利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

20 条记录 (耗时 0.023 秒)

    RATIONALLY DESIGNED MEDIA FOR CELL CULTURE
    11.
    发明申请
    RATIONALLY DESIGNED MEDIA FOR CELL CULTURE 审中-公开
    用于细胞培养的专门设计的媒体

    公开(公告)号：US20130084593A1

    公开(公告)日：2013-04-04

    申请号：US13561395

    申请日：2012-07-30

    申请人： Yen-Tung Luan ,  Wenge Wang ,  Ryan Nolan ,  Denis Drapeau

    发明人： Yen-Tung Luan ,  Wenge Wang ,  Ryan Nolan ,  Denis Drapeau

    IPC分类号： C12Q1/02

    CPC分类号： C12Q1/02 ,  C12N5/0018 ,  C12N2500/32

    摘要： This invention relates to methods for rationally designing cell culture media for use in cell cultures, e.g., cell cultures employed in polypeptide production; cell culture media designed with the disclosed methods; methods of producing a polypeptide of interest, e.g., an antibody, using such media; polypeptides produced using the methods and media disclosed herein; and pharmaceuticals compositions containing such polypeptides. The rationally designed media contain a concentration of an amino acid that is calculated for use in cell mass, a concentration of the amino acid that is calculated for use in cell maintenance, and a concentration of the amino acid that is calculated for incorporation into the polypeptide of interest.

    摘要翻译： 本发明涉及用于合理设计用于细胞培养物的细胞培养基的方法，例如用于多肽生产的细胞培养物; 用公开的方法设计的细胞培养基; 使用这种培养基生产感兴趣多肽的方法，例如抗体; 使用本文公开的方法和介质产生的多肽; 和含有这种多肽的药物组合物。 合理设计的培养基含有计算用于细胞质量的氨基酸浓度，计算用于细胞维持的氨基酸的浓度以及计算用于掺入多肽的氨基酸的浓度 出于兴趣。

    Production of polypeptides
    12.
    发明授权
    Production of polypeptides 有权
    生产多肽

    公开(公告)号：US07294484B2

    公开(公告)日：2007-11-13

    申请号：US11213308

    申请日：2005-08-25

    申请人： Denis Drapeau ,  Yen-Tung Luan ,  James R. Mercer ,  Wenge Wang ,  Daniel R. Lasko

    发明人： Denis Drapeau ,  Yen-Tung Luan ,  James R. Mercer ,  Wenge Wang ,  Daniel R. Lasko

    IPC分类号： C12P21/06

    CPC分类号： C12P21/02 ,  C07K16/18 ,  C07K16/22 ,  C07K16/2896 ,  C07K2317/24 ,  C12N5/0037 ,  C12N2500/22 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/60 ,  C12N2510/02

    摘要： An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.

    摘要翻译： 特别是在以下一个或多个特征的介质中大规模生产蛋白质和/或多肽的改进系统：i）累积的氨基酸浓度大于约70mM; ii）累积谷氨酰胺的累积天冬酰胺摩尔比小于约2; iii）累积谷氨酰胺与累积总氨基酸的摩尔比小于约0.2; iv）摩尔累计无机离子与累积总氨基酸比例在约0.4至1之间; 或v）组合的累积谷氨酰胺和约16至36mM之间的累积天冬酰胺浓度。 使用这样的系统允许高水平的蛋白质产生并减少某些不需要的因素例如铵和/或乳酸盐的积累。 此外，提供包括温度变化的培养方法，通常包括当培养物达到其最大细胞密度的约20-80％时的温度降低。 或者或另外，本发明提供了在达到峰值之后，培养物中的乳酸盐和/或铵水平随时间降低的方法。

    Production of TNFR-Ig
    13.
    发明授权
    Production of TNFR-Ig 有权
    生产TNFR-Ig

    公开(公告)号：US07300773B2

    公开(公告)日：2007-11-27

    申请号：US11213633

    申请日：2005-08-25

    申请人： Denis Drapeau ,  Yen-Tung Luan ,  James R. Mercer ,  Wenge Wang ,  Daniel R. Lasko

    发明人： Denis Drapeau ,  Yen-Tung Luan ,  James R. Mercer ,  Wenge Wang ,  Daniel R. Lasko

    IPC分类号： C12P21/06

    CPC分类号： C12P21/02 ,  C07K14/7151 ,  C07K2319/30 ,  C12N2500/32 ,  C12N2500/90 ,  C12N2500/95 ,  C12P21/00

    摘要： An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.

    摘要翻译： 特别是在以下一个或多个特征的介质中大规模生产蛋白质和/或多肽的改进系统：i）累积的氨基酸浓度大于约70mM; ii）累积谷氨酰胺的累积天冬酰胺摩尔比小于约2; iii）累积谷氨酰胺与累积总氨基酸的摩尔比小于约0.2; iv）摩尔累计无机离子与累积总氨基酸比例在约0.4至1之间; 或v）组合的累积谷氨酰胺和约16至36mM之间的累积天冬酰胺浓度。 使用这样的系统允许高水平的蛋白质产生并减少某些不需要的因素例如铵和/或乳酸盐的积累。 此外，提供包括温度变化的培养方法，通常包括当培养物达到其最大细胞密度的约20-80％时的温度降低。 或者或另外，本发明提供了在达到峰值之后，培养物中的乳酸盐和/或铵水平随时间降低的方法。

    Production of TNFR-lg
    14.
    发明申请
    Production of TNFR-lg 有权
    生产TNFR-Ig

    公开(公告)号：US20060121569A1

    公开(公告)日：2006-06-08

    申请号：US11213633

    申请日：2005-08-25

    申请人： Denis Drapeau ,  Yen-Tung Luan ,  James Mercer ,  Wenge Wang ,  Daniel Lasko

    发明人： Denis Drapeau ,  Yen-Tung Luan ,  James Mercer ,  Wenge Wang ,  Daniel Lasko

    IPC分类号： C12P21/06 ,  C12N5/06

    CPC分类号： C12P21/02 ,  C07K14/7151 ,  C07K2319/30 ,  C12N2500/32 ,  C12N2500/90 ,  C12N2500/95 ,  C12P21/00

    摘要： An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.

    摘要翻译： 特别是在以下一个或多个特征的介质中大规模生产蛋白质和/或多肽的改进系统：i）累积的氨基酸浓度大于约70mM; ii）累积谷氨酰胺的累积天冬酰胺摩尔比小于约2; iii）累积谷氨酰胺与累积总氨基酸的摩尔比小于约0.2; iv）摩尔累计无机离子与累积总氨基酸比例在约0.4至1之间; 或v）组合的累积谷氨酰胺和约16至36mM之间的累积天冬酰胺浓度。 使用这样的系统允许高水平的蛋白质产生并减少某些不需要的因素例如铵和/或乳酸盐的积累。 此外，提供包括温度变化的培养方法，通常包括当培养物达到其最大细胞密度的约20-80％时的温度降低。 或者或另外，本发明提供了在达到峰值之后，培养物中的乳酸盐和/或铵水平随时间降低的方法。

    Use of copper and glutamate in cell culture for production of polypeptides
    16.
    发明授权
    Use of copper and glutamate in cell culture for production of polypeptides 有权
    在细胞培养中使用铜和谷氨酸来生产多肽

    公开(公告)号：US09012180B2

    公开(公告)日：2015-04-21

    申请号：US12040392

    申请日：2008-02-29

    申请人： Denis Drapeau ,  Jessica Snow ,  Gregory Hiller ,  Yen Tung Luan

    发明人： Denis Drapeau ,  Jessica Snow ,  Gregory Hiller ,  Yen Tung Luan

    IPC分类号： C12P21/04 ,  C12N5/00

    CPC分类号： C12N5/0018 ,  C12N2500/20 ,  C12N2500/32 ,  C12N2510/02

    摘要： An improved system for large scale production of polypeptides in cell culture is provided. In accordance with the present invention, cells expressing a polypeptide of interest are grown in media that contain copper, glutamate or both. The use of such a system allows production of polypeptides in which misfolding and/or aggregation are reduced, and in which total glycosylation is increased. Polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, agricultural or other commercial compositions.

    摘要翻译： 提供了用于在细胞培养物中大规模生产多肽的改进体系。 根据本发明，表达感兴趣的多肽的细胞在含有铜，谷氨酸或两者的培养基中生长。 使用这种系统允许产生其中错误折叠和/或聚集降低的多肽，并且其中总糖基化增加。 根据本发明表达的多肽可有利地用于制备药物，农业或其它市售组合物。

    Use of Copper Glutamate in Cell Culture for Production of Polypeptides
    17.
    发明申请
    Use of Copper Glutamate in Cell Culture for Production of Polypeptides 有权
    在细胞培养中使用谷氨酸铜用于生产多肽

    公开(公告)号：US20090068705A1

    公开(公告)日：2009-03-12

    申请号：US12040392

    申请日：2008-02-29

    申请人： Denis Drapeau ,  Jessica Snow ,  Gregory Hiller ,  Yen Tung Luan

    发明人： Denis Drapeau ,  Jessica Snow ,  Gregory Hiller ,  Yen Tung Luan

    IPC分类号： C12P21/00 ,  C12N5/02

    CPC分类号： C12N5/0018 ,  C12N2500/20 ,  C12N2500/32 ,  C12N2510/02

    摘要： An improved system for large scale production of polypeptides in cell culture is provided. In accordance with the present invention, cells expressing a polypeptide of interest are grown in media that contain copper, glutamate or both. The use of such a system allows production of polypeptides in which misfolding and/or aggregation are reduced, and in which total glycosylation is increased. Polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, agricultural or other commercial compositions.

    摘要翻译： 提供了用于在细胞培养物中大规模生产多肽的改进体系。 根据本发明，表达感兴趣的多肽的细胞在含有铜，谷氨酸或两者的培养基中生长。 使用这种系统允许产生其中错误折叠和/或聚集降低的多肽，并且其中总糖基化增加。 根据本发明表达的多肽可有利地用于制备药物，农业或其它市售组合物。

    Production of anti-abeta
    18.
    发明授权
    Production of anti-abeta 有权
    生产抗菌素

    公开(公告)号：US07335491B2

    公开(公告)日：2008-02-26

    申请号：US11213317

    申请日：2005-08-25

    申请人： Denis Drapeau ,  Yen-Tung Luan ,  James R. Mercer ,  Wenge Wang ,  Daniel R. Lasko

    发明人： Denis Drapeau ,  Yen-Tung Luan ,  James R. Mercer ,  Wenge Wang ,  Daniel R. Lasko

    IPC分类号： C12P21/06

    CPC分类号： C07K16/28 ,  C07K16/00 ,  C07K16/18 ,  C07K16/22 ,  C07K2319/30 ,  C12N5/0018 ,  C12N2500/20 ,  C12N2500/32 ,  C12N2510/04 ,  C12P21/00

    摘要： An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.

    摘要翻译： 特别是在以下一个或多个特征的介质中大规模生产蛋白质和/或多肽的改进系统：i）累积的氨基酸浓度大于约70mM; ii）累积谷氨酰胺的累积天冬酰胺摩尔比小于约2; iii）累积谷氨酰胺与累积总氨基酸的摩尔比小于约0.2; iv）摩尔累计无机离子与累积总氨基酸比例在约0.4至1之间; 或v）组合的累积谷氨酰胺和约16至36mM之间的累积天冬酰胺浓度。 使用这样的系统允许高水平的蛋白质产生并减少某些不需要的因素例如铵和/或乳酸盐的积累。 此外，提供包括温度变化的培养方法，通常包括当培养物达到其最大细胞密度的约20-80％时的温度降低。 或者或另外，本发明提供了在达到峰值之后，培养物中的乳酸盐和/或铵水平随时间降低的方法。