公开(公告)号：US08962302B2

公开(公告)日：2015-02-24

申请号：US11592313

申请日：2006-11-03

申请人： Kazuhiro Ban ,  Miki Ogawa ,  Norihiko Utsunomiya ,  Hiroyuki Hashimoto ,  Manabu Komatsu ,  Yohei Murayama

发明人： Kazuhiro Ban ,  Miki Ogawa ,  Norihiko Utsunomiya ,  Hiroyuki Hashimoto ,  Manabu Komatsu ,  Yohei Murayama

IPC分类号： C12M1/00 ,  C12M1/34 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/02 ,  C12N11/14 ,  C12N11/02 ,  C12N11/04 ,  G01N33/68 ,  G01N33/50

CPC分类号： G01N33/6848 ,  G01N33/5082

摘要： A biological tissue processing substrate for fixing proteins in a biological tissue or degradation products of the proteins, the substrate comprising: a porous body that forms a contact surface with the biological tissue, the porous body holding in pores an enzyme for obtaining the proteins or the degradation products of the proteins from the biological tissue, wherein the proteins or the degradation products obtained by the action of the enzyme are brought into contact with a member consisting of a metal.

摘要翻译： 一种用于将蛋白质固定在生物组织中的生物组织处理底物或蛋白质的降解产物，所述底物包括：与生物组织形成接触表面的多孔体，多孔体在孔中保留用于获得蛋白质的酶或 使来自生物组织的蛋白质的降解产物与由金属构成的构件接触，其中通过酶的作用获得的蛋白质或降解产物与金属接触。

公开(公告)号：US20070105087A1

公开(公告)日：2007-05-10

申请号：US11592313

申请日：2006-11-03

申请人： Kazuhiro Ban ,  Miki Ogawa ,  Norihiko Utsunomiya ,  Hiroyuki Hashimoto ,  Manabu Komatsu ,  Yohei Murayama

发明人： Kazuhiro Ban ,  Miki Ogawa ,  Norihiko Utsunomiya ,  Hiroyuki Hashimoto ,  Manabu Komatsu ,  Yohei Murayama

IPC分类号： C12Q1/00 ,  C12M3/00

CPC分类号： G01N33/6848 ,  G01N33/5082

摘要： A biological tissue processing substrate for fixing proteins in a biological tissue or degradation products of the proteins, the substrate comprising: a porous body that forms a contact surface with the biological tissue, the porous body holding in pores an enzyme for obtaining the proteins or the degradation products of the proteins from the biological tissue, wherein the proteins or the degradation products obtained by the action of the enzyme are brought into contact with a member consisting of a metal.

摘要翻译： 一种用于将蛋白质固定在生物组织中的生物组织处理底物或蛋白质的降解产物，所述底物包括：与生物组织形成接触表面的多孔体，多孔体在孔中保留用于获得蛋白质的酶或 使来自生物组织的蛋白质的降解产物与由金属构成的构件接触，其中通过酶的作用获得的蛋白质或降解产物与金属接触。

公开(公告)号：US20080073509A1

公开(公告)日：2008-03-27

申请号：US11682500

申请日：2007-03-06

申请人： Kazuhiro Ban ,  Hiroyuki Hashimoto ,  Manabu Komatsu ,  Norihiko Utsunomiya ,  Yohei Murayama

发明人： Kazuhiro Ban ,  Hiroyuki Hashimoto ,  Manabu Komatsu ,  Norihiko Utsunomiya ,  Yohei Murayama

IPC分类号： B01D59/44

CPC分类号： H01J49/0418

摘要： A sample is sliced obliquely with a blade provided with a metal thin film on the surface, and the slice of the sample adhering to the blade surface is subjected to mass spectrometry. As a result, the section of the sliced sample can be analyzed immediately by mass spectrometry with high sensitivity.

摘要翻译： 将样品用表面上设置有金属薄膜的刀片倾斜切片，并且将附着在刀片表面上的样品切片进行质谱分析。 因此，可以通过高灵敏度的质谱立即分析切片样品的部分。

公开(公告)号：US07560689B2

公开(公告)日：2009-07-14

申请号：US11682500

申请日：2007-03-06

申请人： Kazuhiro Ban ,  Hiroyuki Hashimoto ,  Manabu Komatsu ,  Norihiko Utsunomiya ,  Yohei Murayama

发明人： Kazuhiro Ban ,  Hiroyuki Hashimoto ,  Manabu Komatsu ,  Norihiko Utsunomiya ,  Yohei Murayama

IPC分类号： B01D59/44 ,  H01J49/00 ,  H01J49/40 ,  G01N31/00 ,  G01N33/00 ,  G01N23/00 ,  G21K7/00

CPC分类号： H01J49/0418

摘要： A sample is sliced obliquely with a blade provided with a metal thin film on the surface, and the slice of the sample adhering to the blade surface is subjected to mass spectrometry. As a result, the section of the sliced sample can be analyzed immediately by mass spectrometry with high sensitivity.

摘要翻译： 将样品用表面上设置有金属薄膜的刀片倾斜切片，并且将附着在刀片表面上的样品切片进行质谱分析。 因此，可以通过高灵敏度的质谱立即分析切片样品的部分。

公开(公告)号：US20060279738A1

公开(公告)日：2006-12-14

申请号：US11448070

申请日：2006-06-07

申请人： Miki Ogawa ,  Norihiko Utsunomiya

发明人： Miki Ogawa ,  Norihiko Utsunomiya

IPC分类号： G01N21/55

CPC分类号： G01N21/554

摘要： A method for producing a sensing element used in a sensing device for sensing a target substance in a specimen with the use of plasmon resonance includes the steps of: preparing a substrate having an electroconductive layer, forming an insulating layer on the electroconductive layer, selectively removing the insulating layer to make the electroconductive layer appear, and depositing a metal on the appearing electroconductive layer with an electrolytic plating method.

摘要翻译： 一种在使用等离子体共振来检测样品中的目标物质的感测装置中使用的传感元件的制造方法，其特征在于，包括：制作具有导电层的基板，在导电层上形成绝缘层，选择性地除去 出现导电层的绝缘层，并用电解电镀法在出现的导电层上沉积金属。

公开(公告)号：US08023109B2

公开(公告)日：2011-09-20

申请号：US12895776

申请日：2010-09-30

申请人： Junta Yamamichi ,  Miki Ogawa ,  Yoichiro Handa ,  Takeshi Imamura ,  Norihiko Utsunomiya ,  Satoru Nishiuma

发明人： Junta Yamamichi ,  Miki Ogawa ,  Yoichiro Handa ,  Takeshi Imamura ,  Norihiko Utsunomiya ,  Satoru Nishiuma

IPC分类号： G01N21/27 ,  G01N21/64

CPC分类号： G01N21/05 ,  G01N21/554 ,  G01N21/645 ,  G01N21/66 ,  G01N2021/0346

摘要： A detection device comprising a substrate comprising a plurality of objects of which properties are changed due to the contact with a target substance, means for bringing the target substance into contact with the objects, and means for detecting a change in properties of the objects caused when the target substance is brought into contact with the objects, based on light output when the objects are irradiated with light, wherein the plurality of the objects are located in the direction in which the light for irradiation travels, and the detecting means is means for detecting the change in the properties based on the summation of light output from the plurality of the objects upon irradiation with light.

摘要翻译： 一种检测装置，包括：基板，包括由于与目标物质的接触而使特性变化的多个物体;使目标物质与物体接触的装置;以及用于检测物体的特性变化的装置， 当物体被光照射时，目标物质与物体接触，其中多个物体位于照射光的行进方向上，检测装置是检测装置 基于在照射光时来自多个物体的光输出的总和的属性的变化。

公开(公告)号：US20080297802A1

公开(公告)日：2008-12-04

申请号：US12125543

申请日：2008-05-22

申请人： Miki Ogawa ,  Norihiko Utsunomiya

发明人： Miki Ogawa ,  Norihiko Utsunomiya

IPC分类号： G01N21/55 ,  G01N1/00

CPC分类号： G01N21/554 ,  B01L3/5025 ,  B01L3/50857 ,  G01N2021/258

摘要： A detecting element and a detecting device having high sensitivity, and a production method thereof are provided. The detecting element for detecting a target substance in a sample includes a substrate and a metal structure. The substrate has a pore in which the thickness direction of the substrate corresponds to the depth direction of the pore and in which part of the substrate forming the pore constitutes part of the outer surface of the substrate. The metal structure exists in the pore, and has a ring shape.

摘要翻译： 提供了具有高灵敏度的检测元件和检测装置及其制造方法。 用于检测样品中的目标物质的检测元件包括基板和金属结构。 衬底具有孔，其中衬底的厚度方向对应于孔的深度方向，并且其中形成孔的衬底的一部分构成衬底的外表面的一部分。 金属结构存在于孔中，具有环状。

公开(公告)号：US20050244987A1

公开(公告)日：2005-11-03

申请号：US11114073

申请日：2005-04-26

申请人： Norihiko Utsunomiya ,  Kazuhiro Ban ,  Takashi Ikeda

发明人： Norihiko Utsunomiya ,  Kazuhiro Ban ,  Takashi Ikeda

IPC分类号： G01N33/483 ,  C12Q1/68 ,  G01N27/74 ,  G01N33/543 ,  G01N33/551 ,  G01N33/553 ,  G01R33/12

CPC分类号： G01N33/5438 ,  C12Q1/6816 ,  C12Q2563/143

摘要： A detection device for detecting a target substance in a specimen comprises a flow channel for delivering the specimen, a coil surrounding the flow channel, and a reaction region being placed in a portion of the flow channel surrounded by the coil outside and holding a first trap substance immobilized thereon for trapping the target substance, the coil bring about a change of a physical property thereof when the target substance is trapped by the first trap substance and a magnetic body having a second trap substance on the surface thereof which second trap substance is capable of specifically binding to the target substance is trapped by the target substance.

摘要翻译： 用于检测试样中的目标物质的检测装置包括用于输送试样的流路，围绕流路的线圈，以及反应区域，放置在由线圈外围包围的流路的一部分中，并保持第一捕集器 固定在其上用于捕获目标物质的物质，当目标物质被第一捕获物质捕获时，线圈引起其物理性质的变化，并且其表面上具有第二捕获物质的第二捕获物质的能力 与靶物质特异性结合的物质被目标物质捕获。

公开(公告)号：US20090009756A1

公开(公告)日：2009-01-08

申请号：US10571906

申请日：2005-03-02

申请人： Junta Yamamichi ,  Miki Ogawa ,  Yoichiro Handa ,  Takeshi Imamura ,  Norihiko Utsunomiya ,  Satoru Nishiuma

发明人： Junta Yamamichi ,  Miki Ogawa ,  Yoichiro Handa ,  Takeshi Imamura ,  Norihiko Utsunomiya ,  Satoru Nishiuma

IPC分类号： G01N21/01

CPC分类号： G01N21/05 ,  G01N21/554 ,  G01N21/645 ,  G01N21/66 ,  G01N2021/0346

摘要： A detection device comprising a substrate comprising a plurality of objects of which properties are changed due to the contact with a target substance, means for bringing the target substance into contact with the objects, and means for detecting a change in properties of the objects caused when the target substance is brought into contact with the objects, based on light output when the objects are irradiated with light, wherein the plurality of the objects are located in the direction in which the light for irradiation travels, and the detecting means is means for detecting the change in the properties based on the summation of light output from the plurality of the objects upon irradiation with light.

摘要翻译： 一种检测装置，包括：基板，包括由于与目标物质的接触而使特性变化的多个物体;使目标物质与物体接触的装置;以及用于检测物体的特性变化的装置， 当物体被光照射时，目标物质与物体接触，其中多个物体位于照射光的行进方向上，检测装置是检测装置 基于在照射光时来自多个物体的光输出的总和的属性的变化。

公开(公告)号：US07403287B2

公开(公告)日：2008-07-22

申请号：US11448070

申请日：2006-06-07

申请人： Miki Ogawa ,  Norihiko Utsunomiya

发明人： Miki Ogawa ,  Norihiko Utsunomiya

IPC分类号： G01N21/55

CPC分类号： G01N21/554

摘要： A method of producing a sensing element used in a detecting device for detecting a target substance in a specimen with the use of plasmon resonance. The method includes the steps of forming an insulating layer on a substrate, the surface of the substrate having an electroconductive layer including a first electroconductive material, forming a plurality of holes through the insulating layer to form an insulating pattern, filling up the holes with a second electroconductive material to form a metallic pattern, removing the insulating pattern, and removing a region other than the region sandwiched by the metallic pattern and the substrate in the electroconductive layer, using the metallic pattern as a mask.

摘要翻译： 一种用于检测装置中用于使用等离子体共振来检测样品中的目标物质的感测元件的方法。 该方法包括在衬底上形成绝缘层的步骤，衬底的表面具有包括第一导电材料的导电层，通过绝缘层形成多个孔以形成绝缘图案，用一个 第二导电材料以形成金属图案，去除绝缘图案，并且使用金属图案作为掩模，除去导电层中被金属图案和基板夹持的区域以外的区域。