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公开(公告)号：US07067300B2
公开(公告)日：2006-06-27
申请号：US10277249
申请日：2002-10-21
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
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公开(公告)号：US08465961B2
公开(公告)日：2013-06-18
申请号：US13540641
申请日：2012-07-03
申请人： Perry G Caimi , Mark Emptage , Xu Li , Paul V Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： Perry G Caimi , Mark Emptage , Xu Li , Paul V Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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公开(公告)号：US20130177947A1
公开(公告)日：2013-07-11
申请号：US13498069
申请日：2010-09-22
申请人： Benjamin Bower , Megan Yee Hsi , Thijs Kaper , Bradley R. Keleman , Suzanne E. Lantz , Edmund A. Larenas , Colin Mitchinson , Steven Kim , William D. Hitz , Mark Emptage , Keith Dumont Wing
发明人： Benjamin Bower , Megan Yee Hsi , Thijs Kaper , Bradley R. Keleman , Suzanne E. Lantz , Edmund A. Larenas , Colin Mitchinson , Steven Kim , William D. Hitz , Mark Emptage , Keith Dumont Wing
CPC分类号： C12N9/248 , C12N9/0006 , C12N9/0065 , C12N9/16 , C12N9/20 , C12N9/2402 , C12N9/2437 , C12N9/2445 , C12N9/2485 , C12P19/00 , C12P19/02 , C12P19/14 , C12Y302/01 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01037 , C12Y302/01055 , Y02E50/343
摘要： The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars.
摘要翻译： 本公开一般涉及糖基水解酶，包含这些酶的组合物，以及使用酶和组合物的方法，例如将纤维素和半纤维素材料糖化为糖。
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公开(公告)号：US08247208B2
公开(公告)日：2012-08-21
申请号：US12641642
申请日：2009-12-18
申请人： Perry G. Caimi , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： Perry G. Caimi , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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公开(公告)号：US20060148053A1
公开(公告)日：2006-07-06
申请号：US11282497
申请日：2006-01-16
申请人： Mark Emptage , Sharon Haynie , Lisa Laffend , Jeff Pucci , Gregory Whited
发明人： Mark Emptage , Sharon Haynie , Lisa Laffend , Jeff Pucci , Gregory Whited
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
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6.发明申请公开(公告)号：US20110014670A1
公开(公告)日：2011-01-20
申请号：US12641642
申请日：2009-12-18
申请人： PERRY G. CAIMI , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
发明人： PERRY G. CAIMI , Mark Emptage , Xu Li , Paul V. Viitanen , Yat-Chen Chou , Mary Ann Franden , Min Zhang
摘要： Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
摘要翻译： 使用适应方法获得使用具有改善的木糖利用的发酵单菌的木糖菌株和在胁迫条件下发酵期间的乙醇生产。 适应性涉及使用含有高糖，乙酸，氨和乙醇的培养基中的酵母单胞菌连续生长木糖。
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7.发明申请PROCESS FOR THE BIOLOGICAL PRODUCTION OF 1,3-PROPANEDIOL WITH HIGH TITER 审中-公开具有高TITER的1,3-丙二醇的生物生产工艺公开(公告)号：US20090253192A1
公开(公告)日：2009-10-08
申请号：US12371745
申请日：2009-02-16
申请人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend
发明人： Mark Emptage , Sharon L. Haynie , Lisa A. Laffend
IPC分类号： C12N1/21
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
摘要翻译： 本发明提供了从单一微生物中的可发酵碳源生物制备1,3-丙二醇的改进方法。 在本发明的一个方面，通过使用用肺炎克雷伯杆菌dha调节基因dhaR或orfY，dhaT，orfX或ff转化的大肠杆菌来实现葡萄糖转化为1,3-丙二醇的改进方法， dhaB1，dhaB2，dhaB3和orfZ，所有这些基因排列在与野生型肺炎克雷伯菌中发现的相同的遗传组织中。 在本发明的另一方面，使用含有编码G3PDH，G3P磷酸酶，脱水酶和脱水酶活化因子的重组大肠杆菌从与葡萄糖生产1,3-丙二醇的改进方法相比， 使用含有编码G3PDH，G3P磷酸酶，脱水酶，脱水酶活化因子和1,3-丙二醇氧化还原酶（dhaT）的基因的重组大肠杆菌的方法。 显着改善的方法依赖于在大肠杆菌中存在编码足以将3-羟基丙醛转化成1,3-丙二醇的非特异性催化活性的基因。
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8.发明授权公开(公告)号：US07504250B2
公开(公告)日：2009-03-17
申请号：US11282497
申请日：2006-01-16
IPC分类号： C12N1/20 , C12N9/00 , C12N9/02 , C12N15/00 , C12N9/04 , C12Q1/68 , C07H21/04 , C12Q1/00 , C12Q21/04
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
摘要翻译： 本发明提供了从单一微生物中的可发酵碳源生物制备1,3-丙二醇的改进方法。 在本发明的一个方面，通过使用用肺炎克雷伯杆菌dha调节基因dhaR或orfY，dhaT，orfX或ff转化的大肠杆菌来实现葡萄糖转化为1,3-丙二醇的改进方法， dhaB1，dhaB2，dhaB3和orfZ，所有这些基因排列在与野生型肺炎克雷伯菌中发现的相同的遗传组织中。 在本发明的另一方面，使用含有编码G3PDH，G3P磷酸酶，脱水酶和脱水酶活化因子的重组大肠杆菌从与葡萄糖生产1,3-丙二醇的改进方法相比， 使用含有编码G3PDH，G3P磷酸酶，脱水酶，脱水酶活化因子和1,3-丙二醇氧化还原酶（dhaT）的基因的重组大肠杆菌的方法。 显着改善的方法依赖于在大肠杆菌中存在编码足以将3-羟基丙醛转化成1,3-丙二醇的非特异性催化活性的基因。
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9.发明申请公开(公告)号：US20080081358A1
公开(公告)日：2008-04-03
申请号：US11862522
申请日：2007-09-27
申请人： PAUL VIITANEN , Carol Mc Cutchen , Xu Li , Mark Emptage , Perry Caimi , Min Zhang , Yat-Chen Chou , Mary Franden
发明人： PAUL VIITANEN , Carol Mc Cutchen , Xu Li , Mark Emptage , Perry Caimi , Min Zhang , Yat-Chen Chou , Mary Franden
摘要： Xylose-utilizing Z. mobilis strains were found to have improved ethanol production when grown in medium containing mixed sugars including xylose if sorbitol or mannitol was included in the medium. The effect was seen in concentrations of mixed sugars where no growth lag period occurs, as well as in higher sugars concentrations.
摘要翻译： 当发现含木糖的运动发酵单胞菌菌株在含有木糖的混合糖的培养基中生长时，如果在培养基中包含山梨糖醇或甘露醇，则发现其具有改善的乙醇生产。 在没有生长滞后期的混合糖的浓度以及较高的糖浓度下观察到效果。
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公开(公告)号：US06514733B1
公开(公告)日：2003-02-04
申请号：US09641652
申请日：2000-08-18
IPC分类号： C12P702
CPC分类号： C12N9/0006 , C12N9/1205 , C12N9/90 , C12N15/52 , C12P7/18
摘要： The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
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