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公开(公告)号：US07335491B2
公开(公告)日：2008-02-26
申请号：US11213317
申请日：2005-08-25
申请人： Denis Drapeau , Yen-Tung Luan , James R. Mercer , Wenge Wang , Daniel R. Lasko
发明人： Denis Drapeau , Yen-Tung Luan , James R. Mercer , Wenge Wang , Daniel R. Lasko
IPC分类号： C12P21/06
CPC分类号： C07K16/28 , C07K16/00 , C07K16/18 , C07K16/22 , C07K2319/30 , C12N5/0018 , C12N2500/20 , C12N2500/32 , C12N2510/04 , C12P21/00
摘要： An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.
摘要翻译： 特别是在以下一个或多个特征的介质中大规模生产蛋白质和/或多肽的改进系统：i）累积的氨基酸浓度大于约70mM; ii）累积谷氨酰胺的累积天冬酰胺摩尔比小于约2; iii）累积谷氨酰胺与累积总氨基酸的摩尔比小于约0.2; iv）摩尔累计无机离子与累积总氨基酸比例在约0.4至1之间; 或v）组合的累积谷氨酰胺和约16至36mM之间的累积天冬酰胺浓度。 使用这样的系统允许高水平的蛋白质产生并减少某些不需要的因素例如铵和/或乳酸盐的积累。 此外，提供包括温度变化的培养方法，通常包括当培养物达到其最大细胞密度的约20-80％时的温度降低。 或者或另外，本发明提供了在达到峰值之后，培养物中的乳酸盐和/或铵水平随时间降低的方法。
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公开(公告)号：US5760189A
公开(公告)日：1998-06-02
申请号：US464176
申请日：1995-06-02
申请人： Steven M. Vicik , Neil L. Schauer , James R. Mercer , Edward R. LaVallie , Catherine A. Briasco , Jeffrey S. Deetz , Dwight Winters , Jenifer L. Thomas
发明人： Steven M. Vicik , Neil L. Schauer , James R. Mercer , Edward R. LaVallie , Catherine A. Briasco , Jeffrey S. Deetz , Dwight Winters , Jenifer L. Thomas
IPC分类号： C12N15/09 , C07K1/18 , C07K1/30 , C07K1/36 , C07K14/54 , C07K19/00 , C12N9/02 , C12N15/62 , C12P21/02 , C12R1/19 , C07K1/14 , C07K1/32
CPC分类号： C12N9/0036 , C07K1/18 , C07K1/30 , C07K14/5431 , C07K19/00 , C12N15/62 , C12P21/02 , C07K2319/00 , C07K2319/35 , C07K2319/50 , C07K2319/75
摘要： Provided by the present invention are novel methods of protein recovery and purification methods and more specifically novel methods for the recovery and purification thioredoxin fusion proteins, especially of IL-11.
摘要翻译： 本发明提供蛋白质回收和纯化方法的新方法，更具体地说，用于回收和纯化硫氧还蛋白融合蛋白，特别是IL-11的新方法。
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公开(公告)号：US07300773B2
公开(公告)日：2007-11-27
申请号：US11213633
申请日：2005-08-25
申请人： Denis Drapeau , Yen-Tung Luan , James R. Mercer , Wenge Wang , Daniel R. Lasko
发明人： Denis Drapeau , Yen-Tung Luan , James R. Mercer , Wenge Wang , Daniel R. Lasko
IPC分类号： C12P21/06
CPC分类号： C12P21/02 , C07K14/7151 , C07K2319/30 , C12N2500/32 , C12N2500/90 , C12N2500/95 , C12P21/00
摘要： An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.
摘要翻译： 特别是在以下一个或多个特征的介质中大规模生产蛋白质和/或多肽的改进系统：i）累积的氨基酸浓度大于约70mM; ii）累积谷氨酰胺的累积天冬酰胺摩尔比小于约2; iii）累积谷氨酰胺与累积总氨基酸的摩尔比小于约0.2; iv）摩尔累计无机离子与累积总氨基酸比例在约0.4至1之间; 或v）组合的累积谷氨酰胺和约16至36mM之间的累积天冬酰胺浓度。 使用这样的系统允许高水平的蛋白质产生并减少某些不需要的因素例如铵和/或乳酸盐的积累。 此外，提供包括温度变化的培养方法，通常包括当培养物达到其最大细胞密度的约20-80％时的温度降低。 或者或另外，本发明提供了在达到峰值之后，培养物中的乳酸盐和/或铵水平随时间降低的方法。
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公开(公告)号：US4044925A
公开(公告)日：1977-08-30
申请号：US677520
申请日：1976-04-16
申请人： James R. Mercer
发明人： James R. Mercer
CPC分类号： B29C45/54
摘要： A power-retractable feeder for an injection molding machine in which an actuator is adapted to forcibly seal a button-type outlet nozzle of the feeder against a side inlet port of a charging cylinder without developing an unbalanced lateral load on the cylinder. As disclosed, the reaction force developed on the actuator is transmitted to the side of the cylinder opposite the inlet. The actuator is selectively operable to retract the feeder nozzle from the cylinder for inspection, removal, maintenance, or the like. The disclosed feeder is of the screw type driven by a rotary motor through a coupler which isolates an axial reaction force on the screw from the motor. Also disclosed is a remote actuator and associated linkage for operating a shut-off valve in the feeder nozzle at either the extended or retracted position of the nozzle.
摘要翻译： 一种用于注塑机的动力可伸缩进料器，其中致动器适于将进料器的按钮式出口喷嘴强制地密封在充气筒的侧入口上，而不会在气缸上形成不平衡的横向载荷。 如所公开的，在致动器上产生的反作用力被传递到与入口相对的气缸侧。 致动器可选择性地可操作以将进料器喷嘴从气缸中缩回以进行检查，去除，维护等。 所公开的进料器是由旋转电机通过耦合器驱动的螺杆型，其将螺杆上的轴向反作用力与电机隔离。 还公开了一种远程致动器和相关连接件，用于在喷嘴的延伸或缩回位置处操作供料嘴中的截止阀。
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公开(公告)号：US3989434A
公开(公告)日：1976-11-02
申请号：US626841
申请日：1975-10-29
申请人： James R. Mercer
发明人： James R. Mercer
CPC分类号： B29C45/581 , B01F5/0656
摘要： Mixing means in the form of a torpedo for an injection molding machine having a plurality of alternately isolated and intersecting flow paths for repeatedly dividing and recombining material flow streams to achieve uniform properties throughout the material. The paths are formed as right and left-hand helical channels on the circumference of the torpedo body, with the helixes of one hand having a pitch different from that of the other such that the flow paths intersect in a pattern which is bilaterally asymmetrical with respect to an axial net flow direction. The nonsymmetrical arrangement is formed to provide substantially greater mixing action than that obtainable with similar symmetrical arrangements.
摘要翻译： 用于注射成型机的鱼雷形式的混合装置具有多个交替隔离和相交的流动路径，用于重复地分离和重新组合材料流动流以在整个材料中实现均匀的性质。 这些路径形成为在鱼雷体的圆周上的左右螺旋通道，其中一只手的螺旋线具有与另一只手的螺旋不同的螺距，使得流动路径以相对于两侧不对称的图案相交 到轴向净流动方向。 形成非对称布置以提供比具有相似对称布置可获得的更大的混合作用。
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公开(公告)号：US4645354A
公开(公告)日：1987-02-24
申请号：US833536
申请日：1986-02-26
申请人： James R. Mercer
发明人： James R. Mercer
CPC分类号： G04B49/02
摘要： A heliochronometer (10) for providing accurate clock time readouts at any known latitude and longitude location on the earth including, a base (15) to effect precision orientation and level mounting and having an unpstanding post (40), a latitude member (45) adjustably angularly positionable relative to the post for selecting a particular latitude, a gnomon assembly (65) extending upwardly from the latitude member, a longitude correction member (75) adjustably pivotally mounted about the gnomon assembly for effecting a longitude correction, a time dial support (20) pivotally mounted about said gnomon assembly and having an arcuate time dial (150) on which the shadow of the gnomon assembly is projected, and assemblies (90, 130) interconnecting the longitude correction member and the time dial support for adjusting the angular position of the time dial for daylight savings time and the equation of time.
摘要翻译： 一种用于在地球上的任何已知纬度和经度位置提供准确的时钟时间读数的日历同步计（10），包括：基座（15），用于实现精确定向和水平安装并具有不理想的柱（40），纬度（45） 相对于柱子可调节地定位以选择特定的纬度，从纬度构件向上延伸的侏儒组件（65），可调整地枢转地安装在榫头组件周围的经度校正构件（75），用于进行经度校正，时间拨盘支撑 （20），其围绕所述齿母组件枢转地安装并且具有弧形时间盘（150），所述齿形组件的阴影在其上突出;以及组合件（90,130），其连接所述经度校正构件和所述时间拨盘支撑件， 夏令时间拨号位置和时间方程。
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公开(公告)号：US4017242A
公开(公告)日：1977-04-12
申请号：US626303
申请日：1975-10-28
申请人： James R. Mercer
发明人： James R. Mercer
CPC分类号： B29C45/73 , B29C45/2725 , B29C2033/023 , B29C2045/2733 , B29K2101/10
摘要： A thermosetting injection molding machine includes an injector chamber, a distribution platen, four distribution nozzles, and a mold heating platen. A thermosetting material flows from the injector chamber to two crossed distribution chambers each of which extends from edge to edge completely through the distribution platen. Liquid coolant passages extend through the distribution platen along both sides of each of the distribution chambers. The distribution nozzles include cores which block the ends of the distribution chambers and which carry the thermosetting material through the mold heating platen to the mold. The distribution nozzles also include jackets having a greater coefficient of heat conductivity than the cores for maintaining all but the tip of the cores adjacent the mold at a temperature below the curing temperature of the thermosetting material. A removable mold for the machine includes a mold cluster adjacent each of the four distribution nozzles. The mold clusters each include a plurality of mold cavities and a runner for carrying the thermosetting material from its associated distribution nozzle to its associated mold cavities. By this arrangement, a multiple nozzle distribution system and a mold heating system are provided by the machine completely independently of the mold to simplify mold design.
摘要翻译： 热固性注射成型机包括注射器室，分配台板，四个分配喷嘴和模具加热台板。 热固性材料从注射器室流到两个交叉的分配室，每个分配室从边缘到边缘完全延伸通过分布压板。 液体冷却剂通道沿着每个分配室的两侧延伸通过分配压板。 分配喷嘴包括封闭分配室的端部并且将热固性材料通过模具加热板传送到模具的芯。 分配喷嘴还包括具有比芯部更大的导热系数的夹套，用于在低于热固性材料的固化温度的温度下保持与模具相邻的芯部的除了顶端之外的全部。 用于机器的可移除模具包括邻近四个分配喷嘴中的每一个的模具组。 模具簇各自包括多个模腔和用于将热固性材料从其相关联的分配喷嘴承载到其相关联的模具腔的流道。 通过这种布置，机器完全独立于模具提供多个喷嘴分配系统和模具加热系统，以简化模具设计。
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公开(公告)号：US07294484B2
公开(公告)日：2007-11-13
申请号：US11213308
申请日：2005-08-25
申请人： Denis Drapeau , Yen-Tung Luan , James R. Mercer , Wenge Wang , Daniel R. Lasko
发明人： Denis Drapeau , Yen-Tung Luan , James R. Mercer , Wenge Wang , Daniel R. Lasko
IPC分类号： C12P21/06
CPC分类号： C12P21/02 , C07K16/18 , C07K16/22 , C07K16/2896 , C07K2317/24 , C12N5/0037 , C12N2500/22 , C12N2500/32 , C12N2500/38 , C12N2500/60 , C12N2510/02
摘要： An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.
摘要翻译： 特别是在以下一个或多个特征的介质中大规模生产蛋白质和/或多肽的改进系统：i）累积的氨基酸浓度大于约70mM; ii）累积谷氨酰胺的累积天冬酰胺摩尔比小于约2; iii）累积谷氨酰胺与累积总氨基酸的摩尔比小于约0.2; iv）摩尔累计无机离子与累积总氨基酸比例在约0.4至1之间; 或v）组合的累积谷氨酰胺和约16至36mM之间的累积天冬酰胺浓度。 使用这样的系统允许高水平的蛋白质产生并减少某些不需要的因素例如铵和/或乳酸盐的积累。 此外，提供包括温度变化的培养方法，通常包括当培养物达到其最大细胞密度的约20-80％时的温度降低。 或者或另外，本发明提供了在达到峰值之后，培养物中的乳酸盐和/或铵水平随时间降低的方法。
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