 , C12P7/065 , Y02E50/16 , Y02E50/17
摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.
摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 与在亲本菌株相比，当在含有木糖和乙酸酯的培养基中培养时，发现进一步工程化以减少himA基因活性的木糖酶发酵菌具有增加的乙醇产量。
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17.发明申请ZYMOMONAS WITH IMPROVED ETHANOL PRODUCTION IN MEDIUM CONTAINING CONCENTRATED SUGARS AND ACETATE 有权含有浓缩糖和乙酸的中等乙醇生产的ZYMOMONASAS公开(公告)号：US20090203099A1
公开(公告)日：2009-08-13
申请号：US12261160
申请日：2008-10-30
申请人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
发明人： Perry G. Caimi , Yat-Chen Chou , Mary Ann Franden , Kyle Knoke , Luan Tao , Paul V. Viitanen , Min Zhang , Yuying Zhang
IPC分类号： C12P7/06
摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.
摘要翻译： 通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。 当与含有木糖的混合糖介质，特别是在乙酸盐存在下培养时，发现与亲本菌株相比，进一步工程化以减少himA基因活性的木糖酶发酵单胞菌菌株具有增加的乙醇产量。
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18.发明授权Recombinant lactobacillus for fermentation of xylose to lactic acid and lactate 失效用于将木糖发酵成乳酸和乳酸的重组乳杆菌
公开(公告)号：US5798237A
公开(公告)日：1998-08-25
申请号：US541632
申请日：1995-10-10
CPC分类号： C12N9/1205 , C12N15/746 , C12N9/90 , C12P7/56
摘要： A recombinant Lactobacillus MONT4 is provided which has been genetically engineered with xylose isomerase and xylulokinase genes from Lactobacillus pentosus to impart to the Lactobacillus MONT4 the ability to ferment lignocellulosic biomass containing xylose to lactic acid.
摘要翻译： 提供了重组的乳杆菌MONT4，其已经用来自戊糖乳杆菌的木糖异构酶和木酮糖激酶基因进行了遗传工程改造，以赋予乳酸菌MONT4发酵木质纤维素生物质含木糖至乳酸的能力。
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