Single zymomonas mobilis strain for xylose and arabinose fermentation
    4.
    发明授权
    Single zymomonas mobilis strain for xylose and arabinose fermentation 失效
    用于木糖和阿拉伯糖发酵的单一运动发酵单胞菌菌株

    公开(公告)号:US5843760A

    公开(公告)日:1998-12-01

    申请号:US851767

    申请日:1997-05-06

    摘要: This invention relates to single microorganisms which normally do not ferment pentose sugars which are genetically altered to ferment the pentose sugars, xylose and arabinose, to produce ethanol, and a fermentation process utilizing the same. Examples include Zymomonas mobilis which has been transformed with a combination of E. coli genes for xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose 5-phosphate 4-epimerase, transaldolase and transketolase. Expression of added genes are under the control of Z. mobilis promoters. These newly created microorganisms are useful for fermenting glucose, xylose and arabinose, produced by hydrolysis of hemicellulose and cellulose or starch, to produce ethanol.

    摘要翻译: 本发明涉及通常不发酵戊糖的单一微生物,其经遗传改变以发酵戊糖,木糖和阿拉伯糖以产生乙醇,以及利用该微生物的发酵方法。 实例包括用木糖异构酶的大肠杆菌基因,木酮糖激酶,L-阿拉伯糖异构酶,L-核糖激酶,L-核酮糖-5-磷酸4-差向异构酶,转醛醇酶和转酮酶的组合转化的运动发酵单胞菌。 添加基因的表达在运动发酵单胞菌启动子的控制下。 这些新产生的微生物可用于发酵通过半纤维素和纤维素或淀粉的水解产生的葡萄糖,木糖和阿拉伯糖,以产生乙醇。

    L-arabinose fermenting yeast
    8.
    发明授权
    L-arabinose fermenting yeast 有权
    L-阿拉伯糖发酵酵母

    公开(公告)号:US08372626B2

    公开(公告)日:2013-02-12

    申请号:US12909523

    申请日:2010-10-21

    IPC分类号: C12N1/14

    CPC分类号: C12N15/81 C12P7/06 C12P7/08

    摘要: An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. A yeast strain engineered to metabolize arabinose through a novel pathway is also disclosed. Methods of producing ethanol include utilizing these modified yeast strains.

    摘要翻译: 提供利用酵母菌株的L-阿拉伯糖,用于通过引入和表达细菌araA,araB和araD基因来生产乙醇。 还将L-阿拉伯糖转运蛋白引入酵母中以增强阿拉伯糖的摄取。 酵母携带额外的基因组突变,使其能够消耗L-阿拉伯糖,甚至作为唯一的碳源,并产生乙醇。 还公开了通过新途径工程化代谢阿拉伯糖的酵母菌株。 生产乙醇的方法包括利用这些改良的酵母菌株。