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公开(公告)号：US20060118711A1
公开(公告)日：2006-06-08
申请号：US11283912
申请日：2005-11-22
IPC分类号： H01J49/00
CPC分类号： H01J49/40 , H01J49/0004 , Y10T436/24
摘要： In-plane distribution of a target objectcontained in a sample is measured. The sample dispersedly placed on a substrate is treated to promote ionization of the target object, then the mass and flying amount of an ion containing the target object or a component of the target object is determined by irradiating an ion beam to the sample and performing time-of-flight secondary ion mass spectrometry of the ion that flies from a portion in the sample where the ion beam is irradiated, and the in-plane distribution of the target object is determined from the mass and flying amount data obtained at plural portions by scanning the beam on the sample plane. The step of treating the sample to promote ionization of the target object includes contacting an aqueous solution of an acid that does not crystallize at ordinary temperature with the sample. A high spatial resolution two-dimensional image can be obtained.
摘要翻译： 测量样本中所包含的目标对象的平面内分布。 处理分散放置在基板上的样品以促进目标物体的电离，然后通过向样品照射离子束并执行时间来确定含有目标物体或目标物体的成分的离子的质量和飞行量 从照射离子束的样品中的部分飞散的离子的飞行二次离子质谱法，以及目标物体的面内分布根据多个部分得到的质量和飞行量数据来确定， 扫描样品平面上的光束。 处理样品以促进目标物体的电离的步骤包括使常温下不结晶的酸的水溶液与样品接触。 可以获得高空间分辨率的二维图像。
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公开(公告)号：US09086404B2
公开(公告)日：2015-07-21
申请号：US11867276
申请日：2007-10-04
CPC分类号： G01N33/5082 , G01N35/1002 , G01N2035/1041
摘要： A method is provided by which a reagent is provided to a living sample, including contacting a thin-film containing a porous body with a living sample containing cells or tissues, and discharging a solution containing at least one or more reagents to a non-contact side of the thin-film by ink-jet to provide the solution to the specific area of the living sample through pores on the thin-film, wherein the smaller diameter of the pores on the porous body relative to the diameter of cells in the living sample enables the reagent to be provided to each individual cell separately. According to this method, the reagent and the reaction product can be fixed while maintaining positioning information of the target substance in the living sample, as a pretreatment for analyzing with high positioning accuracy the target substance in each individual cell in the living sample.
摘要翻译： 提供了一种将试剂提供给活体样品的方法，包括使含有多孔体的薄膜与含有细胞或组织的活体样品接触，并将含有至少一种或多种试剂的溶液排出至非接触 通过喷墨使薄膜的一侧通过薄膜上的孔提供到活体样品的特定区域的溶液，其中多孔体上的孔的直径相对于活体中细胞的直径较小 样品使得可以分别向每个单独的细胞提供试剂。 根据该方法，可以在维持活体样品中的目标物质的定位信息的同时固定试剂和反应产物，作为用于以高定位精度分析活体样品中各个细胞中的目标物质的预处理。
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公开(公告)号：US20140087408A1
公开(公告)日：2014-03-27
申请号：US13995645
申请日：2011-11-28
IPC分类号： G01N33/574
CPC分类号： G01N33/57415 , C07K14/4756 , G01N33/6848 , G01N2333/4756
摘要： The present invention relates to an information acquisition method of acquiring information of distribution of a protein or peptide in a sample based on mass information obtained by mass spectrometry of the protein or peptide. The method includes mass spectrometry of a definite region of the sample after limited proteolysis of the protein or peptide and acquisition of information relating to distribution using an ion peak that has a two-dimensional intensity distribution having a Pearson product-moment correlation coefficient of 0.5 to more and 1.0 or less in the definite region against the two-dimensional intensity distribution of the parent ion of the protein or the peptide subjected to the limited proteolysis and has a peak intensity ratio of larger than 1.0 against the peak intensity of the integrated spectrum of the parent ion in the definite region, wherein the m/z of the ion peak is greater than 500.
摘要翻译： 本发明涉及基于通过蛋白质或肽的质谱法获得的质量信息获取样品中蛋白质或肽的分布信息的信息获取方法。 该方法包括在蛋白质或肽的有限蛋白水解之后样品的确定区域的质谱，并且使用具有Pearson积矩相关系数为0.5的二维强度分布的离子峰获取与分布有关的信息 相对于蛋白质或经受有限蛋白水解的肽的母离子的二维强度分布，确定区域中的1.0和更小，并且具有大于1.0的峰强度比与峰积分的峰强度比 该定子区域中的母离子，其中离子峰的m / z大于500。
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公开(公告)号：US20090266982A1
公开(公告)日：2009-10-29
申请号：US12427583
申请日：2009-04-21
CPC分类号： H01J49/0418 , Y10T436/24 , Y10T436/25 , Y10T436/25875
摘要： A mass spectrometry method that makes it possible to perform high-sensitivity detection of a desorbed/ionized object substance to be measured in mass spectrometry in which the object substance to be measured is desorbed and ionized. The method includes placing at least an ionizing agent having two or more functional groups represented by Formula (1) below in a molecule: —(CF2)COOH (1) wherein the ionizing agent has a boiling point of equal to or higher than 150° C. and an object molecule to be measured on a substrate and irradiating the ionizing agent and the object molecule to be measured with a primary beam selected from ions, neutral particles, electrons, and a laser beam.
摘要翻译： 能够对被测定物被解吸和离子化的质谱法进行能够进行待测量的解吸附电离对象物质的高灵敏度检测的质谱法。 该方法包括在分子中至少放置一个具有两个或多个由式（1）表示的官能团的电离剂： - （CF 2）COOH（1）其中电离剂的沸点等于或高于150℃ 和待测量的物体分子，并用从离子，中性粒子，电子和激光束中选择的一次束照射被测量的电离剂和待测物体。
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15.发明授权Biological tissue processing substrate for fixing proteins or protein degradation products in tissue for analysis 有权用于将蛋白质或蛋白质降解产物固定在组织中用于分析的生物组织处理底物公开(公告)号：US08962302B2
公开(公告)日：2015-02-24
申请号：US11592313
申请日：2006-11-03
申请人： Kazuhiro Ban , Miki Ogawa , Norihiko Utsunomiya , Hiroyuki Hashimoto , Manabu Komatsu , Yohei Murayama
发明人： Kazuhiro Ban , Miki Ogawa , Norihiko Utsunomiya , Hiroyuki Hashimoto , Manabu Komatsu , Yohei Murayama
IPC分类号： C12M1/00 , C12M1/34 , C12Q1/00 , C12Q1/34 , C12Q1/37 , C12Q1/02 , C12N11/14 , C12N11/02 , C12N11/04 , G01N33/68 , G01N33/50
CPC分类号： G01N33/6848 , G01N33/5082
摘要： A biological tissue processing substrate for fixing proteins in a biological tissue or degradation products of the proteins, the substrate comprising: a porous body that forms a contact surface with the biological tissue, the porous body holding in pores an enzyme for obtaining the proteins or the degradation products of the proteins from the biological tissue, wherein the proteins or the degradation products obtained by the action of the enzyme are brought into contact with a member consisting of a metal.
摘要翻译： 一种用于将蛋白质固定在生物组织中的生物组织处理底物或蛋白质的降解产物，所述底物包括：与生物组织形成接触表面的多孔体，多孔体在孔中保留用于获得蛋白质的酶或 使来自生物组织的蛋白质的降解产物与由金属构成的构件接触，其中通过酶的作用获得的蛋白质或降解产物与金属接触。
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公开(公告)号：US08217340B2
公开(公告)日：2012-07-10
申请号：US12427583
申请日：2009-04-21
IPC分类号： H01J49/26
CPC分类号： H01J49/0418 , Y10T436/24 , Y10T436/25 , Y10T436/25875
摘要： A mass spectrometry method that makes it possible to perform high-sensitivity detection of a desorbed/ionized object substance to be measured in mass spectrometry in which the object substance to be measured is desorbed and ionized. The method includes placing at least an ionizing agent having two or more functional groups represented by Formula (1) below in a molecule: —(CF2)COOH (1) wherein the ionizing agent has a boiling point of equal to or higher than 150° C. and an object molecule to be measured on a substrate and irradiating the ionizing agent and the object molecule to be measured with a primary beam selected from ions, neutral particles, electrons, and a laser beam.
摘要翻译： 能够对被测定对象物质进行离子化和离子化的质谱法进行高灵敏度地检测待测量的解吸/电离对象物质的质谱法。 该方法包括在分子中至少放置一个具有两个或多个由式（1）表示的官能团的电离剂： - （CF 2）COOH（1）其中电离剂的沸点等于或高于150℃ 和待测量的物体分子，并用从离子，中性粒子，电子和激光束中选择的一次束照射被测量的电离剂和待测物体。
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17.发明申请公开(公告)号：US20090026364A1
公开(公告)日：2009-01-29
申请号：US12239344
申请日：2008-09-26
IPC分类号： B01D59/44
CPC分类号： H01J49/40 , H01J49/0004 , Y10T436/24
摘要： In-plane distribution of a target object contained in a sample is measured. The sample dispersedly placed on a substrate is treated to promote ionization of the target object. Then, the mass and flying amount of an ion containing the target object or a component thereof is determined by irradiating an ion beam to the sample and performing time-of-flight secondary ion mass spectrometry of the ion that flies from a portion in the sample where the ion beam is irradiated, and the in-plane distribution of the target object is determined from the mass and flying amount data obtained at plural portions by scanning the beam on the sample plane. The step of treating the sample to promote ionization of the target object includes contacting an aqueous solution of an acid that does not crystallize at ordinary temperature with the sample. A high spatial resolution two-dimensional image can be obtained.
摘要翻译： 测量包含在样本中的目标对象的平面内分布。 处理分散放置在基板上的样品，以促进目标物体的电离。 然后，通过向样品照射离子束并进行从样品的一部分飞出的离子的飞行时间二次离子质谱法来确定含有目标物体或其成分的离子的质量和飞散量 其中照射离子束，并且通过在样品平面上扫描光束从多个部分获得的质量和飞行量数据确定目标物体的面内分布。 处理样品以促进目标物体的电离的步骤包括使常温下不结晶的酸的水溶液与样品接触。 可以获得高空间分辨率的二维图像。
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公开(公告)号：US20080290268A1
公开(公告)日：2008-11-27
申请号：US12120512
申请日：2008-05-14
IPC分类号： B01D59/44
CPC分类号： H01J49/145
摘要： An information obtaining method for obtaining information about a mass of a component of an analyte using a time of flight mass spectrometer and obtaining information about a distribution state of the component based on the obtained information about the mass includes the step of: (1) adding a self-reactive substance to the analyte on a base to facilitate ionization of the component; (2) irradiating the analyte with a primary beam in the presence of the self-reactive substance, thereby ionizing the components and allowing resulting ions to fly; (3) obtaining information about mass of the flying ions using the time of flight mass spectrometer; and (4) obtaining information about the distribution state of the component on the base based on the information about the mass.
摘要翻译： 使用飞行时间质谱仪获取关于分析物的成分的质量的信息的信息获取方法，基于获得的关于质量的信息获得关于部件的分布状态的信息，包括以下步骤：（1）添加 对基底上的分析物的自反应物质，以促进组分的离子化; （2）在存在自反应物质的情况下用主光束照射分析物，从而电离成分并允许所得离子飞行; （3）使用飞行时间质谱仪获取关于飞行离子质量的信息; 和（4）基于关于质量的信息获得关于组件在基础上的分布状态的信息。
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公开(公告)号：US20080090267A1
公开(公告)日：2008-04-17
申请号：US11867276
申请日：2007-10-04
IPC分类号： C12Q1/02
CPC分类号： G01N33/5082 , G01N35/1002 , G01N2035/1041
摘要： A method is provided by which a reagent is provided to a living sample, including contacting a thin-film containing a porous body with a living sample containing cells or tissues, and discharging a solution containing at least one or more reagents to a non-contact side of the thin-film by ink-jet to provide the solution to the specific area of the living sample through pores on the thin-film, wherein the smaller diameter of the pores on the porous body relative to the diameter of cells in the living sample enables the reagent to be provided to each individual cell separately. According to this method, the reagent and the reaction product can be fixed while maintaining positioning information of the target substance in the living sample, as a pretreatment for analyzing with high positioning accuracy the target substance in each individual cell in the living sample.
摘要翻译： 提供了一种将试剂提供给活体样品的方法，包括使含有多孔体的薄膜与含有细胞或组织的活体样品接触，并将含有至少一种或多种试剂的溶液排出至非接触 通过喷墨使薄膜的一侧通过薄膜上的孔提供到活体样品的特定区域的溶液，其中多孔体上的孔的直径相对于活体中细胞的直径较小 样品使得可以分别向每个单独的细胞提供试剂。 根据该方法，可以在维持活体样品中的目标物质的定位信息的同时固定试剂和反应产物，作为用于以高定位精度分析活体样品中各个细胞中的目标物质的预处理。
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